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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Rats received intraoperative subcutaneous fluids for hydration (Lactated Ringer Solution, Thermo Fisher Scientific, Waltham, MA, USA) and buprenorphine (0.05mg/kg ...
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bioRxiv - Cell Biology 2023Quote: ... anti-rabbit Alexa 405 and anti-rat Alexa 488 were used at dilutions 1:400 (Invitrogen). Atto 594 NHS-ester was used for bulk proteome labelling (Merck 08741) ...
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bioRxiv - Developmental Biology 2023Quote: ... were coated with 10µg/ml collagen I rat tail diluted in PBS (Cat #A10483-01, Gibco) at 4°C and then at 37°C for 1h each ...
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bioRxiv - Cell Biology 2023Quote: ... or goat anti-rat Alexa Fluor 488 (A-11006, Invitrogen, San Francisco, CA, USA; 1:200). Images were acquired using a Zeiss (Jena ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were then incubated with Alexa-Fluor 647 goat anti-rat IgG secondary antibody (Life Technologies) and stained with DAPI to detect nuclei ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse or naked mole-rat fibroblasts were incubated with 10 µm Celltracker fluorescent probes (C34565, Invitrogen) for 45 minutes at respective growth conditions and then plated together with SCC25-Luc-GFP in ultra-low attachment plates using SCC25-Luc-GFP culture conditions ...
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bioRxiv - Biochemistry 2023Quote: ... eFluor™ 450 rat anti-mouse CD31 clone 390 (ThermoFisher, 48-0311-82; 1:200 dilution), eFluor™ 450 rat anti-mouse CD45 clone 30-F11 (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were Alexa Fluor 568 goat anti-rat IgG(H+L) (Invitrogen Lot:430228), Alexa Fluor 568 goat anti-rat IgG(H+L ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were washed and then incubated with donkey anti-rat IgG (H+L) AF647 (A48272, Invitrogen) and donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... Nunc Maxisorp 96W plates (Thermo, 442404) were coated with rat anti-APOE capture antibody (Invitrogen, 701241) diluted 1:1000 in PPS overnight with gentle shaking ...
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bioRxiv - Immunology 2024Quote: ... The sections were subsequently incubated with chicken anti-rat IgG Alexa Fluor 647 (Invitrogen, #A-21472), donkey anti-rabbit IgG Alexa 488 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:50 followed by secondary rat-absorbed Alexa 568-conjugated donkey anti-mouse antibody (Invitrogen) diluted 1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... or with a goat anti-rat secondary antibody conjugated with Cy5 (dilution 1:500, Invitrogen, #A10525) in blocking solution at room temperature without agitation ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then immediately incubated with secondary antibody (goat anti-rat Alexa 488, 1:200, ThermoFisher) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by staining with rat-absorbed Alexa 568-conjugated donkey anti-mouse antibody (Invitrogen) diluted 1:200 with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME) (All from Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Nuclear factor erythroid 2-related factor 2 (NRF2) (ThermoFisher Scientific, Carlsbad, CA), samples diluted 1:2 ...
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bioRxiv - Immunology 2021Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020) ...
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bioRxiv - Microbiology 2020Quote: ... and 20 U/ml recombinant interleukin-2 (IL-2; Thermo Fisher Scientific) at 37° C ...
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bioRxiv - Neuroscience 2020Quote: ... 50mg/ml streptomycin and 2 mM GlutaMax supplemented with 2% B27 (Gibco) and 5% horse serum (Hyclone) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 2 μL forward primer and 2 μL reverse primer (Invitrogen; Table 1), 14.2 μL water (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cardiomyocytes were loaded with Fura-2 acetoxymethyl ester (Fura-2 AM; Invitrogen) as described previously.28 After Fura-2 loading ...
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bioRxiv - Immunology 2022Quote: ... + 200 IU/mL IL-2 (NCI) + 0.08 mM 2-Mercaptoethanol (Gibco, 21985023) (NK-92 Media ...
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bioRxiv - Immunology 2022Quote: ... 20 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... 2 mM L-glutamine and 53 μM 2-mercaptoethanol (all from Gibco). B cells were isolated using EasySep human naïve B cell Enrichment Kit (Stemcell Cat.# 19254 ...
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bioRxiv - Immunology 2019Quote: ... 2 mM L-glutamine and 53 μM 2-mercaptoethanol (all from Gibco) and activated with 2 μg/mL anti-mouse RP105 clone RP/14 (produced in house or BD Pharmingen Cat.# 562191).
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 2 μM fura-2 AM (Invitrogen, Waltham, MA) for 25 minutes at 35°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2 μl of 2 × Superscript III enzyme blend (Life Technologies, 11754). All the reactions were incubated at 25 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 2 min incubation in 2-propanol (Thermo Fisher Scientific, USA) and equilibration with 25 µl of solvent A (0.1% FA in H2O ...
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bioRxiv - Immunology 2024Quote: ... or anti-MD-2 (2 µg/ml) (Thermo Fisher Scientific, PA5-20058) for 3 h at RT ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell extracts obtained from 2×105 cells with an NE-PER Nuclear and Cytoplasmic Extraction kit (Thermo Fisher Scientific, 78833) were loaded into a 12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...
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bioRxiv - Immunology 2021Quote: Cells exposed to SARS-CoV-2 pseudovirus were lysed and mRNA was isolated with the mRNA Catcher™ PLUS Purification Kit (ThermoFisher). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... rRNA was removed from 20 μg of RNA with 2 reactions of the MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg linearized plasmid DNA was used in a 30 µl reaction with the mMessage mMachine SP6 Transcription Kit (Invitrogen: AM1340). RNA integrity was examined on a 1% agarose gel ...
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bioRxiv - Molecular Biology 2020Quote: ... was performed as follows: 2 μg of messenger RNA (mRNA) was reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Immunology 2022Quote: ... the SARS-CoV-2-TCR mRNA was transcribed in vitro using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Scientitic) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2019Quote: PAT-PCR for the FBF target cyb-2.1 and control tbb-2 was performed using a Poly(A) Tail-Length Assay Kit (Thermo Fisher Scientific). RNA templates from fbf-1(lf) ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the Express one step RT-qPCR Universal kit (ThermoFisher Scientific) using 3.5µL of RNA and 6.5µL of RT-qPCR mix that contains 250nmol of each primer and 75nmol of probe ...
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bioRxiv - Neuroscience 2019Quote: The study of proliferation and differentiation of neural stem cells in the dentate gyrus was performed by incorporation of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Imaging Kit; Molecular Probes) to allow the analysis of proliferation and differentiation ...
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bioRxiv - Molecular Biology 2019Quote: ... LB was added to the sorted cells and they were grown at 37°C for 2 hours prior to plasmid isolation using PureLink HiPure Miniprep Kit (Thermo Fisher).
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bioRxiv - Cell Biology 2019Quote: ... Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific). 25 μL of 5 pM library DNA (1.25*10−4 pmol ...
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bioRxiv - Genomics 2019Quote: ... Extracted RNA (500 ng to 2 µg) were used as templates to make cDNA libraries using a High Capacity RNA-to-cDNA kit (Applied Biosystems). TaqMan gene expression assays were designed using GAPDH (Hs03929097 ...
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bioRxiv - Immunology 2021Quote: ... typically 50-100 pmol sgRNA was delivered into 2*105 cells per electroporation using the Neon 10 µL Kit (Invitrogen, #MPK1096) or 500-1000 pmol of sgRNA delivered into 2*106 cells per electroporation using the Neon 100 µL Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-2 μg of total RNA was used to generate cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Gene expression in mouse cells was quantified using specific primers from Life technologies for mGAPDH (Mm99999915_g1) ...