Labshake search
Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... the sections were incubated for 2 hours with Alexa Fluor 649 anti-rabbit (1:500, Invitrogen, USA). Afterward ...
-
bioRxiv - Neuroscience 2024Quote: ... and the plasmid piggyBacTransposase (Systems Bioscience) in a ratio of 8:2:1 using lipofectamine 3000 (Invitrogen). Two days after transfection ...
-
bioRxiv - Biophysics 2024Quote: ... 4-(2-hydroxyethyl)-1-piparazineethanesulphonic acid (HEPES) and tris(hydroxymethyl)aminomethane (Tris) were purchased from Fisher Scientific.
-
bioRxiv - Biochemistry 2024Quote: ... was grown to a density of 2 × 106 cells ml−1 in Expi293 expression media (Gibco #A1435101) at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... DNA of PP2Ac and B55 (2:1 ratio) were diluted in Opti-MEM Reduced Serum Medium (ThermoFisher). Similarly ...
-
bioRxiv - Biochemistry 2024Quote: ... the right lungs were immersed in 2 mL RPMI medium with 1 mg/mL collagenase II (Gibco Life Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... The Maxima SYBR Green qPCR master mix kit [1:2] including ROX [0.18µM] (Thermo Fisher Scientific, K0252) was mixed with forward and reverse primers [268 nM] and cDNA [12.5 ng] in a final volume of 14 µL ...
-
bioRxiv - Developmental Biology 2024Quote: ... batches of 4 embryos were digested in 40µl of a solution of 2:1 0.25% Trypsin (Gibco): Accutase (StemCell Technologies ...
-
bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
-
bioRxiv - Microbiology 2020Quote: The plasmid SV-Psi--Env--MLV and L-LUC-SN were co-transfected with or without an envelope glycoprotein plasmid (pHCMV-VSV-G/pSARS-CoV-1/pSARS-CoV-2/pHIV-1 LAI gp160) into HEK293FT cells using Lipofectamine™ 3000 (ThermoFisher, US). Cell supernatants containing viruses were collected after 2 days of transfection ...
-
bioRxiv - Genomics 2020Quote: ... or No Call (numerical representations 0, 1, 2, −1, respectively) using the BRLMM-P algorithm for 493,290 SNPs [25] (Affymetrix Power Tools (APT) Release 1.16.0) ...
-
bioRxiv - Genomics 2020Quote: ... around 100 mg of liquid nitrogen frozen samples were ground with metal beads (2.5 mm diameter) in a Retschmill apparatus (24 hertz for 2×1 min) before re-suspending in 1 mL Trizol reagent (Thermo Fisher Scientific) and incubating at room temperature for 5 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Triton X-100 in 200 μL of PBS for 5 min and subsequently incubated in 100 μL of click reaction buffer (1× Click-iT cell reaction buffer [Thermo Fisher Scientific], 2 mM CuSO4, 1× Click-iT cell buffer additive [Thermo Fisher Scientific] ...
-
bioRxiv - Biochemistry 2021Quote: ... equal numbers of THP-1 monocytes (2*105 ml−1) were seeded in RPMI 1640 without Lysine and Arginine (Thermo Fisher Scientific), supplemented either with natural amino acids (L-Lysine ...
-
bioRxiv - Cancer Biology 2021Quote: Single live cell suspensions were washed with 1% FCS in PBS and resuspended in flow cytometry buffer (2% FCS and 1 mM ethylenediaminetetraacetic acid (EDTA; Thermo Fisher #AM9260G) in PBS).
-
bioRxiv - Developmental Biology 2021Quote: ... The colonies were lifted with a 1:1 Dispase/Collagenase enzyme mix (1mg/ml; Gibco; 17105-041 and 2 mg/ml; Gibco; 17104-019). and resuspended in 10 ml of Phase 1 medium (1:1 IMDM (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... iAstrocyte differentiation was initiated by switching the medium to astrocyte medium (DMEM/F12+GlutaMAX, 1% N-2 supplement, 1% fetal bovine serum, all from Thermo Fisher Scientific) as previously described [81] and continued for at least 60 days ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... Human neuronal BE(2)-M17 cells (ATCC® CRL-2267) were maintained in a 1:1 ratio of F12 Hams and OptiMEM (GIBCO, USA) media supplemented with 10% horse serum (GIBCO ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with TBS for 10 min and then then nuclei were counterstained by incubation with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from a 1 mg/ml stock solution, Thermo Fisher Scientific #62248) for 5 min ...
-
bioRxiv - Neuroscience 2024Quote: ... Accutase was removed and the cells were then resuspended in DMEM with 2% B-27-supplemented 1% FBS and 1% PSF (Thermo Fisher Scientific) and plated on PDL/Laminin-2020-coated culture plates.
-
bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
-
bioRxiv - Cell Biology 2023Quote: ... and the nucleus was stained with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from 1 mg/ml stock solution (Thermo Fisher Scientific #62248)) ...
-
bioRxiv - Microbiology 2023Quote: ... cells with either MX2-T2A-TurboID or MX2-MUTT2A-TurboID lentivirus and selecting with 2 µg ml−1 puromycin (#ant-pr-1, Thermo Fisher Scientific) for 7 days ...
-
bioRxiv - Microbiology 2023Quote: Pseudotyped viruses were titrated by serially diluting filtered pseudotypes with fresh DMEM starting from a 1:2 to a final 1:512 dilution using white 96-well flat bottom plates (ThermoFisher, Woolwich, UK). Then appropriate target cells were added (Table 1 ...
-
bioRxiv - Genetics 2023Quote: ... The solution was then exchanged with primary antibody diluted in a staining solution (1% DMSO, 0.5% Triton X-100, and 2% NDS in PBS): Sox2 (1:200; Invitrogen, 14-9811-82) or AF647-conjugated β-catenin (1:200 ...
-
bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
-
bioRxiv - Cell Biology 2023Quote: ... and fibroblasts were cultured for 2 days in DMEM/F12 (1/1) supplemented with 10 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France) and 1% Penicillin/Streptomycin (Sigma) ...
-
bioRxiv - Microbiology 2023Quote: SARS-CoV-2 Nucleocapsid antibody (produced in mouse) as a primary antibody was procured from (MA5-35943, Invitrogen, 1:1000-1:10,000), Rabbit phospho-p38 MAPK and total p38 MAPK Antibody were procured from (9211S and 9212S ...
-
bioRxiv - Molecular Biology 2023Quote: ... The iPSCs were then cultured for 7 days in Neurobasal/DMEM-F12 medium (1:1 v/v) containing 2% B27 (Gibco, 17054–044), 1% N2 (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS (1X) and incubated for 2 hours at 37°C with secondary detection antibodies (1:500) and phalloidin (1: 3000) (Invitrogen, Waltham, MA) for cytoskeleton staining ...
-
bioRxiv - Microbiology 2023Quote: ... mosquito bodies and legs were collected and placed in homogenizer tubes with a homogenizer bead and 500μl mosquito homogenization media (DMEM containing 2% FBS, 1% PenStrep, and 1% Fungizone (Gibco, Grand Island, NY)) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Coverslips were labeled with 1 μg/mL mAb 13-2 (rOmpB) (53) and 1 μg/mL rabbit polyclonal αTGN46 (Invitrogen, PA5-23068) in PBS overnight at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg) was injected onto a Dionex UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific) with an additional RSLCnano pump run in online two-dimensional active modulation mode coupled to a Thermo Fusion Orbitrap mass spectrometer.
-
bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated at 37°C for 1 hr under 200 rpm with 2 mg/mL collagenase III (StemCell, #07422) in a complete medium consisting of Hyclone DMEM/F/12 1:1 (Thermo Scientific, #SH30023.01) supplemented with 15mM HEPES ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10 ml of collagenase mix digestion solution (collagenase II + IV mixed at 1:1 ratio at 2 mg/ml -Thermo Fisher Scientific) was added to the tissue and incubated horizontally for 20 min at 37°C in a shaking incubator ...
-
bioRxiv - Bioengineering 2024Quote: ... NK cells and target cells were mixed at an effector to target (E/T) ratio of 4:1 (SK-BR-3 cells) or 2:1 (K562 cells) and Sytox Green (Thermo Fisher Scientific) was added at 100 nM ...
-
bioRxiv - Cell Biology 2024Quote: ... Then CB were diluted 1:1 (v/v) into a dilution buffer (1mM EDTA in PBS supplemented by 2% FBS (Thermo Fisher, #10099141)) and undelayed with density gradient medium LymphoPrep (Stemcell Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... All cultures received N2 medium from d0 to 11 (50% MACS Neuromedium, 50% DMEM/F12 containing 1% N-2 supplement [Thermo Fisher Scientific, #17502048] 1% GlutaMAX [Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... either untreated or treated with the cytokines listed above were diluted either 1:2 or 1:100 and ran using a Luminex Flexmap Assay (ThermoFisher, Lot# L152023) to probe for IL-6 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunostaining were anti-tyrosinated α-tubulin (YL1/2) (1/25 ECACC) and anti-rat Alexa Fluor 555 secondary antibody (1/400, Life Technologies). Phalloidin conjugated with and Alexa Fluor 647 A22287(1/200 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were passaged every 2-3 days with 1:4-1:6 ratio by incubation cells with 0.5 mM EDTA (Fisher Scientific MT-46034CI) for 5 min ...
-
bioRxiv - Bioengineering 2020Quote: ... Bone marrow human macrophage (KG-1 ATCC® CCL246™) passages 2–5 were cultured in cell suspension flasks using IMDM medium (Gibco™ GlutaMAX™, HEPES) with 20% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... each well was washed twice in ice-cold PBS and the cells were scraped off in ice-cold PBS with added protease inhibitors (1:100 Halt, cat nb. 78429, Thermofisher; 5 mM NAM; 2 mM NaOV). The suspension was transferred to a tube and centrifuged for 10 min at 2000g at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... before they were transferred into 2° antibodies dilute (1:700 of Dylight-/Alexa-conjugated antibodies made in donkey purchased from Thermo Fisher Scientific in 5% BSA/PBST) for 2 hrs at RT ...
-
bioRxiv - Immunology 2022Quote: ... Harvested naïve CD4+ T cells were plated at a density of 1.5 × 105 cells/mL in 2 ml of complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies], and 0.05% (50μM) 2-ME [Sigma-Aldrich]) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 0.1 mg/ml dihydro-oxbenzoic acid (Thermo Fisher) and 0.4 mg/ml tyrosine (sodium salt ...
-
bioRxiv - Neuroscience 2023Quote: ... Each individual organoid was incubated in 1.5 mL of Trypsin/Accutase (1:1) containing 1 µL/mL of TURBO™ DNase (Thermo Fisher Scientific, AM2238, 2 U/µL) in a gentleMACS Dissociator (Miltenyi Biotec ...