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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... and co-incubated with DYDDDDK Tag (FLAG epitope) Monoclonal Antibody (FG4R) (1:5000 in PBS with 10% Thermo Blocker BSA; Thermo Fisher catalog #MA1-91878, RRID: AB_1957945) overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... The primary (E-Cadherin [24E10] Rabbit mAB) and secondary (Goat anti-Rabbit Alexa 488) antibody was purchased form Cell Signaling Technology and Invitrogen (Thermo Fisher Scientific) respectively ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Immunology 2022Quote: The binding capacity of the glycoengineered COVA1-18 (2C1) hIgG1 mAbs was tested by directly coating Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific) O/N at 4°C with 100 µL 1 µg/mL purified SARS-CoV-2 RBD-protein ...
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bioRxiv - Immunology 2022Quote: ... OEP light cages were applied to export individual cross-reactive mAb secreting B cells directly into Dynabeads mRNA DIRECT lysis buffer (Life Technologies, 61011) in 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... the unbound xfR5 mAb was washed off by dialysis using Float-A-Lyzer™ G2 Dialysis Devices (Thermo Fisher Scientific; NH, USA) in cold PBS supplemented with 10mM hydroxylamine to quench any non-reacted NHS groups present on the xfR5 mAb conjugated D+T NPs (xfR5-D+T NPs ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once before being stained with surface mAb (Table S1) and viability dye LIVE/DEAD Fixable Near-IR (Thermo-Fisher Scientific, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... T-cells were incubated overnight at 4 °C with either mAb reactive to mouse phospho-CD247 (CD3 zeta) (Tyr111, Tyr123) (clone EM-55; Thermo Fisher Scientific) or mAb reactive to mouse ZAP70 phosphorylated at position 319 (clone 1503310 ...
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bioRxiv - Immunology 2023Quote: ... and next intracellularly stained with PE-conjugated anti-human phospho-Akt (Ser473) (D9E, CST) and anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) manually conjugated with Alexa 488 or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Microbiology 2023Quote: Monoclonal antibodies (mAbs) targeting β-GlcNAc-WTA (clone 4497) in different Ig isotypes and IgG subclasses were expressed in EXPI293F cells (Thermo Fisher) as previously described (29 ...
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bioRxiv - Immunology 2023Quote: ... anti-SIVmac251 Nef mAb (clone 17, epitope peptide corresponding to SIVmac239 Nef 71-80 not including the residue G63: Thermo Scientific/Pierce) manually conjugated with Alexa 488 or Alexa 647 ...
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bioRxiv - Immunology 2023Quote: ... they were resuspended in 0.5% BSA/PBS for prevention of experimental procedure-related loss and stained with mouse anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated in the dark for 30-45 min on ice with goat anti-mouse fluorochrome conjugated isotype specific mAbs (Life Technologies Corporation, Invitrogen, USA), washed twice and re-suspended in 2% BSA PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was further evaluated for G6PD expression using primary antibody against G6PD (Rabbit mAb # A11234) and the counter labelling anti-rabbit HRP secondary antibody (Invitrogen Catalog # 31460). Chemiluminescence was capture in iBright (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitations (IPs) were also performed from the same lysates with the (Neuromab) antibody mAb-α-iFGF14 conjugated to protein G dynabeads (Life Technologies), using previously described methods (Bosch et al. ...
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bioRxiv - Immunology 2024Quote: ... the mAbs following expression were purified and subjected to SDS-PAGE analysis using a NuPAGE 4-12% Bis-Tris gel (Thermo Scientific Inc) under reducing (lane 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... the same protocol as above was used except cells were treated with the following antagonists which were directly added to the conditioned media: AZD4547 (FGF receptor, Fisher Scientific #NC0660421, 100 ng/ml) or DMH1 (BMP receptor ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for gene expression analysis of known estrogen receptor target genes using a QuantStudio™ 3 Real-Time PCR System (Applied Biosystems™, CAT: A28567). All primers were purchased from Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in Coll I) (Gibco, A1048301) for 2 hours to overnight at 37 °C ...
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SIRT1 ameliorates premature senescence-induced defenestration in hepatic sinusoidal endothelial cellbioRxiv - Cell Biology 2020Quote: ... Primary rat HSECs were cultured in plates with medium comprising 80% MCDB131 (Gibco, 10372019) and 20% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rat IgG Alexa Fluor 488 (A11006) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2022Quote: ... The rat brains were removed and placed in ice-cold dissection media: HBSS (Gibco) supplemented with 30mM glucose ...
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bioRxiv - Molecular Biology 2020Quote: ... Larval fillet preparations were fixed using formaldehyde and stained with rat anti-mCD8 (ThermoFisher) at 1:100 followed by Alexa Fluor 488 anti-rat secondary antibody (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... and subsequent incubation with donkey anti-rat Alexa Fluor 555-conjugated secondary antibody (ThermoFisher) at a 1:500 dilution for 30 min on ice ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... GR1 and MAC1 in combination with sheep anti-rat IgG Dynabeads (Thermo Fisher Scientific) prior to the specific antibody staining ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in secondary donkey anti-rat ALEXA 594 (Invitrogen, Burlington, ON, Canada) overnight at 4°C ...
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bioRxiv - Pathology 2019Quote: ... sections were then incubated with either rat anti-F4/80 (Invitrogen, cat. number MF48000) (1:1000 in blocking buffer TBS with 2% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... A rat-specific primary antibody for skeletal muscle myosin heavy chain (MA1-35718, ThermoFisher) was diluted 1:5 in TBS-NGS ...
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bioRxiv - Neuroscience 2021Quote: ... the newborn rat hippocampi (< 24 h) were chopped and digested by 0.25% trypsin (Gibco) for 8 min in 5% CO2 incubator at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... stained again in 100 μl buffer with the anti-rat AlexaFluor488 (ThermoFisher Scientific, #A11006) secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: 3D culture was performed using rat tail Collagen I (Corning®, Fisher Scientific, France). The collagen I solution was neutralized using 1N NaOH and diluted in PBS to a final concentration of 1mg/ml for the contractility assay ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen) in blocking solution for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... rat astrocytes were plated at 6-8K per well in pre-warmed DMEM (Invitrogen), supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2019Quote: Full-length rat GluA1 and GluA2 cDNAs were subcloned into pcDNA3 (plasmid vector, Invitrogen) to generate pc3-GluA1 and pc3-GluA2 ...
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bioRxiv - Immunology 2022Quote: ... and Lin+ cells were depleted with sheep anti-rat IgG immunomagnetic beads (Dynabeads, Invitrogen). Cells were then immunostained and Lin- Flk2+CD27+ BM progenitors from F1 congenic strains were FACS purified (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... a mixture of rat tail collagen type I (Cultrex) and Matrigel™ (Life Technologies) was mixed with CMs and gamma-irradiated human foreskin fibroblasts (HFFs ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were Alexa Fluor®-488 goat anti-rat (Thermo Fisher Scientific™); Alexa Fluor®-647 goat anti-rat (Thermo Fisher Scientific™) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 555 goat anti-Rat IgG antibody (A21434) was purchased from Life Technologies. Hematoxylin (G1004 ...
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bioRxiv - Microbiology 2021Quote: ... Cotton rat lung epithelial cells (CRLC, ATCC PTA-3930) were grown in MEM (Gibco) supplemented as above except with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rat IgG (H+L) cross-adsorbed (Alexa Fluor 488, Life Technologies, A1106) and goat anti-mouse IgG2b cross-adsorbed (Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2020Quote: ... rat hippocampi and cortices were dissected from embryonic brains in ice-cold HBSS (GIBCO) supplemented with 10mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibodies were detected using goat-anti-rat-AlexaFluor594 secondary antibodies (Thermo Scientific) diluted 1:2000 in blocking buffer additionally containing 1 μg/ml DAPI for visualization of nuclei.
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bioRxiv - Cell Biology 2022Quote: HPAECs grown on glass coverslips coated with rat tail collagen I (Gibco, Gaithersburg, MD) were fixed in 2% parafolmadehyde ...
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bioRxiv - Plant Biology 2019Quote: ... Anti-rabbit Alexa Fluor® 488 and anti-rat Alexa Fluor® 568 (Invitrogen) were used as secondary antibodies.
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bioRxiv - Cell Biology 2019Quote: ... followed by Alexa Fluor-647 rat anti-mouse CD41 F(ab′)2 (Thermo Fisher) to detect endogenous mouse platelets 68 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then probed with HRP-conjugated goat anti-rat (Thermo Fisher Scientific, 31470) or anti-mouse (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human Zo1 (rabbit) and anti-Gfap (rat) were from Invitrogen (Carlsbad, CA, USA). Anti-Cdh5 (goat ...