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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... and 6.25 μg mL−1 were isolated by the hot acid-phenol method27 followed by treatment with Turbo DNA-free™ kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and the pellets were resuspended in 1 ml staining buffer supplemented with 0.5 μM Sytox Green Nucleic Acid Stain (Molecular Probes, Life Technologies), followed by incubation in the dark for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... Tryptic peptide mixtures were injected automatically and loaded at a flow rate of 20 μl/min in 0.1% formic acid in LC-grade water onto an analytical column (Acclaim PepMap C18, 75 μm x 25 cm; Thermo Scientific). The peptide mixture was separated by a linear gradient from 5% to 38% of buffer B (0.1% formic acid in ACN ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 μm ID x 15 cm, 2 μm, Thermo Scientific) mounted on a DPV ion source (New Objective ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 16 h and then the digest reaction was quenched with 1% formic acid (Fisher Scientific, Czech Republic). The resulting peptides were desalted using a C18 column (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... The phospho-enriched samples were again evaporated to dryness and then resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in 1% NP-40 lysis buffer (58) and protein levels were quantified using the Bicinchoninic acid protein assay (Thermo Scientific). Samples were run on SDS-PAGE gels before being transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was replaced with EB medium (DMEM with 15% FBS, 1% Non-Essential Amino Acids and 0.1 mM β-mercaptoethanol, Gibco 31350010). After 4–7 days ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting fractions were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MS/MS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain, Thermo Scientific). Each lane from the gel was cut and placed in 1.5 ml tubes ...
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bioRxiv - Microbiology 2020Quote: ... The phospho-enriched samples were evaporated to dryness and then resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) as above.
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bioRxiv - Microbiology 2020Quote: ... the peptides from each gel slice in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Cell Biology 2020Quote: ... were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... were evaporated to dryness and resuspended in 1% (v/v) formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... The phospho-enriched samples were again evaporated to dryness and then resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion (Noordstra et al. ...
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bioRxiv - Pathology 2020Quote: ... Samples were dried and re-suspended in 50 μL 1% formic acid and then subjected to liquid chromatography on Ultimate 3000 RSLC nano-system (Thermo Scientific) fitted with a 3 Acclaim PepMap 100 (C18 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting fractions were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequently a copy of chromosome XV was removed by counter-selection against the URA3 gene by replica-plating on SD medium containing 1 mg/mL 5-fluoroorotic acid (5-FOA, ThermoFisher Scientific) from a 100 mg/mL stock in DMSO ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were stained for DNA using either SYBR Green I Nucleic acid gel stain Ex504/Em523 (10,000 X concentrate in DMSO – subsequently diluted to 1:1000) (Invitrogen, Thermo Fisher, UK) or SYTO 9 Green Fluorescent Nucleic acid stain Ex485/Em498 ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Tryptic peptide mixtures were injected automatically and loaded at a flow rate of 20 μl/min in 0.1% formic acid in LC-grade water onto an analytical column (Acclaim PepMap C18, 75 μm x 25 cm; Thermo Scientific). The peptide mixture was separated by a linear gradient from 5% to 38% of buffer B ( 0.1% formic acid in ACN ...
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bioRxiv - Cell Biology 2021Quote: ... which were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific).
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bioRxiv - Systems Biology 2023Quote: ... Nuclei were resuspended in 400 µL of sort buffer (1% fatty acid-free BSA, 1x protease inhibitors (Thermo Fisher Scientific, PIA32965), 1U/µl RNAsin (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... PBS was replaced with a 1:6 (weight: volume) solution of 0.5 M acetic acid (984303; Thermo Fisher, Waltham, MA, USA) with 1 mg/ml pepsin (20343 ...
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bioRxiv - Cell Biology 2023Quote: ... containing 4.5 gl-1 glucose, GLUTA-PLUS and sodium pyruvate (Wisent, 319-027-CL) supplemented with 100 μM non-essential amino acids (Gibco, 11140050) and 10% fetal bovine serum (Wisent ...
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bioRxiv - Cancer Biology 2022Quote: ... Bicinchoninic acid (BCA) assay kit for protein quantification and MitoProbe JC-1 Assay kit for flow cytometry were procured from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then fixed with the formic acid solution for 1 hour at room temperature before the EdU detection protocol was performed as per manufacturers suggestions (Thermo Scientific). Following EdU detection ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by a 5 min incubation with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) and revealed using a Chemidoc MP Immaging System (BIO-RAD) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were dried and re-suspended in 50 μL 1% formic acid and then subjected to liquid chromatography on Ultimate 3000 RSLC nano-system (Thermo Scientific) fitted with a 3 Acclaim PepMap 100 (C18 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Immunology 2024Quote: ... 1 × TE buffer (5 mM Tris base, pH 8.0 and 1 mM EDTA acid) containing 15% w/v PEG-8000 (Fisher Scientific, BP2331) and 510 mM NaCl was added to the SQB sample at 1:1 volume and mixed gently via pipetting ...
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bioRxiv - Neuroscience 2022Quote: ... 1% penicillin-streptomycin and 1% non-essential amino acids) as described previously (37) and transduced using CytoTune™ –iPS 2.0 Sendai Reprogramming kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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bioRxiv - Synthetic Biology 2022Quote: The gel shift assay was run as a 1% agarose gel and post stained with SYBR Gold nucleic acid stain (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... 20 ng/ml Oncostatin M and 1% (v/v) MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, catalogue no. 11140050), 10 uM DAPT for another 10 days with the replacement of media every 48h ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Neuroscience 2023Quote: ... and then separated using a 180 min RP gradient (4–30% acetonitrile/0.1% formic acid) on an Acclaim PepMap100 RSLC C18 analytical column (2 μm, 75 μm id x 50 cm, [Thermo Scientific]) with a flow rate of 0.3 μl/min ...
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bioRxiv - Plant Biology 2023Quote: ... Dried samples were resuspended in 10% methanol and 1% acetic acid in water and analysed via ESI-based mass spectrometry using a Q-Exactive mass spectrometer (Thermo Scientific) as mentioned above.
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Cancer Biology 2023Quote: ... Fractions were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). All spectra were acquired using an Orbitrap Fusion Tribrid mass spectrometer controlled by Xcalibur software (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Dried samples were resuspended in 10% methanol and 1% acetic acid in water and analysed via ESI-based mass spectrometry using a Q-Exactive mass spectrometer (Thermo Scientific) by infusing through heated electrospray ionization (HESI ...
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bioRxiv - Bioengineering 2024Quote: ... we carefully aspirated the media from the microwells containing cancer spheroids and added 500 µl of RPMI media supplemented with a 1:5000 dilution of SYTOX™ Deep Red Nucleic Acid Stain dye (#S11381, Invitrogen) for tracking dead cells ...
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bioRxiv - Immunology 2022Quote: ... were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... The resulting fractions were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific).