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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... mRNA was transcribed for 2-2.5 h with mMESSAGE mMACHINE kits (ThermoFisher, T7 for pcDNA3, SP6 for pSP64-poly(A)) following supplier’s protocols including DNAse treatment and purified with RNA Clean & Concentrator-5 (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: Colonies were scraped from the Agar plates and transferred to liquid growth medium (Luria Broth) and grown at 37C for 2 h before plasmid DNA isolation (Maxiprep kit Invitrogen).
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bioRxiv - Evolutionary Biology 2022Quote: The concentration of the gel excised Round 2 Multiplex-Nested-PCR product was quantified by using the Qubit dsDNA HS Assay kit (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 2 µg DNAseI digested RNA were used for reverse transcription using the TaqManTM Reverse Transcription kit (N8080234, Thermo Fisher Scientific) with random hexamer primers ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen with PPIA as the housekeeping gene (for primer sequences see Supplementary Table 2).
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bioRxiv - Cell Biology 2023Quote: Cell proliferation was assessed using the 5-Ethynyl-2-deoxyuridine (EdU) incorporation assay (Click-iT EdU Alexa Fluor 488 Imaging Kit, Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... AIW002-2 was reprogrammed from peripheral blood mononuclear cells of a healthy donor with the Cytotune reprogramming kit (Thermo Fisher). 3450 was also generated from peripheral blood mononuclear cells ...
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesized from 1-2 μg total RNA with the Multiscribe High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814).
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bioRxiv - Immunology 2023Quote: ... 2 μg of total RNA was used to synthesise cDNA using the High-Capacity RNA to cDNA Kit (Applied Biosystems). The primer sequences were generated using the primer BLAST software (Basic Local Alignment Search Tool)
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bioRxiv - Developmental Biology 2023Quote: ... The mRNA from plasmid #1 and #2 was transcribed using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific, AM1340) and purified with the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared according to manufacturer’s instructions from 2 ug total RNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems). qPCR reactions were performed in a 384-well format using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Genomics 2023Quote: ... 2 μg of total RNA reverse transcribed using High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA, Cat No. 4368814) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
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bioRxiv - Plant Biology 2023Quote: RNA in situ hybridization was conducted using Invitrogen ViewRNA ISH Tissue 1-Plex and 2-Plex Assay kits (ThermoFisher Scientific) using the manufacture’s protocol optimized for Medicago root and nodules sections (Kulikova et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μl of extracellular RNA were used with the TaqMan™ Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific, A28007). The miRNAs with the least expression variability between the 48 sequenced samples were identified using geNorm (Vandesompele et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was generated using 2 μg of template RNA and a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368813). cDNA was diluted 1:100 and qPCR was performed using PowerUp SYBY Green Master Mix (Applied Biosytems ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen and used with PPIA as housekeeping gene (for primer sequences see Suppl ...
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bioRxiv - Molecular Biology 2024Quote: ... At least 2 μg of purified RNA was reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCRs were carried out on ABI Prism 7900 HT Fast Real-Time PCR System with Taqman 5′-nuclease probe method and SensiFAST Probe Hi-ROX Mix ...
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bioRxiv - Cancer Biology 2024Quote: ... were mixed with 10 pmol Cas9 protein (Integrated DNA Technologies) in 2 µL of Resuspension Buffer R (Neon Electroporation Kit, Cat# MPK1096, ThermoFisher) and incubated at room temperature for 20 minutes to form the CRISPR RNP complex in vitro ...
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bioRxiv - Biochemistry 2024Quote: ... RNA transcripts of viral and host genes were measured by quantitative PCR using primers listed in Table 2 and Power SYBR Green kit (ThermoFisher), on a QuantStudio7 Pro Real-time PCR System (ThermoFisher).
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (2 µg) was then reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, catalog no. 4374966), according to manufacturer’s instructions.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Single-stranded cDNA was synthesized from 2 µg of total RNA using a High-Capacity cDNA Archive Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentrations were measured by ELISAs according to the manufacturer’s instructions in Nunc MaxiSorp™ flat-bottom plates (Invitrogen) using Uncoated ELISA Kits (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Cell culture supernatants were analyzed in duplicates on Ab40 and Ab42 colorimetric ELISAs per manufacturer’s protocol (KHB3481 and KHB3544, Invitrogen). Ab concentrations were normalized per total cell protein concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... was added to the plate after which a colour reaction was generated by incubation with TMB (3,3’,5,5’-tetramethylbenzidine) colorimetric substrate (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher Scientific). The reaction was stopped with 2 M H2SO4 and the plate read at 405nm with a Tecen Infinite M1000 plate-reader ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plates were washed three times and then developed using 50 μL 1-Step Ultra TMB-ELISA substrate solution (ThermoFisher).
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bioRxiv - Biochemistry 2022Quote: ... followed by three washes in 1% BSA and incubation with TMB-ELISA substrate (Thermo Fisher Scientific, Cat. no. 34028) until the light blue color appeared ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated for 15 min at room temperature with 1-Step Turbo TMB-ELISA Substrate Solution (Thermo Fisher), then terminated with equal volume of 1M phosphoric acid ...
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bioRxiv - Biochemistry 2021Quote: ... Binding was visualized through the addition of 50 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher, #34028), incubated in the above plate shaker for approximately 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... ZIKV-NS1 and MV-H were similarly resuspended in coating buffer (50 mM Na2CO3 [pH 9.6]) at a concentration of 1 μg/ml and then plated in 96-well ELISA MaxiSorp plates (Nunc) at 100 μl per well ...
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bioRxiv - Microbiology 2020Quote: ... was resuspended in a coating buffer (50 mM Na2CO3 [pH 9.6]) at a concentration of 1 μg /ml and then plated in 96-well ELISA MaxiSorp plates (Nunc) at 100 μl in each well ...
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bioRxiv - Neuroscience 2022Quote: Serum samples from dams were analyzed via enzyme linked immunosorbent assay (ELISA) for IL6 and TNFα (Thermo Fisher Scientific) following manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... OSP and CT ELISAs were then blocked with warm 200 µl/well of R10 media (RPMI + L-Glutamate (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... in carbonate buffer (carbonate buffer 15 mM pH 9.6) was coated overnight at 4°C in 96 well ELISA plates (Nunc). Wells were then blocked with 5% dry milk for 2h at room temperature ...
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bioRxiv - Immunology 2019Quote: Serum IL-6 and IL-17A concentrations were assessed by ELISA according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: The concentration of cytokines in the 24h and 48h supernatants from the T57 T cell activation assay was measured by ELISA according to the manufacturer’s instructions (IFNγ: Invitrogen eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Cytokine concentrations were measured by TM ELISAs according to the manufacturer’s instructions in Nunc MaxiSorp flat-bottom plates (Invitrogen) using Uncoated ELISA Kits (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The color reaction was started by adding 100 μL of One-step Ultra TMB ELISA substrate (Thermo Scientific #34028) was added to each well (100 μL) ...
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bioRxiv - Immunology 2021Quote: ... was coated onto each wells of Nunc maxisorp high protein-binding 96 well ELISA plate (Thermo Fisher Scientific Inc.) and incubated overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... slgA concentrations in saliva were measured using the ready-set-go ELISA system for human IgA (Affymetrix: eBioscience, USA) with a sample dilution of 1:7500 at the University Hospital RWTH Aachen (SI ...
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bioRxiv - Bioengineering 2022Quote: ... Protocols provided by the manufacturer were adapted to allow ELISAs to be performed in a 384-well plate (ThermoFisher) to minimize medium (sample ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL/well of 1-step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, cat. # PI34029) was added to the plates and incubated for 5 to 10 min at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plates were washed three times and then developed using 50 μL 1-Step Ultra TMB-ELISA substrate solution (ThermoFisher). A similar ELISA protocol was followed for detecting RNC complexes displaying glycosylated DQNATpd except that the plates were coated with 50 μL of 5-μg/mL mouse mAb FB11 in PBS and were subsequently detected with HRP-conjugated anti-mouse IgG.
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected for cytokine profiling by quantitative ELISA for IFNγ and IL-17A as per manufacturer’s instructions (Invitrogen). Prior to flow cytometric analysis for cytokine production ...
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bioRxiv - Microbiology 2024Quote: ... The absorbent (A450) was measured by the plate reader after HRPO substrate (1-Step Ultra TMB-ELISA, ThermoFisher Scientific) was ...
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bioRxiv - Biochemistry 2023Quote: ... the plate was developed using 50 µl of 1-Step TMB Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well ...