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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Reduction of proteins was achieved with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and samples were subsequently alkylated in 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were counterstained by Hoechst 33342 (Thermo Fisher Scientific, 2 μg/mL, 5 min, RT), and slides were mounted in ProLong Gold antifade mountant (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μM purified DNMT3A2 was incubated in triplicate with 5× SYPRO Orange (Thermo Fisher Scientific) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... 5-ethynyl-2’-deoxyuridine (EdU, 12.5 mg/kg, Thermo Fisher Scientific Inc. Waltham, MA, USA) was intraperitoneally injected into pregnant C57BL/6 mice once a day (12 P.M. ...
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bioRxiv - Microbiology 2023Quote: ... and 2-5 μl sample were loaded onto 10-20% SDS-tricine gels (Invitrogen, #EC66252BOX) and LPS was silver stained as described previously [47] ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µL of DNase TURBO 10X Buffer and 2 µL of DNase TURBO (Invitrogen, #AM2238) were then added and the reactions are incubated at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 12.5 mM Glucose and 10 μM 5’-fluoro-2’-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells ...
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bioRxiv - Immunology 2024Quote: ... senescent CD4+ T cells were treated with BrdU (5-bromo-2′-deoxyuridine; 10µM; B23151-ThermoFisher) for 72 hours ...
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bioRxiv - Neuroscience 2024Quote: ... then an IP injection of 50 mg/kg 5-ethynyl-2’-deoxyuridine (EdU (ThermoFisher Scientific)) 24 h later and were perfused after a further six days.
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bioRxiv - Immunology 2024Quote: ... approximately 2-5×106 cells were incubated in 40 μl of fixable viability dye (Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was performed with 5 µg of anti-BRN1/2 (PA5-30124 Thermo Fisher Scientific) or rabbit IgG as a negative control ...
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bioRxiv - Molecular Biology 2024Quote: ... UHRR or HEK-293T RNAs (5 μg) were DNased with 2 U TURBO DNase (Invitrogen) and purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Neuroscience 2024Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5-ethynyl-2’-deoxyuridine; Invitrogen, E10187) before the session ...
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bioRxiv - Biophysics 2021Quote: ... 5 % CO2 incubator for 5 hours before replacing the culture media with 2 mL B-DMEM (Thermofisher, cat. # 10566016) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were rinsed 2 times for 5 minutes with PBS++ and incubated with 5 drops of NucBlue (Life Technologies) for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... A single bolus of 50μL 0.5% Texas-red dextran solution (70,000 MW, 5 mg/mL in 0.9% NaCl, t1/2 ∼ 25min, Termo Fisher Scientific) was injected ...
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bioRxiv - Neuroscience 2023Quote: ... A 50μl bolus of 0.5% Texas-red dextran solution (70,000 MW, 5 mg/mL in 0.9% NaCl, t1/2 ∼ 25min, Termo Fisher Scientific) was injected at a constant rate of 30μl/sec using a syringe infusing pump (GenieTouch ...
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bioRxiv - Cell Biology 2023Quote: ... for 2–5 h at 37°C followed by a 5 min incubation in TrypLE express (Thermo Fisher Scientific) to generate small clumps of corneal endothelial cells ...
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bioRxiv - Pathology 2024Quote: ... according to manufacturer specifications in the presence of 10mM 5-Bromo-2’-Deoxyuridine 5’-Triphosphate (BrdUTP, Thermo Scientific, B21550), at 37°C in a dark humidified slide box for 90 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Scientific, 22980) were added to the alginate solution in a molar ratio of 1 alginate:30 NHS:25 EDC ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were then washed with PBT for 2 h and stored in 2 % DABCO (Acros Organics, AC112471000).
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bioRxiv - Neuroscience 2021Quote: The cultured cells were incubated with Fura-2-acetoxymethyl ester (Fura-2-AM; Molecular Probes; 2.5 µM) in MEM (0.7 mL ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from the SARS-CoV-2 infected Caco-2 cells using Trizol (Thermo Fisher). The extracted RNA was then reverse transcribed with the reverse primers specific to Nsp12 ...
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bioRxiv - Microbiology 2021Quote: ... LNPs were stained with 2% uranyl acetate for 2 mins and imaged by Talos F200X (Thermo Fisher) at an accelerating voltage of 200 kV ...
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bioRxiv - Bioengineering 2022Quote: RPTEC organoids were imaged every 2-3 days with a EVOS FL 2 Auto microscope (Thermo Fisher) with a 4x objective ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µl cDNA was combined with 25 µl 2× TaqMan Gene Expression Master Mix (Thermo Fisher Scientific), 2.5 µl of 20× TaqMan Gene Expression Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ACE-2 protein (hsACE-2, residues S19 to D615) was transiently transfected into HEK293E cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Biochemistry 2022Quote: ... The peptide sample was loaded onto the precolumn (75 μm×2 cm, C18, 2 μm, Thermo Fisher) at a flow rate of 400 nL/min and then separated using a reversed-phase analysis column (50 μm×15 cm ...
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bioRxiv - Cell Biology 2022Quote: ... in HBSS (Hanks Balance Salt Solution) without Ca+2 and Mg+2 (Thermo Fisher Scientific, Waltham, MA.) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Transferences were done in an iBlot 2 gel transfer device using iBlot 2 transfer nitrocellulose stacks (Invitrogen). Membranes were blotted against phospho-AKT (Ser473 ...
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bioRxiv - Microbiology 2023Quote: 2 x104 MPI-2 cells/well were seeded in a white clear-bottom 96 well plates (ThermoFisher) and incubated at 37°C with 5% CO2 for 24 hours ...
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bioRxiv - Microbiology 2023Quote: 2 x104 MPI-2 cells/well were seeded in a flat clear-bottom 96 well plates (ThermoFisher) and incubated at 37°C with 5% CO2 for 24 hours before the treatment with 15 nmol of either sfGFP or LysBFL ...
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bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
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bioRxiv - Genomics 2024Quote: ... each with 2 µg PCR input and 2 µL FastDigest® LguI (Thermo Fisher Scientific, Waltham, MA). These reactions were then incubated at 37°C for two hours ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 of the remaining cells were stimulated with 2×106 anti-CD3/CD28 beads (Gibco, 11453D) in 5 ml of IMDM medium supplemented with 10 % FBS ...
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bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
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bioRxiv - Cancer Biology 2024Quote: ... OPM-2 cells were labelled with 2 μg/mL of Vybrant DiD cell-labeling solution (Life Technologies). The labeled cells were suspended in RPMI containing 2 mM EDTA ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were resuspended in a solution of cold PBS with 2% FCS and 2 mM EDTA (ThermoFisher), and either used for experiments with complete medium without 2-mercaptoethanol or frozen in 9:1 FCS/DMSO until use.
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bioRxiv - Neuroscience 2024Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/mL; Molecular Probes) for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... for 2 h and counterstaining with 2 μg/ml Hoechst 33342 (H1399, Thermo Fisher Scientific, MA, USA) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... for 2 hours and counterstaining with 2 μg/mL Hoechst 33342 (H1399, Thermo Fisher Scientific, MA, USA) for 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... and 55 μM 2-Mercaptoethanol (0.1% of 55 mM 2-Mercaptoethanol in DPBS) (#21985023, Thermo Fisher Scientific) in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used were F(ab’)2 goat anti-rabbit Alexa Fluor 568 (Molecular Probes, 1:1000), goat anti-mouse Alexa Fluor 488 (Molecular Probes 1:1000).
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bioRxiv - Immunology 2021Quote: ... T-cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of PBMC-derived CECs (ratio 1:2 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 hours in Alexa 647-conjugated donkey anti-rabbit (1:2000; ThermoFisher), 3 times washed in PBS and mounted by Vectashield (Vector Laboratories ...