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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were quantified using BCA protein assay kit (Thermo Scientific). Lithium dodecyl sulfate loading buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... the protein concentrations were quantified by BCA protein assay (ThermoFisher, 23225). 20 μg protein were run on a 4-20% reducing SDS-PAGE Mini-PROTEAN TGX gel (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... with PBS and secondary antibodies (goat anti-rabbit AF 555, goat anti-rabbit AF 647, goat anti-mouse AF 555, goat anti-mouse AF 647; Invitrogen; 1:500 dilution in 0.5% Triton, PBS) were applied for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were incubated with secondary antibodies (FITC anti-mouse, 1:1000, Jackson Immuno Research, #715-545-150; AlexaFluor564 anti-rabbit, 1:500, Thermo Fisher, #A11035) for 2hr at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-tenascin C antibody (MT1, DSHB), anti-rabbit IgG Alexa 488 (ab150077, 1:500) and anti-mouse IgG Alexa 594 (A21203, 1:500; Invitrogen, CA, USA) were used for the immunofluorescence procedure ...
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bioRxiv - Neuroscience 2022Quote: ... anti-Brp/nc82 (mouse; 1:50, Developmental Studies Hybridoma Bank – see below) and/or anti-GFP (rabbit: 1:10,000; Life Technologies A-11122). The secondary antibodies used in all experiments except Figure S1 and Figure S6 were anti-mouse-Cy5 (1:250 ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibody incubation was for 1 hour at room temperature using HRP conjugated antibodies: anti-mouse IgG at 1:3000(Invitrogen 62–6520) or goat anti-rabbit IgG at 1:3000 (Promega W4011) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed immediately or stimulated at a ratio of 1:1 with Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1152D) for 16 hours (qPCR and RNAseq) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 2 hours prior to stimulation with ratio of 1:1 with Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1152D) for 48 hours ...
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bioRxiv - Immunology 2021Quote: ... and 1:1 as shown in Table 2 and stimulated at a ratio of 1:1 with Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1152D). After 5 days ...
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bioRxiv - Neuroscience 2020Quote: ... slices were rinsed 4 x 10 min in PBS at room temperature and then incubated in secondary antibodies (goat anti mouse Alexa Fluor 568, 1:1000 and goat anti rabbit Alexa Fluor 488, 1:1000; Molecular Probes, Thermo Fisher) for 120 min in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS and incubated in secondary antibody (anti-rabbit Alexa Fluor 546, 1:500, Invitrogen A-11010; anti-mouse Alexa Fluor 488 1:500, Invitrogen A-11001) for 1 hour at room temperature.
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bioRxiv - Neuroscience 2021Quote: ... Cells were then washed in PBS and incubated for 1 hour at room temperature with the appropriate fluorescent conjugated secondary antibodies: anti-mouse Alexa Fluor 488 (1:200, Thermo Fisher Scientific), anti-rabbit Alexa Fluor 594 (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... Nanobody expression was visualized by Western analysis using an anti-6xHis antibody (1:1000) (NeuroMab #75-169) and goat anti-mouse secondary (1:3000) (Invitrogen #62-6520).
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bioRxiv - Neuroscience 2021Quote: ... Membranes were subsequently incubated in blocking buffer containing HRP-conjugated anti-mouse IgG (H+L) (1:2000, Fisher 62-6520) or HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) for one hour ...
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bioRxiv - Microbiology 2020Quote: ... for 1 hour at room temperature followed by 1 hour staining with the secondary antibody anti-mouse-Alexa Fluor 488 (1:2000, Thermo Fisher Scientific), Hoechst (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were then incubated with secondary antibody (Alexa 647 goat anti-mouse, 1:100, Cat. A21235, Thermo Fisher; Alexa 647 goat anti-rabbit, 1:100, Cat. A27040, Thermo Fisher; Alexa Fluor 594 goat anti-rabbit ...
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bioRxiv - Microbiology 2021Quote: ... followed by 30 minutes with 0.1% Tween/PBS at 37°C and 1 hour at 37°C with secondary antibody Alexa-488 goat anti-mouse (1:500) (Life Technologies #A11029) in 1% BSA/PBS before mounting with Prolong Gold with DAPI (Invitrogen #P36935) ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... followed by incubation with DAPI (1:1000) and conjugated secondary antibodies anti-mouse Alexa555 and anti-rabbit Alexa647 (1:500, Thermo Fisher Scientific). Coverslips were mounted and samples were analyzed using a Leica SP8 Laser Scanning Confocal Microscope with a 63x oil objective (Leica ...
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bioRxiv - Immunology 2022Quote: ... washed 3x with PBS and treated with 50mM NH4Cl (15min, RT) prior incubation with anti-T6BP (Rabbit, 1:200, HPA024432) and anti-CANX (Mouse, 1:500, MA3-027, Thermo fisher scientific) in PBS containing 1% ...
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bioRxiv - Pathology 2021Quote: ... blots were incubated with an anti-influenza A NP protein antibody 19C10 generated by our laboratory (1:1000) and anti-V5 tag antibody (Thermo,1:5000) and visualized with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Invitrogen, 1:5000).
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bioRxiv - Biochemistry 2021Quote: ... Blots were incubated in primary antibodies (sheep anti-BPNT2, 1:1000, Invitrogen #PA5-47893; mouse anti-Actin, 1:1000, Invitrogen #MA1-744) overnight at 4C ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-PV, 1:2000, ref. 235, Swant, Switzerland; secondary antibody: Alexa 488 goat anti-mouse, 1:500, ref. A11029, Life Technologies; USA) and using the virus reporter mCherry co-labelling.
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed and incubated with a fluorescently-labeled secondary antibodies (1:200 goat anti-rabbit Alexa Fluor 488, Jackson Immunoresearch or 1:1000 goat anti-mouse IgG1 Alexa Fluor 555, ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... The following were used at 1:100 for IHC and 1:400 for ICC: Alexa fluor 555 donkey-anti-mouse (Invitrogen, A-31570), Alexa fluor 647 goat-anti-rat (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The tissues were incubated with Alexa dye-conjugated secondary antibodies (Alexa Fluor™ 488 goat anti-mouse IgG, 1:500, B40941, Life Technologies; Alexa Fluor™ 555 goat anti-rabbit IgG, 1:500, A27039, Invitrogen) and DAPI (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed 3x 5 minutes in PBST then incubated with anti-rabbit and anti-mouse antibodies (LI-COR, 1:15,000 or ThermoFisher, 1:10,000) diluted in 4% milk/PBST for 1.5 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... hASIC1a was detected using RHO 1D4 antibody (1 μg/μL, University of British Columbia) and 1:5000 goat anti-mouse IgG HRP-conjugate (Thermo Fisher Scientific). Biotinyl-PcTx1 was detected using 1:1000 rabbit anti-biotin antibody (abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated in primary antibodies against VPRBP (1:80) and fibrillarin (1:100) for 2h at RT followed by Alexa flour 594 Goat anti mouse (Invitrogen, Cat # A11020) or Alexa flour 488 Goat Anti R (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked for 30 min at room temperature in PBS containing 1% BSA and then incubated with mouse monoclonal anti-V5 antibodies (1:200 dilution, Invitrogen #R960-25) at 25°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 1:100).Cells were then washed three times in PBS and incubated in secondary antibodies (goat anti-rabbit Alexa Fluor 488; Invitrogen, 1:500, or goat anti-rabbit Alexa Fluor 488 and goat anti-mouse Alexa Fluor 594; Invitrogen, 1:1000) and DAPI (1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed with PBS-Tx and incubated with secondary antibodies (Alexa Fluor 488 donkey anti-mouse, 1:500, Life Technologies; Alexa Fluor 555 donkey anti-rabbit, 1:500, Fisher Scientific) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed with PBS three times for 10 minutes in PBS and incubated with secondary antibodies (respectively: 1:500 goat anti-rabbit IgG, Life Technologies, or 1:500 goat anti-mouse IgG1, Life Technologies) for three hours ...
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bioRxiv - Molecular Biology 2022Quote: ... the slides wash washed three times with 1×PBS and incubated for 30 min with secondary antibodies: Alexa Fluor 568 goat anti-mouse IgG (1: 100, Invitrogen/Molecular probes) and Alexa Fluor 488 goat anti-rat IgG (1 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed in stain buffer and secondary antibody staining (Alexa Fluor 488 donkey anti-rabbit IgG, A21206, Invitrogen, 1:100 and Alexa Fluor 568 donkey anti-mouse IgG A10037, Invitrogen, 1:100) was performed in 50 µL of stain buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed and incubated in the secondary antibody cocktail (1:200, anti-mouse; Alexa-594, Cat. # A11005 and 1:200, Alexa-488 anti-rabbit, Cat. # A11008; ThermoFisher Scientific, USA) for 4- 5hrs at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with the appropriate secondary antibodies (anti-rabbit-A488 Life Technologies, 1:1000; anti-mouse-A568 Life Technologies, 1:1000) for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed five times in 0.1% Triton and incubated for 1 hour with fluorescent secondary antibody (Alexa Fluor 568 anti-Mouse (Invitrogen, #A11005, 1:500) diluted in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... Wells were washed three times with PBS-T and incubated for 1 h with a 1/2000 dilution of a goat anti-mouse Alexa 555 secondary antibody (Invitrogen, Waltham, USA) in blocking solution ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse, Thermo Fisher Scientific #31430). After multiple washing steps ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were washed with PBS and incubated with secondary antibody (goat anti-mouse Alexa Fluor 555, cat#A21422Invitrogen, 1:250; goat anti-rabbit Alexa Fluor 647, cat#21244, Invitrogen, 1:250) for overnight at 4°C under agitation ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used (all at 1:100 dilution in immunofluorescence, 1:1000 in immunoblots): CX36 mouse anti-human (Invitrogen, clone 1E5H5), rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662) ...
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bioRxiv - Molecular Biology 2024Quote: ... each membrane was probed for using the following primary antibodies: mouse anti-CFTR 596 (1:1000) (UNC) or CAR anti-rabbit (CXADR) (1:100) (PA5-31175, Invitrogen, Waltham, MA) was applied for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... at 4°C for 16 h and fluorescent-labeled secondary antibodies (Alexa Fluor 488 goat anti-rabbit IgG, 1:1000 or Alexa Fluor 555 goat anti-mouse IgG, 1:1000, Thermo Fisher Scientific) for 30 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... blots were washed thrice with 1× TBS-T for 10 minutes each and incubated with HRP conjugated secondary antibody (anti-Rabbit HRP Invitrogen, #32460; 1:10,000 or anti-Mouse HRP Invitrogen, #32430; 1:10,000) prepared in 1×TBS-T for 1 hour at RT on the rocker ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed with 1x PBS before 2 h incubation of corresponding secondary antibody solutions containing either donkey-α-rabbit DyLight 488 (1:700, #SA5-10038, Thermo Fischer) and/or donkey-α-mouse DyLight 650 (1:1000, #SA5-10169, Thermo Fisher) in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were incubated with primary antibodies (Rabbit-anti-GFP: 1:1000, Thermo Fisher Scientific, Cat. #G10362; mouse-anti-c-myc: 1:200, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... sections were washed three times with PBS and incubated with Alexa 488 (labeled anti-mouse or rat, 1:400) or Alexa 594 (labeled anti-rabbit, 1:400)-conjugated secondary antibody (Invitrogen, Waltham, MA) for 1 h at room temperature ...