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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... slices were washed with PBS three times for 10 minutes in PBS and incubated with secondary antibodies (respectively: 1:500 goat anti-rabbit IgG, Life Technologies, or 1:500 goat anti-mouse IgG1, Life Technologies) for three hours ...
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bioRxiv - Molecular Biology 2022Quote: ... the slides wash washed three times with 1×PBS and incubated for 30 min with secondary antibodies: Alexa Fluor 568 goat anti-mouse IgG (1: 100, Invitrogen/Molecular probes) and Alexa Fluor 488 goat anti-rat IgG (1 ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed five times in 0.1% Triton and incubated for 1 hour with fluorescent secondary antibody (Alexa Fluor 568 anti-Mouse (Invitrogen, #A11005, 1:500) diluted in the same buffer ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with the appropriate secondary antibodies (anti-rabbit-A488 Life Technologies, 1:1000; anti-mouse-A568 Life Technologies, 1:1000) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed and incubated in the secondary antibody cocktail (1:200, anti-mouse; Alexa-594, Cat. # A11005 and 1:200, Alexa-488 anti-rabbit, Cat. # A11008; ThermoFisher Scientific, USA) for 4- 5hrs at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 1 h at room temperature in dark at the following dilutions: Donkey Alexa 555 α-mouse (1:1000, Invitrogen #A31570), Donkey Alexa 647 α-mouse (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... and incubated with the following antibodies for 1 hour at room temperature: goat anti-mouse 555 (A21422, 1:200, Thermo Fisher Scientific), or goat anti-rabbit 647 (A21244 ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Triton-X and 1:100 primary antibodies for cTnT (cardiac isoform Ab-1 mouse, Thermo Scientific™, cat. #MS-295-P0). After washing ...
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bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at room temperature for 1 hour with goat anti-mouse IgG conjugated to Alexa Fluor 568 (1:250, Invitrogen, cat # A11011). The antibody solution was removed ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed with 1x PBS before 2 h incubation of corresponding secondary antibody solutions containing either donkey-α-rabbit DyLight 488 (1:700, #SA5-10038, Thermo Fischer) and/or donkey-α-mouse DyLight 650 (1:1000, #SA5-10169, Thermo Fisher) in blocking solution ...
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bioRxiv - Genomics 2023Quote: ... blots were washed thrice with 1× TBS-T for 10 minutes each and incubated with HRP conjugated secondary antibody (anti-Rabbit HRP Invitrogen, #32460; 1:10,000 or anti-Mouse HRP Invitrogen, #32430; 1:10,000) prepared in 1×TBS-T for 1 hour at RT on the rocker ...
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bioRxiv - Immunology 2023Quote: ... The stained cells were then washed twice with 1ml cold FACS buffer (1% Bovine Serum Albumin [Sigma] and 1% Purified Mouse IgG [Invitrogen] in PBS) and fixed in 500μL of 4% paraformaldehyde at room temperature ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 Nucleocapsid antibody (produced in mouse) as a primary antibody was procured from (MA5-35943, Invitrogen, 1:1000-1:10,000), Rabbit phospho-p38 MAPK and total p38 MAPK Antibody were procured from (9211S and 9212S ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then incubated in 1 mL of primary antibody solution in blocking buffer (1:50 monoclonal mouse anti-nc82, Developmental Studies Hybridoma Bank, Iowa City, IA; or 1:500 rabbit anti-GFP, Thermofisher cat# A11122) at room temperature for 3 h in constant rotation ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated with secondary antibodies (goat anti-mouse alexa Fluor 568 1:500, Invitrogen A-11004 and goat anti-rabbit Alexa Fluor 488 1:400, Invitrogen A-11011) in PBS with 0.1% Triton X-100 for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the same procedure was followed but using anti-β-Tubulin (1:1000, DSHB, USA) as the primary antibody and Goat anti-Mouse AF 546 (1:300, Invitrogen, Germany) as secondary antibody.
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bioRxiv - Microbiology 2024Quote: ... incubated 1 h at room temperature with DAPI and conjugated secondary antibodies: anti-mouse IgG Alexa Fluor 488 (Life Technologies, 1:1000); anti-mouse IgG Alexa Fluor 555 (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... donkey anti-mouse Alexa Fluor 546 (1:500) or goat anti-rabbit Alexa Fluor 488 (1:500) secondary antibodies (Thermo Fisher Scientific). For staining with anti-centromere antibody ...
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bioRxiv - Systems Biology 2024Quote: Xenopus immunostaining was carried out as previously described102 with a primary antibody against beta-tubulin (1:100, DSHB E7) and a secondary goat anti-mouse fluorescent antibody (1:250, Life Technologies A32723).
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bioRxiv - Neuroscience 2024Quote: ... membranes were then incubated in appropriate secondary antibodies (goat anti-rabbit-HRP, Invitrogen G21234, 1:10,000; goat anti-mouse IgG1, Invitrogen PA174421, 1:5,000) for 30 minutes at room temperature before washing and enhanced chemiluminescence with (SuperSignal™ West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 μL fractions were pelleted and resuspended in 200 μL of chilled PBSA containing 1 μg mL−1 primary antibody (Anti-His tag mouse antibody, Invitrogen, Catalog # MA121315 or Anti-GUS rabbit antibody, Invitrogen, Catalog # A5790) and incubated for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... The plate was then washed four times with blocking buffer and incubated for 1 h at 37°C with HRP-conjugated goat anti-mouse IgG (H+L) secondary antibody (1/50 000, Invitrogen, cat# 31430). After washing six times with blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in secondary antibody (AlexaFluor 488 goat anti-mouse, 1:1000, Life technologies, #A21121; AlexaFluor 488 goat anti-chicken, 1:1000, Life technologies, #A32931 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed twice with PBST and incubated for 1 hour at room temperature in AF568 donkey anti-mouse and AF647 donkey anti-rabbit (both ThermoFisher, 1:1000) in 1% BSA in PBST ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated with the appropriate secondary antibody (Alexa Fluor 488–conjugated anti-mouse [1:1000; A-11001, Thermo Fisher Scientific], Alexa Fluor 488-conjugated anti-rabbit [1:1000; A-11034, Thermo Fisher Scientific] ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were washed with 1x PBS then incubated with secondary antibody for 1 hr at room-temperature: Alexa-488 conjugated a-mouse (1:500) and Alexa-594 conjugated a-rabbit (1:500) (Molecular Probes, USA). Slides were washed in 1x PBS and mounted in VectaShield mounting medium (Vector Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000) (Thermo Fisher Scientific). After rinsing three times with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then washed at least 4 times for 15 min in PBS and incubated overnight at 4◻°C on a slow shaker with Alexa-Fluor 488 goat anti-mouse IgG1 and α-bungarotoxin conjugated with Alexa-Fluor 594 (1:500 and 1:1,000 dilutions, respectively. Both from Invitrogen, Carlsbad, CA) to stain for acetylcholine receptor (AChR) ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed and incubated for 1 hour at room temperature with Alexa Fluor 488-conjugated anti-mouse antibody (1:1000; A28175, Thermo Fisher Scientific). Nuclei were stained with Hoechst 33342 (62249 ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were rinsed with PBS three times and incubated with a secondary antibody solution containing 1 µg mL-1 Goat anti-Mouse IgG Alexa Fluor 488 (Fisher Scientific, Spain) for another 20 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... This was followed by four washes with 200 μL of chilled PBSA and incubation with the secondary antibody in 200 μL of PBSA containing 1 μg mL−1 (AlexaFluor 467 goat anti-mouse, Invitrogen, Catalog # A32728 or AlexaFluor 467 goat anti-rabbit, Invitrogen, Catalog # A21245) for 1 h at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... FISH analyses were carried out by using digoxigenin-labelled DNA [human COT-1 DNA/mouse COT-1 DNA (Invitrogen, Carlsbad, CA, USA) and IGK-BAC (CH17-216K2)] and biotin-labelled DNA [human COT-1 DNA/mouse COT-1 DNA and IGH-BAC (CH17-212P11)] as described previously29 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by fluorescent secondary antibodies (1:1000 donkey anti-rabbit AlexaFluor 594, A21207, Molecular Probes; 1:1000 goat anti-mouse AlexaFluor 488, A11001, Molecular Probes); slides were coverslipped with Prolong Diamond mounting media (P36970 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by washes and incubation for 1 h at RT with a secondary antibody conjugated to Alexa 488 or 568 (anti-rabbit Alexa 568, A11011, Invitrogen, 1/2000 and anti-mouse Alexa 488, A11029, Invitrogen, 1/2000). 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were then orthotopically injected with drug-naïve MCF-7 cells (8×106 cells per mouse) suspended in 1:1 ratio of PBS and Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Scientific, A1413201) directly into the abdominal mammary fat pad ...
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bioRxiv - Microbiology 2023Quote: ... at 4°C and incubated with mouse anti-HA mAb clone 12CA5 conjugated and crosslinked to protein A/G magnetic agarose beads (Pierce; Rockford, IL. Thermo Fisher Scientific. Waltham, MA, USA). After rotation at 4°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: Membrane and cytosolic proteins were isolated from frozen mouse liver or small intestine (30mg) using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, Catalog no. 89842). Membrane and cytosolic fractions were separated by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Mm00440280_g1 (mouse, ThermoFisher), and TaqMan™ Universal Master Mix II ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse laminin (Gibco) was then added and incubated at 37°C for 4 hours ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... mouse (Affymetrix, 902931), performed by Cnkingbio Biotech Co. ...
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bioRxiv - Cell Biology 2021Quote: ... GST (Mouse, Invitrogen), GM130 (Mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse (Invitrogen, A315570), and rabbit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... mouse (ThermoFisher, #23017015); Vitronectin ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein quantification of the glass bead protein extracts was conducted using the BCA Protein Assay kit (Thermo Fisher), and samples with equal amount of proteins were applied on SDS-PAGE gels ...
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bioRxiv - Neuroscience 2020Quote: ... and pre-cleaned with protein A/G beads (Dynabeads™ Protein A, 10002D, Dynabeads™ Protein G, 10004D, Invitrogen). Immunoprecipitation was conducted with a mixture of Aβ antibodies (6E10 ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Microbiology 2021Quote: Purified ThsA protein was freshly prepared and protein concentration was quantified using Qubit Protein Assay Kit (ThermoFisher cat # Q33212). Protein was diluted in wash buffer to a concentration of 147 ng/μl ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...