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Citations for Thermo Fisher :
4401 - 4450 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Peptide samples were analyzed on a Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Biochemistry 2022Quote: ... Synthetic peptides were coupled to rabbit serum albumin (RSA) with the linker Sulfo-m-maleimidobenzoyl-N-hydroxysuccinimide ester (Sulfo-MBS, Thermo Scientific) in a carrier (1):linker (50):peptide (50 ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Biophysics 2022Quote: ... Peptides were directly injected over the course of a 75 min acquisition into a Q-Exactive Plus mass spectrometer (ThermoFisher Scientific). Raw MS data were searched against the Uniprot canonical isoforms of the human proteome using the default settings in MaxQuant (version 1.6.12.0) ...
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bioRxiv - Cancer Biology 2022Quote: The resulting peptides were analyzed using a nanoAcquity liquid chromatography (Waters) coupled with a Q Exactive HF-X (Thermo fisher scientific). Samples were analyzed randomly ...
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bioRxiv - Cell Biology 2022Quote: ... 1,000 ng of peptides from each biological replicate were first injected onto an using an Ultimate3000 high-performance liquid chromatography system (ThermoFisher Scientific) coupled online to an Orbitrap HF-X (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Digested peptides were diluted to 60 μL with water and labeled for 1 hour at room temperature using 16plex TMTpro (Thermo Scientific) or 10plex TMT (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted peptides were introduced into the mass spectrometer (Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer, Thermo Fisher Scientific, USA). The spray voltage was set at 2.2 kV and the heated capillary at 270°C ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides eluted from the capillary column were applied directly onto a Q Exactive Plus mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS ...
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bioRxiv - Immunology 2022Quote: ... The sites of phosphorylation of 32P-labeled peptides were also determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer (Kyoto, Japan) with the peptides coupled to an 80 Sequelon-AA membrane (Applied Biosystems), as described previously (Campbell and Morrice ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were initially trapped on a C18 PepMap100 pre-column (300 µm i.d. x 5 mm, 100 Å, Thermo Fisher) using solvent A (0.1% formic acid in water) ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting peptides were collected on a trap column (2 mm × 2 cm) with POROS 20 R2 material (Thermo Scientific) at 0.5°C ...
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bioRxiv - Microbiology 2022Quote: The raw LC-MS/MS data was analyzed against the Uniprot Gallid alphaherpesvirus 2 database (taxon 10390; 1300 sequences) using the Byonic peptide search algorithm (Protein Metrics) integrated into Proteome Discoverer 2.4 (Thermo Fisher Scientific). Optimal main search settings were initially determined with Byonic Preview (Protein Metrics ...
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bioRxiv - Microbiology 2022Quote: ... The peptides were analyzed by capillary LC-tandem mass spectrometry using an Orbitrap Fusion Lumos tribrid ion trap mass spectrometer (ThermoFisher Scientific). The resulting MS/MS data was processed using Sequest HT search engine within Proteome Discoverer 2.5 SP1 against a Homo Sapiens protein database obtained from Uniprot (78 787 entries ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Molecular Biology 2023Quote: Separation of peptides produced by AspN digestion was performed on a ICS3500 nano-UHPLC system (Thermo Fisher Scientific/Dionex, Germering, DE)) employing a 75 μm x 150 mm Easy spray column packed with C-18 reverse phase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The peptides were analyzed by liquid chromatography (LC)-MS/MS (PROXEON coupled to a QExactive HF mass spectrometer, Thermo Fisher Scientific) with three injections of 5 μl digests ...
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bioRxiv - Neuroscience 2023Quote: ... 25 μg of peptide labeled with TMT reagent (0.4 mg, dissolved in 40 μl anhydrous acetonitrile, Thermo Fisher Scientific, Cat # 90111) and made at a final concentration of 30% (v/v ...
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bioRxiv - Physiology 2023Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Fisher Scientific, Cat. No. 23290). The remainder of the peptides from each IP samples (∼0.5-3.5 µg ...
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bioRxiv - Plant Biology 2023Quote: ... A total of 2 μg of re-suspended peptide was injected per replicate using Easy-nLC 1200 system (LC140; Thermo Scientific) and an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Neuroscience 2023Quote: ... Eluted peptides were lyophilized and reconstituted in 300 µL 0.2% TFA for fractionation using the Pierce High pH Reversed-Phase Peptide Fractionation Kit following the instructions provided by Thermo Fisher Scientific for TMT-labelled samples ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Molecular Biology 2022Quote: Peptides were analysed by reversed-phase liquid chromatography on an EASY-nLC 1000 or 1200 system (Thermo Fisher Scientific, Odense, Denmark) coupled to a Q Exactive plus or HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: The prepared tryptic peptide sample of each subject was injected individually into an Ultimate3000 Nano/Capillary LC System (Thermo Scientific, UK) coupled to a Hybrid quadrupole Q-Tof impact II™ (Bruker Daltonics ...
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bioRxiv - Immunology 2022Quote: EAE was induced in 8-12 week old mice by subcutaneous injection with 100 μL of inoculant containing 100μg myelin oligodendrocyte glycoprotein peptide fragment 35-55 (MOG35-55, Thermo Scientific J66557MCR) in PBS with complete Freund’s adjuvant (CFA ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptides were re-dissolved with 0.1% FA solution for LC-MS analysis (Q-Exactive, Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2022Quote: ... Five microliters of peptide mixtures were analyzed in duplicate on a Q-Exactive Plus hybrid quadripole-orbitrap mass spectrometer (Thermo Fisher) as described in Pérez-Pérez et al ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) with a linear gradient reaching 55% MS solvent B after 85 min ...
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bioRxiv - Molecular Biology 2022Quote: ... TMT labeled peptides were analyzed by nano-ultrahigh performance (UHP)LC–MS/MS (Easy-nLC1200, Orbitrap Fusion LumosTribrid, Thermo Fisher Scientific). Proteome Discoverer 2.2 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, ThermoFisher, US) using a 180-min gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... that were spiked with 18 pmol shTMTpro-labeled AS192 triggers were fractionated using the Pierce high pH reversed phase peptide fractionation kit (Thermo Scientific). A total of eight fractions were generated ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 1 μg of re-suspended peptide was injected per replicate using and Easy-nLC 1200 system (LC140; Thermo Scientific) mounted with an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Plant Biology 2024Quote: ... Desalted peptides (100 μg) from each sample were labeled with one of the TMT-10plex reagents (0.8 mg, Thermo Fisher Scientific) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The two supernatants were combined and subjected to high pH fractionation using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Peptides (100 ng) were separated using Acclaim PepMap C18 columns (2 µm, 100 Å, 75 mm, 15 cm; Thermo Fisher Scientific). The peptides were loaded in buffer A (5% acetonitrile and 0.1% formic acid ...
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bioRxiv - Microbiology 2024Quote: A volume of 6 µl phosphopeptides or 500 ng of peptides were directly loaded onto reversed-phase pre-column (Acclaim PepMap 100, Thermo Scientific) and eluted in backflush mode ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated using an Acclaim PepMap RSLC analytical column (C18, 100 Å, 75 μm × 50 cm, Thermo Fisher Scientific, USA) and Acclaim PepMap trap column (75 μm × 2 cm ...
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bioRxiv - Molecular Biology 2024Quote: Fractionated peptides were resuspended in 0.05% formic acid and separated using an UltiMate 3000 RSLC nano-LC system (Thermo Fisher Scientific) equipped with an analytical column (Waters nanoEase HSS C18 T3 ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Equal amounts of peptide fragments from each experimental group were then labelled utilizing the TMTpro 16plex Isobaric Label Reagent (A44520, Thermo Fisher) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated on a capillary column (0.075 mm × 500 mm, Acclaim Pepmap 100, reverse phase C18, NanoViper, Thermo Fisher Scientific) following a gradient of 2-40% buffer B in 128 min (A = 0.1 % formic acid B = 0.1% formic acid ...
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bioRxiv - Molecular Biology 2024Quote: Peptide samples were analysed in a Data-Independent Acquisition mode (DIA) with an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray ion source and a nano-flow LC system (Easy-nLC 1200 ...
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bioRxiv - Neuroscience 2024Quote: ... the separated peptide was analyzed using DIA (data independent acquisition) mass spectrometry with a Q-Exactive HF-X mass spectrometer (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: The peptides extracted from the SCO or control samples were analyzed using a nanoLC system (Dionex UltiMate 3000, Thermo Fisher Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: LC-MS/MS analysis of peptide samples was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA) coupled to an EASY-nLC 1200 liquid chromatography (LC ...
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bioRxiv - Cell Biology 2024Quote: ... the digest peptides from DMSO and Chk2i conditions were respectively spiked with the synthetic lysine-labelled heavy phospho-peptide of (phos)TLC(Carbamidomethylation)GTPNYIAPEVLSK(+8Da) that was synthesized by Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mg of peptides were separated on a reverse phase C18 column and analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 40 ...
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bioRxiv - Cell Biology 2023Quote: The processing of BioID raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer v2.3 (Thermo Fisher Scientific). Raw mass spectra were filtered ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Cell Biology 2023Quote: The processing of whole-cell protein quantitation raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer (PD v3.0 (Thermo Fisher Scientific) using a SPS MS3 reporter ion-based quantification workflow ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 300 µL of 0.1% trifluoroacetic acid and desalted using Pierce™ Peptide Desalting Spin Columns (ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...