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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Parvalbumin (1:2000; Thermo Fisher; Cat# PA1-933), Parvalbumin (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... Parvalbumin (1:2000; Thermo Fisher; Cat# PA1-933), Parvalbumin (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... The ORF thus PCR-amplified was cloned into the pcDNA™3.1/V5-His TOPO™ TA vector (ThermoFisher Scientific, Cat # K480040) following manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Microbiology 2024Quote: ... plates were coated overnight at 4°C with anti-His tag mAb (Thermofisher, Cat. #PA1-983B) then washed three times with 0.2% (v/v ...
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bioRxiv - Immunology 2024Quote: ... and incubated with anti-His tag antibodies (#MA1-21315, Invitrogen; diluted 1:5,000 in PBS-T) or murine anti-CPAF immune sera (pooled from mice immunized with CPAF protein ...
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bioRxiv - Microbiology 2022Quote: ... or the mouse-anti-His (HIS.H8) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-His antibody (Invitrogen cat. # MA1-21315-1MG) was used at the same concentration to coat the plates ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Genetics 2020Quote: ... The human PROS1 cDNA (GenBank NM_000313.4) was PCR-amplified from HepG2 cDNA and cloned into pcDNA3.1/myc-His(−) plasmid (Invitrogen). The PCR amplification of PROS1 from c.-44 to c.2028 was performed using Phusion High Fidelity (HF ...
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bioRxiv - Systems Biology 2020Quote: ... Commercial antibodies were Pvalb (Swant, GP72) and Cldn10 (Thermo Fisher Scientific, Catalog # 38-8400). Probes used in this study are Foxq1 (Advanced Cell Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti V5 tag(R960-25, Invitrogen, CA, USA), rabbit anti-RFP (mCherry ...
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bioRxiv - Microbiology 2022Quote: ... or Mouse-anti-HA tag (2-2.2.14) antibody (Invitrogen) (for HA-tagged vRNP complex pulldown experiment ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-GFP Tag (Thermo Fisher Scientific, #MA5-15256); rabbit anti-pPKA substrate (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... with an N-terminal FLAG- or HA-tag (16) or into pcDNA4/TO (Invitrogen) with an N-terminal FLAG- or EGFP-tag (17) ...
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bioRxiv - Microbiology 2020Quote: ... All human MARCH cDNAs were cloned into pCDNA3.1/myc-His A (Invitrogen) and were then subcloned into pBJ5 ...
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bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated overnight at 4°C with 100 ng of 6x His tag monoclonal antibodies (Invitrogen) in PBS and washed the next day ...
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions and final Pol I preparations were quality controlled by SDS-PAGE and Western analysis using Coomassie staining and anti-TAP-tag antibody (TAP Tag Monoclonal Antibody DyLight™ 800 4X PEG, clone 22F12-1E3, cat.no. 15342907, Fisher Scientific), respectively.
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bioRxiv - Biochemistry 2024Quote: ... anti-V5 tag (mouse monoclonal, 46-0705) and anti-V5 tag (mouse monoclonal, MA5-15253) was purchased from Invitrogen (now Thermo Fisher Scientific). anti-NUDT5 (rabbit polyclonal ...
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bioRxiv - Cell Biology 2022Quote: ... laevis Occludin from a cDNA clone purchased from Thermo Fisher (Clone ID: 7009477) and was ligated into the BglII and EcoRI sites of pCS2+/N-GFP ...
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bioRxiv - Pathology 2020Quote: ... and CD163 (LV n=26, IVS n= 25, RV n=22) for macrophages (1:800, clone EDHu-1, Thermo Scientific, Waltham, MA), all detected using the Vector Red alkaline phosphatase substrate detection kit (Vector Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... Dyrk2 cDNA clone #6808145 was purchased (ThermoFisher Scientific). Site directed mutagenesis was performed using the QuikChange Lightning Kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... A 1:10,000 dilution of mouse α-(6X)His (Invitrogen) primary antibody in PBST/3% BSA was applied for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... we cloned an EcoCas3 cDNA with a octa-histidine tag and a six asparagine-histidine repeat tag into a pFastbac-1 plasmid (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Mex67 or Sac3 under the control of the GAL1 promoter and carrying a HA C-terminal tag (Gelperin et al., 2005) were obtained from the Yeast ORF collection (Thermo Scientific Open Biosystems, YSC3868). pGP565 2µ multicopy plasmids (Jones et al. ...
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bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Biochemistry 2022Quote: ... 1024-1090 were cloned with an N-terminal 6XHis tag into the pFastBac1 vector (Invitrogen) for insect cell expression ...
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bioRxiv - Biochemistry 2021Quote: ... RANGAP1 and UBC9 ORFs were amplified from U2OS cell cDNA by high-fidelity PCR (Platinum SuperFi DNA Polymerase; Invitrogen). All constructs were generated by standard cloning or by Gibson Assembly (NEBuilder HiFi Assembly ...
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bioRxiv - Neuroscience 2022Quote: UAS-mmt stocks were generated by amplifying the mmt ORF from the corresponding cDNA and cloning it into pUASg.attB using the Gateway System (Invitrogen) and injected into embryos of yw ...
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bioRxiv - Cell Biology 2021Quote: ... rKLK10 with a 6x C-terminal His tag was affinity purified using HisPur Ni-NTA Resin (Thermo Scientific) per the manufacturer’s instruction (Figure S16 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (ACE2, dilution 1:100; TMPRSS2, 1:500; CD147, 1:500; 6x-HIS-tag (Invitrogen MA1-21315), 1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... Blots were blocked for 30 min to 1 h in 5% milk in TBST (1X Tris-buffered saline with 0.1% Tween 20) and then incubated with an anti-6X-His tag primary antibody (1:1,000 dilution, Invitrogen) (overnight ...
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bioRxiv - Cell Biology 2020Quote: ... anti-S-tag mouse monoclonal antibody (MA1-981; Thermo Fisher), rabbit polyclonal anti mNeonGreen Tag (53061S ...
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bioRxiv - Systems Biology 2022Quote: ... mouse anti-HA-tag (ThermoFisher, #26183, 1:500 for IF), mouse anti-Rab5A (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-V5 tag (Invitrogen, R960-25, 1:1:500), and mouse anti-αTUBULIN (B512 ...
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bioRxiv - Systems Biology 2024Quote: ... mouse α-V5-tag (Thermo Fisher R960-25, 1:1,000), or rabbit α-β-tubulin (Addgene ab6046 ...