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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Then 20 µg protein per sample was loaded into NuPAGE 4–12% Bis-Tris precast (Invitrogen) gels for electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: DRGs were sectioned at 20μm onto SuperFrost Plus charged slides (Fisher Scientific; Cat 12-550-15). Sections were only briefly thawed in order to adhere to the slide but were immediately returned to the -20°C cryostat chamber until completion of sectioning ...
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bioRxiv - Pathology 2024Quote: ... and Fisherbrand™ RNase-Free Disposable Pellet Pestles (#12-141-364) were purchased from Fisher Scientific. Invitrogen™ RNAlater™ Stabilization Solution (#AM7020 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were kept in differentiation medium (Dulbecco’s Modified Eagle Medium:Nutrient Mixture F-12 [21331-020, Gibco™] containing 1x GlutaMAX [35050-038 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then cultured in non-tissue culture treated flasks (Thermo Scientific, CAT: 12-566-85) with media containing 89% RPMI 1640 ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein were loaded onto a 4 to 12% gradient gel (NuPAGE Invitrogen). Protein was transferred from the gel to 0.2 μm NC membranes at 25 V for 10 min using transfer stacks (iBlot3 Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: Protein samples were separated on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific NP0321, NP0322) using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific NP0001 ...
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bioRxiv - Biochemistry 2024Quote: ... SH-SY5Y and U2OS cells were cultured in DMEM/F-12 (Gibco, Cat No. 32500-043) medium supplemented with 100 U/ml penicillin ...
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bioRxiv - Biophysics 2024Quote: ... The immunocomplexes were removed from the lysate using 12 µL Protein G Dynabeads (Thermo Fisher Scientific), washed four times with ice-cold IP wash buffer (50 mM Tris (pH 7.8) ...
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bioRxiv - Biophysics 2024Quote: ... The immunocomplexes were removed from the lysate using 12 µL Protein G Dynabeads (Thermo Fisher Scientific), washed four times with ice-cold IP wash buffer (50 mM Tris (pH 7.8) ...
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bioRxiv - Bioengineering 2024Quote: ... Labelling of DNA primer was validated by SDS-PAGE with 4-12% Bis-Tris gel (Invitrogen) (Figure S11) ...
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bioRxiv - Bioengineering 2024Quote: ... Denatured proteins were resolved in NuPAGE 4 to 12% bis-tris gradient gels (Thermo Fisher Scientific) and transferred to iBlot2 PVDF ministack membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... 20-60 µg of lysate was loaded on to 4-12% Bis-Tris gels (NuPAGE, Invitrogen) for electrophoresis and immunoblotting.
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bioRxiv - Developmental Biology 2024Quote: ... The 193 cell line was cultured in low calcium medium (DMEM/F-12 1:1 [Gibco, special order medium with no calcium] supplemented with 5% chelated horse serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... The lysate was loaded onto a NuPAGE Novex 4–12% Bis-Tris Protein Gel (Invitrogen, NP0335BOX) and separated in NuPAGE MES SDS running buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: TurboID samples were separated on a 4-12% NuPAGE Bis-Tris precast gel (Thermo Fisher Scientific) for 10 min at 170 V in 1x MOPS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... CD3 expression was detected using PE-CD3 monoclonal antibody (Thermo Fisher Scientific, catalog #12-0038-42). CD19 expression was detected using a FITC Mouse anti-Human CD19 antibody (BD Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... and 44% (v/v) advanced DMEM/F-12 (Ad-DF; Cat#12634-028, Thermo Fisher Scientific) supplemented with 2% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... Equivalent amounts of total proteins were resolved on 4-12% gradient BIS-TRIS gels (Invitrogen #NP0321BOX) under reducing and denaturing conditions ...
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bioRxiv - Cell Biology 2024Quote: ... cell lysates or immunoprecipitated materials were fractionated on 4-12% NuPAGE gels following manufacture’s instruction (ThermoFisher). Proteins were transferred to nitrocellular membranes (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... CU-ACC cells were cultured in 3:1 (v/v) Ham’s F-12 Nutrient Mixture (Gibco #)–DMEM (Gibco # ...
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bioRxiv - Cell Biology 2024Quote: ... cells were maintained at 37C and 5% CO2 in phenol-free DMEM/F-12 media (Gibco) supplemented with 20 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were loaded and run on NuPAGE Novex 4-12% Bis-Tris gels (Thermo Fisher, #WG1403BOX).
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bioRxiv - Cancer Biology 2024Quote: ... Fifteen µg protein was subsequently loaded per well onto NuPage 4-12% Bis-Tris gels (Invitrogen) and then transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2024Quote: SDS-PAGE was conducted utilizing BoltTM 4%–12% Bis-Tris Plus pre-made gels from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and sectioned into 40 µm sections onto Superfrost Plus microscope slides (Fisher Scientific, 12-550-15). Tumor sections were rehydrated in PBS-T and subsequently blocked with 5% normal goat serum (The Jackson Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... and the PC-12 Adh cells were cultivated in F-12K medium ((# 21127022; Thermo Fisher Scientific) with 2.5 % FBS and 15 % horse serum (# 16050122) ...
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bioRxiv - Cancer Biology 2024Quote: ... Between 10-40µg of protein lysate were separated on 4-12% Bis-Tris gels (Life Technologies) and transferred to polyvinylidene fluoride membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... organoids were maintained in complete medium composed of Advanced DMEM/F-12 (Thermo Fisher Scientific #12634010) supplemented with 1X penicillin–streptomycin (Gibco #15140122) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on a NuPAGE Novex 4–12% Bis-Tris Protein Gel (Thermo Fisher Scientific) in NuPAGE™ MOPS SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: Proteins were resolved by molecular weight using pre-cast 4-12% SDS-PAGE gels (Invitrogen, WG1402) run for 4 hours at 160V on ice in standard SDS-MOPS buffer (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... immunoprecipitation eluates were separated on a pre-cast 4−12% NuPAGE gel (Thermo Fisher Scientific NP0321BOX) and protein bands were stained using SimplyBlue SafeStain solution (Thermo Fisher Scientific LC6060) ...
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bioRxiv - Biophysics 2021Quote: ... a double-stranded DNA fragment for the wild-type Gsα short isoform was codon optimized and synthesized using the GeneArt service from Thermofisher. This fragment carried overlapping sequences with the previously described pET15b MBP-Gsα mutant sequence (Huang et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Rat RFL-6 fibroblasts were purchased from the American Type Culture Collection (ATCC) and cultured in Ham’s F-12K medium (Thermo Fisher) supplemented with 20% FBS as previously described (Qiang et al. ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Intestinal pieces were then placed into 10 mL RPMI 20% FCS with 0.5 mg/mL Collagenase Type I (Gibco #17100017) and 0.1 mg/mL DNase I (Sigma #DN25 ...
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bioRxiv - Immunology 2021Quote: Thymi from 4-week-old wild type mice were harvested and 5 million thymocytes per condition were cultured in IMDM medium (Gibco) with 2% FBS (HyClone Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Extracted type VI-B PCR-products were re-amplified using the Maxima Hot Start Taq DNA polymerase (Thermo Fisher Scientific), M13-B185_223bp_C2F and P1-B185_223bp_C2R primers ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Developmental Biology 2020Quote: Wild type or lethally irradiated asexual animals were used as starting material for isolation of total RNA with Trizol Reagent (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... were amplified from genomic DNA by PCR from strains carrying either a wild type copy of csr-1 or csr-1(ADH) and cloned in pENTR-D-TOPO (Invitrogen). Plasmids used for Multisite Gateway reaction are listed as follows ...
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bioRxiv - Molecular Biology 2022Quote: Human cervix carcinoma HeLa and human lung carcinoma A549 cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/ThermoFisher, Paisley, UK) supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumor was dissected from the hind limb and dissociated by shaking for 45 minutes at 37°C in collagenase Type IV (Gibco), dispase (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription for both types of extracted materials was performed with the SuperScript™ VILO™ cDNA Synthesis Kit (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild type and catalytic mutant H672N RNase L plasmids cDNAs (the backbone is pcDNA5-FRT-TO with CMV promoter, Invitrogen) used in this study were a generous gift from Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from adult DRG from 6-month old mutant and wild-type mice (4 per genotype, mixed male and female groups) using the RNaqueous Micro kit (Ambion). All DRGs were pooled for each sample to obtain enough RNA for analysis ...
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bioRxiv - Synthetic Biology 2020Quote: ... were purchased from the American Type Culture Collection and were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher, 11965118) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... Purified lipids were introduced by direct infusion (electrospray ionization-MS) into a trap-type mass spectrometer (LTQ-XL; Thermo Scientific) and identified by comparison with standards ...
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bioRxiv - Microbiology 2021Quote: ... B.1.617.2 Spikes were generated by overlapping PCR using a codon-optimized wild-type SARS-CoV-2 Spike gene (GeneArt, ThermoFisher) that was synthesized (Biobasic ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...