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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PE-conjugated rat anti-mouse GATA3 (clone TWAJ; Thermo Fisher Scientific), or FITC-conjugated rat anti-mouse Foxp3 (FJK-16s ...
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bioRxiv - Immunology 2024Quote: ... and mouse IgG1-PE-Cy7 isotype control (clone P3.6.2.8.1, eBioscience/Invitrogen). The cells were stained for 1hr at RT and washed 3 times prior to analysis ...
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bioRxiv - Immunology 2024Quote: ... CD68 (mouse IgG1, clone KP-1; Thermo Fisher Scientific, Waltham, MA), CD163 (mouse IgG1 ...
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bioRxiv - Biochemistry 2024Quote: ... Mouse total Ub antibody (clone P4D1; Thermo Fisher #14-6078-37) was subsequently added as detecting antibody at a final concentration of 5 µg/ml in blocking buffer in 30 μl total volume per well ...
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bioRxiv - Immunology 2024Quote: ... Basophils were defined as anti-mouse CD49b+ (Thermo Fisher, clone DX5), anti-mouse IgE+ (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... and PE-Cy7 mouse IgG1 κ isotype (clone P3.6.2.8.1, ThermoFisher Scientific). The flow cytometer was FACS Verse system (BD Biosciences) ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti-HuC/D (clone 16A11 – 1:50) from Life Technologies. Secondary antibodies coupled to Alexa Fluor 488 ...
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bioRxiv - Biochemistry 2021Quote: ... M-OPG2 and S-OPG2 were generated by inserting the respective cDNAs in frame between NheI and AflII sites of a pcDNA5/FRT/V5-His vector (Invitrogen) containing a C-terminal OPG2 tag (MNGTEGPNFYVPFSNKTG) ...
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GRASP55 Safeguards Proper Lysosome Function by Controlling Sorting of Lysosomal Enzymes at the GolgibioRxiv - Cell Biology 2024Quote: ... was amplified from WI-26 cDNA and cloned into the BamHI/NotI restriction sites of the pcDNA4/TO/Myc-His-A plasmid (V103020, Invitrogen). The RUSH construct for PSAP (pIRES-Str-KDEL-hPSAP-SBP ...
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bioRxiv - Neuroscience 2020Quote: ... A cDNA library was obtained from E15.5 mouse brain using a cDNA synthesis kit (K1681, Thermo Scientific). Then the corresponding cDNA of Pou3f1 was produced with this cDNA library ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting PCR products were utilized to clone pET-26b-Nanobody-ALFA-His in One Shot ccdB Survival 2 T1R Competent Cells (Invitrogen, A10460).
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The LCRN coding sequence was inserted in frame with a N-terminal hexahistidine (His6)-SUMO tag in a Champion pET SUMO expression vector (Invitrogen, Carlsbad, CA, USA) by Gibson Assembly (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into the pFastBac1 vector with a 10xHis tag appended at the N terminus and was expressed in Sf9 cells (Gibco, catalog number 12659017). The cells were cultured at 27 °C for 60 h after being infected with baculoviruses using the Bac-to-Bac system (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
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bioRxiv - Molecular Biology 2024Quote: Human NRL constructs in pcDNA™4/HisMax vector containing an N-terminal Xpress tag (Cat. No. V86420, ThermoFisher Scientific, Waltham, MA, USA) or in pGEX-4 T2 plasmid containing an N-terminal GST tag (GE Healthcare/Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... hKCNK18 (human potassium channel subfamily K member 18) with an N-terminal StrepII-eGFP tag was expressed in FreeStyleTM 293-F (HEK293F) cells (Thermo Fisher Scientific, TFS) and enhanced with 10 mM NaBu 10 h post transduction and harvested 48 h post-transduction ...
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bioRxiv - Developmental Biology 2021Quote: ... kcnk5b cDNA was isolated by RT-PCR from regenerating adult fin cDNA library and cloned into MCS region of pcDNA6-myc-6xHIS-tag plasmid (Invitrogen) or pBluescript harboring the hsp70 zebrafish promoter and GFP coding sequence surrounded by 2 miniTol2 sites ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR was conducted with equal amounts of resultant cDNAs and indicated primers (Table S1) using Platinum Tag DNA Polymerase High Fidelity (Invitrogen) under thermal cycling for 2 min at 94°C followed by 25 cycles of 20 s at 94°C ...
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bioRxiv - Cancer Biology 2024Quote: ... A BAC clone spanning the mouse Myc super-enhancer region (Clone ID: RP23-203H19) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... or HA-Tag (Invitrogen) antibody for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... anti-SNAP tag (Invitrogen), and DAPI ...
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bioRxiv - Cell Biology 2024Quote: ... anti-V5-tag (Invitrogen), HRP-conjugated anti-Rabbit (Abcam) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-V5-tag (Invitrogen), anti-GID8 (Proteintech) ...
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bioRxiv - Systems Biology 2024Quote: ... TMT10plex tags (Thermo Scientific) were dissolved in anhydrous ACN and added to the peptide sample in a 1:10 peptide:TMT ratio ...
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bioRxiv - Immunology 2020Quote: ... Isotype control and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were purchased from Thermo scientific. SP600125 and C-16 purchased from Calbiochem ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike ORF was synthesised (GeneArt; Thermo Fisher Scientific) with a poxvirus early transcriptional stop sequence (T5NT ...
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bioRxiv - Molecular Biology 2022Quote: ... ORFs were then recombined into a custom pEF DEST51 destination vector32 (ThermoFisher) engineered to direct expression of the ORFs as fusion proteins with a V5 epitope tag and MCP appended C terminally and under the control of the EF1-alpha promoter to create ORF–V5–MS2BP constructs ...
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bioRxiv - Biochemistry 2024Quote: ... VPS35L ORF was changed to a codon-optimized sequence from Thermo Fisher. Typical yield was ∼1 mg of purified Retriever from 3 liters of Sf9 culture ...
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bioRxiv - Genomics 2023Quote: ... The ORFs were cloned into the pcDNA3.1(-) backbone (Thermo Fisher Scientific V79520) between the EcoR1 and BamH1 sites ...
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bioRxiv - Immunology 2023Quote: ... The DUSP1 gene ORF (Ensembl, ENSG00000120129) was synthesized from GeneArt (Thermo Fisher) and was cloned into pT2/HB vector through In-Fusion HD Cloning Plus kit (Takara Bio ...
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bioRxiv - Immunology 2024Quote: ... The ORFs were moved into pTRIP.CMV.IVSb.ires.TagRFP-DEST using LR Clonase II (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and RtoK ORFs were individually PCR amplified and cloned into pDONR221 (Invitrogen) via a Gateway BP Clonase II Enzyme Mix (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Sense target cDNA was prepared using the Affymetrix GeneChip WT Plus kit (Applied Biosystems P/N 902281). It was then fragmented and biotin-labeled using the GeneChip WT Terminal Labeling kit (Applied Biosystems P/N 900670 ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Developmental Biology 2024Quote: ... stained with primary antibodies against GFP Tag Monoclonal Antibody (3E6) (1:500, mouse, Thermo Fisher Scientific, A11120) and cleaved Caspase-3 (rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of protein lysates were incubated with 5 μg of His-RILP (NovoPro Labs #512098) or His-Myc using His Pull-down kit (Thermo Scientific #21277) for 2 hours at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... N,N,N’,N’- tetramethyl ethylenediamine and glycylglycine were purchased from Acros Organics (New Jersey, USA). Protogel 30% acrylamide ...
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bioRxiv - Microbiology 2021Quote: ... 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM, Invitrogen), calcium Ionophore A23187 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: Entry clones were prepared using hC9ORF78 cDNA (DNAsu) and pENTR directional TOPO cloning kit (Invitrogen). The entry clone was confirmed by sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... followed by C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For SARS-CoV-2 RBD VOCs proteins ...
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bioRxiv - Immunology 2022Quote: ... followed by a foldon trimerization motif and C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For competition ELISA experiments ...
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bioRxiv - Bioengineering 2021Quote: ... N,N′-Diisopropylcarbodiimide (DIC, Fisher Scientific) and 10 equivalents of DIPEA in DMF for 4 h ...
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bioRxiv - Bioengineering 2024Quote: ... N,N-dimethylethanolamine (Fisher Scientific Company), 2-methoxyethanol (Thermo Scientific Chemicals) ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... from a cDNA library and cloned into pcDNA™3.1/His expression vectors (Catalog #V38520, Life Technologies Co., Grand Island, NY). Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170 ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-β-catenin (Abcam, 1:1000 mouse anti-N-cadherin (Invitrogen; 1:500), mouse anti-Glutamine Synthetase (GS ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated o/n with either 1:1000 mouse anti-GFP (Invitrogen, Ref: A22230) or 1:1000 rabbit anti-RFP (Rockland ...
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bioRxiv - Neuroscience 2020Quote: ... Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen). For luciferase assays ...
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bioRxiv - Neuroscience 2020Quote: ... Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen). For luciferase assays ...