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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding KNL12026-2342 and ZWINT wild-type and mutants were cloned into a pCDNA5/FRT/TO-EGFP-IRES plasmid and co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
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bioRxiv - Biochemistry 2023Quote: ... and human phosphatidylinositol 5-phosphate 4-kinase type-2 beta (hPIP4KB; Uniprot #P78356) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). Gene sequences were subcloned into either bacterial ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded at the final density of 2×106 cells/ml in the central chamber of the DAX-1 chips embedded in a matrix composed of type I rat tail collagen at the final concentration of 2.3 mg/ml (Thermofisher, #A1048301). Each chip can be loaded with 10µl of gel ...
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bioRxiv - Cancer Biology 2023Quote: ... and the tumor or para-tumor tissues collected from USP39HKI and wild- type (WT) mice were suspended in TRIzol reagent (Invitrogen) and sent to Novogene for RNA sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... This suspension was in turn dissolved in a mixture of 50% Collagen type I (4mg/ml; Thermo Fisher Scien-tific), 25% Matrigel ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were plated on 0.4% gelatin-coated 60 mm culture dishes (Gelatin Type B Powder, Sigma-Aldrich, G9391; ThermoFisher Scientific Cell Culture Petri Dishes ...
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bioRxiv - Immunology 2023Quote: ... then incubated in wash buffer (Advanced DMEM, 10% FBS, 2 mM L-glutamate, penicillin-streptomycin) with 2 mg/mL collagenase type I (Invitrogen) at 37°C for 5-15 mins with vigorous mixing as previously described.57 Crypts were plated in Matrigel with Human Organoid Growth Media (StemCell) ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
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bioRxiv - Bioengineering 2023Quote: HEK293 parental cells and HEK293T cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM (GIBCO) with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were infected with lentiviral vector containing the wild type WWOX and selected with 2 mg/mL G418 (Gibco: 11811031)(36)
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bioRxiv - Microbiology 2023Quote: MA104 cells (ATCC, type: MA104 clone 1, ATCC CRL2378.1, cat. # ATCC CRL2378.1) were grown in M199 medium (Thermo Fisher Scientific) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Microbiology 2023Quote: ... We transfected HEK293T cells with pCAGGS alphavirus E3–E2–(6K/TF)– E1 wild-type or mutant expressor plasmids using Lipofectamine 3000 (Invitrogen) or an empty pCAGGS vector ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were transfected with either wild-type PADI2 (WT) or the PADI2 triple mutant (with L642A/W161A/T159A) using Lipofectamine 3000 (Invitrogen) in 60mm plates ...
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bioRxiv - Plant Biology 2023Quote: ... AHL10 wild type, phosphomimic, or phosphonull cDNA clones (described in Wong et al., 2019) were inserted into pDONR221 P1-P4 (Invitrogen) and the RRP6L1 cDNA was amplified from total mRNA and cloned into pDONR221 P3-P2 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: C6/36 cell lines of Aedes albopictus origin were supplied by the American Type Culture Collection (ATCC) were routinely cultured at 28°C with 5% CO2 in RPMI 1640 medium (Gibco) with GlutaMAXTM Supplement containing 10% FBS.
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bioRxiv - Developmental Biology 2023Quote: Lung tissue was minced with a razor blade and resuspended in a digestion buffer containing of 480 U/ml Collagenase Type I (Gibco), 50 U/ml Dispase (Corning) ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lines (American Type Culture Collections, Manassas, VA) were maintained in DMEM (Macgene Biotech., Beijing) supplemented with 10% FBS (Gibco), 100 U/ml penicillin and 100 mg/ml streptomycin at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: 293T cells were obtained from the American Type Culture Collection (ATCC) and HeLa Flp-In T-Rex cells were obtained from Invitrogen. All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: LS1034 and SW480 cell lines were obtained from the American Type Culture Collection (ATCC) and maintained in RPMI 1640 (Gibco) and L15 medium (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated from in vitro induced MCECs of both wild type and Edf1 knockout at indicated time points using TRIzol Reagent (Invitrogen). 1ug of total RNA was reverse transcribed to form cDNA using the HiScript II Reverse Transcriptase kit (Vazyme ...
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bioRxiv - Cell Biology 2023Quote: H9c2 myoblasts from the American Type Culture Collection (ATCC) were cultured in DMEM medium (Hyclone) supplemented with 10% FBS (Gibco) and 1% penicillin-streptomycin (Hyclone) ...
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bioRxiv - Cell Biology 2023Quote: ... and Huh7 cells were obtained from the American Type Culture Collection (ATCC) and grown in Dulbecco’s modified Eagle’s medium (Life Technologies,11965126) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: The GC cell line MKN-45 cell was purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Life Technologies) with 10% FBS and antibiotics (100 IU/mL penicillin and 100 µg/mL streptomycin) ...
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bioRxiv - Cell Biology 2023Quote: Wild type Homo sapiens telomerase immortalized foreskin fibroblasts (TIFF) were cultured in Dulbecco’s modified Eagle’s medium (DMEM GlutaMAX, ThermoFisher Scientific, #31966047), supplemented with 20% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... Mice in the 22 month age group were wild type and the tissue slices were stained with DAPI (Thermo Fisher), which is a fluorescent nuclear and chromosome counterstain and can emit blue fluorescence.
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bioRxiv - Biochemistry 2024Quote: ... and murine breast cancer cell line 4T1 were acquired from the American Type Culture Collection and cultured in DMEM (Gibco) medium supplemented with 10% FBS and 100 units/ml penicillin-streptomycin (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... the medial samples from each animal cohort were pooled and digested in collagenase type IV (2.5 mg/mL, Life Technologies) and porcine pancreatic elastase (2.5 U/mL ...
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bioRxiv - Molecular Biology 2024Quote: HeLa and MCF7 cell lines obtained from the American Type Culture Collection (ATCC) were cultured in high glucose DMEM (Invitrogen) with 10% FBS (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Analyses were performed on a MALDI-TOF-TOF geometry mass spectrometer (Analyzer 4800 type, Applied Biosystems/MDS Sciex, Toronto, Canada) equipped with a laser source ...
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bioRxiv - Biochemistry 2024Quote: ... followed by an enzyme solution consisting of 8 nM wild-type PETase or its mutant labeled with 0.8 nM Qdot655 (Thermo Scientific), 2 mM 2-Mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: The peptide samples were dissolved in 0.1% formic acid and then analyzed by using two types of mass spectrometers: Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher) for the analysis of protein differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... cells were trypsinized from tissue culture plates or harvested from collagen gels by disassociating the gel with 500 µl of PBS containing 1 U/ml collagenase type I (Gibco). Cells were washed once with PBS ...
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bioRxiv - Cell Biology 2024Quote: Murine cell lines RAW 264.7 and J774A.1 were purchased from the American Type Culture Collection (ATCC, USA) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) media ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were sectioned (10-30 µm thickness) using Kawamoto’s cryofilm type 2C (SECTION-LAB Co. Ltd., Japan) using a cryotome (CryoStar NX70, Thermo Scientific) (59) ...
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bioRxiv - Developmental Biology 2024Quote: Wild-type experiments were performed with the mouse embryonic stem cell line HM1 (Doetschman et al., 1987) (Thermo Fisher, MES4303). The Nfia;Nfib knockout mutant mouse ES cell line (dKO ...
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bioRxiv - Microbiology 2024Quote: ... Skin at infection area was excised and dissociated in 500 µl of enzyme solution containing 1 mg/ml Collagenase Type II (Gibco), 1 mg/ml DNase I (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... biopsies were sectioned into four pieces and subjected to digestion with a 500 µl enzyme solution containing 1 mg/ml Collagenase Type II (Gibco), 1 mg/ml DNase I (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Parental TF-1 cells and transduced cell populations expressing wild-type Hck also require human GM-CSF (1 ng/mL; ThermoFisher) to proliferate and survive in culture ...
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bioRxiv - Microbiology 2024Quote: The Jurkat cell line E6-1 was obtained from the American Type Culture Collection (ATCC) and cultured in RPMI media (Gibco) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2024Quote: HeLa ZAP knockout cells were seeded and transfected either with eGFP-tagged wild-type ZAPS (WT) or eGFP-tagged ZAPS Y659A (Y659A) or eGFP-tagged AID (AID) construct using Lipofectamine 2000 (Invitrogen). At 24 h post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... an aliquot of the clarified supernatant of TDCV was inactivated by UV-C in a Class II type A2 laminar flow biosafety cabinet (Herasafe; Thermofisher) for 30 min before being diluted and applied to a separate 12-well tissue culture plate of DFT2 cells ...
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bioRxiv - Biochemistry 2024Quote: Wild-type B16F10 melanoma cells (ATCC) were cultured in DMEM supplemented with 10% fetal bovine serum (Life Technologies Cat# 26140079), 1% GlutaMAX (Gibco Cat# 35050061) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA from SK-DSRCT2 wild-type cell line was reverse transcribed using SuperScript™ IV Reverse Transcriptase (Thermo Scientific) and used to amplify EWSR1::WT1 mRNA sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting MaPSy minigene input libraries were transfected into HEK293T cells obtained from the American Type Culture Collection in three cell culture replicates using Lipofectamine 3000 (Invitrogen) in a 6-well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... Another digestion was conducted using 1000 U/mL collagenase type 2 and 11 U/mL dispase (Gibco, Cat# 17105-041) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The 3xHA-NTSR1 plasmid consists of N-terminal 3xHA-tagged wild-type human NTSR1 cloned into the pcDNA3.1(+) vector (Invitrogen, Thermo Fisher Scientific Corporation ...
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bioRxiv - Genomics 2024Quote: ... strain Lister 427 wild type strain were grown at 27 °C in SDM-79 (PAA Laboratories) supplemented with heat inactivated 10% FBS (Gibco) and 7 μg.mL-1 hemin (56) ...
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bioRxiv - Biochemistry 2024Quote: ... the entire EGF domain and the flanking region modified by mucin-type O-glycans (amino acid position 18-302) were inserted into the pSeqTag2C-MycHis6 vector (Invitrogen) at the SfiI/NotI sites ...
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bioRxiv - Neuroscience 2024Quote: ... were seeded in 60 mm cell culture dishes and transfected 24h later at a confluence of 50-70% with 6 µg of DraIII-linearized pCMV6-Entry wild-type or Δredox mutant CaMKIIα expression vector using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...