Labshake search
Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibodies (1:500 Goat anti -rabbit Alexa Fluor 647 [Thermo Fisher Scientific, A-21245], 1:500 Donkey anti-rat Alexa Fluor 594 [Thermo Fisher Scientific, A-21209]) in PBS for 3 h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... brain sections were washed in PBS-T 3×5min and incubated with the corresponding anti-rabbit or anti-mouse Alexa Fluor 568-conjugated and anti-rat Alexa Fluor 488-conjugated IgG secondary antibody (all 1:1000, Thermo Fisher Scientific, Waltham, MA, USA) for 2 hours at RT ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were seeded into custom built glass-bottom 35 mm dishes and imaged in Leibovitz’s L-15 medium (Invitrogen) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were eluted from the magnetic beads with 35 µl of 2x NuPAGE LDS Sample Loading Buffer (ThermoFisher, NP0007) with 100 mM DTT at 100 °C for 5 min ...
-
bioRxiv - Neuroscience 2021Quote: ... constructs in mammalian expression plasmids (0.5 – 3 µg/35-mm dish) were transiently transfected using Lipofectamine-2000 (ThermoFisher, #11668030) according to the manufacturer’s instructions with the exception that only half of the recommended amount of the reagent (5 µl ...
-
bioRxiv - Genomics 2021Quote: ... filtered through a 35 µm strainer and stained with 3x staining buffer (12 µM DAPI, 0.4 U/µl Ambion RNase Inhibitor ...
-
bioRxiv - Immunology 2021Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 50 resin (2.6 μm, 150Å; ThermoFisher Scientific). The scan sequence began with an MS1 spectrum (Orbitrap analysis ...
-
bioRxiv - Bioengineering 2021Quote: ... 10 μl from each sample was transferred to a 96-well flat bottom plate together with 100ul of Drabkin’s reagent (MilliporeSigma) containing Brij-35 solution (Thermofisher). The plate was incubated at room temperature for 15 min on a plate shaker ...
-
bioRxiv - Immunology 2021Quote: ... islets and tumors were maintained at 35-37°C with flow of 95% O2/5% CO2 saturated RPMI (Gibco). Islets and tumors were imaged on an Olympus FV100MPE microscope or a Leica SP8 DIVE upright multi-photon microscope as previously described (Friedman et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). The scan sequence began with an MS1 spectrum (Orbitrap analysis ...
-
bioRxiv - Plant Biology 2020Quote: The plasmid pMDC163 was modified by inserting a 35S CaMV promoter from pDONR207 by using a LR reaction (Invitrogen). The resulting plasmid ...
-
bioRxiv - Cancer Biology 2020Quote: ... This step was followed by incubation with 35 μl protein A/G agarose plus (50% slurry) (ThermoFisher Scientific 20421) for 6 hours ...
-
bioRxiv - Physiology 2022Quote: ... Transfer was set at 35 V for 1h30 in a Xcell II Blot module (catalog no. EI9051, ThermoFisher Scientific). After transfer ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were plated in 96-well or 35-mm-diameter culture dishes (Thermo Fisher Scientific, Inc., Waltham, MA, USA) for in vitro experiments after 24 h ...
-
bioRxiv - Plant Biology 2021Quote: Approximately 35% of each sample was analysed using an Orbitrap Fusion™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific) coupled to a U3000 nano-UPLC (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... coated glass-bottom dishes (Cellvis 35 mm - 14 mm micro-well #1.5 cover glass, Fisher Scientific, catalog no. NC0794151) for 120 min ...
-
bioRxiv - Cell Biology 2021Quote: ... the nerves were introduced into a 35-mm cell culture dish containing 2 ml of cold Leibovitz’s F-15 medium (Gibco) placed on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were imaged in 35-mm glass-bottomed dishes (constructed in-house) and imaged in Leibovitz’s L-15 media (Invitrogen) supplemented with 10% FBS ...
-
bioRxiv - Biophysics 2019Quote: ... BicD2N and dynactin were purified as previously described.35 Further information can also be found on Invitrogen's Bac-to-Bac Baculovirus Expression System Guide (Invitrogen). Briefly ...
-
bioRxiv - Genomics 2020Quote: ... The 100 μm capillary column was packed with 35 cm of Accucore 50 resin (2.6 μm, 150Å; ThermoFisher Scientific). The scan sequence began with an MS1 spectrum (Orbitrap analysis ...
-
bioRxiv - Molecular Biology 2019Quote: ... Purified DNA extracts were concentrated from approximately 35 μL to ~13 μL using vacuum centrifugation (SpeedVac, Thermo Fisher Scientific). Quantitation was performed on all donor reference samples purified with Promega’s Plexor ®
-
bioRxiv - Microbiology 2022Quote: ... Samples were loaded onto a trapping column (kept at 35°C) for desalting (Thermo Fisher Scientific, Dionex, Pepmap C18) and peptides separated on a 500 × 0.075 mm column (0.5 μl/min ...
-
bioRxiv - Developmental Biology 2022Quote: ... was added for 10-15 minutes on a rotary machine (PTR-35 360 vertical multi-function rotator, Thermofisher Scientific). Oocytes were then washed for 10 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... A 30 mL suspension culture of Freestyle 293F cells at 1×106 cells/mL were transfected with a 5:1:1 mass ratio of PB-T- CERTL(S132A):PB-RN:PBase (35 μg total DNA) using Freestyle MAX transfection reagent (Invitrogen) as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... neurons were transfected with 0.5–1.0µg plasmid DNA per coverslip in a 12-well plate (or 2–3µg DNA/35 mm dish) using Lipofectamine-2000 (Invitrogen) following manufacturer’s guide.
-
bioRxiv - Cell Biology 2024Quote: ... Lysates (35-40 µg) were separated by SDS-PAGE on NuPage 4-12% gels (Bis-Tris Gel; Invitrogen, #NP0335BOX), transferred to polyvinylidene fluoride membranes and blocked in 5% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). The scan sequence began with an MS1 spectrum (Orbitrap analysis ...
-
bioRxiv - Neuroscience 2022Quote: ... 35 μL/well of the neutralized samples were transferred in a final 384-well plate (Nunc catalog #264573; Sigma). The 384-well plate was heat-sealed and analyzed with the Erenna Immunoassay System.
-
bioRxiv - Biophysics 2023Quote: ... grids were placed in MatTek 35 mm/20 mm glass-bottom petri dishes and coated with 0.1 mg/mL poly-D-Lysine (Gibco) and incubated overnight in an incubator set at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... w/o phenol red) supplemented with 10% foetal bovine serum (FBS; 35-079-CV, Corning or 10270-106, Gibco), 5.5 mM glucose (25-037-CIR ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cell culture inserts were placed into 35 mm culture dishes containing 1.5 mL of 199 Earl’s salts (M199) medium (GIBCO, Invitrogen) supplemented with 5 mM HEPES buffer and 2% fetal bovine serum (FBS) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 15-35 μg of the lysate were run on SDS-PAGE gels and transferred using a iBlot2 apparatus (Invitrogen) on nitrocellulose membrane (Invitrogen ...
-
bioRxiv - Genetics 2023Quote: Passage 25-35 IMR90 primary lung fibroblast cell line acquired from ATCC (Cat#CL186) were grown in DMEM (ThermoFisher) supplemented with 10% fetal calf serum and 1% Pen/Strep ...
-
bioRxiv - Microbiology 2023Quote: ... after the last step of washing the beads were resuspended in 35 μl 1x LDS buffer (Thermo Fisher Scientific) containing 50 mM DTT and boiled for 5 min at 95 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Once the coverslips (Zeiss, 474030-9000-000) and 24×50 mm microscope slides (Thermo Fisher Scientific, 50-311-35) were pegylated they were assembled with double-sided tape as described above ...
-
bioRxiv - Biophysics 2023Quote: ... we pipette 3 ml collagen (0.6 - 2.4 mg/ml) solution with 200,000 cells into a 35 mm Nunc cell culture treated Petri dish (ThermoFisher Scientific). After incubation for one hour to ensure the complete polymerization of the gel ...
-
bioRxiv - Developmental Biology 2023Quote: Eyes were removed and collected in a 35 mm culture dish containing 3 mL of M199 medium (11825015, Gibco) supplemented with 2 mM L-glutamine (25030081 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were plated onto 13-mm coverslips in 35-mm dishes and transfected at 40% confluency with 1.6 μg of plasmid using Lipofectamine 2000 (Invitrogen).
-
bioRxiv - Molecular Biology 2023Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific) at a flow rate of 565 nL/min ...
-
bioRxiv - Synthetic Biology 2024Quote: Kinetic constants of SrIRED for cyclohexanone were determined in 100 mM Tris pH 8.0 with 0.5 mM NADPH and varying concentrations of cyclohexanone (0.06 mM - 35 mM) in transparent Nunc 96-well plates (ThermoFisher) and by monitoring the depletion of NADPH concentration at λ of 340nm (Spectramax 190 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Tgfβ-3(Cat# Mm00436960) alpha-SMA (Cat# Mm00725412) and Vegf (Cat# Mm00437306_m1) Single Tube TaqMan® Gene Expression Assays (Life Technologies Thermo Fisher Scientific) were used ...
-
bioRxiv - Bioengineering 2019Quote: ... from a single donor (22-year-old male) were expanded without further characterization in growth medium (GM) consisting of minimum essential alpha medium (α-MEM; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... On day 5, aggregates were transferred to MK10 medium (Minimum Essential Medium alpha [α-MEM, Thermo Fisher Scientific], 10% KSR (Thermo Fisher Scientific), 55 μM 2-mercaptoethanol ...
-
bioRxiv - Physiology 2019Quote: ... Bone ends were removed and the marrow was flushed by slowly injecting serum-free alpha minimal essential medium (αMEM; Invitrogen, Carlsbad, CA, USA) through a sterile 25-gauge needle into one end of the bone ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were cultured in a humidified atmosphere of 5% CO2 and 95% air at 37°C in an alpha modification of Eagle’s medium (Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2021Quote: RXRα activation by C26 was assessed using LanthaScreen™ time-resolved fluorescence resonance energy transfer (TR-FRET) RXR alpha coactivator assay (ThermoFisher Scientific #PV4797). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... were isolated from the periodontal ligament tissues (cells from 5 individuals were pooled to offset individual differences) and maintained in alpha minimal essential medium (α-MEM, GIBCO Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2022Quote: ... Primary human MSCs (Cyagen Biosciences, Suzhou, China) from bone marrow were cultured in a minimum essential medium alpha (MEM-α, Gibco, Carlsbad, CA) medium with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
-
bioRxiv - Immunology 2019Quote: Mice received an intravenous injection of 1 μg anti-CD45.2 PE-eFluor 610 monoclonal antibody (eBioscience) in phosphate buffered saline (PBS; Life Technologies brand, Thermo Fisher Scientific, Waltham, MA, USA). Mice were sacrificed after two minutes.