Labshake search
Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... 2% MEM Non-Essential Amino Acids (Gibco 11140050), and 25mM HEPES buffer (Gibco 15630080) ...
-
bioRxiv - Developmental Biology 2024Quote: ... containing 0.1mM non-essential amino acids (NEAA) (Gibco), 2mM Glutamine (Gibco ...
-
bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... mixed with forward and reverse detection primers (T3DCD S1 forward [5’- TACGCGTTGATCACGACAAT-3’] and T3DCD S1 reverse [5’- TGGCGAGATTATTCCCTGAC-3’] or GAPDH forward [5’- ACCCAGAAGACTGTGGATGG-3’] and GAPDH reverse [5’- GGATGCAGGGATGATGTTCT-3’]) and SYBR Select Master Mix (Applied Biosystems), and then subjected to PCR using the StepOnePlus Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
-
bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
-
The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
-
bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
-
bioRxiv - Microbiology 2020Quote: ... The combined samples were dissolved in loading buffer [1% ACN and 1% formic acid (FA)] and analyzed using an EASY-nLC 1200 ultra-performance liquid chromatography system (Thermo Fisher Scientific, San Jose, CA, USA) equipped with a self-packed capillary column (75 μm i.d ...
-
bioRxiv - Developmental Biology 2023Quote: ... were cultured in mEC medium (0.1% bovine brain extract (BBE)/10% FBS/1%penicillin-streptomycin/1% Non-Essential Amino Acid (NEAA)/Dulbecco Modified Eagle Medium (DMEM) (DMEM Cat#: 41966-029, Gibco; BBE Cat#: CC-4098, Lonza BioScience). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO2 (HDLECs and HUVECs ...
-
bioRxiv - Cancer Biology 2021Quote: ... Culture medium for HT-1197 and HT-1376 was supplemented with 1x non-essential amino acids and 1 mM sodium pyruvate (Gibco). All culture media were supplemented with 10% fetal bovine serum (Corning ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were separated by electrophoresis on a 6% bis-Acrylamide (19:1) gel that was stained overnight with 1X SYBR green I nucleic acid gel stain (Invitrogen). The gel was imaged and quantified using an AlphaImagerHP (Alpha Innotech) ...
-
bioRxiv - Developmental Biology 2021Quote: ... A second RNA extraction was performed by adding 250uL of Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) (Ambion, Cat#AM9720) following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA was then extracted by mixing samples with Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) (Thermo Fisher, AM9722), incubating at 65°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
-
bioRxiv - Cancer Biology 2020Quote: ... U87 and SH-SY5Y cells were grown in DMEM additionally supplemented with 1% non-essential amino acids (Invitrogen, Karlsruhe, Germany).
-
bioRxiv - Microbiology 2021Quote: ... media for Calu-3 and Caco-2 cells were supplemented with 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (GIBCO). Cell lines were validated by STR-typing ...
-
bioRxiv - Physiology 2020Quote: ... EDL from both legs were dissected and maintained in 4°C Krebs–Henseleit buffer supplemented with 1×MEM amino acid mixture (Invitrogen) and 25mM glucose ...
-
bioRxiv - Neuroscience 2020Quote: The cDNA sequence corresponding to amino acids 1-192 of rab26 was amplified by PCR and cloned into the pET28a (Invitrogen) vector for protein expression ...
-
bioRxiv - Neuroscience 2021Quote: ... Perk+/+ and Perk-/- mouse embryonic fibroblasts (MEFs) were cultured in DMEM supplemented with 1% MEM non-essential amino acid (Gibco). Ire1α+/+ ...
-
bioRxiv - Bioengineering 2022Quote: ... resuspended in RPMI growth media (RPMI supplemented with 10% FBS, 1x non-essential amino acid solution [Gibco 11140050], 1 mM sodium pyruvate [Gibco 11360070] ...
-
bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 cells were cultured in the same medium with the addition of 1% Non-Essential Amino acids (Gibco). Cells were grown at 37°C with 5% CO2 in a humidified atmosphere ...
-
bioRxiv - Immunology 2020Quote: ... modified with 1% (v/v) each of minimum essential media (MEM) non-essential amino acids (Gibco; Grand Island, NY, USA), sodium pyruvate (Gibco ...
-
bioRxiv - Genomics 2021Quote: ... Colonies were washed with DMEM/F12 several times and allowed to settle by gravity then were plated in embryoid body formation media (DMEM/F12 supplemented with 2mM GlutaMAX, 1% non-essential amino acids (Thermofisher), 50nM 2-mercaptoethanol (Gibco) ...
-
bioRxiv - Cancer Biology 2020Quote: ... HPAF-II and EGI-1 cell lines were cultured in MEM media with Earle’s salts and non-essential amino acids (Gibco #10370021), AsPC-1 and COLO 320HSR cells in RPMI (Nacalai Tesque #0517625) ...
-
bioRxiv - Cancer Biology 2022Quote: ... peptides in 1% (v/v) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (ThermoFisher Scientific, Inc). After washing with 0.5% (v/v ...
-
bioRxiv - Microbiology 2022Quote: ... were incubated in serum or colistin diluted in RPMI buffer in the presence of 1 μM SYTOX Green Nucleic Acid stain (ThermoFisher). Bacteria were incubated at 37 °C under shaking conditions ...
-
bioRxiv - Molecular Biology 2023Quote: ... and a cDNA with an internal deletion such that the resultant cDNA encodes amino acids 1-178 and 1025-1099 were digested and subcloned into the NotI and XhoI sites of pcDNA4/TO (Invitrogen) modified to include N-terminal 3×FLAG-2× STREP tags to create plasmids 3×FLAG-2×STREP-Zfp106[Cterm] ...
-
bioRxiv - Neuroscience 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap® C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
-
bioRxiv - Biophysics 2022Quote: ... 1 mL of chloramphenicol-treated cells were stained for 15 minutes with 300 nM SYTOX Orange Nucleic Acid Stain (Invitrogen). The excess of SYTOX Orange was washed in tethering buffer before proceeding with FCS experiments ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... we then removed the mesh and marbles and transferred the remaining water and dust suspension to acid-washed 1 L bottles (Nalgene Nunc International Corporation ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding full-length human ADNP2 (amino acid residues 1–1131) was PCR amplified and cloned into a pFast-Bac-derived vector (Invitrogen) in frame with an N-terminal His6-tag ...
-
bioRxiv - Molecular Biology 2024Quote: ... Neuroblastoma SH-SY5Y cells were cultured in the same conditions and the same medium but supplemented with 1 mM of non-essential amino acids (Gibco). The total culture surface was 4500 cm² ...
-
bioRxiv - Biochemistry 2023Quote: ... The gels were stained in 1xTBE with 1:20,000 SYBRTM Gold nucleic acid stain (Thermo Fisher Scientific Corp, Waltham MA) for 30 minutes and imaged on a ChemiDoc MP system (Bio-Rad Laboratories Inc. ...
-
bioRxiv - Biochemistry 2023Quote: ... The gels were stained in 1xTBE with 1:20,000 SYBRTM Gold nucleic acid stain (Thermo Fisher Scientific Corp, Waltham MA) for 30 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
-
bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
-
bioRxiv - Microbiology 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Fisher Scientific). After washing with 0.5% (vol/vol ...
-
bioRxiv - Plant Biology 2022Quote: ... a 1/200 dilution of cells were transferred to fresh media containing 1X SYBR Green I Nucleic Acid Gel Stain (Invitrogen, now Theremo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
-
bioRxiv - Bioengineering 2023Quote: Cytotoxicity measurement was performed by incubating BL21 Star (DE3) or HEK293T cells with 1 μM SYTOX Blue nucleic acid stain (Invitrogen) for 5 min before imaging by a 405 nm laser irradiation.
-
bioRxiv - Microbiology 2023Quote: ... The cleared lysates were passed through a 0.45-μm filter and incubated with 1 ml of Ni-NTA (nitrilotriacetic acid) resin (Thermo Scientific) at 4°C over-night (O/N ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by incubating the gel with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) in 1X TBE for 5-10 min and reveal using a Chemidoc MP Immaging System (BIO-RAD) ...
-
bioRxiv - Systems Biology 2023Quote: ... The samples were resuspended in 500 μL of Acid-Phenol:Chloroform (with IAA, 125:24:1, pH 4.5, Thermo Fisher Scientific), 500 μL of Buffer B (200mM NaCl (DOT Scientific) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Purification was performed using 1 mL of Ni2+-nitriloacetic acid (NTA) resin (HisPurTM Ni-NTA resin, Thermo Fisher Scientific Co.) loaded onto 10 mL-Pierce™ disposable columns ...
-
bioRxiv - Physiology 2024Quote: ... hearts were loaded with 1 mg/ml Rhod-2 AM in 0.5 mL of DMSO containing 10% pluronic acid (Molecular Probes) followed by 50 µL 1 mg/ml RH237 in DMSO (Molecular Probes) ...
-
bioRxiv - Neuroscience 2024Quote: ... solutions prior to being placed in developer solution (2.5% sodium carbonate, 0.1% ammonium nitrate, 0.1% silver nitrate, 1% tungstosilicic acid, 0.7% formaldehyde (Thermo Fisher Scientific, MA, USA). Slides were treated with 0.5% acetic acid to stop the reaction ...
-
bioRxiv - Microbiology 2024Quote: ... 750 mM aminocaproic acid, 2 mg/ml lysozyme, 1 mM EDTA, 1.43 mM BME, 1x EDTA-free Pierce Protease Inhibitor [Thermo Scientific]) and incubated 20 min on ice ...
-
bioRxiv - Microbiology 2024Quote: ... bacteria were adjusted to an OD600 of 0.05 and mixed with 10 % normal human serum (NHS) (serum was prepared as described in41) in the presence of 1 µM SYTOX Green Nucleic Acid stain (ThermoFisher) in RPMI ...