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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 2 ug of total RNAs were then used for cDNA synthesis using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative RT-PCR was performed ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Cancer Biology 2020Quote: ... The extracted RNA was reverse-transcribed into cDNA using the Superscript VILO cDNA synthesis kit (Thermo Fisher Scientific, Figure 2) or SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA ...
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bioRxiv - Cell Biology 2020Quote: ... The marcksl1a and marcksl1b DIG-labeled 1.2 kb long riboprobes were generated from plasmids encoding the full-length cDNAs using MEGAscript SP6 or T7 kits (Invitrogen). Embryos were cleared in 70% glycerol overnight and imaged using Leica M205FA microscope.
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bioRxiv - Cell Biology 2020Quote: ... 5-ethynyl-2-deoxyuridine (EdU) labelling performed with Invitrogen Click-iT EdU Alexa Fluor 594 Imaging Kit (Life Technologies, C10339).
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bioRxiv - Microbiology 2022Quote: ... 0.5 to 2 g of RNA was reverse transcribed with the high-capacity cDNA reverse transcription (RT) kit (Applied Biosystems) using gene-specific primers ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 E sgmRNA standards were generated by in-vitro transcription of the generated SARS-CoV-2 E sgmRNA cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA mini kit (Invitrogen) and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... we performed 5-ethynyl-2′-deoxyuridine (EdU) staining using the Click-iT EdU Alexa Fluor 647 imaging kit (C10340, Invitrogen). Four-color image acquisition of three replicates per condition was performed on an SP8 confocal microscope (Leica) ...
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bioRxiv - Cell Biology 2022Quote: Surface proteins on Caco-2 cells were biotinylated using Pierce Cell Surface protein biotinylation and Isolation Kit (Thermo Scientific, A44390) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 2 μg total RNA in 20 μL reaction mixture using a cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized from 2 µg RNA using the Maxima First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, Massachusetts, USA). All qPCR analyses were performed in technical replicates ...
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bioRxiv - Cell Biology 2021Quote: NRVMs were derived from 1- or 2-day old Sprague-Dawley rates using the Cellutron Neomyt kit (Thermo Fisher Scientific). Cell purity was routinely >90% cardiomyocytes ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen with PPIA ...
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bioRxiv - Molecular Biology 2019Quote: cDNA was generated from 0.5-2 μg of RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) in 20 μL final volume following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... prepared minipreps from 2 ml of O/N cultivation at 37 °C using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), checked for the presence of insert using restriction digest and agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of RNA was reverse transcribed to cDNA using the SuperScript™ VILO™ cDNA Synthesis Kit (ThermoFisher, 11754050) or the First Strand cDNA synthesis kit (Roche Applied Science) ...
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bioRxiv - Cancer Biology 2019Quote: ... Click-iT Alexa Fluor 488 EdU (5-ethynyl-2’-deoxyuridine) imaging kit and Lipofectamine RNAiMAX was purchased from Life Technologies. Foetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of RNA was used for cDNA synthesis using the High Capacity Reverse transcription kit (Thermo Fisher Scientific #4368814). Relative qPCR analysis incorporated one housekeeping gene ...
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bioRxiv - Bioengineering 2021Quote: ... where 2 × 105 HeLa cells were treated per electroporation using the Neon® TransfectionSystem 10 L Kit (Thermo Fisher Scientific) with the recommended electroporation parameters ...
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bioRxiv - Molecular Biology 2021Quote: ... Human globin mRNA was depleted from all samples except from samples #1 and #2 using the GLOBINclear kit (ThermoFisher Scientific). The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL rDNase I and 2 μL DNase I Buffer from DNase Treatment and Removal Reagents Kit (ThermoFisher Scientific AM1906) were added to each RNA sample and then incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA Mini Kit (Invitrogen). Thereafter ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Immunology 2020Quote: ... The detection and measurement of SARS-CoV-2 viral load were performed using COVID-19 RT-PCR diagnostic kit with specific Taqman probes (ThermoFisher) of SARS-CoV-2-specific genes encoded by the RNA-dependent RNA polymerase (RdRp/ORF1ab) ...
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bioRxiv - Genetics 2020Quote: ... and then 2 μg was used in to prepare cDNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). After using a nanodrop to determine the concentration of cDNA in the sample ...
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bioRxiv - Microbiology 2021Quote: ... assay using the OPTI Medical SARS-CoV-2 RT-PCR kit following the manufacturer’s instructions on an ABI 7500 Fast instrument (ThermoFisher Scientific). The OPTI Medical SARS-CoV-2 RT-PCR assay detects two different targets in the gene encoding viral nucleocapsid (N ...
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bioRxiv - Physiology 2020Quote: ... We used 2 µg RNA for reverse transcription with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA, USA). The cDNA was diluted (1:10 ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected pregnant mice intraperitoneally with 100 μL of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Alexa Fluor 647 Imaging Kit, Invitrogen) at 1mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and BJ 1-2 μg of total cell RNA was reverse transcribed into cDNA using SuperScript VILO cDNA Synthesis Kit (11754050, ThermoFisher). A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA (2 µg) was reverse-transcribed to cDNA template using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1621). Q-PCR was analyzed in the Applied Biosystems QuantStudio 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... Approximately 2 μg of RNA from each sample were DNAse treated with the DNA-free™ DNA Removal Kit (Invitrogen) in the presence of RiboLock RNase Inhibitor (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The enrichment phase was performed by using the One-Touch 2 device with the Ion Sequencing 400 Kit according to the manufacturer’s instruction (Life Technologies) (Moitinho et al ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Immunology 2020Quote: ... 2 and 3 cDNA were amplified by real-time PCR using the SYBR Green PCR kit (Thermo Scientific, Braunschweig, Germany). The primer sequences used for the real-time PCR were listed in Table 1.
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bioRxiv - Physiology 2020Quote: ... 2 μg of RNA was used to prepare cDNA with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, 4368813) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: 5-ethynyl-2-deoxyuridine (EdU) pulse labeling was performed with the Click-IT EdU imaging kit (Life Technologies; Cat#:C10340) as described in (Perillo et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured by MTT assay using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide kit (Thermo-Fisher Scientific M6494). For the isobologram assays ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was examined on a 1% TAE agarose gel and DNA concentration was determined using the dsDNA BR Assay Kit on a Qubit 2 fluorometer (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... The purified RNA (2 μg) was used for cDNA synthesis using the RevertAid RT Reverse Transcription Kit (Thermo Scientific, USA) in the presence of 100 μM random hexamers (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 2 μg RNA in 20 μl reactions using High-Capacity Reverse Transcriptase kit (ABI, Thermo Fisher). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg total RNA was converted to cDNA using Superscript First-stand Synthesis kit (Thermo Fisher Scientific, Waltham, MA, #11904018). qPCR was performed using TaqMan gene expression Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of total RNA was used for reverse transcription using the high Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Eluted RNA (30 µl) was treated with 2 U of DNase for 30 min using the TURBO DNA-free kit (Invitrogen). RNA was cleaned up using the RNA Clean & Concentrator-25 kit (Zymo ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched from homozygous TCR-transgenic OT-II (CD45.1/.2) donors using the Dynabeads Untouched™ Mouse CD4 Cells kit (Invitrogen). 1 x 105 OT-II cells were transferred intravenously (i.v. ...