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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... miRNA-147-3p or miR-298-5p mimics (Dharmacon) and incubated for 72 hours in DMEM/OptiMEM medium (Sigma-Aldrich/Thermo Fisher Scientific) prior lysis.
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bioRxiv - Cell Biology 2021Quote: Transfection procedure of pre-miR-145 in Calu-3 cells was performed in 12-well plates using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen, Thermo Fischer Scientific) accordingly to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... miRNA-mediated protein knockdown was performed as described previously (Alexander and Hammer, 2016) using the BLOCK-iT Inducible Pol II miR RNAi Expression Vector Kit (Life Technologies K4939-00). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg of each pmirGLO construct was transfected into human HEK-293T cells [44] (6 × 104 cells/well) with 1.6 pmol of miR-155 or scrambled control miRNA (Thermo Scientific Dharmacon, Waltham, MA) in a 96-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... has been used for HeLa cell line transfection with plasmid DNA or with 100nM of miRVANA miR-184 inhibitor or 100 nM negative control miRNA inhibitor (cat. 4464076-Invitrogen, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2022Quote: Four oligonucleotide sequences for the miR-NRF2-based shRNAs targeting have been designed using the Block-iT RNAi designer tools (Invitrogen; Supplementary Table 4) and cloned into the pcDNA6.2-GW /EmGFPmiR plasmid according to the manufacturer protocol (BLOCK-iT polII miR RNAi ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Then cDNA were produced from 5 μL of total RNA using RT Superscript III (RT Superscript III First Strand cDNA Synthesis Kit; Invitrogen, Life Technologies) and random hexamer primers (50 ng ...
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bioRxiv - Immunology 2022Quote: Isolated RNA was converted to complementary DNA by reverse transcription (RT) with random hexamers and Multiscribe RT (TaqMan Reverse Transcription Reagents; Thermo Fisher Scientific). For IFITM2 expression assays ...
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bioRxiv - Genomics 2020Quote: ... The plate was heated at 65° C for 5 minutes and transferred to ice prior to the addition of 1 ul of 2X Reverse transcription mix (15% PEG 8000 [Sigma Aldrich], 2X Maxima RT buffer [Thermo Fischer] and 4 units of Maxima H Minus RT [Thermo Fisher]). cDNA synthesis was performed for 15 min at 50°C followed by 5 min at 85° C for inactivation ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS at RT) and incubated with secondary antibodies for 1 hour at RT: AlexaFluor-488 goat anti-mouse (1:2000; ThermoFisher Scientific, A11029) or AlexaFluor-594 donkey anti-rabbit (1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... The RT-qPCR assay were performed with a SuperScript III Platinum One-Step RT-qPCR Kit with ROX (Invitrogen - Thermo Fisher Scientific) on a QuantStudio 12K Flex thermocycler (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... The RT-qPCR assay were performed with a SuperScript III Platinum One-Step RT-qPCR Kit with ROX (Invitrogen - Thermo Fisher Scientific) on a QuantStudio 12K Flex thermocycler (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: We adjusted RNA concentrations and performed Reverse-Transcription (RT) on extracted RNAs using a SuperScript II RT kit (Thermofisher, Waltham, Massachusetts, USA) and random primers (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Isolated RNA was converted to complementary DNA by reverse transcription (RT) with random hexamers and Multiscribe RT (TaqMan Reverse Transcription Reagents, Thermo Fisher Scientific). The reactions were set up using the manufacturer’s guidelines and run on a QS5 Studio Real-Time PCR instrument (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 µl AMV RT (12U final, ThermoFisher 18080044) were added to each reaction for a final volume of 20 µl ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using PowerSYBR Green (Applied Biosystems) with AdV specific primers ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl Maxima H Minus RT (Thermo Scientific. EP0742) and nuclease-free water up to 20 µl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... High Capacity cDNA RT kit were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Microbiology 2019Quote: ... RT was performed using 50uM Oligo(dT) (Thermo Fisher) as previously described [49] with an 48°C extension temperature ...
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bioRxiv - Genetics 2019Quote: ... consisting of: 2μL 5x RT buffer (Thermo Fisher Scientific), 2μL 5 M Betaine (Sigma-Aldrich) ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was done using SuperScript II RT (Invitrogen) and qRT-PCR was performed using SYBR FAST Universal 2X qPCR Master Mix (Kapa ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and SuperScript III RT (Invitrogen, Cat. No. 18080-044). RNA template was digested from the cDNA using 2 units of E ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 µl AMV RT (12U final, ThermoFisher 18080044) were added to each ligation reaction to the final volume of 20 µl and incubated for 1 h at 42°C ...
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bioRxiv - Plant Biology 2021Quote: ... using RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific).
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bioRxiv - Physiology 2020Quote: ... 1μL Superscript III RT® (18080-044, Life Technologies) and 1μL RNaseOUT® (10777-019 ...
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bioRxiv - Cell Biology 2022Quote: Prepare RT reaction with SuperScript III (Thermo Fisher – 18080044). (all 12 μL of RA3 adapter-ligated sample ...
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bioRxiv - Microbiology 2020Quote: ... cDNA-synthesis was performed using Multiscribe RT (Applied Biosystems) with 10μl of RNA input (supplementary table 2) ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions with TaqPath master mix (Thermo Fisher) were assembled following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 0.75 μl Maxima RT Minus H (Thermo Scientific, EP0751), 1.8 μl 5M Betaine (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... RT reaction was carried out with SuperScript III (Invitrogen), SuperScript IV (18090010 ...
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bioRxiv - Systems Biology 2021Quote: ... and RT-qPCR (Thermo-Fisher Scientific PowerUp SYBR green) were performed as described previously54 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2.7 µl RT mix containing SuperScript II buffer (Invitrogen), 9.3 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... MuLV RT enzyme and random hexamer (Thermo Fisher Scientific) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions with TaqPath master mix (Thermo Fisher) were assembled following the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... RT-qPCR was performed with Power SYBR-green (ThermoFisher) with the standard protocol (Tm = 60°C for 1 min ...
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bioRxiv - Genomics 2019Quote: ... RT was performed using SuperScript IV (Thermo Fisher Scientific) and a gene-specific primer ...
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bioRxiv - Immunology 2019Quote: ... 2.7 μl RT mix containing SuperScript II buffer (Invitrogen), 9.3mM DTT ...
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bioRxiv - Immunology 2021Quote: ... and a Superscript III RT/Platinum Taq Mix (Invitrogen), with the following conditions ...
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bioRxiv - Immunology 2021Quote: ... and a Superscript III RT/Platinum Taq Mix (Invitrogen), with the following conditions ...
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bioRxiv - Genomics 2022Quote: ... and mixed with 2μl 10x RT buffer (ThermoFisher Scientific), 4μl 25mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... and reverse transcribed with SuperScript III RT (Thermo Fisher) per manufacturers guidelines using a gene specific primer specific to the transcript of interest ...
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bioRxiv - Plant Biology 2022Quote: ... 4 mM dNTP mix and 1x RT buffer (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RT-qPCR were run on QuantStudio 3 (Applied Biosystems) using PowerUpTM SYBRTM green master mix with specific primers ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed with a StepOnePlus (Applied Biosystems) for 40 cycles (SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using Superscript IV RT polymerase (Invitrogen), RNA pol inhibitor (Applied Biosystems) ...
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bioRxiv - Genomics 2024Quote: Maxima 5X RT buffer (Thermo Fisher, cat. no. EP0751)
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bioRxiv - Immunology 2024Quote: ... Complementary DNA was prepared using M-MLV RT (Invitrogen), and quantitative PCR (qPCR ...
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bioRxiv - Genomics 2024Quote: ... RT-qPCR was carried on QuantStudio 7 Flex (ThermoFisher). Fold change differences were plotted as 2-ΔCt.
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bioRxiv - Immunology 2022Quote: ... Taq Path 1 step RT-qPCR Master (Applied Biosystems) and CoV RUO positiv control kit (IDT ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was reverse transcribed by SuperScript III RT (Invitrogen) with RP1 Illumina primer to generate cDNA libraries ...