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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Both cell types were re-plated upon reaching 70% confluency onto new cell culture dishes using TrypLE Select Enzyme solution (Gibco).
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bioRxiv - Bioengineering 2020Quote: ... The samples were sectioned using Kawamoto’s cryofilm type 2C (SECTION-LAB Co. Ltd., Japan) to 7 µm thickness using a cryotome (CryoStar NX70, Thermo Scientific) according to the Kawamoto protocol [48] ...
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bioRxiv - Bioengineering 2020Quote: ... wild-type Caco-2 cells were seeded into a 0.1% gelatin coated 150 mm cell culture dish (Fisher Scientific, 12565100) and grown until just fully confluent ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from different types of Lymnaea tissue (CNS, buccal mass, penis, ovotestis, gut and mantle) was purified with TRIzolTM Reagent (ThermoFisher). Reverse transcription (RT ...
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bioRxiv - Cancer Biology 2021Quote: ... These lentiviruses were used to infect ARID1A wild-type BLCa cell lines (T24, 5637 and RT112) which were then selected for stably transfected clones using puromycin (Gibco, ThermoFisher), at the concentration of 1-2µg/ml for 2 weeks ...
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bioRxiv - Cancer Biology 2020Quote: ... BALB/c-derived A20 B lymphoma cell line was obtained from the American Type Culture Collection (Rockville, MD) and maintained in RPMI 1640 supplemented with 10% FCS (GIBCO). For in vivo experiments A20 cell lines were injected i.v ...
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bioRxiv - Cancer Biology 2020Quote: ... After mechanical disruption the tissue is washed in Basis medium (220rcf, 5min) and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and Huh7 cells were obtained from the American Type Culture Collection (ATCC) and grown in Dulbecco’s modified Eagle’s medium (Life Technologies,11965126) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2021Quote: All yeast strains were derived from parental strains Fy251 [American Type Culture Collection (ATCC) 96098] or the two-hybrid strain MaV20330 (Invitrogen). Yeast transformations were carried out using the lithium acetate method48 ...
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bioRxiv - Microbiology 2020Quote: ... aureus Newman wild-type and Newman Δspa were grown in NB treated with 0.5 μg/mL RNase A (Thermo Fisher) at 37°C for 1.5 h prior to sterilization by autoclaving ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative expression of NKS1 was determined in wild type and nks1 alleles by qRT-PCR using SYBR Green (Applied Biosystems) reaction mixture on an ABI PRISM 7900 HT sequence detection systems (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... AT5G02350 and AT2G13900 genes were amplified by PCR from wild-type genomic DNA and cloned into donor vector pENTR/TOPO (Invitrogen). The coding sequences were then transferred to destination vector pGWB2 vector by LR recombination (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted from wild-type Pt1 cells and the gene Phatr3_J39274 was amplified by PCR using the PhusionTM Polymerase (Thermo Fisher) (see table below) ...
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bioRxiv - Microbiology 2021Quote: ... fixed parasites were incubated with 5µg/mL Blood Group Antigen H (O) Type 1 monoclonal antibody (Invitrogen 13-9810-82), washed three times with 1x PBS and incubated with 1:100 α-mouse secondary antibody conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 15 mM HEPES DMEM/F12 no phenol red) were seeded onto plates coated with 50 μg/ml collagen type I from rat tail (ThermoFisher) for 1 to 2h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: MEL and HEK293T cells were purchased from the American Type Culture Collection (Manassas, VA) and cultured in RPMI1640 (A10491-01, Gibco) or DMEM (SH30243.01 ...
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bioRxiv - Cell Biology 2020Quote: ... MCF10A wild-type and LifeAct::RFP mammary epithelial cells (ATCC, CRL-10317) were cultured in DMEM/F12 (Thermo Fisher, 11330057) supplemented with 5% horse serum (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... tumors were minced and digested at 37°C in FBS-free DMEM/F12 Glutamax containing 1mg/ml type IV collagenase (Gibco). Digested tumors were passed through a 100 μm and a 70 μm strainer to isolate organoids of 70-100 μm in size ...
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bioRxiv - Biochemistry 2022Quote: ... was formed in duplex buffer (IDT, 11010301) and electroporated into 2 × 105 wild type K562 cells using the Neon electroporation system (ThermoFisher) at 1450 V ...
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bioRxiv - Neuroscience 2022Quote: ... was generated from 5 µg of total RNA pooled with equivalent amount of all types of olfactory tissue samples using oligo dT-primed cDNA synthesis with Superscript III Reverse Transcriptase (Invitrogen) for the generation of templates for subsequent PCR reactions ...
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bioRxiv - Plant Biology 2022Quote: ... the ORF of PFA-DSP1 (wild-type sequence and with a mutated sequence encoding the C150S substitution) was swapped by LR clonase II (Invitrogen) from pDONR221 into the plant expression vector pGWB641 57 ...
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bioRxiv - Microbiology 2022Quote: ... the IFN-β–Luc was co-transfected with pRL-TK in wild type and 4EHP-KO HEK293 cells using Lipofectamine 2000 according to the manufacturer′s protocol (Invitrogen). 24 h after transfection ...
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bioRxiv - Immunology 2022Quote: ... BEAS-2B bronchial epithelial cells were obtained from the American Type Culture Collection (ATCC CRL-9609) and cultured in DMEM media (Gibco) supplemented with L-glutamine (25030164 ...
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bioRxiv - Molecular Biology 2022Quote: Wild type and MED14-dTAG human colorectal carcinoma HCT-116 cells [25] were grown in RPMI 1640 medium (Gibco, 21875034) supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2020Quote: RNA extraction from 30 zebrafish larvae from the same genotype (homozygous mutant or wild-type) was performed using the TRIzol reagent (Invitrogen) according to the recommended protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301], 100 μL of Collagenase Type I [480U/mL, Gibco, 17100-017] in 9 mL of PBS [Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x 105 ESCs were co-transfected with 1.5 μg wild-type or mutants of PB-ATRX-HA-Neo and 1.5 μg Super PiggyBac transposase using Lipofectamine 3000 (Thermo Fisher). After two days of transfection ...
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bioRxiv - Microbiology 2020Quote: The African green monkey kidney Vero E6 cell line was obtained from the American Type Culture Collection (ATCC, no. 1586) and maintained in Minimum Essential Medium (MEM; Gibco Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: Wild-type and the three LXXAA mutants of TIF2NRID were amine-labelled with a fluorescent probe (Alexa 488 NHS Ester, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: The wild-type (WT) and mutant GLP-1R constructs were integrated into FlpIn-CHO cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Cancer Biology 2020Quote: ... tumors were dissected from the limb and dissociated by shaking for 45 minutes at 37°C in collagenase Type IV (Gibco), dispase (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... we dissected wild-type or acj6 mutant brains expressing HRP-rCD2 by PNs in pre-chilled Schneider’s medium (Thermo Fisher), removed the optic lobes ...
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bioRxiv - Biochemistry 2021Quote: Isolated splenic wild type (WT) and ADAP-deficient T cells were fluorescently labeled with either CFSE or DDAO-SE (both from Invitrogen) and a total of 5×106 cells were injected intravenously into the tail vein of WT recipient mice ...
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bioRxiv - Molecular Biology 2022Quote: Human VAT and SAT biopsies (30-50g) were minced and digested with 3mg/mL type II collagenase solution (ThermoFisher Scientific Inc./Gibco) in HBSS containing calcium chloride ...
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bioRxiv - Cell Biology 2022Quote: Primary mouse pre-osteoblasts were isolated from the calvaria of 3-day-old C57BL/6J mice via sequential digestion with type I collagenase (Gibco) and dispase (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... NCI-H1975 and NCI-H1650 were obtained from the American Type Culture Collection (ATCC) and cultured in RPMI1640 (Life Technologies) containing 10% FBS (PAN Seratech ...
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bioRxiv - Immunology 2022Quote: ... were collected from naive or SNI mice and incubated in a solution of 1 ml of RPMI 1640 medium with 2 mg/ml of collagenase type II (Gibco) for 30 minutes at 37◦C ...
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bioRxiv - Immunology 2022Quote: For LC-MS analysis of wild type ILC2 1.5 µg of peptide for each sample was analysed on a Q-Exactive-HF-X (Thermo Scientific) mass spectrometer coupled with a Dionex Ultimate 3000 RS (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Gels were chopped into smaller pieces to increase their surface area and incubated with 1:1 ratio of collagen to type I collagenase (Gibco) dissolved in PBS at 2 mg/ml ...
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bioRxiv - Microbiology 2020Quote: The commercial immortal mouse macrophage cell line RAW264.7 was obtained from the American Type Culture Collection and was grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Gaithersburg, MD) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Each slice was embedded into 10 μl of rat type I collagen (Roche Diagnosis; diluted at 80% with 1X MEM (GIBCO), 1X GlutaMax (GIBCO ...
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bioRxiv - Genetics 2021Quote: Total RNA was isolated from the eggs at 24 hpo from a wild-type strain (p50T) and three b-4 mutant strains (e36, e37, and No. 903) using TRIzol (Invitrogen). Reverse transcription was then performed using the oligo (dT ...
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bioRxiv - Molecular Biology 2021Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific) (29) ...
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bioRxiv - Developmental Biology 2021Quote: ... the polyacrylamide sheet was washed twice and incubated with a solution of type I collagen (0.2 mg/ml; Gibco BRL) overnight at 4°C.
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bioRxiv - Molecular Biology 2021Quote: ... cells used in this study were purchased from the American Type Culture Collection (ATCC) and grown in RPMI-1640 (Gibco) supplied with 10% FBS (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... We collected approximately 2.5 million cells of each type and purified their total RNA using a GeneJET RNA Purification Kit (Thermo Scientific). We quantified the concentration of each RNA sample using a NanoDrop™ Spectrophotometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... After mechanical disruption the tissue is washed in Basis medium (220rcf, 5min) and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015 ...
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bioRxiv - Developmental Biology 2021Quote: ... Select rhoaa coding sequences were PCR-amplified from wild type or rhoaa-mutant (R17, Δ3) zebrafish cDNAs and cloned into pCRII-TOPO (Invitrogen), according to manufacturer’s specifications ...
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bioRxiv - Biochemistry 2020Quote: ... T-rex 293 cells (approximately 3 × 106 cells per 10-cm dish) were transfected with 5-HT2C wild-type or mutant DNA using Lipofectamine 2000 (Invitrogen) following manufacturer’s protocols ...