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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for G CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Goat anti-mouse and goat anti-rabbit immunoglobulin G (IgG) antibodies conjugated to Alexa Fluor 488 (Life Technologies) and diluted at 1:100 were used ...
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bioRxiv - Plant Biology 2021Quote: ... followed by a HRP-conjugated goat anti-mouse IgG (H+L) Secondary Antibody (G-21040, Thermo Fisher Scientific) in soybean ...
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bioRxiv - Cell Biology 2020Quote: ... Dy-Light 488-conjugated rat anti-mouse GPIbβ Ab (0.15µg/g;Emfret) and Alexa 647-conjugated fibrinogen (5% total fibrinogen; Invitrogen) were administered via a cannula inserted in the jugular vein ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor-488- or 555 or 647-conjugated anti-mouse or rabbit or chicken immunoglobulin G (IgG) (Invitrogen) were used at a dilution of 1:500 ...
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bioRxiv - Microbiology 2024Quote: ... or 2 μg of Flag Tag antibody (mouse) conjugated with Dynabeads Protein G Magnetic Beads (Invitrogen, 10004D, USA), or anti-HA magnetic Beads (Abmart ...
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bioRxiv - Biochemistry 2019Quote: ... Positive screened samples were initiated to production by the addition of 20% CHO CD EfficientFeed B (Thermo Fisher, A1024001) and a temperature shift to 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... were seeded at 0.3 x 106 cells/mL in 3 L (total working volume) of CD-CHO medium (Invitrogen) supplemented with 50 µg/L rMSB ...
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bioRxiv - Microbiology 2020Quote: rS1-Fc was expressed in Chinese hamster ovary cells (Free Style CHO-S Cells by Thermo Fisher Scientific, # R80007) in CHO-S-SFM media with hypoxanthine and thymidine (Life Technologies ...
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bioRxiv - Immunology 2022Quote: Vaccine 5: The above mentioned hCMP-mRBD antigen when expressed from Stable CHO (Thermo Fisher Scientific, Cat no. R75807) cell lines was named as hCMP-mRBD-CHO ...
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bioRxiv - Biochemistry 2022Quote: Chinese hamster ovary (CHO) cells (K1 line, ATCC # CRL-61) were cultured in Glasgow’s modified Eagle’s medium (GMEM, Life Technologies), 10% fetal bovine serum (GIBCO) ...
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bioRxiv - Physiology 2020Quote: ... The CHO-K1 cells stably expressing HCN4 were grown in Ham’s F12 medium with L-glutamine (Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: CHO K1 WT cells (ATCC, #CCL-61) were maintained in F-12 Nut Mix + GlutaMAX (#31765-027, Thermo Fisher) at a subconfluent level and passaged every 4 days ...
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bioRxiv - Microbiology 2022Quote: ... plasmids (1,000◻ng per ml of culture) were transfected in ExpiCHO-S cells using ExpiFectamine CHO (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CHO cells that were stably transfected with human CTLA-4 have been reported.23 CHO cells were grown in DMEM (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Synthetic Biology 2020Quote: ... with 4.5 g/L glucose (1 g/L, Gibco #31600-091 ...
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bioRxiv - Neuroscience 2020Quote: ... 9.4% (1.023 g/ml) and 7% (1.017 g/ml) OptiprepTM (1.320 g/ml) (Thermo Fisher Scientific). After centrifugation at 800 x g for 15 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were next differentiated to moDCs using human rGM-CSF (20ng/ml, BioCource/Life technologies) and rIL-4 (0.86ng/ml ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 20 ng/ml GM-CSF (Preprotech, Thermo Fisher Scientific, USA) and 10 ng/ml IL-4 (Preprotech ...
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bioRxiv - Microbiology 2023Quote: ... and m7 G(5′)ppp(5′)G Cap Analog (Ambion). BHK-21 cells in 6-well plates were transfected with the capped-SINV-AaBec243-260-mCh-Flag or capped-SINV-mCh-Flag mRNA using Lipofectamine LTX (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies (Alexa Fluor 488, 594-conjugated goat anti-rabbit or anti-mouse immunoglobulins G (IgGs) (1/1,000; Invitrogen) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 293T and mouse hepatoma (Hepa1c1c) cells were cultured in DMEM (4.5 g l-1 glucose, Hyclone) supplemented with 10% FBS (Gibco) and 100 units (penicillin) ...
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bioRxiv - Biochemistry 2021Quote: N2a mouse neuroblastoma cells (ATCC) and C2C12 (ATCC) cells were cultured in DMEM containing 4.5 g/l glucose (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... followed by a horseradish peroxidase (HRP)-labeled Goat anti-Mouse IgG (cat # G-21040, Thermo Fisher, Waltham, MA, USA) and TrueBlue Peroxidase Substrate (cat # 5510-0030 ...
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bioRxiv - Cell Biology 2021Quote: ... AMPKα-null MEFs and NIH-3T3 mouse fibroblasts were cultured in low glucose DMEM (2 g/L, from Gibco), with 10% filtered fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... mouse monoclonal or anti-GFP rabbit polyclonal (ab290) antibody was incubated with Protein-G/A magnetic beads (Dynabeads, Invitrogen) in PBST for 2hrs at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... A secondary antibody (donkey anti-mouse coupled with Alexia 488 Ig G [H+L]; Life Technologies, Thermo Fisher Scientific) diluted at 1:300 was incubated for 2 h on slides ...
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bioRxiv - Cell Biology 2021Quote: ... A secondary antibody (donkey anti-mouse coupled with Alexia 488 Ig G [H+L]; Life Technologies, Thermo Fisher Scientific) diluted at 1:300 was incubated for 2 h on slides ...
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bioRxiv - Microbiology 2023Quote: ... and goat anti-mouse IgG (G-21040) secondary antibodies conjugated to horseradish peroxidase (HRP) were used at 1:5,000 (ThermoFisher Scientific Invitrogen). SuperSignal West Pico Chemiluminescent Substrate was used to detect HRP on immunoblots per manufacturer instructions (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... then incubated with Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody conjugated to HRP (ThermoFisher, #G-21040) at a 1:350 dilution in 1X PBS with 0.05% Tween® 20 and 1% fetal bovine serum overnight at 37°C at 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 times with TBS-T and incubated 1h at RT with an HRP-conjugated anti-mouse secondary antibody (dilution 1:50,000, G-21040, Invitrogen). After 3 washes in TBS-T ...
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells stably expressing I-Ek-MCC (CHO-I-Ek) were maintained by passage every 3 days in complete RPMI 1640 medium (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... ExpiCHO-S cells were transfected with full-length pRTL1-GCSF vectors using the ExpiFectamine™ CHO Transfection Kit (Life Technologies Corporation ...
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bioRxiv - Genetics 2021Quote: ... The hGOT1-GFP and mutant hGOT1-GFP constructs were transfected into Chinese Hamster Ovary (CHO) (ExpiCHO-S) cells with an ExpiFectamine Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing the Fab heavy chains and light chains were chemically co-transfected into an afucosylated ExpiCHO cell line using the ExpiFectamine CHO Transfection Kit (Gibco). After 9 days of transient expression ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Biochemistry 2021Quote: The wild-type (WT) and mutant GLP-1R constructs were integrated into FlpIn-CHO cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Stable Flp-In-CHO cell lines were generated through co-transfection of the pcDNA5/FRT expression vector (Thermo Fisher Scientific) containing the gene of interest and the Flp recombinase expressing plasmid ...
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bioRxiv - Microbiology 2021Quote: Plasmid pCAG-CD5-S-ecto-6P-Spytag was transiently transfected into ExpiCHO cells using ExpiFectamine CHO transfection kit (Thermo Fisher). After 18-22 hr of transfection ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into HEK293T cells (3×105/well) using PEI or into CHO cells (1.5×105/well) with lipofectamine 3000 reagent (ThermoFisher, L3000001) in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 and CHO cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12; Gibco, #11320033), supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing SARS-CoV-2 S-glycoprotein were seeded in 96 well plates for microscopy (Thermo Fisher Scientific) at 12’500 cells/well and the following day ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: CHO cell lines overexpressing FPR2 and FPR1 receptors were generated in-house and propagated in Ham’s F12 medium (Gibco Biosciences) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA from CHO-FPR2 overexpressing cells or HL-60 cells was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and purified using RNeasy Mini kits (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Biophysics 2022Quote: ... and a combination of the glCV30 Hc and glCV30 Lc vectors or a combination of the glCV30-SDF-1α Hc and glCV30 Lc vectors were introduced into the CHO-S cells (Gibco). The transfected cells were grown in the ExpiCHO expression medium (Gibco ...
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bioRxiv - Physiology 2023Quote: Myc-tagged wild-type (WT) and PKA-resistant mutant (Ser791Ala) Raptor vectors7 were transfected into CHO-GLP-1R cells using lipofectamine-3000 (Invitrogen). 48h later ...
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bioRxiv - Immunology 2022Quote: ... expansion and production of the CHO cells from the working cell bank (WCB) into shake flasks and bioreactor (Applikon) using serum-free medium (Gibco). At the end of the growth phase ...