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Citations for Thermo Fisher :
4751 - 4800 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... cells were plated into 6-well plates at the density of 300000 cells per well and 24h after plating treated with Aβ or p3 peptides diluted in OPTIMEM (Thermo Fisher Scientific) supplemented with 5% FBS (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: ... Aliquots containing 10 μg of peptide from each sample were labeled with tandem mass tag (TMT) 16-plex reagents (TMTpro, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Liquid chromatography-mass spectrometry (LC-MS) analyses of trypsin-digested peptides were performed on a Q Exactive HF-X hybrid Quadrupole-Orbitrap instrument (Thermo Fisher Scientific) coupled with an Easy-nLC 1200 system ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 120-min gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... Baker ™) and peptide concentrations were determined from their absorbance at 280 nm using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, USA).
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of tryptic peptides were used for High pH RP-HPLC using a 25 cm ProSwift™ RP-4H capillary monolithic column (Thermo Scientific) on an Agilent 1200 series HPLC (high-pressure liquid chromatography ...
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bioRxiv - Molecular Biology 2024Quote: 1.5 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Immunology 2024Quote: Unenriched peptides and enriched phosphopeptides from each fraction were analyzed by LC-MS/MS on an Orbitrap Exploris 480 (ThermoFisher, Bremen, Germany) connected to a Dionex 3700 RSLC Nano using 100 µm PicoFrit column self-packed with 2.2 μm Acclaim RSLC 120 C18 (ThermoFisher) ...
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bioRxiv - Genetics 2024Quote: ... The digested and derivatized histone peptides were diluted in 0.1% TFA and injected to nLC-QqQ MS (Dionex nanoLC and a ThermoFisher Scientific TSQ Quantum). Peptides were first loaded to a trapping column (2 cm × 150 μm ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions were spiked with iRT standard peptides and analyzed in technical duplicates via LC-MS/MS on a hybrid quadrupole-orbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific) hyphenated to a nanoflow chromatography systems (Easy-nLC 1000 UHPLC ...
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bioRxiv - Cell Biology 2024Quote: Peptides were analyzed by nano-HPLC-tandem mass spectrometry (MS/MS) using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific, Dreieich, Germany) connected to an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and peptides were analysed via a nanospray ion source into a Q Exactive Plus hybrid quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... (i) peptide separation using acetonitrile gradient was run for 120 min,(ii) data was collected on an Orbitrap Fusion (Thermo Fisher Scientific) operated at 120,000 resolution using HCD (higher-energy collisional dissociation ...
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A network medicine approach to elucidate mechanisms underlying menopause-induced knee osteoarthritisbioRxiv - Pathology 2023Quote: ... analysis of peptide fractions was carried out on an EASY-nLC 1000 coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific). Each fraction was loaded onto an Aurora 25cm x 75µm ID ...
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bioRxiv - Immunology 2023Quote: ... Peptides were ionized by electrospray after HPLC separation with a MS-coupled Proxeon EASY-nLC 1200 liquid chromatography system (Thermo Fisher Scientific) using 75 µm inner diameter nanocapillaries with PicoTip emitters (NewObjective # PF360-75-10-CE-5 ...
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bioRxiv - Microbiology 2023Quote: ... The concentration of peptides was determined by measuring UV absorbance at 280 nm or by performing Pierce rapid gold BCA protein assay (Thermo Fisher Scientific) in case of peptides lacking aromatic amino acids ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 and LTQ-Orbitrap Velos Pro, Thermo Fisher Scientific) using a 124 min gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were separated on an Acclaim PepMap C18 column (id 75 μm, length 50 cm, particle size 2 μm, Thermo Fisher Scientific) using a 10%-45% gradient of solvent B (80% acetonitrile ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng TMT-labelled peptide sample was measured on an EASY-nLC 1200 system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific, www.thermofisher.com). Liquid chromatography was performed using 20 cm fritless silica emitters with 0.75 µm inner diameter (New Objective ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting peptides were analyzed by nanoLC-MS/MS using an Ultimate 3000 RSLCnano coupled to a Q-Exactive HF (Thermo Fisher Scientific) using a 120-min gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... Eluted phosphopeptide samples from both enrichments were pooled and fractionated into 14 fractions by increasing ACN concentration from 7 % to 50 % using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC ...
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bioRxiv - Microbiology 2023Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Fisher Scientific) over a 150 min organic gradient ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... B31-Biotin labelled peptides were analyzed using Agilent 1100 nano pump coupled to an LTQ Velos Pro-Orbitrap Elite mass spectrometry (Thermo Scientific, USA). Sample was loaded onto a trap column consisting of a fritted capillary (360 μm o.d. ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting peptides were extracted and analyzed by high-sensitivity LC-MS/MS on an Orbitrap Fusion mass spectrometer (Thermo Scientific, Waltham MA). Proteins were identified by database searching of the fragment spectra against the SwissProt (EBI ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific). Peptide sequences (and hence protein identity ...
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bioRxiv - Molecular Biology 2023Quote: ... Fifty micrograms of peptides/sample were labeled with TMT labels (16-plex) according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA), and pooled ...
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bioRxiv - Plant Biology 2023Quote: ... each sample containing 25 μg of peptide in 50 mM TEAB buffer was combined with its respective 10-plex TMT reagent (Thermo Fisher Scientific) and incubated for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted peptides were dried and resuspended in 0.1% FA before injection LC-MS/MS analysis of the digested peptides was conducted using an Orbitrap Q Exactive Plus mass spectrometer coupled to an EASY-nLC 1200 (Thermo Fisher Scientific). A home-made column (C18 50 cm capillary column picotip silica emitter tip (75 μ diameter ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by matching protein databases with the acquired fragmentation pattern by Sequest (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Orbitrap Exploris 480, Thermo Fisher Scientific) using a 180-min gradient ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated using vacuum centrifugation and separated into eight fractions using Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (84868, Thermo Scientific) according to the manufacturer’s instructions for TMT-labelled peptides ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 140 min gradient ...
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bioRxiv - Microbiology 2023Quote: ... was added to cultures to label new peptide synthesis before harvesting (Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit, Invitrogen)88 ...
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bioRxiv - Systems Biology 2023Quote: Acquisition of single-cell derived peptides was conducted with an Orbitrap Astral mass spectrometer operated in positive mode with the FAIMS Pro interface (Thermo Fisher Scientific) using a compensation voltage set to −50 V ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Microbiology 2023Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (2 μm, 75 μm x 25 cm, Thermo Fisher Scientific) at 35 °C in either 60-minute or 90-minute gradients of 5% to 35% buffer B (98% ACN with 0.1% formic acid ...
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bioRxiv - Microbiology 2023Quote: ... LC-MS/MS analysis of the biotinylated peptides was performed on an EASY-nLC 1200 UHPLC connected to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) as described previously67 ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were injected into C18 nano-column on an EASY-nLC (Proxeon) coupled with an Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). A ninety-minute gradient was used for separation at a flow rate of 300nL/minutes ...
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bioRxiv - Cell Biology 2023Quote: Samples were prepared for mass spectrometry as previously detailed.19 Freeze-dried peptides were resuspended in 80 µl of distilled water and quantified by the BCA assay (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... The concentration of peptide conjugates was determined by the standard Bicinchoninic acid (BCA) assay using Pierce(tm) BCA Protein Assay Kit (Thermo Fisher Scientific). To confirm successful synthesis of peptide conjugates ...
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bioRxiv - Microbiology 2022Quote: ... Generated peptides were analysed by nanoLC-MS/MS on a QExactive□+□mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific, USA). The data were searched against a database containing the Human Swissprot sequences (2017_01) ...
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bioRxiv - Systems Biology 2023Quote: ... 20 to 30 µg of proteins were digested in-gel and the resulting peptides were analysed online using a Q Exactive HF mass spectrometer coupled with an Ultimate 3000 RSLC system (Thermo Fisher Scientific). MS/MS analyses were performed using the Maxquant software (v1.5.5.1 ...
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bioRxiv - Cell Biology 2022Quote: Lyophilized peptide pellets were reconstituted in 100 mM TEAB and concentration estimates were performed spectroscopically using a Nanodrop 1000 (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL min-1) and identity of peptides confirmed by high-resolution mass (Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific).
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bioRxiv - Genetics 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using 120 min and 90 min acetonitrile gradients for SIN-3 and ARID-1 interactomes respectively ...
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bioRxiv - Microbiology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by comparing the fragmentation pattern acquired by the software program Sequest (Thermo Fisher Scientific) (64 ...
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bioRxiv - Microbiology 2023Quote: ... Eluted peptides were subjected to electrospray ionization and entered into an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific). Peptides were detected ...
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bioRxiv - Molecular Biology 2023Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (2 µm, 75 µm x 25 cm, Thermo Fisher Scientific) at 35 °C in a 90 min gradient of 5% to 40% buffer B (98% ACN with 0.1% formic acid ...