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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with TBS-T and incubated for 1 h at room temperature with an HRP-conjugated anti-mouse (dilution 1:50,000, Invitrogen, G-21040) or anti-rabbit (dilution 1:50,000 ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Microbiology 2024Quote: ... a rabbit anti-GFP antibody (1:2000, Yeasen Biotechnology Co., Ltd) and a Mouse anti-β-actin antibody (1:2000, Invitrogen) were added and incubated at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Mouse anti-arm antibody at 1:1500 dilution or rabbit anti-FLAG antibody at 1:1000 dilution and goat anti-rabbit IgG–AP conjugate at 1:2000 or goat anti-mouse IgG-AP conjugate at 1:200 dilution (Molecular Probes) was used as primary and secondary antibodies to probe the blot ...
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bioRxiv - Cell Biology 2023Quote: ... horseradish peroxidase (HRP)-conjugated anti-mouse IgG (NA931, 1:10000), and anti-rabbit IgG (NA934, 1:10000) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in the appropriate secondary antibodies (1:2000) for 1 hour: cross-adsorbed goat anti-mouse IgG Alexa Fluor 488 (A28175) or 568 (Invitrogen, A11004), and cross-adsorbed goat anti-rabbit IgG Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated in 1% goat serum/PBST with Alexa Fluor 633 goat anti-mouse IgG antibody (Invitrogen, 1:200) at 4°C overnight.
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bioRxiv - Cell Biology 2023Quote: ... at room temperature for 1 hour and then incubated with mouse anti-V5 (1:5,000; Invitrogen, Cat# R960-25, RRID: AB_2556564) and mouse anti-tubulin (1:4,000 ...
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bioRxiv - Neuroscience 2023Quote: ... 4) with secondary Abs anti-mouse or rabbit IgG conjugated Alexa 488 (1:400) or Alexa 568 (1:200) (Life Technologies).
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated in anti-mouse or rabbit IgG conjugated Alexa 488 (1:400) or Alexa 568 (1:200) (Life Technologies) for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... cleared lysate (1 mL) was mixed with 1 µg of relevant antibody (Mouse IgA isotype control (eBioscience Invitrogen 14-4762-81) and Mouse IgA anti-AGO2 (Santa-Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... the tissues sections were washed with PBS and then incubated for 1 h at room temperature with the following secondary antibodies: anti-mouse Alexa Fluor 488 (1:600, ThermoFisher Scientific), anti-goat Alexa Fluor 555 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed in PBS pH 7.2 and incubated for 1 hour at 35°C with secondary antibodies diluted 1:250 in PBS buffer with 1% BSA (Alexa Fluor 488-labeled mouse anti-rabbit IgG (Molecular Probes)).
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bioRxiv - Cell Biology 2023Quote: ... cells were washed in 1 x PBS then incubated with Alexa Fluor 488 goat anti-mouse (1:500, Thermo Fisher Scientific), Hoechst 33342 (1:5000 ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies included horseradish-peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:2000 or 1:5000; ThermoFisher, 31430 or 31460). Proteins were detected by ECL chemiluminescence detection kits (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... the sections were washed three times with PBS 1X and then incubated for 1 hour with donkey anti-mouse (or anti-rabbit) Alexa Fluor 488nm (or 555nm) secondary antibodies (1:200, Thermofisher Scientific). DAPI (Roche ...
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bioRxiv - Microbiology 2022Quote: A 1 × 1 cm of mouse skin encompassing the wound site was excised into 2 mL of RNAlater stabilization solution (Invitrogen, USA) and incubated overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were washed as described above four times and then incubated on ice with 50 µl of secondary antibody solution (1% BSA in PBS with either HRP-conjugated anti-mouse 1:5000 (Invitrogen 31430) or anti-rabbit 1:5000 (Invitrogen 31460) ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa 594 donkey anti-mouse antibody (715-585-150; 1:400; Jackson Immuno Research Labratories) or Alexa 647 donkey anti-mouse (A31571; 1:400; Life technologies). For all secondary antibodies used ...
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bioRxiv - Bioengineering 2022Quote: ... sections were incubated for 1 h at RT with goat anti-mouse Alexa 555-conjugated antibody (1:300; A21434, Life Technologies). After counterstaining with hematoxylin slides were mounted under coverslips in Prolong Gold anti-fade reagent (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... antibodies were used followed by specific secondary Alexa Fluor antibodies (1:500 anti-mouse Alexa Fluor 546 and 1:500 anti-rabbit Alexa 488 Thermo Fisher). For actin staining ...
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bioRxiv - Physiology 2024Quote: ... Alexa Fluor 594 donkey anti-rabbit (1:500) and Alexa Fluor 488 donkey anti-mouse (1: 500; Molecular Probes, Cat# A-21207. RRID: AB_141637, and Molecular Probes, Cat# A-21202 ...
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bioRxiv - Genomics 2024Quote: ... the slides were washed 18×2 minutes in TBST and then incubated with primary antibodies at the dilutions indicated after the DAB reaction: IBA1 (1:1000, Rabbit, Wako, 019-19741) and PHF-TAU (1:1000, AT8, Mouse, Thermofisher, MN1020), washed as above and stained with species-appropriate secondary antibodies conjugated to an Alkaline Phosphatase (AP ...
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bioRxiv - Genetics 2024Quote: ... anti-mouse Alexa Fluor 488 (1:100, A11017, life technologies) and anti- rabbit Alexa Fluor 568 (1:100, A21144, Life technologies) were used ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated with the secondary fluorochrome-conjugated antibodies (donkey anti-mouse Alexa 488 1:500 [Thermo-Fisher Scientific, A-21202]; goat anti-rat Alexa 594 1:400 [Invitrogen]) for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 h followed by incubation with 1:2,000 diluted 6x-His tag monoclonal antibody (produced in mouse; Invitrogen; #MA1-21315) at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... the cells are washed three times with 1× PBS and incubated with secondary antibody anti-mouse AlexaFluor 546 (1:400 Thermo Fisher) for 1 h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... the slides were stained with the following secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG AlexaFluor 488-conjugated (1:200; Invitrogen, A11001) and donkey anti-rat IgG AlexaFluor 555-conjugated (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Spike protein (C9-tag) on the surface of pseudovirus was detected using a mouse anti-C9-tag monoclonal antibody 1D4 (Invitrogen, Cat. No. MA1-722), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... The presence of relevant proteins was visualized using Alexa Fluor secondary antibodies (donkey anti-mouse AF488, anti-rabbit AF555, A-31572, Thermofisher and anti-goat AF647).
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The concentrations of total protein and albumin in mouse BAL fluid were quantified following the manufacturer’s instruction via the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA) and Albumin (BCG ...
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bioRxiv - Cell Biology 2024Quote: ... slides were incubated with the following secondary antibodies: Alexa 488 goat anti-rabbit/mouse/Chicken (1:1000, Invitrogen A-11008 rabbit, A-11032 mouse, A-11039), Alexa 594 goat anti-mouse/rat (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... Cas9 protein (Invitrogen), a single-stranded 100-bp oligo donor nucleotide (ssODN ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein gel (Invitrogen). Antibodies were added according to the manufacturers recommended conditions ...
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... containing primary antibodies (rabbit against tyrosine hydroxylase, 1:1000, AB152, Merck Millipore; and chicken against green-fluorescent protein, 1:750, A10262, Invitrogen, Thermo Fisher Scientific) to allow for sufficient target protein-primary antibody interaction ...
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bioRxiv - Plant Biology 2022Quote: ... The protein extracts were boiled in sample buffer (1× NuPAGE™ LDS sample buffer, 1× NuPAGE® Reducing Agent [Thermo Fisher Scientific]) for 10 min at 90°C and loaded on a 4–20% (w/v ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 or A549-ACE2-TMPRSS2 cells were grown on coverslips seeded in 24-well plates and co-transfected with DsRed vector (1 μg/well) and S protein expression plasmid (1 μg/well) using Lipofectamine 2000 LTX with Plus reagent (Thermo Fisher Scientific) and OptiMEM medium (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... S-2P and RBD were biotinylated protein at a theoretical 1:1 ration using the Easylink NHS-biotin kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 1 mg of protein were incubated with anti-HA antibody (1:100) for 1 h at 4 °C followed by incubation with Protein G Dynabeads (Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... IgG-containing supernatants were diluted 1:1 with 1x phosphate-buffered saline [pH 7.4] and batch-bound to Pierce™ Protein A agarose (Thermo Fisher Scientific) overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Genetics 2024Quote: ... were incubated overnight at 4°C followed by the secondary antibodies (anti-rabbit 488, A11034 Invitrogen, 1:400; anti-mouse 555, A31570, Invitrogen, 1:400; DAPI, Roth, 1:400). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... The antibody-protein complexes were isolated by incubation with magnetic beads (Invitrogen Dynabeads protein A and protein G) for 6 hours at 4℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant containing nuclear proteins was measured for protein concentration using Pierce BCA Protein Assay Kit (Thermo Scientific) and stored at -80°C until use ...
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bioRxiv - Bioengineering 2023Quote: Protein yield: MSC-EV preparation total protein concentration was determined using the Micro BCA Protein Assay Kit (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... supernatant protein concentration was determined by BCA protein assay (Pierce BCA Protein Assay Kit, Cat#23225, Thermo Scientific). Twenty μg/lane was run (200V ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration was determined using the Qubit™ Protein and Protein Broad Range (BR) Assay Kit (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: Protein concentration in the protein sample was determined by using a BCA protein assay kit (Pierce, Thermo Scientific). Samples in aliquots of 100 μL each were stored at -80°C.