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Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... then cDNA was prepared using 2 μg of RNA and the High capacity RNA to cDNA kit (Applied Biosystems cat#4387406) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Bioengineering 2021Quote: ... injected into a petri dish and incubated with 2 µM calcein AM and 4 µM EthD-1 (Live/Dead Viability/Cytotoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37 ° C ...
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bioRxiv - Microbiology 2022Quote: cDNA synthesis and quantitative PCR (qPCR): 2 µg of RNA was reverse transcribed into cDNA using the Superscript III kit (Thermo Fisher) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was prepared and nascent RNA was purified from 2 µg of total RNA with the Click IT Nascent RNA capture kit from Life Technologies according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... 2 μg of RNA were used to generate cDNA libraries using a High-Capacity cDNA reverse transcription kit (Applied Biosystems, 4368814). RT-qPCR was performed using PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: Preparation of F(ab’)2 fragments from the C03 monoclonal antibody was performed with a Pierce F(ab’)2 preparation kit according to the manufacturer instructions (ThermoFisher Scientific). Briefly ...
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bioRxiv - Genetics 2022Quote: cDNA was generated from 2 control iPSC clones and 4 frameshift iPSC clones with the Superscript III kit (Thermofisher 18080-051) following manufacturer’s recommendations using 1ug of RNA per sample and random hexamer primers ...
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bioRxiv - Plant Biology 2022Quote: ... For the cDNA obtention we used 2 μg of the total RNA extract from each sample and the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using 10 pmol random nonamers (Takara ...
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bioRxiv - Microbiology 2022Quote: RNA from Caco-2 cells was used as the template in reverse transcription reactions using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2.5 µg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes [65] using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using SuperScript IV Reverse Transcriptase Kit (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Physiology 2023Quote: ... 2 µg of total RNA was used to generate the cDNA using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific). The reaction was run in QuantStudio 6 Real-Time PCR Systems using 0.2 μl of cDNA in a 15 μl reaction according to the manufacturer’s instruction in triplicate that measures real-time SYBR green fluorescence ...
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bioRxiv - Microbiology 2022Quote: ... Co-cultures were stained with 0.15 μL FUN-1 and 0.5 μL Calcoflour white in 100 μL 10 mM HEPES buffer pH 7.2 + 2% glucose using a LIVE/DEAD™ Yeast Viability Kit (ThermoFisher, Germany) for 30 min in dark at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Eluted peptides were lyophilized and reconstituted in 300 µL 0.2% TFA for fractionation using the Pierce High pH Reversed-Phase Peptide Fractionation Kit following the instructions provided by Thermo Fisher Scientific for TMT-labelled samples ...
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bioRxiv - Cell Biology 2022Quote: ... 10 guts per samples were lysed in 2 X SDS-loading buffer and protein was quantified using Pierce Detergent compatible Bradford Assay kit (Thermo Scientific). The lysates were separated by SDS-PAGE and transferred to Nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) was synthesized from 2 µg of RNA using the Maxima H Minus First Strand cDNA Synthesis kit (Thermo Scientific). Quantitative PCR was performed using SYBR GreenER™ qPCR SuperMix Universal reagent (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of complementary DNA was performed using 1–2 μg total RNA and the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative real-time PCR reactions were performed using SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed following shear stress application by incubating each sample in a solution containing 2 µM calcein AM and 4 µM ethidium homodimer-1 in PBS (Live/Dead Viability/Cytotoxicity Kit; Thermo Fisher) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: All RT-qPCR were performed using SYBR Green I (2×) from Platinum SYBR Green qPCR SuperMix-UDG kit (Thermo Fisher Scientific). Reactions were performed in triplicates on a 96-well plate with a final reaction volume of 20 μL by using 7900HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was synthesized from 2 μg of DNase-treated RNA using a cDNA synthesis kit (Applied Biosystems, Foster City, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then loaded with CCF4-AM for 2 hours at room temperature using the LiveBLAzer Fret-B/G Loading with CCF4-AM kit from Thermo Fisher. After 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: To measure proliferating phNPCs we measured incorporation of 5-ethynyl-2’-deoxyuridine (EdU) into newly synthesized DNA using a Click-iT EdU Imaging Kit with Alexa Fluor 647 (Invitrogen C10340). At 8 days post transduction ...
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bioRxiv - Developmental Biology 2023Quote: ... HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher, C10418) according to the manufacturer’s recommendations.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 µg RNA samples were reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). Additional file 1 presents the SYBR Green assay primers ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of eluted RNA was treated with 2 U of DNase using the Invitrogen Purelink DNase kit (ThermoFisher, Waltham, USA) and incubated at 37°C for 30 minutes followed by inactivation at 62°C for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Residual genomic DNA was removed from samples containing 2 μg of total RNA using the Turbo DNase-free kit (Invitrogen; Thermofisher Scientific) and about 1.8 μg of total RNA was reverse transcribed using a High Capacity RNA to cDNA kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of purified RNA was converted to cDNA with the Maxima H Minus first-strand cDNA synthesis kit (Thermo Fisher), using the random hexamer primer provided per manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... total RNA from expanded HUDEP-2 edited and wild-type clones was collected using the mirVana RNA isolation kit (ThermoFisher Scientific) or RNeasy Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µg of RNA was reverse transcribed using the Maxima First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Immunology 2024Quote: ... For T cell panels (Supplemental table 2, 4, 5, 6) we fixed cells with the FOXP3 Fixation/Permeabilization Buffer Kit (Thermo Fisher) and conducted intracellular/nuclear stains using the FOXP3 Permeabilization Buffer (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... targeting AaeVLG-2 (AAEL000165) was in vitro transcribed from T7 promoter-flanked PCR products using the MEGAscript RNAi kit (Life Technologies). To obtain the PCR products ...
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bioRxiv - Molecular Biology 2024Quote: Reporter DNA templates were PCR-amplified before transcription for 2 h using the MEGAscript® T7 Transcription Kit protocol (AM1334, Invitrogen) (forward primer ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were mixed with an equal volume of SYB Green loading buffer and operated electrophoresis on 2% agarose gel for quantification and qualification followed by purification with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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bioRxiv - Microbiology 2024Quote: The cDNA of SARS-CoV-2 was generated using the High Capacity cDNA Reverse Transcription Kit (Applied BioSystems, Waltham, MA, USA) from the MN908947.3 synthetic SARS-CoV-2 RNA (Twist Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were co-transfected at a 1:2 heavy to light chain ratio into Expi293F cells using the Expifectamine 293 Expression Kit (Thermo Fisher), and antibodies were purified with protein A agarose (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: Secreted IL-10 was determined by ELISA as per the manufacturer’s protocol (Mouse IL-10, Invitrogen, No: 88-7105-88). The color reaction was measured as OD450 units on a Bio-Rad iMark microplate reader ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: To measure the levels of secreted IL-17a from the Th17 cell culture assay we utilized the mouse IL-17a (homodimer) ELISA (ThermoFisher) according to the kit instructions.
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bioRxiv - Animal Behavior and Cognition 2019Quote: Serine protease inhibition assays were performed at 37°C by using a thermostated Multiskan GO ELISA plate reader (Thermo Scientific). Serine proteases and their corresponding substrates (in brackets ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed 3 times with PBS-T and then 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... This was followed by the plate wash step and an incubation with 1-Step Ultra TMB-ELISA (Thermo Scientific, SG) at 100 μL/well for 3 min ...