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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for G CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Immunology 2024Quote: ... and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA ...
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bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were re-suspended in 5ml culture medium plus macrophage colony stimulation factor (M-CSF) (Affymetrix eBioscience) at a concentration of 50ng/ml and counted on a Nucleocounter NC-250 ...
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bioRxiv - Bioengineering 2020Quote: ... GM-CSF differentiated mBMDCs were cultured by growing murine bone marrow-derived cells in RPMI media (Invitrogen) with 10% characterized fetal bovine serum (HyClone ...
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bioRxiv - Bioengineering 2019Quote: ... and macrophage colony-stimulating factor (M-CSF, Gibco, 1µg per 5mL medium, Cat. no. PHC9501, Gibco®) in a T-25 culture flask for 5 days.
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bioRxiv - Cell Biology 2021Quote: ... 100 μL CSF-arrested or interphase extract reactions were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 5 μg rabbit anti-xCENP-C antibody ...
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bioRxiv - Neuroscience 2022Quote: OPG in the CSF was measured using the Human TNFRSF11B(OPG) Elisa kit (Thermo Fisher Scientific, Germany). The assay was performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... purified HIVJR-CSF gp120 protein was biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) and excess biotin was removed using a Zeba spin desalting column (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescent CSF tracer (albumin-Alexa Fluor™ 555, Cat No. A34786, Thermo Fisher Scientific, Waltham, MA, USA) was added to the artificial CSF at a concentration of 5 mg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D). 30 µL of beads were used per IP ...
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bioRxiv - Neuroscience 2021Quote: ... G-a-mIgG2b A647 and G-a-mIgG1 CF680 (1:200, ThermoFisher), diluted in 0.1% BSA/BPS ...
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bioRxiv - Cell Biology 2021Quote: ... followed by preclearance using protein G-beads (protein G from Thermo Fisher). 500 μg of each precleared lysate was incubated with anti-MET antibody and protein-G beads for overnight at 4°C with rotation ...
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bioRxiv - Neuroscience 2021Quote: ... G-a-mIgG2b A647 and G-a-mIgG1 CF680 (1:200, ThermoFisher), diluted in 0.1% BSA/BPS ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Batch cultivation was performed in 250 mL shake flasks containing 60-80 mL CD-CHO medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 8 mM L-glutamine (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... microscale transfection were performed (∼1 ml per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2021Quote: ... were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Expression SΔTM trimers was performed by transfection of ExpiCHO-S™ cells with the plasmids described above using the ExpiFectamine™ CHO transfection kit (Gibco™ ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamine™ CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... The plasmid expression plasmid carrying each SARS-CoV-2-associated protein was transiently transfected into ExpiCHO-S cells by using the ExpiFectamine™ CHO transfection kit according to the manufacturer’s instruction (Thermo Scientific, A29129). After transfection ...
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bioRxiv - Immunology 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently expressed in Expi-CHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... IgG or Fab were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... GenBank accession number NM_004519) was generated using Flp-In™-CHO cells according to the supplier’s protocol (Thermo Fisher Scientific, Waltham, USA). CHO-Q3 stable cells were maintained under dual selection with Zeocin (100 μg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... To identify these target cells during downstream analysis CHO DP-12 cells were first stained with CellTracker™ Blue CMAC Dye (Thermo Fisher). 85 µm nanovials containing RGD and cells were seeded into a 12-well plate as described above and then incubated at 37°C for 2 hours to allow cells to adhere to the particles ...
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bioRxiv - Immunology 2020Quote: ... and soluble cytosolic protein (SCP) fractions were extracted from Chinese hamster ovary (CHO) cells and biotinylated using NHS-LC-Biotin (Thermo Fisher Scientific) reagent ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Expression plasmids encoding the heavy and light chains of the COVA1-16 Fab were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... microscale transfection was performed (∼1 mL per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2020Quote: ... microscale transfection were performed (~1 ml per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2022Quote: Recombinant human β2m-MR1-K43A-Fc was produced in CHO-K1 cells as previously described (10) and 4 µg/mL were coated onto 96 wells plates (Nunc, Cat#439454) for 18 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... These confluent CHO cells were transfected with 700 ng pUNO1-Mincle or pUNO1-DC-SIGN using TurboFect Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The AF-03 and AF03-NL plasmid were transfected into ExpiCHO-S cells using the ExpiFectamine™ CHO Transfection Kit (Gibco, A29129) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... microscale transfection was performed (∼1 ml per antibody) with CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ T cells were co-cultured with CHO cells for 16 hours in the presence of T cell activation using anti-CD3/CD28 Dynabeads™ (ThermoFisher Scientific) at an estimated 1:1 bead-to-cell ratio ...
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bioRxiv - Physiology 2020Quote: Dynabeads Protein G (Invitrogen) were incubated with 5 μg of antibody in 200 μl TBS with 0.1% Tween (TBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... G (10002D, 10004D, Invitrogen). Immunoprecipitated proteins were digested on beads by trypsin (V5280 ...
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bioRxiv - Microbiology 2021Quote: ... Protein G Dynabeads (Invitrogen) were then added and incubated overnight at 4°C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... and VSV-g (Invitrogen)] ...
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bioRxiv - Neuroscience 2020Quote: ... with Fluoromount-G (Invitrogen). Confocal acquisitions were performed with an SP-5 Leica microscope (x40) ...