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Citations for Thermo Fisher :
4351 - 4400 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37°C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Dried peptide mix was resuspended in 2% TFA and fractionated using Pierce High pH Reversed-Phase Fractionmation kit (84868, ThermoFisher Scientific). Each fraction was vacuum-dried and resuspended in 25 μl 0.1% TFA ...
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bioRxiv - Microbiology 2020Quote: Both WCP and vRIC peptides were analysed using a Dionex Ultimate 3000 RSLC nanoUPLC (Thermo Fisher Scientific Inc, Waltham, MA, USA) system online with an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were trapped on a C18 PepMap100 pre-column (300 μm inner diameter × 5 mm, 100 Å pore size, Thermo Fisher) using solvent A [0.1% (v/v ...
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bioRxiv - Pathology 2021Quote: Peptide samples were analysed by nano-LC-MSMS (Dionex RSLCnano 3000) coupled to a Q-Exactive Orbitrap mass spectrometer (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2019Quote: ... Tryptic peptides were separated by nanoscale C18 reverse phase chromatography coupled on line to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) via a nanoelectrospray ion source (Nanospray Flex Ion Source ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were separated on an EASY-Spray HPLC column (25 cm × 75 µm ID, PepMap RSLC C18, 2 µm, Thermo Scientific) using a gradient of 5 - 35% acetonitrile in 0.1% formic acid and a flow rate of 300 nL/min for 45 minutes delivered by an EASY-nLC 1000 nUPLC (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Electrospray MS was performed on the tryptic peptides using an LTQ XL linear quadrapole ion-trap mass spectrometer equipped with in-line liquid chromatography (ThermoFisher Scientific) using a C8 capillary column (300 x150 mm ...
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bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were loaded onto a C18-reversed phase column (75-μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific), separated and MS data acquired using Xcalibur software ...
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bioRxiv - Biochemistry 2019Quote: ... The application of a 2.0 kV distal voltage electrosprayed the eluting peptides directly into the Thermo Fusion Tribrid mass spectrometer equipped with an EASY-Spray source (Thermo Scientific). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (Thermo Scientific) ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... the rhodopsin was eluted in 2mL elution buffer containing 1.25 mg (265 μM) Pierce™ 3x DYKDDDDK Peptide (Thermo Scientific, USA). The eluate was concentrated using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-10 membrane (Millipore ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Re-dissolved samples were loaded on a ReprosilPur C18 reverse phase column and peptides were analyzed using a nano-HPLC Dionex Ultimate 3000 system (Thermo scientific) coupled to an Orbitrap Velos mass spectrometer (Thermo scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Custom PP2A-Aα antibody was generated by immunizing rabbits with the peptide sequence of MAAADGDDSLY conjugated to matriculture Keyhole Limpet Hemocyanin (Thermo Scientific). Antigens were injected into New Zealand White rabbits using an institutionally approved protocol and animal-care facility (Pocono Rabbit Farm and Laboratory ...
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bioRxiv - Cell Biology 2019Quote: ... antisera were diluted with 0.1 vol 10X PBS and incubated overnight with immunizing peptide covalently coupled to sulfo-link resin (Thermo Fisher Scientific). After washing with PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of the peptide was determined using the BCA method and passed through a vacuum centrifugal concentrator (Thermo Fisher, USA), and the dried peptides are sealed and stored at −80°C ...
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bioRxiv - Neuroscience 2019Quote: ... The reconstituted peptides were separated by two-dimensional nano reverse-phase liquid chromatography (Dionex NCS-3500 UltiMate RSLCnano UPLC) EasySpray NanoSource (Thermo Scientific), ionized using an EasySpray NanoSource (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Immunology 2021Quote: ... RNA-optimized Spike genes were designed based on the SARS-CoV-2 Spike protein sequence from Wuhan-Hu-1 (GenBank: MN_908947) with its original signal peptide and the DNAs were synthesized by GeneArt (Thermo Fisher Scientific). All Spike plasmids have the 2P stabilizing mutations by substituting of K986V987 [34–36] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... About 1.5 micrograms of peptides from each sample were used for DIAMS measurement on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific) platform coupled to a nanoelectrospray ion source ...
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bioRxiv - Systems Biology 2020Quote: ... Single U-937 monocyte and macrophage cells were isolated and distributed into 1μl of pure water with MassPREP peptide mixture (25fmol/μl final concentration, Waters 186002337) in 384-well PCR plates (ThermoFisher AB1384) using a BD FACSAria I cell sorter ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were analyzed by LC-MS/MS using a data-independent acquisition method as described previously.69 Extracted ion chromatograms for fragment ions derived from tryptic peptides were plotted using Xcalibur Qual Browser (Thermo Scientific). The area under the curve for each peak was used for quantification.
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bioRxiv - Physiology 2020Quote: ... 50 μg of peptides from each sample were labelled with 5 μl of Tandem Mass Tag 10-plex (TMT, Thermo Scientific) reagent (0.02mg/μl ...
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bioRxiv - Systems Biology 2020Quote: ... peptides were fractionated at pH 10 with a ‘spider fractionator’ coupled to an EASY-nLC 1000 chromatography system (Thermo Fisher Scientific) as described previously31 ...
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bioRxiv - Biochemistry 2020Quote: ... The liquid was evaporated to dryness and the peptides were separated on an EASY-spray column connected to an EASY-nLC 1000 system (Thermo Scientific). The peptides were eluted in a 60 min gradient (from 5-26% of buffer B (2% acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... were centrifuged at 16000 g for 30 min and the concentration of peptides were measured by Nanodrop one Microvolume UV-Vis Spectrophotometer (Thermo Fisher). All peptide samples were analyzed in technical triplicate by LC-MS using an Easy-nano LC 1200 system coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... the theoretical m/z values for all peptides were used to interrogate the raw data using the Quan Browser software program (Thermo Scientific) (Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... After desalting using C18 Stage tips extracted peptides were separated on an Easy-nLC 1200 system coupled to a Q Exactive HF mass spectrometer (both Thermo Scientific) as described elsewhere by Kliza et al (22 ...
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bioRxiv - Biochemistry 2021Quote: ... The digested elutions samples were reconstituted with 0.1% trifluoroacetic acid (TFA) in LC-MS grade water and loaded onto the Pierce peptide desalting spin columns (Thermo Fisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: Processed peptides were measured by reversed phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an EASY nLC1000 (Thermo Scientific) coupled to a quadrupole orbitrap mass spectrometer (Q-Exactive ...
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bioRxiv - Biochemistry 2020Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (3 µm, 75µm x 25cm, Thermo Fisher Scientific), in a 120 min gradient of 5% to 40% B at 300 nL/min ...
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bioRxiv - Microbiology 2020Quote: ... Twelve micrograms of peptides were labelled with TMT labels (6-plex) according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA) and pooled ...
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bioRxiv - Microbiology 2020Quote: ... The resulting tryptic peptides were dried by speed vacuum at 4 °C and desalted with a C18 spin column (Thermo Scientific) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... Peptide mixtures were loaded onto a PepMap 100 C18 trap column (75 μm id x 2 cm length; Thermo Fisher Scientific) at 3 μL/min for 10 min with 2 % acetonitrile (volume fraction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were trypsin digested and peptide mixtures were analyzed by a Q-Exactive mass spectrometer coupled to an Easy-nLC system (both from Thermo Scientific). All MS/MS data were processed with Proteome Discoverer 2.1 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). Peptides were sampled on a 300 μm × 5 mm PepMap C18 precolumn (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... samples were proteolysed by a modified filter aided sample preparation 57 and eluted peptides were analysed by LC-MSMS on a QExactive HF mass spectrometer (ThermoFisher Scientific) coupled directly to a UItimate 3000 RSLC nano-HPLC (Dionex) ...
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bioRxiv - Immunology 2021Quote: Mass spectrometry data acquisition: Analysis of TMT labeled peptide digests was carried out on an Orbitrap Q Exactive HF-X instrument from Thermo Fisher Scientific (Bremen ...
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bioRxiv - Cell Biology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Genetics 2019Quote: ... Approximately 1 μg of peptides from each digested band was analysed using LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher Scientific), as previously described [66].
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bioRxiv - Genetics 2020Quote: ... The resulting peptides were fractionated using a Dionex Ultimate 3000 nanoHPLC system in line with an LTQ-Orbitrap Velos mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Biophysics 2021Quote: ... SARS-CoV-2 ORF10 protein (full lenth, crude, Mass spectrometry data of crude peptides is included in supplementary information) was purchased from Thermo Scientific, USA.
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bioRxiv - Cancer Biology 2020Quote: ... The second half of the data (runs 5-8) was obtained after peptide separation on 75 μm columns packed with 2.6 μm Accucore beads (Thermo Fisher Scientific). Peptides were injected onto 30-40 cm ...
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bioRxiv - Immunology 2022Quote: ... The concentrated peptides were injected into in a Q Exactive HFX instrument (Thermo-Scientific) connected to an Easy-nLC 1200 instrument (Thermo Scientific). The peptides were analyzed in data-dependent mass-spectrometry (DDA-MS ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min acetonitrile gradient ...
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bioRxiv - Neuroscience 2022Quote: ... Eluted peptides were dried down overnight before resuspension in 5% formic acid ready for injection onto the Orbitrap Exploris (Thermo Fisher) mass spectrometer using data independent acquisition (DIA) ...
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bioRxiv - Biochemistry 2022Quote: ... Mass recalibration for each sample was determined manually by performing a separate search on each raw file for linear peptides in Proteome Discoverer 2.4 (Thermo Fisher Scientific) without spectrum recalibration ...
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bioRxiv - Biophysics 2022Quote: ... faecalis was purified as described previously55 and fluorescently labeled at the stem peptide lysine with Alexa Fluor 488 NHS ester (Life Technologies). For labeling ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried peptides were dissolved in 0.1% TFA and analysed by LC-MS/MS on Q Exactive plus mass spectrometer (Thermo Fisher Scientific). Peptide identification was performed using the Mascot search engine (Casado and Cutillas ...
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bioRxiv - Biophysics 2022Quote: ... The cross-linked peptides were separated on a PepMap RSLC column (250 mm × 75 μm, 2 μm particle size, ThermoFisher Scientific). Chromatographic separation was performed with a 60 min gradient starting at 6% and increasing to 40% B (mobile phase A ...