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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for Rat CCAAT Enhancer Binding Protein Alpha CEBPa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... 13000rpm,10 min) to collect protein and the concentration of protein was determined with the BCA assay (Thermo Fisher Scientific). Western blot was performed following the manufacture’s instruction of the corresponding antibodies (Table S19) ...
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bioRxiv - Microbiology 2022Quote: ... and a small aliquot (30 μL) was removed for protein quantification (Pierce BCA Protein Assay Kit; Thermo Fisher, Waltham, Massachusetts). For lipid quantification ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was used to quantify total protein using the Pierce BCA protein assay kit (Thermo Scientific, cat# 23225) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of EL fractions and TL were determined using the Bicinchoninic acid assay (BCA Protein Assay Kit, Thermo Scientific). Bovine serum albumin was used as a protein standard ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of the cell lysate was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific, USA). The cell pellet was resuspended in 40 μl ddH2O and mixed carefully ...
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bioRxiv - Physiology 2023Quote: ... Proteins were separated by electrophoresis in NuPAGE™ 4 to 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific, USA), and then transferred to Immobilon®-FL PVDF Membrane (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration was calculated by measuring the concentrated proteins with a BCA absorbance assay according to the manufacturer’s instructions (Pierce™ BCA Protein Assay Kit #23225, ThermoFisher).
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was measured by BCA according to the manufacturer’s protocol (Thermo Scientific™ Pierce™ BCA Protein Assay Kit) and 4X LDS sample buffer was added ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of each sample were withdrawn for total protein concentration determination (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2022Quote: Protein concentrations of each sample were determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek ...
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bioRxiv - Plant Biology 2022Quote: ... The total protein concentration for each sample was measured using the Pierce 660 nm Protein Assay kit (Thermo Scientific™). At this point two different experiments were performed ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein content in the seed preparation was measured by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 2% (w/w ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was measured by BCA according to the manufacturer’s protocol (Thermo Scientific™ Pierce™ BCA Protein Assay Kit) and 4X LDS sample buffer was added ...
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bioRxiv - Molecular Biology 2022Quote: ... we performed RNA-Protein pull-down assay by using Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific). The biotin labeled RP24-315D19.10 and AS-RP24-315D19.10 probe was designed and synthesized by GenePharma company ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and the resulting supernatants were collected and assayed for protein concentration with a BCA Protein Assay Kit (Thermo Fisher, 23225). Samples containing equal amounts of protein were denatured by boiling for 10 min with Laemmli sample buffer and then fractionated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined in a 96-well format using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total protein concentrations in CA1 and amygdala homogenates were quantified using Pierce BCA Protein Assay (Thermo Scientific Cat. No. 23225).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total protein concentration of the cleared lysate was assessed with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). IL-1β was quantified from hippocampi tissue lysates with V-PLEX Mouse IL-1β Kit (Meso Scale Discovery ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate was cleared by centrifugation at 11,000 g for 5 min and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein content of the resulting lysate was quantified using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 25 µl of the supernatant was used to determine the protein amount following the manufacturer’s instructions using a Pierce BCA Protein Assay Kit (Cat#23225, Thermo Scientific). A vial containing a quality control mixture pool of 12.5 µl from each sample (150 µl total ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μg protein was loaded onto BoltTM 4-12% Bis-Tris mini protein gels (catalog #NW04125BOX) or NovexTM 14% Tris-Glycine mini protein gels (catalog #XP00145BOX) from Invitrogen. Electrophoresis was run at neutral pH in NuPAGETM MOPS running buffer (catalog #NP0001 ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of the resulting protein pellet was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Cat. 23225) and a NanoDrop™ 2000/2000c spectrophotometer (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: Protein concentrations of each sample were determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample protein concentration was measured using the Micro BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA; Cat.: 23235). 20 μg of total protein lysate was resolved by electrophoresis on Bis-Tris 4-12% NuPAGE polyacrylamide gels using the Novex Mini Cell system (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The protein concentration in the MFS was quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Cat. # 23225), using BSA as a standard ...
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bioRxiv - Immunology 2024Quote: ... the phage:antibody complexes were immunoprecipitated using 40 μL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) (4°C for 4 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... and the cytosolic and membrane proteins were separated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher: 89842) with cOmplete™ Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2024Quote: ... was added to a 255μl H1N1/pdm09 suspension [2mg/ml of viral protein quantified using the modified Lowry protein assay (Thermo Scientific™ Pierce™ Modified Lowry Protein Assay Kit ...
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bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... The plate was gently agitated for 20 minutes at room temperature and the protein concentration was measured using a Pierce BCA Protein Assay Kit (cat. #0023227, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: Total protein concentration in the purified samples was determined with the Pierce Rapid Gold BCA Protein Assay kit (ThermoFisher A53225) by comparing absorbance signal at 480 nm to a BSA standard curve ranging from 0-2000 μg/ml using manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were extracted in cold RIPA lysis buffer and supernatant was used to determine protein concentration using the Pierce BCA protein assay kit (Thermofisher). 50 μg protein was loaded on Mini-PROTEAN TGX Stain-Free Gels before transfer to Nitrocellulose membranes (Biorad) ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Developmental Biology 2022Quote: Proteins from WT C57BL/6J testis were isolated utilizing the T-PER™ Tissue Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with Halt Protease Inhibitor Single-Use Cocktail EDTA-Free (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of the eluted proteins were separated by migration on a NuPAGE 4–12% Bis- Tris Protein Gel (Invitrogen) and silver stained ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 5,000 xg and protein concentration was quantified by Pierce BCA Protein Assay (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected and protein concentration determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Sci., Cat. no: 23225).
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein concentration was quantified using a Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... total proteins from RVLM-containing tissue punches were extracted using N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: The protein concentrations of renal samples were determined with a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, USA). Protein samples (50 μg ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein levels were quantified using a Pierce™ Microplate BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). A total of 30 μg protein was separated by SDS-PAGE at 200 V for 45 min using a 4%–12% precast polyacrylamide gel (Novex ...
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bioRxiv - Biochemistry 2022Quote: ... The For3 was diluted and spun at 400,000 g for 30 min each time before use and the protein concentration in the supernatant was determined by Bradford protein assay (ThermoFisher). The protein gradually loses its activity on ice overnight.
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bioRxiv - Cell Biology 2023Quote: ... The protein concentrations of the both the tissue and cell lysates were assessed by BCA Protein Assay Kit (ThermoFisher #23227) and protein samples were supplemented with 4x laemmli blue (Bio-Rad #1610747 ...
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bioRxiv - Cancer Biology 2023Quote: ... Purity of histones was evaluated by SDS-PAGE and protein concentration was determined using Qubit™ Protein Assay Kit (Invitrogen). Histones were digested using the Propionylation-PIC method (Maile et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Soluble protein content was quantified using the Pierce™ BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Hampton, NA, USA), and the samples were diluted to a final concentration of 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the amount of protein per sample was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat. 23225). Trypsin digestion ...