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Citations for Thermo Fisher :
4301 - 4350 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: C2C12 cells from the American Type Culture Collection (ATCC, CRL-1772) were cultured on 10 cm plates (Thermo Scientific, 172931) in 10 mL myoblast growth media ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from pachytene cells taken from wild-type and Tcfl5+/− mice using Trizol reagent (15596018, Life technologies). Two biologic replicates were executed and used for sequencing ...
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bioRxiv - Immunology 2021Quote: from PyMT or wild-type littermate control were cut in small pieces and digested for 1h at 37°C in CO2-independent medium (Gibco) containing 150 μg/mL DNAse and 75 μg/mL Liberase TL (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... full-length wild-type or mutant-coding sequences (CDS) of AtFN3K were cloned into pENTR/D-TOPO Gateway entry vector (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Microbiology 2020Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific). The wild-type SARS-CoV-2 spike (S ...
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bioRxiv - Genomics 2020Quote: ... American Type Cell Culture Collection (ATCC)] were cultured in Dulbecco’s modified Eagles medium plus Glutamax(10566-016, Thermo Fisher Scientific) with 10% (v/v ...
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bioRxiv - Genomics 2020Quote: For each cell type to be made into a library we obtained cell pellets that were stored in RNAlater (Thermofisher) as catalogue items from PromoCell (http://www.promocell.com ...
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bioRxiv - Genomics 2020Quote: ... American Type Cell Culture Collection (ATCC)] were cultured in Dulbecco’s modified Eagles medium plus Glutamax (10566-016, Thermo Fisher Scientific) with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Qβ-VLPs were then re-packaged with B-type 1668 CpGs (5″-TCC ATG ACG TTC CTG ATG CT-3″) with phosphorothioate backbone purchased from (InvitroGen). The re-packaging was confirmed by 1% agarose gel stained with SYBR Safe dye for 30min at 90V ...
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bioRxiv - Biochemistry 2021Quote: Virus stocks were produced by transfecting HEK293T cells with wild-type or mutant pLAI constructs using the Lipofectamine 2000 Transfection Reagent (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... was selected and transcribed in vitro before being transfected into wild-type hTERT RPE-1 cells constitutively expressing Cas9 using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Colon ulcers and distant normal colon tissue were excised from biopsy-wounded wild-type and Vil1-Cre;Rosa26Bmp4 mice at 1 day and 3 days post-wounding and stored in RNAlater (ThermoFisher) at −80°C prior to extraction ...
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Characterization of a novel Fgf10CreERT2 knock-in mouse line targeting postnatal lung Fgf10 lineagesbioRxiv - Developmental Biology 2021Quote: ... Then using sharp blades to cut the lung into small pieces and digested with 0.5% collagenase Type IV in HBSS (Life Technologies, Invitrogen) for 45 minutes at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... excised 4cm2 skin sections were diced into small pieces with a scalpel blade and enzymatically digested with Collagenase type I (500 U/ml; Gibco) and DNAse I (1 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... explants were seeded in 96-well plates coated with poly-L-lysine/collagen type I and neurobasal medium supplemented with of 1X B27 (50X Gibco), 20 μM L-glutamine ...
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bioRxiv - Molecular Biology 2023Quote: ... CNOT8 or CNOT11 constructs were generated in knockout cells (or wild type cells for CNOT8) using the Flp-In system (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were purchased from China Center for Type Culture Collection (Wuhan, China) and were cultured in Dulbecco’s modified Eagle medium (Gibco, USA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: BJ hTERT FAP-mCER-TRF1 wild type (BJ FAP-TRF1 WT) were previously described 16 and were grown in DMEM (Gibco) with 10% Hyclone FBS ...
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bioRxiv - Bioengineering 2023Quote: HEK293 parental cells and HEK293T cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM (GIBCO) with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded at the final density of 2×106 cells/ml in the central chamber of the DAX-1 chips embedded in a matrix composed of type I rat tail collagen at the final concentration of 2.3 mg/ml (Thermofisher, #A1048301). Each chip can be loaded with 10µl of gel ...
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bioRxiv - Biochemistry 2023Quote: ... and human phosphatidylinositol 5-phosphate 4-kinase type-2 beta (hPIP4KB; Uniprot #P78356) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). Gene sequences were subcloned into either bacterial ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Cancer Biology 2023Quote: Freshly FACS-sorted cells were plated into 96 wells at 400,000 cells per well for each cell type and cultured for 24-48 hours in 200 ul of RPMI medium with 10% FCS (Gibco), 2 mM glutamine (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding KNL12026-2342 and ZWINT wild-type and mutants were cloned into a pCDNA5/FRT/TO-EGFP-IRES plasmid and co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from late (passage 4, P4) CNCCs differentiated from hESC wild-type or mutant cell-lines using Trizol reagent (Invitrogen) for at least two differentiations ...
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bioRxiv - Cell Biology 2022Quote: ... Fresh primary mouse hepatocytes were cultured on Type I collagen-coated 60 mm Permanox plates (Thermo Scientific, Grand Island, NY) in William’s E Medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Microbiology 2024Quote: Heat-killed Streptococcus pneumoniae (HK-Spn) was prepared by growing wild-type D39 in Todd-Hewitt Broth (Thermo Scientific, CM0189B) with 2% Yeast Extract (Sigma ...
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bioRxiv - Genetics 2024Quote: ... MDCK cells were transfected with overexpression vectors containing either wild-type or mutant (c.1043C > T) ACTB in Opti-MEM (Gibco) using a NEPA21 electroporator (Nepagene) ...
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bioRxiv - Cell Biology 2024Quote: ... Pooled IVDs were isolated and rendered into a single cell suspension by using four serial digestions of 2mg/mL Collagenase Type II (Gibco) in DMEM at 37 degrees Celsius for 30 minutes each ...
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bioRxiv - Molecular Biology 2024Quote: ... TPL interactor genes were amplified either from yeast gDNA or as cDNAs from wild-type Col-0 RNA using reverse transcriptase (SuperScript IV Reverse Transcriptase, Invitrogen) and gene-specific primers from IDT (Coralville ...
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bioRxiv - Microbiology 2024Quote: ... and HCT116 cell lines were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen 11995-065) containing 25 mM glucose ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were infected with lentiviral vector containing the wild type WWOX and selected with 2 mg/mL G418 (Gibco: 11811031)(36)
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bioRxiv - Microbiology 2023Quote: MA104 cells (ATCC, type: MA104 clone 1, ATCC CRL2378.1, cat. # ATCC CRL2378.1) were grown in M199 medium (Thermo Fisher Scientific) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Microbiology 2023Quote: ... We transfected HEK293T cells with pCAGGS alphavirus E3–E2–(6K/TF)– E1 wild-type or mutant expressor plasmids using Lipofectamine 3000 (Invitrogen) or an empty pCAGGS vector ...
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bioRxiv - Cell Biology 2023Quote: H9c2 myoblasts from the American Type Culture Collection (ATCC) were cultured in DMEM medium (Hyclone) supplemented with 10% FBS (Gibco) and 1% penicillin-streptomycin (Hyclone) ...
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bioRxiv - Cell Biology 2023Quote: HeLa cell lines (American Type Culture Collections, Manassas, VA) were maintained in DMEM (Macgene Biotech., Beijing) supplemented with 10% FBS (Gibco), 100 U/ml penicillin and 100 mg/ml streptomycin at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: 293T cells were obtained from the American Type Culture Collection (ATCC) and HeLa Flp-In T-Rex cells were obtained from Invitrogen. All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: Wild type Homo sapiens telomerase immortalized foreskin fibroblasts (TIFF) were cultured in Dulbecco’s modified Eagle’s medium (DMEM GlutaMAX, ThermoFisher Scientific, #31966047), supplemented with 20% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: Lung tissue was minced with a razor blade and resuspended in a digestion buffer containing of 480 U/ml Collagenase Type I (Gibco), 50 U/ml Dispase (Corning) ...
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bioRxiv - Cell Biology 2023Quote: The GC cell line MKN-45 cell was purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Life Technologies) with 10% FBS and antibiotics (100 IU/mL penicillin and 100 µg/mL streptomycin) ...
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bioRxiv - Genomics 2023Quote: ... or control wild-type tissue were PCR amplified using the diluted protocol of the Phire Animal Tissue Direct PCR Kit (ThermoFisher) and a pair of oligonucleotides (Forward ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were transfected with either wild-type PADI2 (WT) or the PADI2 triple mutant (with L642A/W161A/T159A) using Lipofectamine 3000 (Invitrogen) in 60mm plates ...
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bioRxiv - Plant Biology 2023Quote: ... AHL10 wild type, phosphomimic, or phosphonull cDNA clones (described in Wong et al., 2019) were inserted into pDONR221 P1-P4 (Invitrogen) and the RRP6L1 cDNA was amplified from total mRNA and cloned into pDONR221 P3-P2 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: Lungs were perfused with PBS-EDTA (0.5 mM) and digested in RPMI-1640 medium containing 1 mg/mL collagenase type IV (Life Technologies/ Thermofisher) and 30 µg/mL DNase I (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... and murine neuroblastoma cells N2A (CCL-131) were purchased from the American Type Culture Collection (ATCC) and maintained in DMEM (Gibco) supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin (P/S) ...
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bioRxiv - Microbiology 2023Quote: C6/36 cell lines of Aedes albopictus origin were supplied by the American Type Culture Collection (ATCC) were routinely cultured at 28°C with 5% CO2 in RPMI 1640 medium (Gibco) with GlutaMAXTM Supplement containing 10% FBS.
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bioRxiv - Cancer Biology 2023Quote: LS1034 and SW480 cell lines were obtained from the American Type Culture Collection (ATCC) and maintained in RPMI 1640 (Gibco) and L15 medium (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Mice in the 22 month age group were wild type and the tissue slices were stained with DAPI (Thermo Fisher), which is a fluorescent nuclear and chromosome counterstain and can emit blue fluorescence.