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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for Rat CCAAT Enhancer Binding Protein Alpha CEBPa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: The cell membrane protein samples were collected using Mem-PER eukaryotic membrane protein extraction reagent kit (Thermo Scientific, 89826, MA) according to the protocol instruction ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysates were cleared by centrifugation at 13,000 rpm for 15 min and protein content was analyzed by Pierce BCA protein assay (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Subcellular protein fractionation was performed either by using Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific; Cat. no. 78840) according to the manufacturer protocol or following the protocol below.
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bioRxiv - Cell Biology 2020Quote: ... The eluted proteins were separated on a 4-12% NuPAGE gel (Novex) and stained with Imperial protein stain (Thermo Scientific). The proteins of interest were excised for identification by MS.
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bioRxiv - Cell Biology 2019Quote: Cells were lyzed using RIPA buffer and proteins were quantified using the BCA method (Protein BCA Assay Kit, Thermo Fisher). The protein extracts were separeted on 12% SDS-PAGE gels (50 μg of total proteins were loaded per well ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: ... Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) measured at 562 nm and compared to a standard curve ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was transferred to a new tube and total protein was quantified using EZQ® Protein Quantitation kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Protein in testes and cultured cells were extracted using T-PER Tissue protein extraction reagent (Thermo Fisher Scientific, Waltham, Massachusetts), subjected to SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were pelleted and 50μg of protein was quantified by ionic detergent compatible Pierce 660nm Protein Assay Reagent (Thermo Fisher) in 8M Urea ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein content of each pre-cleared sample was determined using Pierce bicinchoninic acid assay protein assay (Thermo Fisher Scientific). To measure luciferase activity ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were then measured by bicinchoninic acid (BCA) assay (Micro BCA™ Protein Assay Kit, Cat # 23235; Thermo Scientific). The resulting supernatant yielded low volumes (30-40 µl of supernatant ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentrations of the final purified samples were measured with Pierce™BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were separated on a NuPAGE™ 4–12% Bis-Tris Protein Gel (Thermo Fisher Scientific, Bleiswijk, the Netherlands) and transferred to an Immobilon-FL PVDF membrane (Merck ...
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bioRxiv - Genomics 2019Quote: ... Soluble material was incubated with 3-5 μg of antibody bound to 50 μl protein A or protein G Dynabeads (Invitrogen) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was determined relative to a BSA standard using the PierceTM BCATM Protein-Assay (Thermo Scientific, Waltham, MA, USA). Lysates were finally adjusted to a final concentration of 2 µg/µl with 4X Laemmli buffer (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... and CCL21 levels were normalized to total protein concentration of the homogenates determined using Pierce BCA protein Assay kit (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... Purity was determined by SDS-PAGE and protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). For generation of POM1 mutants ...
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bioRxiv - Genetics 2020Quote: ... gRNA/Cas9 complexes were formed by incubating 250 ng of the gRNA with 1 µg of purified Cas9 protein (TrueCut Cas9 Protein v2, Invitrogen) in OptiMEM (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... The lysate/antibody mixture was then incubated with either Protein A (for Flag-Riplet experiments) or Protein G Dynabeads (Invitrogen) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The amount of protein in each sample was determined using a BCA protein assay Kit (Thermo Scientific, Waltham, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were then clarified by spinning at 14,000 rpm at 4°C and protein concentration quantified by BCA Protein Assay (23227, Thermo Scientific). Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093 ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Samples were pre-cleared with Dynabeads Protein A and Dynabeads Protein G (Catalog #10001D and #Catalog #10003D, Thermo Fisher Scientific) for 1hr at 4 °C first and then incubated with rabbit anti-PntP1 antibody (a gift of J ...
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bioRxiv - Immunology 2021Quote: Total protein concentrations in BAL fluid were detected by Pierce BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured using Pierce BCA Protein Assay kit according to manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). 20 µg of total protein/lane were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were adjusted in 5xLDS sample buffer containing 50 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: Stability of VH1 proteins was determined with a Protein Thermal Shift Dye kit (Life Technologies Corporation, Grand Island, NY, USA) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of proteins was measured by the Pierce microBCA Protein Assay according to the manufacturer’s instruction (Thermo Fisher Scientific). Equal amounts of protein for each sample were precipitated with six volumes of pre-chilled (−20 °C ...
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bioRxiv - Biochemistry 2020Quote: Protein biosynthesis assays were carried out using the click-it plus O-propargyl-puromycin (OPP) protein synthesis assay (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations of membrane fractions in collected supernatant samples were measured by BCA protein assay kit (Thermo Fisher Scientific 23225).
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bioRxiv - Genomics 2021Quote: ... 50 µg of proteins from worm lysate and 10 µg of proteins from secretome were resolved on a 12% SDS-PAGE with a BenchMark protein ladder (Invitrogen) and a negative control (SDS-PAGE loading buffer and water) ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein concentration of the supernatants was determined using Pierce 660 nm Protein Assay Reagent Kit (22660, Thermo Fisher Scientific) supplemented with Ionic Detergent Compatibility Reagent (22663 ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant of cell lysates was assayed for total protein content using the BCA protein assay (Thermo Fisher Scientific, Rockford, IL), and 70 μg of total protein per sample was separated in a 7.5% polyacrylamide gel (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Labeled proteins were recovered from equivalent amounts of protein by batch affinity chromatography with streptavidin-coupled M280 Dynabeads (Thermo Fisher), 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... the protein concentration of each fraction was determined using a bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific). Samples were stored at −80 °C until further use.
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bioRxiv - Cell Biology 2021Quote: ... 5–12 µl of protein lysate was loaded into pre-cast protein gels (4–12 % Bolt Bis Tris Plus, ThermoFisher) together with 10 µl of the molecular marker (PageRuler prestained ...
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bioRxiv - Systems Biology 2020Quote: ... 20 μg of protein from each sample was separated on NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific) and transferred to nitrocellulose membranes (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration of the elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) and the elution fraction with the highest protein concentration was chosen for further use.
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bioRxiv - Neuroscience 2020Quote: Approximately 10μg of each protein extract was separated on a precast gradient gel (NuPAGE 4-12% Bis-Tris Protein Gels, NP0336BOX, Thermofisher) and transferred to PVDF membrane (GE10600022 ...
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bioRxiv - Microbiology 2021Quote: Protein content of samples for SDS-PAGE and immunoblotting was analyzed using a Pierce BCA Protein Assay (Thermo Scientific, Germany) with a standard curve from 0 to 2000 µg mL−1 bovine serum albumin following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... protein was extracted from supernatants and pellets were resuspended directly in protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein content of all extracts was determined using the Pierce BCA Protein Assay Kit (Cat no. 23225, ThermoFisher Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was re-suspended in 8M urea/50mM Tris pH8 and protein concentration was determinate with Qubit Protein Assay (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... phosphatase inhibitors) and proteins concentrations were measured using a Pierce BCA protein Assay Kit (Thermo Fisher Scientific, Ref: 23225/23227). 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples (50µg total protein) were resolved by SDS-PAGE using 4-12% Bis-Tris gel (Nupage, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was centrifuged at 10,000×g for 10 min and quantified by BCA Protein Assay Kit (Thermo Fisher Scientific). The same quantity (about 15 μg ...