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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... were incubated overnight at 4°C followed by the secondary antibodies (anti-rabbit 488, A11034 Invitrogen, 1:400; anti-mouse 555, A31570, Invitrogen, 1:400; DAPI, Roth, 1:400). Finally ...
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bioRxiv - Genetics 2021Quote: Samples containing equal amounts of total protein were mixed with 1 × Reducing Agent (Invitrogen by ThermoFisher) and 4 × NuPAGE LDS (lithium dodecyl sulfate ...
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bioRxiv - Genetics 2021Quote: Samples containing equal amounts of total protein were mixed with 1 × Reducing Agent (Invitrogen by ThermoFisher) and 4 × NuPAGE LDS (lithium dodecyl sulfate ...
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bioRxiv - Biochemistry 2022Quote: ... The western blot protein samples were eluted in 1× lithium dodecyl sulfate loading dye (Thermo Scientific) by boiling at 95 °C for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Protein bands were revealed with 1 h staining with SimplyBlue Safe stain (Thermo Fisher Scientific, LC6065) and overnight de-staining in water ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin immunocomplexes were precipitated for 1 hour at 4°C with blocked Dynabeads Protein G (Invitrogen), subjected to multiple wash steps and the chromatin eluted in elution buffer (50 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-DNA conjugates were cross-linked by the addition of methanol-free 1% formaldehyde (Life Technologies) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and proteins of interest were probed using polyclonal rabbit anti-His6 (1:2000; Invitrogen, Waltham, MA) and monoclonal mouse anti-RNAP (1:3500 ...
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bioRxiv - Cell Biology 2022Quote: ERα proteins were immunoprecipitated from J774a.1 cell lysates according to the manufacturer’s protocols (11204D, Invitrogen). The ERα antibody was the same as that used for Western blotting and was purchased from Santa Cruz (1:200 dilution) ...
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bioRxiv - Microbiology 2020Quote: ... 0.3 μl of a 1:10 dilution of SYPRO™ orange protein gel stain (ThermoFisher Scientific) was added to the mix ...
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bioRxiv - Bioengineering 2020Quote: ... Bound patient antibodies were then detected using Protein L-HRP (Thermo Fisher Scientific 32420, [1:5000]). Background from the raw ELISA signal in serum-treated wells was removed by first subtracting the signal measured in NeutrAvidin-alone coated wells then subtracting the signal detected in antigen-coated wells incubated with 1% nonfat milk + 100 nM competitor.
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bioRxiv - Cell Biology 2022Quote: ... Buffers for protein extraction were supplemented with 1×EDTA-free Halt protease inhibitor cocktail (ThermoFisher Scientific). Protein amounts were quantified using a BCA protein assay kit (ThermoScientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit antibodies against the following proteins were used: α-catenin (Thermo Fisher Scientific 711200; 1:100), pMRLC (Merck Millipore AB3381 ...
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bioRxiv - Pathology 2021Quote: ... inducible nitric oxide synthase (NOS2) and monocyte chemoattractant protein-1 (CCL2) were purchased from Applied Biosystems. Gene expression levels were determined with the comparative Ct quantitation method using Hypoxanthine-guanine phosphoribosyltransferase (Hprt1 ...
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bioRxiv - Bioengineering 2021Quote: ... Protein expression was induced via 0.5 mM isopropyl β-D-1-thiogalactopyranoside (ThermoFisher, Waltham, MA, USA) for 18 hrs (18°C ...
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bioRxiv - Genomics 2021Quote: IHC staining of four mismatch repair (NMR) proteins: MLH1 (1: 100, PA5-32497, Thermo Fisher Scientific), MSH2 (1 ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Immunology 2022Quote: ... Forty to eighty micrograms of protein samples were denatured in 1 × Bolt LDS Sample Buffer (Invitrogen) supplemented with 1 × Bolt Sample Reducing Agent (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... The GFP protein was probed with an anti-GFP antibody at 1:1,000 dilution (G10362, Thermofisher) and visualized using a goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... the protein concentration was quantified at a 1:200 dilution using a Bradford assay (Invitrogen, 23200).
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bioRxiv - Neuroscience 2024Quote: ... Enhanced Green fluorescent protein (GFP) was expressed from the pGREEN LANTERN™-1 vector (Life Technologies). The mCherry plasmid was obtained from Addgene (#84329 ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was pre-cleared for 1 hour at 4°C with protein A Dynabeads (Invitrogen). Chromatin samples were incubated overnight at a low temperature with 10 µg ChIP-grade anti-RNA polymerase II (ab26721 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membrane using a 1X transfer buffer (Thermo Fisher Scientific #NP0006-1) with 10% methanol at 30V for 3 hours at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5ug/mL of BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunoprecipitations were performed using 1-2ug of antibody bound to protein A/G magnetic beads (Thermofisher), incubated overnight at 4℃ ...
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bioRxiv - Cell Biology 2022Quote: ... the protein expression was induced with 0.2 mM isopropyl b-D-1-thiogalactopyranoside (IPTG, Thermo Scientific) at 16 °C for 24 h ...
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bioRxiv - Immunology 2023Quote: ... Protein was transferred to polyvinylidene fluoride (PVDF) membranes with transfer buffer (1× NuPAGE Transfer Buffer (Invitrogen) with 20% methanol ...
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bioRxiv - Systems Biology 2023Quote: ... the RNA underwent protein digestion by adding 1 mg of Proteinase K (PK, Thermo Fisher Scientific) to 25 mg of purified RNA and incubating it at 37 ℃ for 45 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Equal loading was confirmed by staining for >1 h in PageBlue™ protein staining solution (ThermoFisher).
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected by incubating samples in Alexa Fluor 568 conjugated streptavidin (1:1000; ThermoFisher) diluted in blocking solution for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were probed with a 1:2000 dilution of rabbit anti-GFP primary antibody (Invitrogen) in 10 ml TBST incubated 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to a pre-activated PVDF membrane in 1-Step Transfer buffer (ThermoFisher, 84731) using a semi-dry Pierce G2 Fast Blotter (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then 1 h at 4°C at 7-10 rpm with dynabeads protein A (Invitrogen), equilibrated with the EB ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein presence was visualized by adding PierceTM 1-StepTM Ultra TMB blotting solution (Thermo Fisher Scientific) to the membrane ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mg of cortex lysate supernatant was incubated with 30 μl of Protein G Dynabeads (Invitrogen) preincubated with the appropriate antibodies (the information for antibodies is in Table S2) ...
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bioRxiv - Cell Biology 2024Quote: ... and proteins were digested with 1 µg of Pierce MS-grade trypsin (90057; Thermo Fisher Scientific) and incubated overnight at 37 °C with agitation ...
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bioRxiv - Bioengineering 2024Quote: ... were resuspended in 1 mL of M-PER Mammalian Protein Extraction Reagent (78501, Thermo Fisher Scientific) for lysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were sonicated with a probe sonicator (10 × 0.5s pulses; Level 1) (Fisher Scientific, Waltham, MA), reduced in 10mM DTT for 30 minutes at room temperature (RT) ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IgM-HRP and anti-mouse IgA-HRP (ThermoFisher), goat anti-mouse Igκ ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Equivalent amounts of protein were resolved on precast polyacrylamide denaturing gels and were then transferred onto 0.2 μm PVDF membranes using either dry transfer with an iBLOT2 system (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a total of 100 µg of protein per sample was subjected for trypsin digestion ...
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bioRxiv - Cancer Biology 2020Quote: ... and protein was quantified using the Bradford protein assay (Thermo Scientific). For blotting ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was determined using the Qubit Protein Assay Kit (ThermoFisher). Oligonucleotide probes (Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein content was assessed using BCA Protein Assay Kit (Thermo Fisher). Equivalents of 20 μg of protein were taken for mass spectrometry (MS ...
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bioRxiv - Genetics 2021Quote: ... Protein concentrations were estimated with bicinchoninic acid protein assay (ThermoFisher Scientific). Ten mgs of each lysate were combined ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentrations were measured with Pierce 660nm Protein Assay (Thermo Scientific), and 10 μg of protein were loaded on 4–20% FastGene SDS-PAGE gradient gels (Nippon Genetics) ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A or protein G Dynabeads (Thermo Fisher Scientific, 10001D, 10003D) was used for rabbit or mouse antibodies respectively ...