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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for G CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CHO and G8 hybridomas cells were loaded respectively with 10 μM of the red cell-labeling agent DDAO (Molecular Probes) [ex/em 635/662] and 1 μM Vybrant CFDA SE [ex/em 492/517] in serum-free RPMI-1640 for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... CHO-DSP1 and MeWo-DSP2 cells were kept under puromycin selection (8 µg/ml for CHO-DSP1 and 5 µg/ml for MeWo-DSP2; Gibco). BHK-21 cells were grown in GMEM supplemented with 20 mM Hepes pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: Chinese Hamster Ovary (CHO) cells were maintained similar to HEK cells with the following differences: Culture media was IMDM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... G-MEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Fluoromount-G (Invitrogen) was used to prepare the coverslips for cell imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Fluoromount-G (Invitrogen) containing DAPI was used for mounting IHC stained slices ...
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bioRxiv - Neuroscience 2023Quote: ... Fluoromount-G (Invitrogen) was used to mount coverslips (Vetrini Coprioggetto ...
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bioRxiv - Immunology 2021Quote: ... containing the cytokines IL-4 (500 U/mL) and GM-CSF (800 U/mL) (both Gibco) for differentiation into DCs ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated in artificial CSF buffer containing bis(sulfosuccinimidyl)suberate (BS3) cross-linker (2 mM, ThermoFisher) for 30 min to 4 h at 4°C with constant invert mixing ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Neat CSF samples (10 μL) were mixed with equal volume PrepMan™ Ultra (Thermo-Fisher Scientific) reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Neuroscience 2024Quote: Peptides extracted from CSF were analyzed with an Orbitrap Q-Exactive HF mass spectrometer (Thermo Scientific) coupled with an online Easy-nLC 1200 nano-HPLC system (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... 50 μl protein G beads (Dynabeads Protein G, Invitrogen, Cat: 10004D) were coupled with 2.5 μg serum IgG from Ctr or DC-LMP1/CD40 mice for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... plasmids encoding targeted proteins were transiently transfected into ExpiCHO-S cells by using ExpiFectamine™ CHO transfection kit (Thermo Scientific, A29129). Transfected cells were cultured in stackable CO2 incubator shaker (Kühner AG ...
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bioRxiv - Microbiology 2021Quote: ... 100 µg of plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Bioengineering 2021Quote: CHO cells and CHO EVs for SEM were fixed in 4% EM grade glutaraldehyde overnight on poly-L-lysine coated glass coverslips (Fisher Scientific). Samples were processed for SEM imaging by treating the samples to 1% osmium tetroxide for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... CHO cells stably expressing LI-cadherin-GFP were established using Flp-In™-CHO Cell Line following the manufacturer’s protocol (ThermoFisher Scientific). Cloning was performed by the limiting dilution-culture method ...
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bioRxiv - Microbiology 2019Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Biochemistry 2021Quote: ... One ampule of cells from a working cell bank was thawed in CD-CHO basal medium (Thermo Fisher Scientific, Waltham, MA), along with 0.5 % anti-clumping agent (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ExpiFectamine CHO Enhancer (6 μl per ml of culture) and ExpiCHO Feed (240 μl per ml of culture) (Thermo Fisher Scientific) were added 18-22 hours post-transfection and the temperature was decreased to 33 °C ...
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bioRxiv - Microbiology 2019Quote: ... EDE2 B7 (Dejnirattisai et al., 2015), CR4354 (Kaufmann et al., 2010) were expressed by transient transfection of Expi-CHO-S cells (Cat# A29129; ThermoFisher Scientific). Variable heavy and light chain sequences of the above control mAbs used for gene synthesis and cloning into expression vectors were based on PDB IDs 4UT9 ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamine CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
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bioRxiv - Microbiology 2022Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Transfections of NIH 3T3 and CHO cells were carried out using a Neon MP-100 microporator system (Life Technologies, Carlsbad, CA). Transfection of HEK293 cells was performed using the calcium phosphate method ...
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bioRxiv - Neuroscience 2023Quote: Chinese hamster ovary (CHO) cells were maintained in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... trimer was produced and differentially purified from a CZA97.012 SOSIP.664-expressing CHO-cell line maintained in ExpiCHO Expression Medium (Thermo Fisher Scientific) (82) ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and GlyA-CHO cells (glycine auxotrophic for growth and a gift from Dr. P. J. Stover, Cornell University) using Lipofectamine 2000 (Invitrogen # 11668019). Briefly ...
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bioRxiv - Genomics 2023Quote: A mAb producing CHOK1 cell line (CHO K1 mAb) was seeded at a density of 2 × 105 cells/ml in 50ml SFM-II media (Gibco, 12052098) in 8 replicate shake flasks in a Kuhner orbital shaker at 170rpm at 5% CO2 ...
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bioRxiv - Immunology 2022Quote: The pcDNA3.1 plasmids encoding S and S-Fc were transfected into CHO cells using PEI MAX 40000 (Fisher Scientific, Cat# NC1038561). Stable cell lines were selected and maintained under G418 (1 mg/ml) ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Immunology 2024Quote: ... and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA ...
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin antibody complexes were isolated using protein A-beads for rabbit primary antibodies or G-beads for mouse primary antibodies (Dynabeads, Invitrogen). The PCRs with 1:20 dilutions of genomic DNA (input ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected using 1 μg/ ml of anti-rabbit Alexa Fluor 594 or anti-mouse Alexa Fluor 488 in 1%BSA as appropriate (Invitrogen). All antibodies were incubated at room temperature for 1 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-TRF1 antibody (Goat Pab sc-1977, SantaCruz), or anti-PAR (Mouse Mab 10H ALX-804220, Alexis) recovered with Protein-G dynabeads (Invitrogen), run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins ...
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bioRxiv - Immunology 2019Quote: ... 0.1 ml/10 g mouse) and the liver was cannulated via the portal vein and perfused with 20 mL HBSS (Gibco) containing 0.5 mM EGTA (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... rinsed and incubated with secondary antibody Alexa Fluor 594 Goat Anti-mouse immunoglobulin G (IgG) (H/L) antibody (1:1000, Invitrogen) for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase (AP)-labeled goat anti-mouse IgG (H+L) cross-adsorbed secondary Ab (G-21060) was from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2020Quote: ... The following day the slices were incubated with a primary monoclonal mouse antibody against Ankyrin G (1:100; Thermo Fisher Scientific Cat# 33-8800 ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were washed with PBS and incubated for 1 hour with secondary antibody [Alexa Fluor-488 goat anti-mouse immunoglobulin G (IgG) (H+L) conjugate or anti-rabbit IgG (H+L) conjugate (Invitrogen)] ...
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bioRxiv - Neuroscience 2021Quote: NAc samples were pooled from four D1-Cre-RT or D2-Cre-RT male and female mice and RNA isolation from immunoprecipitated polyribosomes using primary mouse anti-HA antibody (Covance Research Products, Cat#MMS101R) and secondary antibody coated magnetic Dynabeads (Dynabeads protein G, Invitrogen) to pulldown the MSN specific RNA ...
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bioRxiv - Biochemistry 2020Quote: ... cells were simultaneously incubated with secondary Alexa Fluor 488 goat anti-mouse immunoglobulin G (IgG) (Invitrogen, A-11001, 1:1000) and Streptavidin-Alexa Fluor 647 IgG (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... blots were incubated with secondary HRP-conjugated goat anti-mouse IgG antibodies (Thermo Fisher Scientific Cat# G-21040, 1:10000). Chemiluminescence signal was generated using Clarity ECL substrate (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-mouse HRP-conjugated (G-21040, 1:10000) and anti-rabbit HRP-conjugated (65-6120, 1:10000) secondary antibodies from Invitrogen.