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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... samples were washed in block six times for 1 hour and incubated in secondary antibodies conjugated to fluorescent dyes diluted 1:250 in block with added 4’,6-diamidino-2-phenylindole (DAPI; 1:500 dilution of 1 mg/ml stock, Thermo Fisher) for 2 nights at 4°C.
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated in the dark at room temperature for 2 hours with 1:1000 DAPI and 1:500 AlexaFluor secondary antibodies (Thermo Fisher) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2-hour incubation with secondary antibody (1:500 in 1% BSA in PBST; AF488 anti-rat, Invitrogen, A-11006) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: The datasets of ULK1C (2:1:1) core and the ULK1C:PI3KC3-C1 mixture were recorded at a 300 kV Titan Krios microscope (Thermo Fisher Scientific) equipped with X-FEG and energy filter set to a width of 20 eV ...
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bioRxiv - Biochemistry 2023Quote: ... fresh P3 virus from γ-TuRC-GFP and γ-TuSC were mixed in a 1:1 ratio and used to infect 1.8-2 L of High five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 30 mL of the γ-TuRC-GFP and γ-TuSC virus mixture as above ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
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bioRxiv - Cell Biology 2022Quote: ... 2 ml of 56°C warm gel solution (1 volume RPMI/20% FBS, 1 volume 2x HBSS, 2 volumes 2.5% agarose in water (ultrapure agarose, Invitrogen, #16500-100)) were cast per 35 mm dish and allowed to solidify ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was then incubated for 1 hr at RT with 2° anti-rabbit HRP antibody (1:10,000, ThermoFisher, Cat #31458). The membrane was washed 5 times 5 min in TBS-T prior to visualization with SuperSignal West Pico Chemiluminescent Substrate (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 1 × 1 cm of mouse skin encompassing the wound site was excised into 2 mL of RNAlater stabilization solution (Invitrogen, USA) and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Neuroscience 2024Quote: Rhod2-AM (Fig. 2 and Fig. S2) or Oregon Green 488 BAPTA-1-AM (OGB-1; other figures; all ThermoFisher Scientific) were bolus-loaded as described 55 with minor modifications ...
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bioRxiv - Cell Biology 2024Quote: ... were added at a dilution of 1:500 in blocking solution (Aligent Antibody Diluent #S202230-2) containing 1:100 Phalloidin 594 (ThermoFisher #A12381) to cells and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed with PBS and starved with smooth muscle cell growth medium 2 containing 1% FBS + 1% insulin-transferrin-selenium (Gibco, 41400045) + 1% P/S overnight ...
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bioRxiv - Immunology 2024Quote: ... or AG1478 (500 nM) for 72 hours in smooth muscle cell growth medium 2 containing 1% FBS + 1% insulin-transferrin-selenium (Gibco, 41400045) + 1% P/S ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were incubated with fluorophore- conjugated secondary antibody in 2% milk in PBS with 0.3% triton X-100 for 1 hour (1:200, Alexa 555, Invitrogen A-21424) then mounted with DAPI-containing Fluoromount medium (Southern Biotech 0100-20) ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.1 M PB and stained with Alexa Fluor (AF) 647-conjugated streptavidin (1:300 diluted from 2 mg ml−1 stock, Invitrogen S32357) overnight in blocking/permeabilising solution ...
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bioRxiv - Cell Biology 2024Quote: ... washed 2 x 2 min with 1X PBS, and incubated with anti-rat 546 (1:500, Thermo Fischer) and DAPI (1:10000, Life Technologies) for 30 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific) and 2× Laemilli Buffer (Biorad) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 1 mL of Krebs solution with calcium containing 1 μM of the fluorescent mitochondrial indicator Rhod-2 AM (Invitrogen, Switzerland) for 1 h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... The culture media for CD34+ cell support following isolation contained DMEM and F12 (2:1 ratio) supplemented with 1% antibiotic/antimycotic (ThermoFisher Scientific), 20 mg/mL ascorbic acid ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovaries from ∼2-5 flies were dissected in Schneider’s Drosophila media (ThermoFisher SKU #21720-001) that was also used for ex vivo culturing ...
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and loaded with 5 μM Rhod-2 AM (R1244, Invitrogen) in HBSS without calcium/magnesium for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached after 2 or 5 days of co-culture with StemPro Accutase (Thermofisher), experimental duplicates were performed for each timepoint and each condition ...
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bioRxiv - Biochemistry 2021Quote: ... Trimer was reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Thermo Scientific) and alkylated with 10 mM 2-chloroacetamide (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... an EdU (5-ethynyl-2′-deoxyuridine) proliferation assay was performed as recommended by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Fibers were incubated in 5 µM rhod-2 in the membrane permeable AM form (Invitrogen). Rhod-2 was excited with 531 nm light and the emitted light was collected through a 585 nm long-pass filter.
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bioRxiv - Molecular Biology 2022Quote: ... 5 x 107 ESCs were fixed with 2 mM DSG (disuccinimidyl glutarate, Thermo Fisher Scientific) for 50 min at 25⁰C and then 1% formaldehyde (methanol-free ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Microbiology 2022Quote: Larvae were immersed in 100 μg/mL 5-ethynyl-2′-deoxyuridine (EdU) solution (A10044; Invitrogen) for 16 h before termination of the experiment ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were counterstained by Hoechst 33342 (Thermo Fisher Scientific, 2 μg/mL, 5 min, RT), and slides were mounted in ProLong Gold antifade mountant (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of proteins was achieved with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and samples were subsequently alkylated in 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, E10187) before and after the session ...
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bioRxiv - Neuroscience 2022Quote: Mice were injected with 100 mg/kg of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen #E10187) by intraperitoneal injection for 5 consecutive days ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with a mix of 5 µM Fura-2 AM (Invitrogen™, F1221) and 0.025% Pluronic® F-127 (diluted in CM ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µL of DNase TURBO 10X Buffer and 2 µL of DNase TURBO (Invitrogen, #AM2238) were then added and the reactions are incubated at 37 °C for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM Hepes-K+ pH 7.6) containing 2 mg/mL of collagenase type II (GIBCO) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 2-5 μl sample were loaded onto 10-20% SDS-tricine gels (Invitrogen, #EC66252BOX) and LPS was silver stained as described previously [47] ...