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Citations for Thermo Fisher :
5051 - 5100 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Evolutionary Biology 2022Quote: A 2-μg aliquot of the peptide mixture was analyzed using a LTQ-Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... approximately 1.5 μg of peptide per sample was injected into Ultra-High-Performance Liquid Chromatography (UHPLC) system (Ultimate™ 3000 RSLCnano, (Thermo Fisher Scientific, USA) coupled online with Impact HD™ UHR-QqToF (Bruker Daltonics ...
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bioRxiv - Plant Biology 2022Quote: Coding sequences of mature proteins without signal peptide were amplified with primers listed in Table S1 and cloned into expression vector pETSUMO (Thermo Fisher Scientific, Massachusetts, USA). Correctness of the resulting constructs pETSUMO-ChElp2 and pETSUMO-Vd2LysM were confirmed by DNA sequencing and introduced into E ...
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bioRxiv - Plant Biology 2021Quote: A small fraction of all samples were pooled and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Pierce, Thermo Fisher Scientific, San Jose). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Immunology 2020Quote: ... The pool of peptides generated in the degradation assay was assessed by mass spectrometry using a Q-Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) and nano-HPLC (Dionex Ultimate 3000 ...
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bioRxiv - Systems Biology 2022Quote: The resulting peptides were dried to 12 µL and analyzed by nanoLC-MS/MS using a Dionex Ultimate 3000 (Thermo Fisher Scientific, Bremen, Germany) coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of peptide material of each sample was injected for LC-MS/MS analysis on an Ultimate 3000 RSLC nano-LC (Thermo Fisher Scientific, Bremen, Germany) in-line connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... brain-derived tryptic peptides were separated using a NanoAcquity UHPLC (Waters, Milford, FA) and monitored on a Q-Exactive Plus mass spectrometer (ThermoFisher Scientific, San Jose, CA). Raw data were analyzed using MaxQuant v1.5.2.8 with Thermo Foundation 2.0 for RAW file reading capability17 ...
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bioRxiv - Biochemistry 2020Quote: ... The peptides were collected and desalted on Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany) and stored at −80°C.
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bioRxiv - Microbiology 2020Quote: ... Peptides were extracted from the gels and analysed by LC-MS/MS on an Orbitrap-Fusion™ mass spectrometer (Thermo Fisher, Hemel Hempstead, UK) equipped with an UltiMate™ 3000 RSLCnano System using an Acclaim PepMap C18 column (2 µm ...
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bioRxiv - Microbiology 2020Quote: ... The extracted peptides from OE and OB groups were labeled with TMT10 reagents according to the manufacturer’s instructions (Thermo Scientific, San Jose, CA, USA), respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... The peptides were desalted by an HLB column (Waters, Milford, MA) and were then drained by a vacuum pump (Thermo Fisher Scientific, Bremen, Germany). The peptides were acidified with 0.1% formic acid and were loaded onto a reversed-phase microcapillary column using a Thermal EASY-n LC1200 chromatography system ...
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bioRxiv - Cell Biology 2020Quote: Nanoflow LC–MS/MS analysis of tryptic peptides was conducted on a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Bremen, Germany)14 coupled to an EASY nLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion was stopped by the addition of 2% (v/v) formic acid before peptides were cleaned using Pierce C18 spin columns (Thermo Fisher Scientific, cat#89870) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were then directly labeled as follows: acetonitrile was added to 30% (v/v) concentration and peptides were labeled with TMT 10-plex reagent (Thermo Fisher Scientific, Catalog #90406) for 1 hour ...
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bioRxiv - Physiology 2020Quote: ... Antibodies to the antigen peptide were purified from the final bleed antiserum by affinity-purification using the AminoLink Plus Immobilization Kit (ThermoFisher Scientific, Waltham, MA, USA), with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals ...
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bioRxiv - Genetics 2021Quote: ... The separated peptides were loaded onto a C18 column (50 cm × 75 μm) connected to an EASY-nLC™ 1200 system (Thermo Fisher Scientific, USA) and separated with a linear gradient of buffer B (80% acetonitrile and 0.1% formic acid ...
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bioRxiv - Biochemistry 2021Quote: Separation of digested histone peptides was performed by using nanoliter flow liquid chromatography using an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, CA, USA) equipped with an analytical weak cation exchange–hydrophilic interaction liquid chromatographic resin (PolycatA ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of peptide was injected into an Ultimate 3000 RSLC chromatography system connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific, Waltham, MA, USA). Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm ...
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bioRxiv - Biochemistry 2020Quote: Peptides and cross-linked peptides were analyzed using an UltiMate3000 RSLCnano (Dionex, Sunnyvale, CA) coupled to an Orbitrap Fusion (Thermo-Fisher Scientific, San Jose, CA) mass spectrometer equipped with a nanospray Flex Ion Source ...
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bioRxiv - Developmental Biology 2022Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... and fragmented to produce a tandem mass-spectrum of specific fragment ions for each peptide with LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... the RBD was inserted into pcDNA3.4 with a streptavidin binding peptide (SBP) tag at the C-terminus and were produced using the Expi293 Expression System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Neuroscience 2024Quote: LC-MS/MS analysis for DDA Peptides from each pooled fraction were first spiked with iRT standard peptides (Biognosys AG, Switzerland) and separated using reverse-phase high-performance liquid chromatography (RP-HPLC) on EASY-nLC system (Thermo Fisher Scientific, Bremen, Germany) with a column (75 μm × 150 mm ...
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bioRxiv - Cancer Biology 2024Quote: Tandem Mass Tag (TMT)-10 labeling of peptides was conducted as follows: The TMT-10 plex Isobaric Label Reagent (Catalog No. 90110, Thermo Fisher Scientific, Waltham, MA) was dissolved in anhydrous acetonitrile (ACN ...
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bioRxiv - Microbiology 2024Quote: ... followed by a peptide clean-up on SPE spin columns before TMT labeling with the TMTpro™ 16plex Label Reagent Set (Thermo Fisher, Cat# A44520). Peptides from each sample were labeled with a distinct TMTpro™ isobaric tag before mixing ...
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bioRxiv - Bioengineering 2023Quote: ... the peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, MA, USA). Separation was performed on a Dionex EASY-Spray 75 μm × 10 cm column packed with PepMap C18 3 μm ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic peptides extracted from each IP product were separated by reverse-phased nano-flow liquid-chromatography (EASY-nLC II, Thermo Scientific, San Jose, CA) and analyzed on an LTQ/Orbitrap Velos Elite hybrid mass spectrometer (Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Eluted peptides were subjected to electrospray ionization and then entered into an LTQ Orbitrap Velos Elite ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Cell Biology 2023Quote: Peptide concentrations of whole cell S-trap extracts in 100 mM TEAB were quantified using the Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... each peptide sample (approximately 2 mg) was tapplied to a Pierce High-Select™ TiO2 Phosphopeptide Enrichment Kit (Thermo Fisher Scientific, Cat No: A32993). After preparing spin tips as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were reconstituted in 0.1% trifluoroacetic acid and fractionated on Sep-Pak® Vac cartridges using methodology and reagents from Pierce™ High pH reversed-phase peptide fractionation kit (8 fractions for global proteomics and 4 for phosphoproteomics skipping every other; Thermo Fisher Cat No: 84868). Samples were run (1/8th of each global and 1/5th of each phosphopeptide fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... the peptides were analyzed by online nanoflow liquid chromatography-tandem mass spectrometry performed on an EASY-nanoLC 1200 system (Thermo Fisher Scientific, MA, USA) connected to a Q Exactive™ Plusmass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and washed for 3 min at 30 μL/min before peptides were eluted onto a C18 Acclaim™ PepMap™ column (100 Å, 5 μm × 0.75 mm × 150 mm, Thermo Fisher Scientific) at a flow rate of 0.3 μL/min ...
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bioRxiv - Biochemistry 2023Quote: ... as three full experimental technical replicates (n=54) with ∼100 ng of peptides injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: For the identification of the peptides a Dionex Ultimate 3000 RSLCnano System coupled to an Orbitrap Eclipse Tribrid Mass Spectrometer (both Thermo Fisher Scientific, Vienna, Austria) was used.
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bioRxiv - Immunology 2023Quote: The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were loaded on an Acclaim PepMap 100 μ-precolumn cartridge (5 μm, 100 Å, 300 μm ID x 5 mm, Thermo Fisher Scientific) and separated at 40°C on a PicoTip emitter (noncoated ...
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bioRxiv - Biophysics 2024Quote: ... Degraded ProteaseMAXTM was removed via centrifugation (max. speed, 10 minutes) and the peptide solid phase extracted using 10 µl volume C18 tips (Pierce○R (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the peptide mixture was split into two equal aliquots of 1 mg each and 350 fmol of standard AQUA QuantPro peptides (Thermo Fisher Scientific, Waltham, USA) were added to each aliquot before K-ε-GG peptide enrichment ...
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bioRxiv - Synthetic Biology 2024Quote: ... Peptides were loaded onto a C18 Acclaim PepMap NanoLC column (25 cm length, 75 µm inner diameter, Thermo Fisher Scientific, San Jose, CA, USA) packed with 2 µm particles and a 100 Å pore size ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated at room temperature for 1 hour in a dark humid chamber with goat anti- mouse IgG-Alexa488 (Invitrogen, A11001, 1:500 in blocking buffer). Cells were washed 4 more times with blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... sections were washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and then incubated for 1 hour in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...