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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for G CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells with positive expression of the antibody were grown in CD CHO AGT expression medium (ThermoFisher) with supplemental glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... Positive transfected cell pools for both trimers were selected in serum-free medium (CD CHO (Gibco), supplemented with selection medium (SM ...
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bioRxiv - Cell Biology 2021Quote: ... and CHO cells were transfected using Lipofectamine 3000 and the P3000TM Enhancer Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The CHO-2 cell line growth medium contained 7.5 µg/mL puromycin (Thermo Fisher, Catalogue # A1113802) and each of the cloning media tested for CHO-2 were supplemented with 7.5 µg/mL puromycin accordingly.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CHO cells stably expressing TRPV4 (43) were cultured in Ham’s Nutrient Mixture F12 (Thermo Fisher Scientific), supplemented with 10% FBS ...
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bioRxiv - Biophysics 2022Quote: CHO-K1 cells were cultured in phenol-red free HF12 (Himedia) supplemented with 10% FBS (Gibco) and 1% antibiotics (Gibco) ...
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bioRxiv - Systems Biology 2023Quote: ... and transferred to a conical tube containing 5 mL of 1X CD CHO Medium (Gibco, 10743011) bubbled with carbogen ...
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bioRxiv - Bioengineering 2023Quote: Host cell DNA were analyzed using resSEQ™ CHO DNA Quantification Kit (Thermo Fisher Scientific, USA) following supplier’s protocol ...
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bioRxiv - Bioengineering 2023Quote: Host cell Protein were analyzed using ProteinSEQ™ CHO HCP Quantitation Kit (Thermo Fisher Scientific, USA) as per supplier’s protocol ...
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A molecular switch in RCK2 triggers sodium-dependent activation of KNa1.1 (KCNT1) potassium channelsbioRxiv - Biophysics 2023Quote: ... Chinese hamster ovary (CHO) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco, Paisley, UK) supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2023Quote: ... Raji cells and CHO cells were cultured in complete RPMI-1640 (Gibco, 10% FCS, 2mM GlutaMAX), and HEK293T cells ...
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bioRxiv - Neuroscience 2024Quote: ... pCMV-TMEM63B-IRES-eGFP construct was transfected into CHO cells using lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CHO cells stably expressing human CB1R were harvested by incubation with Versene (ThermoFisher Scientific, Waltham, MA) for 10 min ...
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bioRxiv - Immunology 2024Quote: ... CHO cells were kept at 37°C and 5% CO2 in OptiMEMTM with GlutamaxTM (Life Technologies) supplemented with 10% heat-inactivated endotoxin-low FCS ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary antibodies used were Alexa546 or Alexa488 coupled goat anti–mouse immunoglobulin G (IgG) (Life Technologies), and horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... The antibody for p130 and mouse IgG were incubated with the Dynabeads Protein G (Invitrogen, 10003D) for 4 hrs at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... secondary Alexa Fluor goat anti-mouse immunoglobulin G (IgG) 594 (1:5000; Invitrogen, A11032, lot 1985396), and secondary Alexa Fluor goat anti-mouse IgG 488 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... growth media containing DMEM (HEK293) or DMEM/F12 (CHO) (both high glucose, pyruvate, Thermo Fisher Scientific (TFS)) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: BHK (ATCC), A431 (ATCC), MEF (as described in (Meiring et al., 2019)) and Flp-In-CHO (Invitrogen) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) ...
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bioRxiv - Biophysics 2021Quote: ... Chinese Hamster Ovary (CHO) cell lines were a Tetracycline-Regulated Expression (T-REx) variant (Invitrogen, Cat# R71807), and cultured as described previously (47) ...
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bioRxiv - Genetics 2021Quote: ... CHO-K cells were cultured in F-12K Medium (Kaighn’s Modification of Ham’s F-12 Medium) (Gibco) supplemented with 10% Fetalgro® Bovine Growth Serum (BGS) ...
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bioRxiv - Cell Biology 2022Quote: CHO cells were kept at 37°C and 5% CO2 in OptiMEM™ + Glutamax™ (Life Technologies) supplemented with 10% heat-inactivated endotoxin-low FCS ...
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bioRxiv - Immunology 2023Quote: Chinese hamster ovary (CHO) cells transfected with CXCR3A were cultured in Ham’s F-12 growth medium (Gibco; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Cell Biology 2019Quote: ... Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn ...
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bioRxiv - Microbiology 2019Quote: ... 5% heat-inactivated AB human serum and 20 ng/mL M-CSF (Invitrogen). Cells were washed with PBS and resuspended in fresh media on days 3 and 6 of differentiation ...
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bioRxiv - Systems Biology 2020Quote: ... The following antibodies and dilutions were used: M-CSF Polyclonal Antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were further stimulated with GM-CSF (2000 IU/mL, Gibco, MT, USA) and IL-4 (1000 IU/mL ...
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bioRxiv - Immunology 2022Quote: ... medium supplemented with 10 ng/ml human GM-CSF and 20% FCS (Gibco). For the purposes of proliferation assays ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were further stimulated with GM-CSF (2000 IU/mL, Gibco, MT, USA) and IL-4 (1000 IU/mL ...
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bioRxiv - Microbiology 2023Quote: ... 4 g/L dextrose, 5.957 g/L HEPES, 0.05 g/L hypoxanthine, 5 g/L Albumax II, Invitrogen) supplemented with 200 mM L-glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... were used for mouse antibodies and protein G beads were used for rabbit antibodies (Dynabeads, Invitrogen, 10004D). Antibodies used were to CENP-A69 and CENP-C (gift from W ...
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bioRxiv - Developmental Biology 2021Quote: 1μl Mouse anti-V5 antibody was added to every 10μl Dynabeads™ protein G (Thermo Fisher Scientific) (2.5μl per sample ...
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bioRxiv - Microbiology 2021Quote: ... Purified mouse IgGs at w11 were obtained using a 0.2-ml protein G spin kit (Thermo Scientific) following the manufacturer’s instructions and assessed in pseudovirus neutralization assays ...
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bioRxiv - Neuroscience 2022Quote: ... including donkey anti-mouse immunoglobulin G conjugated to Alexa Fluor 594 dye (Invitrogen A-21203, RRID: AB_141633) and donkey anti-rabbit immunoglobulin G conjugated to Alexa Fluor 488 dye (Invitrogen A-21206 ...
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bioRxiv - Molecular Biology 2022Quote: ... or mouse IgG-1κ (for negative control) conjugated to Protein A/G UltraLink Resin (Thermo Fisher Scientific) at room temperature for 2 h with gentle rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: Alexa Fluor 488 donkey anti-mouse immunoglobulin G (1:200, Thermo Fisher Scientific; A-21202) and Alexa Fluor 555 donkey anti-rabbit immunoglobulin G (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the heavy and light chains were co-transfected into FreeStyle™ CHO-S™ cells (Invitrogen) in serum free FreeStyle™ CHO™ Expression medium ...
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bioRxiv - Systems Biology 2019Quote: ... were seeded at 3 × 105 cells per mL in 60 mL CD-CHO medium (Thermo Fisher Scientific, USA) supplemented with 8 mM L-Glutamine (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... Wild type Chinese ovarian hamster cells (CHO) were transfected with the respective plasmids using TurboFect (Thermo Fisher Scientific) according to manufacturer’s instructions and selected with geneticin (VWR ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Chinese hamster ovary cells (CHO) (RRID: CVCL_0214) were grown at pH 7.4 in filtered sterile F12 (Ham’s) nutrient medium (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Following electroporation (300 V, 900 μF) cells were resuspended in 100 mL glutamine-free CD CHO medium (ThermoFisher). The following day ...
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bioRxiv - Cancer Biology 2023Quote: The codon-optimized for CHO variable light and heavy chains (02B11, atezolizumab, and durvalumab) were synthesized (ThermoFisher Scientific) and then cloned into pTRIOZ-mIgG2a/mkappa (κ ...
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bioRxiv - Cancer Biology 2023Quote: ... expressing the Flp recombinase to produce stably transfected Flp-InTM CHO cells (Thermo Fisher Scientific, Waltham, MA, USA). In order to overexpress human PAMR1 isoform 1 in HT29 cells ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Invitrogen CHO K1 cell line with CellSensor® construct (TrkB-NFAT-bla, K1491) was obtained from Life Technologies and cultured in growth medium (DMEM with GlutaMax ...
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bioRxiv - Genetics 2021Quote: ... The CSF sample was immediately placed inside a freezing container (Mister Frosty, Thermo Fisher) and stored in the -80 °C freezer overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 ng/mL GM-CSF in Advanced RPMI-1640 (Gibco; Cat. No. 12633012)) ...
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bioRxiv - Microbiology 2019Quote: ... Macrophages were then isolated by plastic adherence and differentiated using GM-CSF (Thermo Fisher). CD4+ T cells were isolated using an EasySep kit (StemCell ...
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bioRxiv - Immunology 2021Quote: ... at 4°C with 2µg anti-CD3 (mouse: eBioscience 14-0031-86; human: eBioscience 16-0037-85) and 1µg anti-CD28 (mouse: Invitrogen 14-0281-86; human: 16-0289-85) and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse ...