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Citations for Thermo Fisher :
4551 - 4600 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Separated peptides were eluted directly from the column and sprayed into an Orbitrap Velos Mass Spectrometer (ThermoFisher Scientific, Hemel Hempstead, UK) using an electrospray capillary voltage of 2.7 kV ...
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bioRxiv - Neuroscience 2024Quote: ... Oligomers of Aβ42 were prepared by diluting the peptide solution in phenol red-free F12 medium (Thermo Fisher, Waltham MA, USA) to a final concentration of 100 μM and incubated for 24 h at 4°C followed by immediate use or storage at -80°C ...
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bioRxiv - Bioengineering 2024Quote: ... A one microgram equivalent of peptide was subjected to LCMS/MS analysis on Orbitrap Q-Exactive Plus mass spectrometer (Thermo Scientific) in conjunction with Dionex UltiMate3000 Ultra High-Performance Liquid Chromatography (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µl of this peptide mix was injected onto an Acclaim PepMap RSLC HPLC column (75 µm × 15 cm, Thermo Scientific) connected to the EASY-nLC 1000 system (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were eluted for 35 minutes onto a 0.075 mm × 500 mm Acclaim™ PepMap™ RSLC resolving column (Thermo Scientific) using a liner gradient ranging from 5-28% of buffer B (80% Acetonitrile/0.1% Formic Acid/19.9% Water ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide concentration was calculated based on the peak area of 100 ng of Pierce HeLa protein digestion standard (Thermo Fisher Scientific). Afterwards the peptide solution was frozen at −70°C before further processing.
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bioRxiv - Cancer Biology 2024Quote: ... About 5 µg digested peptides were aliquoted for global proteomics analysis by stage tip desalting and dried using Speed-Vac (Thermo Scientific). 1 µg peptides were loaded on Evotip (Evosep Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... peptide samples were then dried in a speedvac and resuspended in a solution of 3% acetonitrile and 0.1% TFA and desalted using Pierce Peptide Desalting Spin Columns (Thermo Scientific # 89851). Eluants were dried in a speedvac and resusupended in a solution of 3% acetonitrile and 0.1% formic acid to a final concentration of 0.5 µg/µL ...
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bioRxiv - Biochemistry 2024Quote: ... and were subjected to separation on an XBridge Peptide BEH C18 column (Waters) connected to an Accela high-performance liquid chromatography (HPLC) system (Thermo Scientific). A 50-min gradient of buffer B (90% ACN ...
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bioRxiv - Bioengineering 2024Quote: ... to peptides detected using DECODE was performed as follows: 12.5µL per well of 4 mM peptide solutions were coated on 384 well ELISA plates™ (Thermo Fisher Scientific) by shaking at r.t ...
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bioRxiv - Biochemistry 2024Quote: ... cell lysates were spiked with a known concentration of synthetic stable isotope-labeled peptides (Gerber et al, 2003) (AQUA) obtained from Life Technologies: ATPGHTG[C]LSPG[C]PDQPA(R ...
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bioRxiv - Microbiology 2024Quote: ... Eluting peptides were directly analyzed on a Q-Exactive HF mass spectrometer in data-dependent acquisition (DDA) mode (Thermo Fisher Scientific)15 ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ Quantitative Colorimetric Peptide Assay (23275) and Pierce™ Rapid Gold BCA Protein Assay Kit (A53227) were ordered from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: The resulting peptides were analyzed by LC-MS using Nano LC-MS/MS (Dionex Ultimate 3000 RLSCnano System, Thermo Fisher Scientific) interfaced with Eclipse (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Isobaric-labelled peptides from total proteome and phosphoproteomes were then fractionated into eight fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The peptide samples were loaded onto an Acclaim PepMapTM 100 C18 trap column (100 µm x 2 cm, 100 A; Thermo Scientific) and resolved on a PepMap RSLC C18 analytical column (2µm ...
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bioRxiv - Neuroscience 2024Quote: ... sonicated and the peptide concentration was determined through absorbance at 280 nm measurement using a Nanodrop instrument (ThermoFisher, Nanodrop One C).
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bioRxiv - Cell Biology 2024Quote: ... LC-MS analyses of peptide samples were performed on a hybrid ion trap- Orbitrap mass spectrometer (LTQ-Orbitrap Velos, Thermo Scientific) coupled with nanoflow reversed- phase liquid chromatography (EASY-nLC 1200 ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of plasma exosome peptides (pooled from 10 different plasma exosome samples) were injected onto Dionex Ultimate 3000 LC (Thermo Scientific) coupled to ACQUITY UPLC CSH1.7 mm C18 column (2.1 × 150 mm ...
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bioRxiv - Immunology 2024Quote: ... were immunized subcutaneously at two sites on the back flank area with 200 μg of MOG35–55 (MEVGWYRSPFSRVVHLYRNGK) peptide (Thermo Fisher) emulsified in 200 μl incomplete Freund’s adjuvant (BD bioscience ...
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bioRxiv - Developmental Biology 2024Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Microbiology 2024Quote: Peptides were analysed through Ultimate 3000 RSLC nano-UPLC system connected with an Orbitrap Elite hybrid mass spectrometer (Thermo Fisher Scientific). Roughly 600 ng of peptides were loaded with automated injections onto a trap column (Acclaim PepMapTM 100 ...
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bioRxiv - Biochemistry 2024Quote: ... was used as a reference protein database and peptide spectra matches were performed using the SEQUEST algorithm within Proteome Discoverer v.2.1 (Thermo Fisher Scientific) with a fragment tolerance of 0.6 Da and parent tolerance of 10 ppm ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and incubated 1 hour at RT with secondary antibodies diluted in blocking solution as follows: anti-guinea pig Dylight 488 (Thermo Scientific, SA5-10094) (0.5 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Staining media was prepared in blocking buffer using alpha-Bungarotoxin (BTX) conjugated with Alexa Fluor 555 (ThermoFisher, B35451, 1:200 dilution in PBS). Additionally ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked with 600 μL blocking buffer (33% sonication buffer, 67% 2x hybridization buffer, 500 ng/μL yeast RNA [Thermo Fisher Scientific], 1% BSA) at room temp for 1 hr ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexaflour 594 (Life Technologies, 1:500) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexafluor 594 (Life Technologies, 1:500) for 1 hour at room temperature.
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bioRxiv - Physiology 2020Quote: ... Slides were subsequently exposed to a 1:5000 dilution of the primary antibody in blocking solution (Alexa488-conjugated rabbit anti-GFP; Life Technologies, Waltham, MA) for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody diluted in blocking solution for 1-2h (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies). Finally ...
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bioRxiv - Bioengineering 2020Quote: ... Slides were blocked in serum-based blocking buffer and incubated with primary antibody against MMP12 (MA5-32011, Invitrogen, 1:50, 20 μg/mL). Slides were washed in PBS (3×5 minutes) ...
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bioRxiv - Biophysics 2021Quote: ... After one hour of blocking with 5% (w/v) skim milk in 0.1% (v/v) Tween™-20 (Fisher Scientific, Hampton, NH, USA) and PBS (PBS-T) ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated overnight at room temperature with the indicated primary antibody prepared in blocking/permeabilization solution: mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679) or mouse monoclonal antibody against GLUT-3 (1:100 ...
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Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neuronsbioRxiv - Developmental Biology 2021Quote: ... cells were washed with 0.1% PBST and further incubated with blocking serum containing the corresponding secondary antibodies (Streptavidin Alexa Fluor™ 488, 546 or 647 conjugates; Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in 1 mL the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000, Hoechst 33342 at 1:1000 [Thermofisher] and Phalloidin-AlexaFluor555 at 1:200 [Thermofisher] in blocking buffer). The tissue was washed extensively and then mounted on glass slides using VectaShield (Vector Labs ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then permeabilized using 0.1%Triton X-100 for 10 minutes and blocked using Seablock blocking buffer (37527, Thermo Fisher Scientific, MA, USA) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Hemocytes were washed three times with blocking buffer and counterstained with 20 µM Hoechst 33342 (405, Molecular Probes, ThermoFisher Scientific, Waltham, MA, USA) and then mounted by adding 2 drops of Prolong Gold Antifade Mountant (Molecular Probes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with primary antibodies diluted with blocking buffer overnight at 4°C followed by washes and incubation with Alexa Fluor®-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the secondary antibody (1/400 in blocking buffer, goat anti-rabbit Alexa 594 antibody, Thermo Fisher, A-11037 or donkey anti-mouse Alexa 488 antibody, Thermo Fisher, R37114) was added in blocking buffer for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... a secondary antibody was applied to the sections for 2 hours (AlexaFluor goat anti-mouse 594 diluted 1:400 in blocking buffer; Life Technologies, Carlsbad, CA). Sections were again washed in PBS over 30 minutes prior to the application of mounting media and coverslips.
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bioRxiv - Developmental Biology 2022Quote: ... The samples were washed (4 times 30 min, PBST) and then incubated with the secondary antibody (Alexa fluor 647, 1: 1200 ThermoFisher in blocking solution) overnight at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Then samples were incubated for 30 min in blocking buffer containing 2% (w/v) BSA and then incubated with Alexa Fluor 488 phalloidin (1:400 dilution, Thermo Fisher Scientific, A12379) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were rinsed in 0.02M KPBS and incubated for 2 hr at room temperature in blocking buffer containing goat anti-rabbit Alexa-Fluor conjugated secondary antibodies (Life Technologies, Carlsbad, CA). Sections were coverslipped using ProLong antifade mounting medium (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated for 2 hr at room temperature in blocking buffer containing secondary antibodies against rabbit (raised in goat) conjugated with Alexa-Fluor fluorochromes (Life Technologies, Carlsbad, CA) and coverslipped using ProLong mounting medium (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific, PA1-933) and mouse anti-GFP antibody (1:500 ...
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bioRxiv - Genetics 2019Quote: ... primary antibodies in blocking buffer containing 5% milk followed by the secondary antibodies of goat anti-mouse IgG (H+L) HRP (Life Technologies, 1:10000) or goat anti-rabbit IgG (H+L ...