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Citations for Thermo Fisher :
4451 - 4500 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched to 0.5% trifluoracetic acid and spiked with Pierce Retention Time Calibrant (PRTC) peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Developmental Biology 2024Quote: ... Peptides were extracted from brains from 5 d old male flies and analyzed on an Orbitrap Fusion mass spectrometer (Thermo Scientific) equipped with a PicoView ion source (New Objective ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatography of peptides prior to mass spectral analysis was accomplished using capillary emitter column (PepMap® C18, 3µM, 100Å, 150x0.075mm, Thermo Fisher Scientific). Protein annotations ...
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bioRxiv - Cell Biology 2024Quote: ... Online detection of peptide ions was by electrospray ionisation (ESI) mass spectrometry MS/MS with an Orbitrap Elite MS (Thermo Scientific). Ionisation of LC eluent was performed by interfacing the Sharp Singularity emitters (Fossil Ion Tech ...
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bioRxiv - Cell Biology 2024Quote: ... (Roberta Noberini et al., 2021) Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientific), 25-cm long (inner diameter 75 µm ...
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bioRxiv - Immunology 2024Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Microbiology 2024Quote: The synthetic peptides YNGVFQECCQAEDKGACLLPK (STD) and YNGVFQECCQA EDKacGACLLPK (STD*Kac) were analyzed using an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) equipped with an EASY-nLC 1200 system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: Fractionated crosslinked peptides were loaded onto a Vanquish Neo HPLC coupled to a nano-ESI source of an Orbitrap Eclipse (ThermoFisher Scientific). Samples were injected onto a 300μm x 5mm PepMap Neo Trap Cartridge peptide trap column (C18 ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was performed in 30μl of wash buffer plus 5μl of 1mg/ml 3xFLAG peptide solution (Pierce™ 3x DYKDDDDK, Thermo Scientific, A36805). Samples were incubated on ice for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: 50 µg of the peptides resulting after tryptic digest as described above were subsequently labeled using TMT-10plex reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The tryptic digest was separated through online reversed-phase chromatography using a peptide trap column Acclaim PepMap 100 C18 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Freshly isolated mouse splenocytes or LN cells were stimulated with an overlapping peptide pool of RBDWT (10 μg/mL) in the presence of Brefeldin A (Invitrogen, USA) for 5 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT10 (Thermo Scientific, #90110) or TMTpro 18-plex (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: Peptide digests of purified RSVF and antibodies were analyzed in DIA mode on a Thermo Scientific Lumos mass spectrometer (Thermo Fisher) equipped with a nanoelectrospray ion source and coupled to an Easy-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... internal diameter 100 μm) for desalting and a C18 analytical column (length 10 cm, internal diameter 75 μm) for peptide separation (Thermo Scientific). Peptides were eluted with a three-step 45 min gradient of 0.1% formic acid in ACN at a flow rate of 300 nl/min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2023Quote: ... The digested peptides were cleaned-up using the HiPPR detergent removal resin kit (PN 88305, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Fisher Scientific) fitted with a 100 μm × 2 cm PepMap100 C18 nano trap column and a 75 μm × 25cm ...
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bioRxiv - Cell Biology 2023Quote: The TMT-labelled peptide samples were analysed with an Easy-nLC1200 liquid chromatography system combined with Q Exactive HF mass spectrometer (Thermo Scientific) using a 136 min gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately one µg or less for each SEC fraction was injected and the peptides were separated on an Acclaim PepMap RSLC column (150 mm × 75 µm, 2 µm particle size, ThermoFisher Scientific) at a flow rate of 300 nl/min ...
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bioRxiv - Microbiology 2023Quote: ... Multiplexed TMT samples were separated into four fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). TMT labelled fractions were analysed in an Orbitrap Fusion Lumos using a resolution of 50,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides in each sample were labeled with one of the tags of TMTpro 18-plex labeling kit (A52045, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Both the digested peptides from non-enriched and enriched samples were labeled with 16-plex TMT according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2023Quote: LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: Splenocytes were stimulated with the peptide pools described in the memory phenotypic assay and supplemented with anti-CD28 and anti-CD49d mAbs (ThermoFisher Scientific) both at 1.0 μg/ml or a tissue culture medium with 1% DMSO as a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Immunology 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (Thermo Scientific), and separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Molecular Biology 2023Quote: ... peptides were desalted on StageTips (36) and analysed by nanoflow liquid chromatography on an EASY-nLC 1000 system (Thermo Fisher Scientific) coupled online to a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermofisher Scientific) using a 180 min gradient ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting phosphopeptides and whole cell lysates (flow through) were fractionated using Pierce High pH Reverse-Phase Peptide Fractionation kit (Thermo Fisher). Samples were dried and reconstituted in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2023Quote: The LC-MS/MS analyses of TMT-labelled peptides were performed on an Orbitrap Fusion Lumos Mass Spectrometer (Thermo Fisher Scientific) coupled with a Thermo Scientific Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... and AtILR1 open reading frame sequences deleted of the 25-N-terminal signal peptide-encoding codons were amplified using Phusion Taq Polymerase (Thermo Fisher) prior to cloning into pETM11 plasmid in the BL21 (DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG resin was washed with Trisbuffered saline (TBS) and eluted with 25 μL or 2 mg/mL 3X FLAG peptide (Fisher Scientific). Samples were analyzed by mass spectrometry as described below.
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bioRxiv - Biochemistry 2022Quote: ... The beads were bound to the magnet and the supernatant containing the peptides were extracted and dried in a SpeedVac (ThermoFisher Scientific). Samples were brought up in 50 μL of 100 mM TEAB and labeled with TMT10plex (LOT VG306772 ...
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bioRxiv - Biochemistry 2023Quote: ... Digested peptides were separated on an EASY-Spray column (25 cm × 75 μm ID, PepMap RSLC C18, 2 μm, Thermo Scientific) connected to an EASY-nLC 1000 nUPLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: All peptide samples were spiked in with iRT standard peptides (Biognosys AG, Schlieren, Switzerland) and applied to MS analysis using EasyLC nano HPLC (Thermo-Fisher Scientific), Vanquish Neo HPLC system (Thermo-Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18, 5 μm, 50 cm, 100 A° Easy nano spray column # ES903, Thermo Scientific) at a flow rate of 250 nl/minute flow rate by applying nonlinear gradient of solvent-B (80% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... A column for affinity purification was prepared by binding the peptide to a SulfoLink resin column using the SulfoLink Immobilization Kit (Thermo Fisher). Antibodies were affinity-purified from serum using this column.
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bioRxiv - Cell Biology 2022Quote: ... reversed phase liquid chromatographic separations of peptides were performed on a C18 EASY-spray and C18 trap columns connected to an Ultimate 3000 UPLC system (Thermo Scientific). Mass spectra were acquired on an Q Exactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Nanoscale liquid chromatography (LC) separation of tryptic peptides was performed on a Dionex Ultimate 3000 Rapid Separation LC system (Thermo Fisher). Peptides were loaded onto a 20 μL nanoViper sample loop (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were separated on a C18 Acclaim PepMap nanoLC column (75 μm x 50 cm nanoViper, PN 164942, Thermo Fisher Scientific) equilibrated with 4% solvent B (99.9% acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides were separated by means of nanoscale C18 reverse-phase Liquid Chromatography (LC) using an EASY-nLC II 1200 (Thermo Scientific) system directly coupled to a mass spectrometer (Orbitrap Fusion Lumos ...
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bioRxiv - Biochemistry 2023Quote: ... The malonylated synthetic peptide TVDGPSGKmaLWR* containing a stable isotope-labeled arginine residue (R* with 13C6 and 15N4) was obtained from Thermo Fisher Scientific at >90% chemical purity and >99% isotopic purity ...