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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for Rat CCAAT Enhancer Binding Protein Alpha CEBPa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Protein concentration of each qEV fraction was estimated by BCA protein assay according to the manufacturer’s protocol (ThermoFisher Scientific). Technical replicates by serial dilution were performed.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total BAL protein was quantified with the Qubit Total Protein Quantification kit and the Qubit 2.0 fluorometer (Thermo Scientific). BAL cytokines were profiled using a pre-mixed MILLIPLEX protein immunoassay (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentrations of the cell lysates were determined with a BCA protein assay kit (Thermo Fisher Scientific, USA). The cell lysates were pre-cleaned by an off-target control antibody and aliquoted ...
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bioRxiv - Neuroscience 2019Quote: ... Protein content was quantified using the Pierce™ BCA Protein Assay kit according to manufacturer protocols (Thermo Fisher Scientific). 20 μg of protein lysate was run on 4-20% Mini-PROTEAN TGX Precast Gel (BioRad) ...
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bioRxiv - Developmental Biology 2019Quote: Protein concentrations for individual samples were determined using a BCA protein assay following the manufacturer’s protocol (Thermo Scientific Pierce). 50 μg of protein were labeled with Alexa Fluor 555 and all reactions were quenched using 2M Tris (pH 6.8 ...
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bioRxiv - Synthetic Biology 2020Quote: Protein quantification was performed using the protocol and supplies provided by the BCA Protein Assay Kit (Thermo Scientific Pierce). Samples of BSA protein in PBS ranging in concentration from 25 ug/ml to 2000 ug/ml were used to create the standard concentration curve ...
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bioRxiv - Genetics 2020Quote: ... the supernatant was used to assess protein concentration using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific, #23225) before subsequent analysis ...
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bioRxiv - Genomics 2021Quote: ... The protein concentration of supernatant lysates was determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific) and a NanoDrop1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants from each filter were transferred into eppendorfs and protein concentrations were determined using Pierce Protein Quantitation Assay (ThermoFisher). Protein concentrations across samples were normalized to 4 mg/ml using 50mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µL were used to measure protein concentration by a BCA protein assay kit (Thermo Fisher Scientific, Roskilde, Denmark).
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bioRxiv - Systems Biology 2019Quote: ... The protein concentration in the cell lysate was quantified with a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, UK) using the provided albumin as standard ...
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bioRxiv - Synthetic Biology 2019Quote: ... The total protein concentration of MCP samples was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific) per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysate protein concentration was determined using Pierce BCA protein assay kit (Cat#23225; Lot no. QI223168; ThermoFisher Scientific). 50 μg of cell lysate was loaded onto a 4-12% gradient gel and ran at 165 V constant for 45-60 min in 1X MOPS-SDS buffer (NuPAGE ...
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bioRxiv - Plant Biology 2019Quote: ... About 20 µg of total soluble proteins of each sample was loaded to 3-12% Bis-Tris 1.0 mm Invitrogen NativePAGETM protein gels from ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were denatured at 95°C for 10min and protein quantity was measured using Pierce 660nm Protein Assay (ThermoFisher). Protein extracts (10µg ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 100 ug of protein was used per sample ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was measured by Qubit Protein Assay Kit on the Qubit fluorometer (Thermo Fisher scientific, Waltham, MA, USA). PO assay was performed by a modified Alaux protocol (Alaux et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... The concentration of total protein was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Equal amounts of total protein were separated electrophoretically on an SDS-PAGE gel ...
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bioRxiv - Cell Biology 2021Quote: Total protein was extracted using RIPR lysis buffer (KeyGEN, China) and quantified using the BCA protein assay (Thermo Scientific). The proteins of tissue samples were resolved by 10% SDS-PAGE and transferred to the PVDF membrane (0.2 µm pore size ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration was measured by Pierce BCA protein assay kit (Cat: 23228 from Thermo Fisher Scientific Inc, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration in each sample was determined using BCA protein assay kit (Cat no. Thermo Fisher Scientific, Massachusetts, USA) and equal amount of proteins were incubated with “MyOne Streptavidin C1” magnetic beads (Cat no ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... and the protein content of the thymic lysates was measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... Env proteins bound to magnetic beads were separated from unbound proteins using a DynaMag 15 magnet (Life Technologies, Inc.). Env protein bound beads were further incubated with blocking buffer (PBS [pH 7.4] ...
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bioRxiv - Microbiology 2021Quote: ... Final absolute nucleotide concentrations were normalized to total protein concentrations using the Pierce 660 nm Protein Assay (Thermo Scientific). Extraction experiments were repeated on separate days using two biological replicate cultures for each strain and temperature condition ...
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bioRxiv - Neuroscience 2020Quote: The cell surface proteins of hippocampus were isolated using Pierce cell surface protein isolation kit (Pierce, Thermo Scientific, MA). Biotinylated proteins bound to NeutrAvidin beads (bound fraction ...
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bioRxiv - Neuroscience 2022Quote: ... An aliquot from each supernatant was used to quantify total protein using the BCA Protein Assay Kit (ThermoFisher Scientific). The rest of the supernatant was filtered using an amicon-ultra© centrifuge filter with a cut-off of 3KD filter (Millipore) ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of each sample were determined using the Pierce™ BCA Protein Assay Kit (#23225, Thermo Fisher Scientific) according to the manufacturer’s protocol using the Photometer Tecan Infinite®200M (Tecan).
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bioRxiv - Molecular Biology 2022Quote: ... supernatant containing 300 μg protein was pre-cleaned with 100 μl protein G dynabeads (Thermo Fisher Scientific Biosciences, 10009D) for 30 min at 4°C on the overhang shaker ...
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Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteinsbioRxiv - Biochemistry 2022Quote: ... 4 μl of the flow through were subjected to a protein determination (Pierce BCA Protein Assay (Thermo Scientific, #23225). 25 μl of the remaining flow-through were mixed 1:1 with 2x sample buffer (180 mM Tris pH 6.8 ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein quantity was used for normalization using the Pierce® BCA Protein Assay Kit (Life Technologies, Carlsbad, CA).
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bioRxiv - Biochemistry 2022Quote: Protein samples were quantified using Pierce™ 660 Protein Assay Kit (plus Ionic Detergent Compatibility Reagent, IDCR; Thermo Scientific). Then ...
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bioRxiv - Cell Biology 2022Quote: ... and the concentration of the soluble protein fraction was estimated using a Pierce BCA protein assay kit (ThermoFisher Scientific). Protein lysates (15-20μg ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was quantitated using a BCA assay (Pierce™ BCA Protein Assay Kit, 23225, Thermo Fisher Scientific, USA). 10 ug of protein was loaded onto precast gel (4561095 ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg protein of each sample was loaded into 4 to 12% Bis-Tris protein gel (Thermo Fisher Scientific) and sent for MS analysis at the University of Texas System Proteomics Network ...
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bioRxiv - Cell Biology 2022Quote: Nascent protein synthesis rate was measured using Click-iT AHA Alexa Flour 488 protein synthesis HCS assay kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: Protein synthesis rate was monitored by the Click-iT Plus OPP Protein Synthesis Assay (Molecular Probes, Thermo Fisher Scientific). 200,000 cells were incubated with 20 µM Click-iT OPP (O-propargyl-puromycin ...
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bioRxiv - Physiology 2022Quote: ... Results were normalized to protein quantified using a bicinchoninic acid-based protein assay (Thermo Fisher Scientific, Waltham, MA, USA) in each well.
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bioRxiv - Physiology 2022Quote: Protein synthesis rate was monitored by the Click-iT Plus OPP Protein Synthesis Assay (Molecular Probes, Thermo Fisher Scientific). 200,000 cells were incubated with 20 µM Click-iT OPP (O-propargyl-puromycin ...
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bioRxiv - Neuroscience 2022Quote: ... A protein molecular weight marker was also loaded (PageRuler Plus Prestained Protein Ladder, Cat# 26619, Thermo Fisher Scientific Inc.). After rinsing ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were extracted from CEC or FFE cells in M-Per™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were analyzed for protein content by Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). SDS-PAGE and Westernblot were carried out by standard procedures ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein concentration in each sample was determined using the BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The protein fractions were quantified using an 8 point Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat.# PI23225), and 20 µg of protein per sample diluted to 35µL using NuPAGE LDS sample buffer (1x final conc. ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitation of the protein-antibody conjugate was performed incubating with Dynabeads® Protein A (Thermo Scientific, Carlsbad, CA, USA) 40 minutes at 4ºC in a rotary shaker ...
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bioRxiv - Microbiology 2023Quote: ... Eluted proteins from subcellular fractions were quantified by a Pierce ™ BCA protein assay kit (Cat.no: 23227. Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration in total cell lysates was determined by a BCA protein assay kit (Thermo Fisher Scientific, PI23227). Equal amounts of protein samples were loaded on 10% glycine-SDS-PAGE gel ...
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bioRxiv - Physiology 2023Quote: ... protein concentrations in detergent-soluble cell lysates were determined using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific), and proteins were subsequently analyzed by western blot ...
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bioRxiv - Plant Biology 2023Quote: ... Concentration of the nuclear and cytosolic proteins were determined with the Pierce™ BCA protein assay kit (ThermoFisher, USA). Pre-cooled buffers and equipment were used for the experiment ...