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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Developmental Biology 2022Quote: ... pMT-yki-HA was generated by cloning Yki-RG and HA tag into pMT-V5-His vector (Invitrogen, Cat# V412020). The Y281A ...
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bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
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bioRxiv - Bioengineering 2024Quote: ... Antibodies were purified by gravity column using Protein G Sepharose 4 Fast Flow Resin (Cytiva) and proteins containing His tags were purified on Ni-NTA Agarose (R90101 Invitrogen).
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bioRxiv - Immunology 2024Quote: Sera competition ELISA assay plates were coated with PBS containing 250 ng of anti-6x His tag monoclonal antibody (Invitrogen) and left covered overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...
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bioRxiv - Microbiology 2024Quote: ... VisAG5 was preincubated 1 hour in the dark at 0.1 mg/mL with 1/10 of an anti-His Tag monoclonal antibody coupled with Alexa FluorTM 488 (MA1-135-A488, Invitrogen). 50 µL of bacteria at OD600 1 was incubated for 2 hours with 5 µL of the fluorescent VisAG5 and subsequently mounted on a slide ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted reaction was then transferred to a tube containing 80 µg of pre-washed Dynabeads His-Tag Isolation and Pulldown magnetic beads (Invitrogen) and incubated on a roller for 5 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Biochemistry 2023Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Microbiology 2024Quote: ... we purified the recombinant Rpf protein with the N-terminus polyhistidine-tag via Ni-NTA Purification (Invitrogen) using a 10 mL gravity-fed column with a 2 mL resin bed ...
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bioRxiv - Microbiology 2020Quote: ... or mouse anti-Muc5AC (clone 45M1, Invitrogen). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... CD8 (primary mouse IgG2b, clone 4B11, Invitrogen), CD68 (primary mouse IgG1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mouse clone BA3R against β-ACTIN (ThermoFisher), rabbit polyclonal against Cytoskeleton-associated protein 4 (P63 ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse CXCR5+ (Thermo Fisher, clone 2G8) and anti-mouse PD-1+ (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse IgE+ (Thermo Fisher, clone 23G3), anti-mouse CD45lo (Thermo Fisher ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... mouse anti-CD81 clone M38 (Invitrogen, #10630D), rabbit anti-HIST1H1.0 clone EPR6537 (#ab125027) ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: mouse anti-His (MA1-21315, Thermo Scientific), goat anti-mouse HRP (405306 ...
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bioRxiv - Immunology 2021Quote: ... capped genome synthesis from linearized cDNA clones (mMessage Machine, Thermofisher) and electroporation of 10-20 μg of RNA into BHK 21 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-N-cadherin Ab was purchased from Invitrogen, mouse anti-actin Ab (1:250 ...
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bioRxiv - Physiology 2022Quote: ... and N-Cadherin (Invitrogen 33-3900, 1:400, mouse) antibodies as described in19 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse monoclonal N-cadherin (3B9) (RRID: AB_2313779, 333900, ThermoFisher). The following day ...
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bioRxiv - Microbiology 2021Quote: ... ACE2-S-tag expression was detected by using a mouse anti-S-tag monoclonal antibody 6.2 (Invitrogen, Cat. No. MA1-981), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... V5 was detected with mouse anti-V5 tag monoclonal antibody (Invitrogen R960), SAG2A was detected using rabbit polyclonal anti-SAG2A antibodies (46) ...
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bioRxiv - Cell Biology 2020Quote: Mouse monoclonal anti-V5 epitope tag was purchased from Invitrogen (R960-25). Rabbit anti-LC3B (ab48394 ...
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bioRxiv - Microbiology 2021Quote: ... V5 was detected with mouse anti-V5 tag monoclonal antibody (Invitrogen R960), human CC2D1A was detected with rabbit anti-CC2D1A antibodies (Sigma HPA005436) ...
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bioRxiv - Microbiology 2022Quote: ... V5 was detected with a mouse anti-V5 tag monoclonal antibody (Invitrogen), MOSPD2 was detected with a rabbit polyclonal antibody (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse anti-V5 tag antibody (#46-0705 [1:1000]; Thermo Fisher Scientific), and horseradish peroxidase-conjugated anti-rat (NA935V [1:1000] ...
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Microbiology 2024Quote: ... and SARS-CoV-2 nucleocapsid protein (N, clone E16C, Fisher Scientific, 1:25) were diluted in blocking solution and then added and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Microbiology 2021Quote: ... Serum supernatant was collected as described above and incubated with a pellet of 900 μl Dynabeads™ His-Tag Isolation & Pulldown (Invitrogen) equilibrated in wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher). The resulting enriched library pool was subsequently sorted using a Sony SH800 Cell Sorter ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Immunology 2024Quote: Mouse PfTrxECL antisera was absorbed against TbpB-LCLEmpty using Dynabeads™ (CAT# 10103D, 10104D) according to the His-Tag Isolation & Pulldown protocol from Invitrogen. ECL-specific Abs were then assessed using Clear Flat-Bottom Immuno Nonsterile 96-well plates (ThermoFisher Scientific ...