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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for Rat CCAAT Enhancer Binding Protein Alpha CEBPa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 5,000 xg and protein concentration was quantified by Pierce BCA Protein Assay (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected and protein concentration determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Sci., Cat. no: 23225).
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Systems Biology 2023Quote: ... The protein concentration was determined with a BCA protein Assay kit following the instructions from the vendor (23225, Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged for 5 minutes at 16,000 x g and supernatant protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
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bioRxiv - Molecular Biology 2023Quote: Bacterially expressed and purified NEMO proteins were labeled with the Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher, A30006) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected into a new 1.5 ml tube and protein concentration was measured using the bovine serum albumin protein standard assay (Cat.No.: 23208, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein concentration of the cell lysates was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher, Cat. No. 23227). Cell lysates were mixed with the sample buffer (with beta mercaptanol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were obtained using the BCA Protein Assay Kit (Pierce) and 6X Laemmli SDS sample buffer (Thermo Fisher Scientific) was added to protein extracts ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected in a new tube and protein concentrations were measured using a BCA protein assay kit (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... 600 μg of chromatin was precleared with 30 μl of Protein A- or Protein G-coupled magnetic beads (Invitrogen Dynabeads) for one hour at 4°C on a rotating platform ...
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bioRxiv - Genetics 2023Quote: ... the concentration of each protein fraction was quantified in duplicate using a Pierce BCA protein assay kit (Thermo Scientific #23225) and results were read on a Synergy H1 Microplate Reader using BioTek Gen5-3.04 software per manufacturer instructions.
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bioRxiv - Immunology 2023Quote: ... 55,087 protein count) and contaminant protein database (provided by the manufacturer) in Proteome Discoverer software version 2.3 (Thermo Scientific, USA). A maximum of two trypsin cleavages were allowed with a precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.8 Da ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein samples were resolved on 4-12% NuPAGE Bis-Tris gel with protein molecular weight standards (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermofisher). The gels were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration in the lysates were determined by using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific #23225). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: To detect protein via western blotting 60µg of protein was resolved on SDS-PAGE 4- 12% Bis-Tris Bolt gels (Life Technologies). Protein was transferred onto a nitrocellulose membrane using a Mini Trans-Bolt Cell (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μl were saved at −80ºC for posterior determination of protein content using Pierce BCA Protein Assay Kit (Cat. # 23227, Thermo Scientific). Samples were then immediately heat-inactivated and cooled down to room temperature.
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bioRxiv - Developmental Biology 2023Quote: ... The total protein concentration in the cell lysate was quantified by using BCA protein Assay kit (Thermo Scientific™, 23225).
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bioRxiv - Immunology 2023Quote: ... Protein concentrations were measured for each sample using the A280 NanoDrop method and a protein standard curve (ThermoFisher Cat. 23208). Afterwards ...
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bioRxiv - Immunology 2023Quote: Quantification of total protein levels from BAL fluid was measured using a BCA protein assay kit (Thermo Fisher Cat# 23227) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total protein concentrations in membrane fractions were quantified using the Pierce™ BCA protein assay kit (ThermoFisher Scientific, Waltham, MA). For Western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... the protein concentration of the cell lysates was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). Equal amounts of total protein (1 mg ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by electrophoresis at 16 mA followed by transferring the proteins to Immobilon-P membranes (Thermo Fisher Scientific) for 1 hour at 350 mA (4°C ...
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bioRxiv - Cancer Biology 2023Quote: Total protein was quantified using the Pierce bicinchoninic protein assay (BCA) kit in accordance with manufacturer’s instructions (23225, ThermoFisher Scientific). Following protein quantification ...
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bioRxiv - Neuroscience 2023Quote: ... transfected cell lysates (500 µg total protein in 200 µl) were pre-cleared by incubating with 10 µl Dynabeads protein G (Invitrogen) at 4°C for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
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bioRxiv - Cancer Biology 2023Quote: ... the amount of protein per sample was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat. 23225). Trypsin digestion ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a mouse anti-biotin primary antibody at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... each sample’s total protein concentration was determined using a Pierce™ BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Biochemistry 2023Quote: ... The degree of labeling of the proteins with the fluorescent probes was evaluated by following the manufacturer’s protocol (Microscale Protein Labeling Kit, ThermoFisher Scientific).
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration in the supernatant was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: Nascent protein synthesis was measured with the Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit (cat. no. C10428, ThermoFisher). Cells were plated in 12-well plates (Cell Treat ...
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bioRxiv - Systems Biology 2023Quote: We quantified Protein synthesis rate with the “Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit” (Thermo Fisher Scientific), with minor adaptations from the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a primary antibody at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration of the CF was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: The protein concentration of the cell lysates was determined with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For co-immunoprecipitation ...
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bioRxiv - Developmental Biology 2023Quote: Membrane proteins were extracted from cell pellets using the Mem-PER™ Plus Membrane Protein Extraction Kit (89842, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Cell Biology 2023Quote: Protein size separation of 25 µg of protein was done on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, NP0336BOX) in NuPAGE MES SDS-buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... The plate was gently agitated for 20 minutes at room temperature and the protein concentration was measured using a Pierce BCA Protein Assay Kit (cat. #0023227, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... The final protein concentration was measured with the Micro BCA Protein Assay Kit (#OI191202, Thermo Fisher Scientific, Waltham, MA, USA). Protein purity was verified by SDS-PAGE and silver staining (Fig ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Lysate inputs were normalized by determining overall protein concentration using Pierce™ BCA Protein Assay (Thermo Fisher Scientific, Waltham, MA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... 1% of input chromatin was saved and 70 μg of chromatin pre-clearing was done by mixing with pre-blocked Sepharose beads (1:1 vol. mix of Protein A and protein G beads; Invitrogen) for 1h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The protein content of the samples was quantified using the Pierce™ 660 nm protein assay kit (Thermo Fisher, US) with BSA as the standard ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 M sucrose) and a protein concentration assay was performed (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia). 100 μg of isolated mitochondria were collected and centrifuged at 12,000 g for 15 min at 4 °C and after removal of the supernatant the pellet was frozen at −80 °C for subsequent proteomics analysis.
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were quantified via a BCA assay using the Pierce BCA Protein Assay Kit obtained from Life Technologies (Catalog #23225). Cell lysates were run through a 4-12% SDS-PAGE gel electrophoresis at 140-170V ...