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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Spheroids were then embedded in rat-tail collagen (3 mg/ml Life Technologies). Spheroids were then cultured with unconditioned media ...
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bioRxiv - Cancer Biology 2023Quote: ... Rat-derived fibroblast lines were grown in DMEM/F12 no phenol red (Gibco), 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibodies and their sources were: HRP-anti-rat IgG (Invitrogen, 31470), HRP-anti-mouse IgG (Dako ...
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bioRxiv - Immunology 2023Quote: ... Lin+ cells were depleted using sheep anti-rat IgG immunomagnetic beads (Dynabeads, Invitrogen). 8-10 x 106 Lin-depleted BM cells from CD45.1 congenic mice were retro-orbitally injected into 6-8-week-old lethally irradiated (1200 rads ...
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bioRxiv - Evolutionary Biology 2023Quote: ... followed by two secondary antibodies: goat anti-rat Alexa Fluor® 594 (Invitrogen) and goat anti-rabbit Alexa Fluor® 488 (Invitrogen) ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and donkey anti-rat IgG (H+L) Alexa Fluor™ 488 (Thermo Fisher Scientific Cat# A-21208 ...
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bioRxiv - Bioengineering 2023Quote: ... 330 µl of rat tail type-I collagen (3 mg/ml; Gibco A1048301) was gently diluted in 660 µl 1X PBS for a final collagen concentration of 1 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-rat CD73 (Fisher Scientific, BDB551123, 5 μl per 10^7 cells) and mouse IgG microbeads (Miltenyi Biotech ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-B-lymphocyte activation antigen B7-2 (CD86, Invitrogen, MA1-10299, RRID:AB_11152324), guinea pig anti-vesicular glutamate transporter 1 (vGlut1 ...
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bioRxiv - Developmental Biology 2024Quote: ... AlexaFluor555 (anti-mouse) and AlexaFluor488 (anti-rat, both secondary antibodies were from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were washed three times in 1xTBS and incubated in fluorescent Alexa-Fluor-labelled IgG secondary antibodies (Alexa-Fluor goat anti-rabbit 488 Invitrogen A-11008, goat anti-rat 546 Invitrogen A-11081 all 1:400 dilution) in 10% serum solution in 2% TBS-T for 2 h ...
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bioRxiv - Plant Biology 2019Quote: ... the FP coding sequences were each recombined into pMDC32 35S expression vectors17 via the LR Clonase reaction (Invitrogen, Carlsbad, CA). FPs were subsequently re-sequenced prior to transformation into Agrobacterium tumefaciens strain LBA4404.
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bioRxiv - Cell Biology 2020Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, ThermoFisher). For each analysis ...
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bioRxiv - Cell Biology 2019Quote: RNAi experiments were performed by knocking down the gene targets in HeLa SS6 cells growing in 35 mm cell culture plates with two rounds of siRNA transfections using Oligofectamine (Invitrogen) as described (Motley et al. ...
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bioRxiv - Biophysics 2020Quote: The GV_AC28 cells incubated with Dox for over 3 days were plated onto a 35 mm glass bottom dish filled with phenol red-free DMEM (Gibco) with supplements ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons were loaded for 35 minutes at RT with a calcium probe Fluo-4 and pluronic F 127 (v/v; 50%; Invitrogen) diluted to 1/500 in a glutamate-free recording medium (129 mM NaCl ...
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bioRxiv - Genomics 2020Quote: aiFACS-sorted neurons were plated on ornithine-coated glass coverslips (35 mm diameter) and cultivated in complete medium: Neurobasal (Invitrogen) supplemented with B-27 (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... Sucrose gradients were centrifuged at 104,813 g for 35 min at 20°C in a M12EX (Thermo Scientific, Waltham MA) centrifuge with a RP55S rotor (Thermo Scientific ...
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bioRxiv - Immunology 2019Quote: ... and eluted using a 500 μl/min flow rate over 10 min from 2 % buffer A to 35 % buffer B (0.1% formic acid in acetonitrile) with an Ultimate 3000 HPLC system (Thermo Scientific). One ml fractions were collected from 2 to 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... NAA50 was cloned into the cauliflower mosaic virus 35S promoter vector pEarleyGate100 (Earley et al. 2006) using a modified multisite Gateway recombination cloning system (Invitrogen) as described in (Qi et al ...
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bioRxiv - Immunology 2019Quote: ... DRG neurons were plated on laminin pre-coated 35 mm culture dishes (2,000 cells per dish) in 50 ng/ml nerve growth factor/neurobasal media (Thermo Fisher), and used for calcium imaging 12-24 hr after plating ...
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bioRxiv - Neuroscience 2019Quote: ... Striatal neuronal cells seeded in 35 mm glass bottom dishes or other plates were transfected 24 h later with cDNA constructs using lipofectamine 2000 (Invitrogen) in the ratio of 1:2 (DNA ...
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bioRxiv - Biochemistry 2019Quote: ... the cells were prepared for imaging by gently resuspending the cells and transferring them to a 35 mm Nunc glass bottom tissue culture dish (ThermoFisher) where they were allowed to adhere for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... on a semi- permeable (Millicell) membrane in a 35 mm culture dishes (Nunclon) containing 1.2 mL Neurobasal-A (Life Technologies) with 2% B27 (Gibco) ...
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bioRxiv - Plant Biology 2021Quote: ... ROMT2 and ROMT3 were transferred into the binary destination vector pB2GW7 including Cauliflower mosaic virus (CaMV) 35S promoter through Gateway LR clonase recombination (Invitrogen). Together with ROMT1 and STS48 constructs (Schmidlin et al. ...
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bioRxiv - Plant Biology 2021Quote: ... and verified by Sanger sequencing before recombining into a modified pMDC32 vector (Curtis and Grossniklaus, 2003) lacking the 35S promoter using LR Clonase (Invitrogen) (Hanikenne et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Each gene version was cloned into the Gateway transfer vector pDONR221 and then into the binary vector pK7WG2D under the control of the CaMV 35S promoter (Gateway BP and LR Clonase II Enzyme mix, Invitrogen). The resulting plasmids were introduced into the Agrobacterium strain AGL0 following the protocol of (Höfgen and Willmitzer ...
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bioRxiv - Cell Biology 2022Quote: ... Plat-E cells were plated at 1.5×106 cells per 35-mm well and transfected the next day with 3.5 μg plasmid DNA using Lipofectamine 2000 (Thermo Fisher) in DMEM-containing medium according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The 100 μm capillary column was packed in-house with 35 cm of Accucore 50 resin (2.6 μm, 150Å; ThermoFisher Scientific). The mobile phase was 5% acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... 10% spent medium from the renal carcinoma cell line 5637 (ACC-35, DSMZ) and 100 U/ml penicillin-streptomycin (Gibco). Cells were routinely cultured at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: Differentiation day 35 neurons from four GBA and four control iPSC lines were collected on ice in phosphate-buffered saline (PBS, ThermoFisher) with protease-(Complete tablets ...
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Sequence and structural variations determining the recruitment of WNK kinases to the KLHL3 E3 ligasebioRxiv - Molecular Biology 2020Quote: ... Peptides were separated in a 60 min gradient from 2% ACN/5% DMSO in 0.1% Formic Acid to 35% CAN in the same buffer on a 50 cm Easy-Spray Column (Thermo Fisher). MS spectra were acquired in the ion trap with a resolution of 120,000 and with an ion target of 4e5 ...
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bioRxiv - Plant Biology 2020Quote: ... The amiRAHL20 precursor was recombined into destination vectors pMDC32 64 downstream of the 35S promoter via an LR reaction (Gateway, Invitrogen). To generate the pAHL15:AHL15-ΔG construct ...
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bioRxiv - Cancer Biology 2019Quote: ∼1 x 106 HEK293 cells grown at 70% confluency on 35 mm dish embedded with 12 mm glass viewing area (Nunc, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2020Quote: D2.0R (parental, EGFP-shCtrl and EGFP-shEphB6 #31 and #35) cells were transfected with Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000001) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Human induced pluripotent stem cells (hiPSCs) were generated from a healthy 35-year-old male subject using an episomal reprogramming kit (Invitrogen). H9 ESCs were purchased from Wicell ...
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bioRxiv - Plant Biology 2021Quote: ... The fragment was inserted into the pMDC32 binary vector (Curtis and Grossniklaus, 2003) under control of the 35S promoter by Gateway LR cloning (Invitrogen). For generation of the RNAi knockdown plants ...
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bioRxiv - Biophysics 2019Quote: ... Cells grew on the coverslip or the 35 mm cell culture dish in growth medium (DMEM [Gibco; #11880-02] containing 1x MEM NEAA [Cat#11140-035; Gibco] ...
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bioRxiv - Neuroscience 2021Quote: ... at 4°C prior to washing and incubation with secondary antibodies (anti-rabbit AlexaFluor-546 [A11-35] and anti-mouse AlexaFluor-633 [A21052] Thermofisher) in 1 % BSA/PBS ...
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bioRxiv - Microbiology 2020Quote: An initial inoculum of 104 cells/mL in Sabouraud medium was added to 35 mm glass bottom dishes (Thermo Scientific ™ Nunc Glass Bottom Dish ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Human lung fibroblasts were transfected with the indicated siRNA at a final concentration of 35 nM using Lipofectamine RNAiMAX reagent (Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... FAPS were resuspended at 2 X 107 cells/mL and plated in 10 µL high-density micromass dots onto 35 mm tissue culture dishes (Nunc) in DMEM/F12 media (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... RNA was transcribed into cDNA and sequencing libraries were generated as detailed described 35 and were sequenced using the Ion Torrent S5XL Instrument (ThermoFisher). Samples with Ct values >20 for SARS-CoV-2 were additionally treated with RNA baits (myBaits ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were frozen in TissueTek and sectioned at 35 μm thickness on a cryostat (Thermo Scientific Microm HM 550) at −20°C ...
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bioRxiv - Neuroscience 2021Quote: ... the transfected cells were plated on poly-L-lysine-coated 35-mm round coverslip (#1.5 thickness) (Fisher Scientific, Waltham, MA). The cells were used for TIRF imaging 2 days after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... The peptide separation was performed at 35 °C using a C18 column (EASY-Spray column, 15cm x 75 µm ID, PepMap C18, 3µm, Thermo Scientific) at a flow rate of 300 nL/min ...
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bioRxiv - Immunology 2022Quote: ... and LysM-Cre- Ern1fl/fl (ref. (35)) mice were isolated and maintained in RPMI 1640 supplemented with 10% FBS (Gibco), 30% L929 cell supernatant ...
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bioRxiv - Plant Biology 2022Quote: The CDS of ZmEREB57 was inserted downstream of the CaMV 35S promoter in the pcambia1300 vector by means of a Gateway LR reaction (Invitrogen, the gene-specific primers listed in Supplemental Table S8) ...
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bioRxiv - Plant Biology 2022Quote: ... each of the synthetic RNAi fragments was placed under the control of the CaMV-35S promoter in the binary destination vector pK2GW7 using Gateway LR Clonase II kit (Invitrogen). PhnsLTP1-RNAi construct was subcloned into a modified pRNA69 vector containing the Clarkia breweri linalool synthase (LIS ...
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bioRxiv - Plant Biology 2022Quote: ... MMV N and P were amplified using MMV NF/NR and PF/PR primers from cDNAs synthesized from infected-plant RNA and subcloned into pTF binary plasmid between the double CaMV 35S promoter and nopaline synthase (NOS) terminator using Gateway Clonase enzyme mix (Invitrogen) (Figure 1a) ...