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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and incubated with anti-KLK10 (BiossUSA bs-2531R, 1:100) primary antibody overnight at 4°C followed by Alexa Fluor-647 (ThermoFisher Scientific, 1:500) secondary antibody for 2hrs at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were preincubated in 3% Bovine serum albumin-PBS at room temperature for 1 h and incubated overnight at 4°C with primary antibodies against CC-ORF (rabbit anti-GFP, polyclonal, Invitrogen A11122, 1:1000). The sections were then incubated with the corresponding fluorescein-conjugated secondary antibodies (Alexa Fluor 488 anti-rabbit ...
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bioRxiv - Developmental Biology 2023Quote: ... -GFAP (1:500, SynapticSystems) -Aquaporin-4 (1:200, Alomone Labs) as well as with Click-iT™ EdU Cell Proliferation Kit (Thermo Fisher Scientific) according to manufactures protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h and incubated overnight at 4°C with the following primary antibodies: anti-GFP (Thermo Fisher Scientific, A-11122, 1:1000), anti-TIAR (BD Transduction ...
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bioRxiv - Cell Biology 2024Quote: ... 3D or in suspension as described above were incubated with cell-impermeable 4 µM ethidium homodimer 1 (EthD-1; Invitrogen catalogue no. E1169) and 10 µg/ml cell-permeable Hoechst (Thermo Fisher catalogue no ...
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bioRxiv - Cell Biology 2024Quote: ... then stained for 1 h at 4°C in PBS with 1:1000 Alexa-647 conjugated M1 mouse anti-FLAG antibody (Invitrogen, Cat #MA1-91878). Cells were moved to flow cytometry (BD FACS Canto2) ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... were injected in the pancreas with 4 ml of collagenase solution (7 ml HBSS, 70 μL HEPES (10 μM) (Gibco), 16,5μL DNAse (10mg/mL ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Biophysics 2019Quote: ... N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine] (Thermo Fisher Scientific, Inc., Waltham, MA). Y-ATRAM (Table 1 ...
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bioRxiv - Physiology 2020Quote: ... and incubated in cell* dishes at 37° C for 4 – 7 hours before adding cell culture medium (RPMI 1640, -glucose +glutamine, Gibco), supplemented with penstrep (10,000 U/ml penicillin / 10,000 μg/ml streptomycin ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Each 500 μl sonicated chromatin (1×106 cells) pre-cleared with 10 μl Protein G Magnetic bead (Invitrogen). 5 μl (1% ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were next incubated with secondary antibodies for 1 hr in RT and mounted with Fluoromount-G (Invitrogen) on slideglasses ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 cm and stained with colloidal Coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). Each lane was cut into 1 band ...
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bioRxiv - Cancer Biology 2020Quote: ... HepG2 cells were grown in Dulbecco’s Modified Eagle Media (DMEM) containing 1 g/L glucose (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... PANC-1 and HEK293T cells were maintained in DMEM with 4.5 g/l D-Glucose and GlutaMAX (Gibco), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with HA antibody (at a conc. of 1:100) pre-bound Protein-G Agarose bead (Invitrogen) or with pre-blocked anti-FLAG M2 beads and rotated overnight at 4□C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown in high glucose (4.5 g L−1) Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in either D-PBS (which contains [1 g/L] D-glucose) (Life Technologies #14287-080) or AA-free DMEM (US Biologicals #D9800-13 ...
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bioRxiv - Cancer Biology 2021Quote: ... horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody diluted 1:2000 (Thermo Fisher Scientific Inc., G-21234). Membranes were washed with PBST (0.1% Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... cells were grown in NB medium with Fe0 granules (2 g;1 to 2 mm diameter; Thermo Scientific) as the sole electron donor ...
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bioRxiv - Cancer Biology 2024Quote: ... All media were supplemented with 10% fetal bovine serum (Hyclone) and 1% penicillin-streptomycin G (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (penicillin G/ streptomycin, Gibco, Waltham, MA, United States) in a humidified atmosphere containing 5% CO2 at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... EGFP-labeled MDA-MB-231 cells were cultured in low glucose DMEM (1 g/ml D-Glucose, Gibco) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... Stanford University) were cultured in low-glucose (1 g/L) DMEM with phenol red (Gibco, USA; powder stock), 1 g/L sodium bicarbonate ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (penicillin G/Streptomycin, Gibco, Waltham, MA, United States) in a humidified atmosphere at 37º C and 5% CO2.
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bioRxiv - Cell Biology 2022Quote: ... and WI-38 VA-13 cells were cultivated in 4.5 g L−1 Dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (Gibco), 2 mM glutamine (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...
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bioRxiv - Immunology 2023Quote: After incubation with the specific HRP-conjugated antibodies anti-rabbit (1:10000, Thermo Fisher Scientific, Cat# G-21234), anti-mouse (1:10000 ...
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bioRxiv - Immunology 2024Quote: ... To enrich for MCL-1 binding protein immunoprecipitation was preformed using the Dynabeads Protein G IP Kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (goat-anti-rabbit, G-21234, 1:2000, Thermo Fisher) in the same TBS buffer for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... the secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) or Alexa Fluor 488 Goat Anti-rabbit IgG (H/L ...
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bioRxiv - Cancer Biology 2024Quote: ... The secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) and Alexa Fluor 488 Goat Anti-mouse IgG (H/L ...
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bioRxiv - Immunology 2024Quote: ... Prior to incubation completion Pierce Protein A/G Agarose Beads (ThermoFisher Scientific, 30 μL per 1 mg protein) were washed with ice-cold PBS (1 mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C (for P. miniata) or 20C (for P. regularis) in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... siRNA (4): s35234 and siRNA (5): s35235 (Silencer Select, ThermoFisher Scientific, USA); EMC2 s18670 and EMC5 s41131 (Silencer Select ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was stained with 5 μg/ml FM 4-64FX (Invitrogen) fluorescent dye while the nucleoid was stained with 2 μg/ml DAPI (Millipore Sigma ...
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bioRxiv - Systems Biology 2019Quote: ... the reaction was quenched with 4 μL 5% hydroxylamine (Thermo Scientific, 90115) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... 2.5-5 µg was loaded onto a NuPAGE 4-12% gel (ThermoFisher) and run at 100V for 2-3 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and passaged every 4-5 days using StemPro Accutase (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... 5 ng/ml IL-4 (Thermo Fisher Scientific, Cat#14-8041-62) and 1 ng/ml TGF-β1 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2023Quote: Samples were loaded with 5 μM Fluo-4 AM (ThermoFisher Scientific, Germany) in CM for 30 min in a cell culture incubator (37 °C and humidified 5% CO2) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... After 4-5 minutes 30 µM SCH-23390 (Fisher Scientific Cat#: 092550) in ACSF was applied for 1 minute ...