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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Mammalian expression plasmids encoding amber-containing VCP with tandem C-terminal 12X-His and 3X-FLAG tags were generated by Gibson assembly (53) into the pCDNA3.1 vector (ThermoFisher cat. No. V80020). Plasmid for expressing wild-type human VCP in bacteria was obtained from T.-F ...
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bioRxiv - Molecular Biology 2024Quote: ... it was washed thrice with TBS-T and incubated overnight with a primary 6X-His Tag monoclonal antibody (3D5) HRP (Thermo Fisher Scientific) at a concentration of 1 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... Homo sapiens TLR8 extracellular domain fused to a C-terminal thrombin-cleavage sequence followed by a protein A tag was constructed into the pMT-BiP-V5-His vector (Thermo Fisher Scientific). For TLR7 ...
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bioRxiv - Microbiology 2023Quote: ... and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631, 1:500 in TBST). Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-afadin (clone D1Y3Z) or Alexa Fluor 488 anti-DYKDDDDK (flag) tag monoclonal antibody (L5, Life Technologies). The plasma membrane was stained with CF594 dye conjugated wheat germ agglutinin (Biotium) ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the short transcriptional variant of human DA D2 receptor with an N-terminal FLAG tag (SF-D2R-S) was inserted into mammalian expression vector pcDNA 3.1(+) (Invitrogen)62 ...
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bioRxiv - Biophysics 2022Quote: The human wild-type S1PR5 gene (UniProt ID Q9H228) with an N-terminal 3 × HA epitope (YPYDVPDYA) tag was cloned into pcDNA3.1+ (Invitrogen) at KpnI(5’ ...
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Microbiology 2020Quote: ... in vitro transcription of the ligated cDNA (along with a N-gene containing cDNA) using an mMachine T7 transcription kit (Ambion), and subsequent electroporation into BHK-R cells ...
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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bioRxiv - Plant Biology 2022Quote: ... ORFs were cloned using Gateway™ LR Clonase (Invitrogen) into previously described binary vectors containing Omega leader sequences ...
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bioRxiv - Immunology 2024Quote: ... antibodies specific for mouse B220 (clone RA3-6B2) and CD3 (Clone 500.A2, Thermo Fisher) were used ...
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bioRxiv - Cell Biology 2020Quote: ... the STIM1 cDNA was cloned into the pcDNA3.1/V5-His TOPO TA vector (Invitrogen, Cergy-Pontoise, France). All STIM1 variants were generated from the wild-type (WT ...
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Neuroscience 2024Quote: ... The specific miRNA-based shRNA against the mouse open reading frame (ORF) for MSK1 were generated using the BLOCK-iT™ RNAi Designer software (Invitrogen) and cloned into the pcDNA6.2-GW/miR vector according to instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Spike protein (C9-tag) on the surface of pseudovirus was detected using a mouse anti-C9-tag monoclonal antibody 1D4 (Invitrogen, Cat. No. MA1-722), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... entry clones containing individual TSWV proteins were recombined with a pIB/V5-His destination vector (Thermo Fisher Scientific) containing either GFP or mRFP in both amino and carboxy terminal fusions when possible ...
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bioRxiv - Immunology 2020Quote: ... functional grade anti-mouse IL-4 (eBioscience™, clone: 11B11), functional grade anti-mouse INF-γ (eBioscience™, clone: XMG1.2) were from ThermoFisher, Loughborough ...
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Synthetic Biology 2024Quote: ... Green fluorescent protein (GFP) standard curve was created from dilutions of Aequorea Victoria GFP His-tag recombinant protein (ThermoFisher Scientific, Cat. No. A42613) and measured on the BioTek Cytation5 Imaging Reader ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid encoding the N-terminally His-tagged Msm HelD protein was prepared by the GeneArt® Plasmid Construction Service (Thermofisher). Gene construct for HelD expression was designed by codon-optimized back translation of gene MSMEG_2174 from Msm (strain ATCC 700084 / mc2 155 ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti GFP (Thermo Scientific, A-11120, clone 3E6) 1:750 ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-p53 (clone PAb 122, ThermoFisher, MA5-12453), mouse anti-CHK2 (clone 1C12 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-GFP (Life Technologies, #A11120, clone 3E6 IgG2a), rabbit anti-GFP (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... PE-conjugated mouse anti-HLA-DR (clone LN3, Invitrogen); PE-Cy7-conjugated mouse anti-CD11b (clone ICRF44 ...
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bioRxiv - Immunology 2020Quote: ... anti-mouse FcεRI (APC-conjugated, clone MAR-1, Invitrogen), anti-mouse Siglec-F (PE conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... monoclonal mouse anti-Reep2 (clone S326D-2, Thermo Scientific); polyclonal rabbit anti-Rtn4a/b (Abcam #ab47085) ...
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bioRxiv - Molecular Biology 2022Quote: ... Anti-GAPDH clone GAR1 (mouse, monoclonal, ThermoFisher #MA5-15738) was used for western-blotting at 1/2,500 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-HuC/D (Clone 16A11, ThermoFisher A-21271). Secondary antibodies coupled to Alexa488 ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse monoclonal (clone 5D11, Thermofisher #MA1-089, 1:100); anti-V5 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-paxillin (mouse clone 5H11 AHO0492, Thermo Fisher Scientific), anti-phospho-myosin light chain II 3671 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... Golgin 97 (clone CDF4, mouse monoclonal, Thermo Scientific Fisher). The antibody against cathepsin D (CTSD ...
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bioRxiv - Immunology 2024Quote: ... and anti-mouse ICOS (Thermo Fisher, clone 7E.17G9). All flow cytometry was performed with CytoFLEX (Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse GL-7+ (Thermo Fisher, clone GL-7) anti-mouse IgD-(Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... and anti-mouse CD3-(Thermo Fisher, clone 145-2C11). OVA-specific GC B cells were investigated by staining with 1µg/ml Invitrogen™ Ovalbumin ...
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bioRxiv - Developmental Biology 2024Quote: ... a mouse monoclonal antibody (Clone MoBU-1, Invitrogen B35132), which has been verified to be specific to BrdU ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Molecular Biology 2020Quote: ... The V5 epitope tag was detected by using mouse monoclonal anti-V5 antibody (Invitrogen) at 1 ug/ml ...
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bioRxiv - Immunology 2024Quote: ... anti-HA epitope tag (#901513) and Alexa Fluor 647 anti-mouse IgG (Invitrogen #A21235).
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bioRxiv - Biochemistry 2023Quote: ... or 1:6000 for a mouse monoclonal antibody against V5 tag (R960-25; Invitrogen) to detect V5tagged ANGPTL4 ...
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bioRxiv - Immunology 2023Quote: ... 1:5000 and mouse anti-His6 tag polyclonal antibodies (Thermo Fisher, cat# 37-2900) 1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-N-cadherin (1:200, 33-3900, Thermo Fisher Scientific), rabbit anti-E-cadherin (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... Cathepsin L Monoclonal Mouse IgG1 (cat n° BMS166, Thermo Fisher Scientific), anti-clathrin (cat ...
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bioRxiv - Physiology 2024Quote: ... and N-cadherin (1:100 dilution, anti-mouse, #33-3900, Invitrogen) antibodies in blocking buffer at 4°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... AtSUMO1 was cloned into destination vector pK7WGR2 (N-terminal mRFP tag, (Karimi et al., 2002)) by LR reactions using Gateway technology (Invitrogen). Vector specific primer p35s-F used in plasmid construction are listed in Table S2.
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bioRxiv - Biophysics 2020Quote: A full-length cDNA encoding the methyltransferase SUV39H1 was amplified by PCR and fused with an N-terminal His6-MBP tag in pET-Duet1 (Invitrogen).