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Citations for Thermo Fisher :
5101 - 5150 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and then incubated in secondary antibodies diluted with the blocking solution (1:100 for goat anti-mouse Alexa 633 (Thermo Fisher Scientific #A-21052, RRID: AB_2535719), 1:100 for goat anti-chicken Alexa 488 (Thermo Fisher Scientific #A-11039 ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed four times in PBS and incubated with goat anti-rabbit antibody conjugated with AlexaFluor594 for 1 hour at room temperature (Invitrogen; 1:200 dilution in blocking reagent). 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2019Quote: ... the cells were incubated with goat anti-rabbit or anti-mouse IgG antibody conjugated with Alexa Fluor 488 dye or Alexa Fluor 568 dye (Life Technologies; 1000x dilution in blocking solution) for 60 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Triton-X-100 and 5% (v/v) normal goat sera) for 30 min prior to staining with a rabbit anti-HA polyclonal antibody (Invitrogen, diluted 1:100 in blocking buffer) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with the blocking buffer and incubated at room temperature with fluorophore-conjugated secondary antibodies (goat anti-mouse IgG Alexa 488, Thermo Fisher, Cat #: A11001, 1:500 dilution) (goat anti-rabbit IgG Alexa 555 ...
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bioRxiv - Bioengineering 2024Quote: ... Blotting took place with the LI-COR system (LI-COR 927-66003 [TBS-based blocking and diluent buffers] and ThermoFisher 28360 TBS-Tween 20 wash buffer) for imaging on a LI-COR Odyssey instrument ...
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bioRxiv - Cancer Biology 2024Quote: ... the mesenteries were incubated with a secondary antibody consisting of a blocking buffer solution and Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific, A-11008, 1:1000) for 1 hour in a darkroom then washed 3 times with PBS for 10 minutes and mounted on microscopic slides ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... before secondary antibody (Donkey α-Rabbit, Alexa Fluor 488, Invitrogen #A21206, 1:2000; Goat α- mouse, Alexa Fluor 488, Invitrogen #A11001, 1:2000 in blocking buffer) was applied to the sections and incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... blocked in 3% normal donkey serum/0.25% Triton X100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rabbit anti-GFP, 488-conjugated, Novus #NB600-308, 1:5000; rabbit anti-TH, Thermo Fisher Scientific #OPA1-04050, 1:5000). The next morning ...
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bioRxiv - Microbiology 2023Quote: ... Resulting biofilms were first washed with 1x PBS to remove loosely-bound cells and incubated with BSA blocking buffer at room temperature for 0.5 h (Thermo Scientific, 3% BSA is PBS; J61655.AK). Biofilms were then probed with two murine monoclonal antibodies against B-DNA (Anti-dsDNA ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated in the dark for 30 min at room temperature with secondary antibodies diluted 1:800 in blocking buffer: donkey anti-rabbit AlexaFluor 488 (Thermo Fisher Scientific, Catalog No. A-21206) and donkey anti-goat AlexaFluor 555 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... treated with a 10% fetal bovine serum (FBS)/PBS blocking solution (Fisher bioreagents, catalog #BP9703-100) and stained for eIF5A1/2 polyclonal antibody (Thermo Fisher Sci. catalog #PA529204, 1:200) or SMAD3 (Cell Signaling Technology catalog #9523S ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed three times at 5 minutes each with DPBS before incubation for 1 hour at room temperature in the dark with secondary antibodies in blocking buffer (goat anti-rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher, 1:200 dilution, Cat # A-11008) or goat anti-mouse IgG1 Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... sections were washed two times for 8 min in DPBS containing 0.3% Triton X100 and then incubated for 1 hr in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... peptide samples were purified via Stage tips using punched out cookies of C18 3M™ Empore™ extraction disc material (Fisher Scientific #14-386-2) as matrix ...
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bioRxiv - Cell Biology 2022Quote: Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: Nanoflow LC–MS/MS analysis of tryptic peptides was conducted on a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Rockford, IL, USA) [48] coupled to an EASY nLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides ionized with electrospray ionization (ESI) and analyzed in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously (Verberkmoes et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The tryptic peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, San Jose, CA) as previously described (Gallart-Palau et al. ...
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bioRxiv - Cancer Biology 2021Quote: Peptides from all experiments were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Fisher Scientific, Waltham, MA, USA), using a binary gradient with buffer A (2% acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were loaded at 5 μL/min onto a C18 trap column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective ...
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bioRxiv - Biophysics 2020Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Neuroscience 2021Quote: ... Briefly, peptides were loaded onto a Thermo NanoViper trap column (75 µm x 20 mm, 3 µm C18, 100 Å) (Thermo Fisher Scientific, Bremen, Germany) using an Agilent 1260 Infinity nanoLC system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... using an EASY-nLC 1200 system (peptides were loaded and separated on a 30 cm long home-made C18 column; Thermo Fisher Scientific, Waltham, MA, USA) coupled to a Q Exactive Plus system (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Four μl of the different peptide mixtures (800 ng for yeast peptides and 949 ng for bulks) were analyzed using an Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... these peptides were loaded onto the Xbridge BEH300 C18 column (Waters Milford, MA, USA) for HPLC (UltiMate 3000 UHPLC; Thermo Fisher Scientific, Waltham, MA, USA) separation ...
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bioRxiv - Biochemistry 2021Quote: ... For the FAIMS CV scanning of synthetic peptides and glycopeptides, each sample (Table S1, Supporting Information) was directly injected to the Orbitrap Lumos (Thermo Fisher Scientific, San Jose, CA). The CV was scanned from 0-100 V in 1 V steps ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Microbiology 2019Quote: ... Samples were resuspended in 20 µL 0.1 % formic acid (FA) and peptides were quantified by measuring the absorbance at 280 nm using a Nanodrop 1000 device (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2020Quote: ... Acidified eluted peptides were analyzed in the data-independent acquisition mode on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) online coupled to an ultra-high-performance liquid chromatography (UHPLC ...
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bioRxiv - Cell Biology 2022Quote: ... One aliquot containing 800 ng of Bradford-quantified peptides was analyzed on an ETD-enabled Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) connected to the EASY-nLC system (Proxeon Biosystem ...
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bioRxiv - Neuroscience 2022Quote: ... labeled peptides on one spin tip from a High-Select™ TiO2 Phosphopeptide Enrichment Kit (capacity of 1–3 mg; Thermo Fisher Scientific, catalog A32993). After preparing spin tips ...
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bioRxiv - Genetics 2022Quote: ... Sodium deoxycholate was removed by acidification with 10 % TFA and the sets were desalted using Pierce peptide desalting spin columns (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... the resulting peptides were labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled.
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bioRxiv - Pharmacology and Toxicology 2022Quote: The eluted peptides were ionized under high voltage (2 kV) and analyzed online using a LumosTM mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). The initial MS spectrum (MS1 ...
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bioRxiv - Cell Biology 2023Quote: Peptides were analyzed by LC-MS on a Dionex Ultimate HPLC coupled to an Orbitrap QE Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operating in Data Dependent Acquisition (DDA ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg of the peptide was used for LC-MS/MS analysis which was carried out using a nano-LC 1200 system (Thermo Fisher Scientific, San Jose, CA) coupled to Orbitrap Fusion™ Lumos ETD mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 25 cm EasySpray™ C18 column (2 μm, 100 Å, 75 μm x 25 cm, Thermo Scientific Cat No: ES902A) at 400 nL/min with a gradient of 4-30% with mobile phase B (Mobile phases A ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tryptic peptides from protein digests obtained from cell lines and tissue lysates were quantified by BCA using a peptide digest standard (Thermo Scientific, catalog no. 23225; 23295). Peptides were analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously [77 ...
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bioRxiv - Immunology 2023Quote: ... Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 6 µL of each sample and separated by high pH reversed phase peptide separation kit (Thermo Fisher Scientific, Waltham, MA, USA). Ten fractions were collected by centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000, Occludin mAb #33-1500 Invitrogen 1:500). On the next day the membranes were washed three times for tenminutes in Tris-buffered saline + 0.05% Tween (#91414 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed three times in PBS and incubated during 4 h in a blocking solution containing a secondary antibody to reveal TH (donkey anti-rabbit Alexa Fluor 594, Invitrogen A21207 lot 1890862 and 2145022 (1:400), RRID ...
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bioRxiv - Molecular Biology 2022Quote: ... The slips were washed three times with PBS before the complementary anti-rabbit secondary antibody was added (Cat#A-11034, Life Technologies; 1:1000 dilution in blocking solution) and incubated for one hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat. A16110; Invitrogen cat. 31432; Invitrogen cat. 31402; R&D Biosystems cat. HAF016). We performed a final TBST wash (4 x 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... TFA and 1 μL of this dilution and 1μL of the undiluted peptides from bands 3 and 4 were injected onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm × 5 mm), using a partial loop injection ...
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bioRxiv - Biochemistry 2021Quote: Samples from imaging peptide extraction were dissolved in 0.1% FA and analyzed on the Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to a Dionex UPLC system ...