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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: NHK/38 cells and sections of normal human epidermis were stained with a monoclonal anti-hIL-38 antibody (H127C) (14-7385-82, Thermo Fisher Scientific AG, 1/2000), followed by an Alexa Fluor 594-labeled goat anti-mouse IgG2b (115-585-207 ...
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bioRxiv - Cell Biology 2019Quote: A mAb producing CHOK1 cell line (CHOK1-mAb) was seeded at a density of 2 × 105 cells/ml in SFM-II media (Gibco, 12052098) in a Kuhner orbital shaker (170rpm ...
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bioRxiv - Immunology 2020Quote: ... Reactivity of the CIS43 mAb for the CIS43 VLPs was determined by measuring optical density at 450□nm (OD450) using an AccuSkan platereader (Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and subsequently stained for 1 h at room temperature with anti-NP mAb 1C7 antibody labeled with an Alexa488-fluorescent marker using the Alexa Fluor™ 488 Antibody Labeling Kit (Invitrogen). Immunofluorescence-labelled cells were washed twice in PBS-EDTA after incubation with the antibody and then subjected to flow-cytometry analysis using a Gallios flow cytometer (Beckman Coulter) ...
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bioRxiv - Immunology 2022Quote: ... Fc and non-digested mAbs were removed from the flow-through by a 2 h incubation at RT with 200 µl of protein A resin per mg of initial mAb (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... for the HC and LC of each mAb was transfected into 5ml of suspension Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific) in 50ml tubes ...
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bioRxiv - Biochemistry 2020Quote: ... primary CD4+ T cells were isolated from cones from anonymous donors with approval from the National Health Service Blood and Transplant (NHSBT) and stimulated with CD3 and CD28 mAbs on Dynabeads (Thermo Fisher) in the presence of 100 U/ml IL-2 to produce T cell blasts ...
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bioRxiv - Immunology 2020Quote: ... followed by staining with mAbs MM1a (anti-CD3) and ILA105 (anti-CD8) and secondary antibodies and Fixable Yellow Dead Cell Stain (Thermofisher Scientific). These cells were sorted using a FACSAria III (BD) ...
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bioRxiv - Immunology 2020Quote: ... the 5D5Δg Fab was produced by site-directed mutagenesis of the mAb 5D5 VH region using Accuprime Pfx Supermix (Thermo Fisher Scientific). 5D5Δg Fab was expressed in HEK293F cells and purified by chromatography as described above.
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bioRxiv - Microbiology 2021Quote: ... LS-C40832, rabbit polyclonal anti-RORC/RORC2 L-C116877 both from LSBio (Seattle, WA) and mouse mAb anti-c-Myc (ThermoFisher, 9E10).
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... cells were blocked for one h with a 10% FBS solution and stained for GXM with mAb 18B7 (10µg/mL) as a primary Ab and “Alexa Fluor® 633 Goat Anti-Mouse IgG” (Life Technologies) as a secondary Ab ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies). Immunolabelled coverslips were counterstained with DAPI (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... for 20 min before incubation overnight at 4°C in PBS-BR containing any of the following mAbs or reagents: DAPI nucleic acid (Molecular Probes), phalloidin Alexa fluor 488 (Molecular Probes) ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-claudin-4 mAb (#32-9400), rabbit anti-tricellulin mAb (clone 54H19L38, #700191) and rabbit anti-ZO-2 pAb (#71-1400) were purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Microbiology 2024Quote: ... Polprim and RPA -specific mAbs (approximately 100 µg of each ligand) were separately bound to 5mg of M-280 Tosyl activated Dynabeads (Invitrogen # 14203) O/N at 37⁰C on a rotating wheel ...
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bioRxiv - Immunology 2023Quote: The 14.4.4 mAb producing hybridoma cell line (isotype: mouse IgG2a, kappa) was maintained at 37 °C and 5% CO2 in RPMI (Life technologies) supplemented with 5% FCS (Biowest) ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Microbiology 2023Quote: Splenocytes were stimulated with the peptide pools described in the memory phenotypic assay and supplemented with anti-CD28 and anti-CD49d mAbs (ThermoFisher Scientific) both at 1.0 μg/ml or a tissue culture medium with 1% DMSO as a negative control ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-F or G mAbs were prepared under serum-free conditions using HyClone SFM4MAb (Thermo Fisher Scientific Inc., Rockford, IL) and purified by protein G-Sepharose affinity chromatography.
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Immunology 2023Quote: ... T cells were distributed into the round bottom 96 well plates at 3000 cells per well and stimulated by CD3/CD28 mAb-coated beads (ThermoFisher Scientific) at a ratio of 10:1 ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Genomics 2023Quote: A mAb producing CHOK1 cell line (CHO K1 mAb) was seeded at a density of 2 × 105 cells/ml in 50ml SFM-II media (Gibco, 12052098) in 8 replicate shake flasks in a Kuhner orbital shaker at 170rpm at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Microbiology 2024Quote: ... plates were coated with 50 µl of capture anti-FLAMOD monoclonal antibody (Mab, 9H10-R2-2E8 mouse IgG1 - lot vt220802-7034) at 5µg/ml in D-PBS 1X (PBS, Gibco 14190-094) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Biochemistry 2022Quote: ... Affinities of αE2 and αDHED to ERs were measured using the LantaScreen TR-FRET competitive binding assay screening protocol by the SelectScreen Biochemical Nuclear Receptor Profiling Service (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Immunology 2021Quote: ... and incubated overnight at 4°C with rat anti-mouse CD8 (4SM15, Invitrogen) and rabbit anti-mouse Ly6G (E6Z1T ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-MHCII (I-A/I-E) (ThermoFisher Scientific/eBioscience 14-5321-82).rat anti-ICAM2 (BD Biosciences 553326) ...
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bioRxiv - Developmental Biology 2020Quote: ... or anti-rat IgG all raised in donkey or goat (Thermo Fisher Scientific). DNA was stained with DAPI at final concentration of 20 µg/ml (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-TBR2 (clone: Dan11mag; eBioscience, Fisher Scientific, cat. no. 50-245-556), 1:200 to 1:250 ...
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bioRxiv - Cell Biology 2022Quote: ... rat and mouse IgG were used as secondary antibodies (Molecular Probes, Eugene, OR).
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bioRxiv - Neuroscience 2021Quote: ... with mRNA hybridized to an Affymetrix Rat Genome 2.0st Gene Chip (Affymetrix Inc.). Microarray chips were then used for gene expression analysis.
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bioRxiv - Evolutionary Biology 2019Quote: ... Alexa 488 and goat anti-rat or anti-mouse Alexa 647 (Molecular Probes), at 1:500 dilutions ...
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bioRxiv - Molecular Biology 2019Quote: ... and then incubated with Alexa 488-conjugated anti-rat IgG antibody (Thermo Fisher). Microvessel density was quantified in 10 fields/section ...
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bioRxiv - Plant Biology 2019Quote: ... secondary antibodies (Alexa Fluor 488 Goat anti-Rat IgM, ThermoFisher Scientific, A-21212) 1/100 in BSA 2% in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... or Alexa Fluor 488-conjugated donkey anti-rat IgG (H+L) antibody (ThermoFisher) at a dilution of 1:1000 for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... solution of 0.1 mg/ml in DPBS (rat tail collagen I, ThermoFisher, USA), and incubated for 30 min at 37°C in a humidified incubator ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were used at the following dilutions: rat anti-mCD8 (Invitrogen #14008185) 1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... cortical neurons were dissociated from dissected rat brains in 0.25% trypsin-EDTA (GIBCO), plated on 12-mm glass coverslips coated with poly-D-lysine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from rat cortical neurons using the TRIzol reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Secondary antibodies were goat anti-rat IgG(H+L)-HRP (Thermo Fisher Scientific) at a dilution of 1:5000 ...