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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... consisting of lysis enhancer and resuspension buffer at a 1:10 ratio (both from the CellsDirect One-Step RT-qPCR kit; Invitrogen). Next ...
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bioRxiv - Plant Biology 2021Quote: ... The integrity of the RNA was verified on 1% agarose gel and quantified on the NanoDrop One spectrophotometer (Thermo Fisher). All samples used showed a ratio reading 1.8-2.0 of absorbance at 260/280 nm and 260/230 nm for high-quality RNA.
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Immunology 2019Quote: ... The gag-protease region was amplified by reverse transcription (RT)-PCR from plasma HIV-1 RNA using Superscript III One-Step Reverse Transcriptase kit (Invitrogen) and the following gag-protease -specific primers ...
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bioRxiv - Microbiology 2021Quote: ... We performed one-step multiplex real-time PCR using TaqPath™ 1-Step Multiplex Master Mix (#A28526, Thermo Fisher Scientific), targeting three different SARS-CoV-2 genes with primer and probe sets specific for spike (S) ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200 ...
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bioRxiv - Immunology 2020Quote: ... Wells were washed three times with 1xDPBS followed by a one hour incubation with horse radish peroxidase-conjugated goat anti-human total Ig at a dilution of 1:6000 (Invitrogen). Wells were then washed four times with 1xDPBS followed by a 5-15 minute incubation with TMB substrate (SeraCare) ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were rinsed in PB and then in a one-step incubation containing both Alexa Fluor 647-conjugated donkey anti-goat secondary antibody (1:500; #A21447, Invitrogen) and Alexa Fluor 488-conjugated Streptavidin (1:1000 ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was rinsed briefly in DI water before being stained for one hour with 1:5000 SYPRO Red (Invitrogen) diluted in 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The absorbance of the working SYPRO Orange solution was 0.295 at 468 nm (1 mm path length in a NanoDrop One, ThermoFisher Scientific). For each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.075% (v/v) β-mercaptoethanol and 1 mM benzamidine] supplemented with one tablet of Pierce protease inhibitor cocktail (ThermoFisher Scientific) and lysed by sonication using a Sonics Vibracell instrument (1 s ON/3 s OFF ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in one or more primary antibody: rabbit anti-GFP (1:2000; Molecular Probes Cat# A-11122, RRID:AB_221569), chicken anti-GFP (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Specificity and integrity of dsRNA were checked on 1% agarose gel and quantified with a NanoDrop One© spectrophotometer (ThermoFisher). Double-stranded RNA targeting the gene encoding green fluorescent protein (GFP ...
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bioRxiv - Molecular Biology 2023Quote: The TaqMan assays were performed using a one-step TaqMan probe kit (TaqManTM RNA-to CTTM 1-Step Kit, Invitrogen). As the TaqMan probe we used 12➔7 Probe FAM–ACCATCAGCCCCCTTTTTATTTCCT-MGBNFQ ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were then stained for one and a half hours with Alexa Fluor 568 donkey anti-goat (1:400; A11057, Invitrogen) and DAPI (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... Transposed fragments were PCR amplified for a number of PCR cycles determined from one-third of the maximum fluorescence measured by quantitative PCR reaction with 1 × SYBR Green (Invitrogen), custom-designed primers [70] ...
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bioRxiv - Molecular Biology 2023Quote: ... one with RNAse A treatment (1 µL at 10 mg/mL, ThermoScientific, #EN0531) and one with DNAse treatment (1µL at 2U/µL, Invitrogen, #AM2238). Nucleic acids were separated using denaturing polyacrylamide gel (TBE-Urea gel ...
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bioRxiv - Cell Biology 2023Quote: ... the kidneys from one mouse were harvested and minced into small pieces (∼1 mm3) in Hanks’ Balanced Salt Solution (HBSS, #2323615, Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were stained for one and a half hours with Alexa Fluor 568 donkey anti-goat (1:400; A11057, Invitrogen) and DAPI (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged for 3 min at 500 g and Accutase was replaced with 500ul assay medium containing 2μM 2’,7’ –dichlorofluorescein diacetate (DCFDA) (Thermo Fisher Scientific, cat.no. D399) and 1:1000 live-dead stain Zombie NIR (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... mRNA concentrations were measured using Nanodrop One/One (Thermo Fisher), and 250 ng RNA of each sample was reverse transcribed to cDNA using the Primescript RT kit (Takara Bio ...
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bioRxiv - Immunology 2022Quote: BMDMs were seeded into 6-well plates (one million cells per well) in DMEM containing 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 4 mM unlabeled glutamine ...
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bioRxiv - Genetics 2020Quote: ... Primer pairs producing a single product band of expected size during PCR amplification were shortlisted for fluorescent dye labelling (forward primers) with one of four fluorescent dyes (6-FAM, VIC, NED, or PET, Invitrogen, South Korea) to perform fragment analysis using Applied Biosystems 3130 Genetic Analyser ...
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bioRxiv - Biochemistry 2020Quote: ... one well of a 6 well plate was transfected with 2 μg of plasmid DNA with Lipofectamine® 2000 (Life Technologies™) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... The cells were seeded onto one well of a Synthemax-treated 6-well plate and in StemFlex media containing 1X RevitaCell (ThermoFisher, Cat # A2644501), 30 mM HDR Enhancer (IDT) ...
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bioRxiv - Microbiology 2020Quote: ... and 40 µg of antibody was bound to 3 mg of protein G Dynabeads (Thermo Fisher Scientific) in 400 µL of PBS buffer containing 0.2% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... coverslips were washed 3 times with PBS and mounted with Fluoromount-G with DAPI (Invitrogen, Lenexa, Kansas) to visualize the cells’ nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... and 500 μg of protein lysate was precleared with 3 μL of Dynabeads protein G (Thermo Fisher) for 1 (HEK 293T lysates ...
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bioRxiv - Bioengineering 2020Quote: ... stained slides were rinsed 3 times with TBS buffer then mounted with Fluoromount-G (Thermo Fisher Scientific). Images of the vagus nerve were obtained with 100X magnification using a Keyence BZ-X810 fluorescence microscope (Keyence ...
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bioRxiv - Biophysics 2021Quote: ... Samples were centrifuged for 3 h at 200,000 g (TST 60.4 swing-out rotor, Thermo Fisher/Sorvall). The gradients were manually fractionated ...
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bioRxiv - Genetics 2020Quote: ... was used to obtain allelic discrimination results for the EYS mutation (rs758109813; NG_023443.2:g.1713111del) using a QuantStudio 3 instrument and following the manufacturer’s recommendations (ThermoFisher). See Table S4 for results.
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bioRxiv - Cell Biology 2021Quote: ... 3 μg psPAX2 and 2μg pCMV-VSV-G using 18 uL each Lipofectamine 3000/P3000 reagent (Invitrogen). After 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 3 mg of protein lysates were mixed with protein A/G-magnetic beads (Thermo Fisher 88802) and 2 µg of either anti-Myc or control IgG antibody and rotated for 16 hours at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156, 5 μg/mL puromycin, Life Technologies A11138-03, and 2 μg/mL doxycycline). On day three ...
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bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
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bioRxiv - Physiology 2021Quote: ... The reactions were then incubated for 1h at 4°C with protein A/G dynabeads (Invitrogen) pre-blocked in PBS/0.5% BSA/0.5% Tween ...
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bioRxiv - Immunology 2020Quote: ... During centrifugation at 16500 × g for 10 min at 4 °C 500 μl streptavidin beads (Invitrogen) or each sample was equilibrated in a 1:1 mixture of lysis buffer and 50 mM Tris-HCL pH 7,4 ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and incubated with 40ul washed Dynabeads Protein G (Invitrogen 10003D) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated protein A and biotinylated protein G (4 μg/mL each) (Thermo Fisher Scientific, Illkirch, France), and streptavidin-R-phycoerythrin (4 μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... for 4 hours and afterward incubated with 50 μl Protein G magnetic beads (Invitrogen Cat:#10004D). Beads were washed in RIPA buffer (50Mm Tris-HCl pH8 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants were first precleared for 2 h at 4°C using Protein G Dynabeads (Invitrogen). Precleared samples were immunoprecipitated with anti-H3K9me3 (abcam ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then incubated for ∼4 hours with magnetic A/G beads (Thermo Scientific 78609) again rotating at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysate was incubated at 4°C for 2 hours with Dynabeads Protein G (Invitrogen, 10003D) magnetic beads that bound anti-RNA polymerase II C-terminal domain antibody CTD4H8 (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... day 1 (24 hours) and day 3 (72 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific, Eugene, OR, USA) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... slides were then incubated simultaneously with two different secondary antibodies diluted in PBS/0.1% Tween 20/3% BSA for 1 h at RT to amplify the signal (goat anti-mouse Alexa Fluor 488 (Thermo Fisher, A11001; 1:250) and donkey anti-goat Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... FAZ was visualized via anti-FAZ1 antibody L3B2 (antigenic to Tb927.4.3740, 1:3) (60) followed by secondary Alexa Fluor 488 goat α-mouse IgG antibody (Invitrogen / Thermo Fisher Scientific – 1:1,000).
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bioRxiv - Cancer Biology 2024Quote: ... diluted to a final concentration of 1,500 µg ml−1 and 3 µl of 100 mg ml−1 collagenase IV (Thermo Fisher Scientific, cat. no. NC9836075) diluted to a final concentration of 100 µg ml−1.
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bioRxiv - Cell Biology 2022Quote: ... stained with 8 µl of 5 mg/ml Alexa fluor 488-conjugated phalloidin (Thermo Fisher Scientific, A12379), and incubated in a rotary platform overnight at 4°C in the dark ...