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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: PBMCs were resuspended in PBS at roughly 107 cells/ml and labeled at room temperature with 5,6-carboxyflourescein diacetate succinimidyl ester (CFSE; Molecular Probes, Eugene, OR) at 1.25μM for 4 mins ...
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bioRxiv - Cell Biology 2021Quote: The size and shape of the observation volume was calibrated daily by measuring Alexa Fluor™ 488 NHS Ester (A20000, Molecular probes, Thermo Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... The two readout probes targeting a given amplifier set were pooled together and then coupled to one of four NHS-ester dyes (Atto 488, ATTO-TEC, AD 488-31; Cy3, Sigma-Aldrich, GEPA23001; Alexa Fluor 594, ThermoFisher, A20004 ...
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bioRxiv - Neuroscience 2020Quote: ... or hFc were labeled with Alexa Fluor 647 (AF647) using the Alexa Fluor 647 NHS Ester labeling kit (ThermoFisher Scientific, Cat #A20006) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: SpRecAA488 was made by covalently modifying primary amines (lysines or N-ter) of the protein with Alexa 488-succinidimyl ester (Molecular Probes, ThermoFisher), in presence of an excess of ssDNA (M13 mp18 ...
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bioRxiv - Microbiology 2022Quote: ... ME) using the EasySep™ mouse T cell isolation kit (StemCell, Cambridge, MA) and labeled with carboxyfluoroscein succinimidyl ester (CFSE; Life Technologies) as described previously [18] ...
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bioRxiv - Cell Biology 2021Quote: ... Up to 107 PBMCs were resuspended in PBS and incubated with either CellTrace Violet (CTV) or CellTrace carboxyfluorescein succinimidyl ester Kit (CFSE, both Thermo Fisher Scientific) in a final concentration of 5 μM.
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bioRxiv - Synthetic Biology 2020Quote: Cell growth and multiplication was assessed by Fluorescence Assorted Cell Sorting and fluorescence microscopy with 6-Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE, Life Technologies, USA) as a probe ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Ag83 parasite growth and multiplication were assessed by Fluorescence Assorted Cell Sorting and fluorescence microscopy with 6-Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE, Life Technologies, USA) as a probe ...
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bioRxiv - Microbiology 2021Quote: ... surface lysines on MS2 capsids were modified with fluorescent dyes (NHS-Atto488 or NHS-Atto633) and the heterobifunctional maleimide-PEG24-N-hydroxysuccinimide ester crosslinker (Thermo Fisher Scientific). Second ...
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bioRxiv - Immunology 2021Quote: ... were covalently coupled to Luminex microplex carboxylated bead regions (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher Scientific) according to manufacturer recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... was labeled with an anti-LNPK antibody (Wang et al., 2016) directly labeled with Alexa Fluor 568 NHS ester (Thermo Fisher #A20003). The ER was also probed with labeled anti-Protein disulfide-isomerase A3 (PDIA3 ...
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bioRxiv - Immunology 2020Quote: ... One population was pulsed with 50 μM of a relevant peptide and labeled with a high concentration (2.5 μM) of carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Eugene, OR). The other population was unpulsed and labeled with a lower concentration (0.25 μM ...
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bioRxiv - Immunology 2021Quote: ... were covalently coupled to Luminex microplex carboxylated bead regions (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher Scientific) according to manufacturer recommendations ...
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bioRxiv - Immunology 2022Quote: Allogeneic CD4 T cells were isolated from PBMC using a positive-selection T cell isolation kit and labeled with 5mM/ml carboxyfluorescein diacetate succinimidyl ester (CFSE) as per protocol (C34554, Molecular Probes, Life Technologies). Enriched pDC were exposed to LPS with or without HIVMN AT-2 stimulation in the presence of 10ng/ml Interleukin-3 (IL-3 ...
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bioRxiv - Molecular Biology 2022Quote: ... HtrA2-S306A at 185 µM was labeled with a 2-fold molar excess of BODIPY FL NHS ester (Thermo Fisher Scientific, D2184) for 60 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... Labeled tubulin was prepared by incubating polymerized microtubules with tetramethylrhodamine succinimidyl ester (C-1171; Thermo Fischer) or NHS-LC-biotin (21336; Thermo Fisher Scientific) for 30 min at 37°C (32) ...
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bioRxiv - Neuroscience 2022Quote: ... and their CA1 region bolus-stained with the Na+ sensitive dye SBFI-AM (sodium-binding benzofuran isophthalate-acetoxymethyl ester; Invitrogen, Karlsruhe, Germany). SBFI was alternatively excited at 340/380 nm at an imaging frequency of 0.5 Hz and emission was collected >440 nm from defined regions of interest (ROIs ...
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bioRxiv - Physiology 2022Quote: ... 2,7-Bis(2-carboxyethyl)-5-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and Alexa Fluor 488 and 568–conjugated goat anti-mouse and antirabbit secondary antibodies were from Invitrogen (Carlsbad, CA). Mouse monoclonal antibodies to the HA epitope were from Covance Research Products (Princeton ...
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bioRxiv - Biophysics 2022Quote: The lipid conjugated pH sensor PE-pHrodo was synthesized by linking DOPE to the pHrodo red ester reagent (Invitrogen, Eugene, OR, USA). Synthesis ...
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bioRxiv - Immunology 2022Quote: ... neonatal HSC or LMDPs sorted from the UCB were labeled with proliferation-dependent dye carboxyfluorescein diacetate succinimidyl ester (CFSE) using the Vybrant® CFDA SE Cell Tracer Kit (Life Technologies) prior injection to irradiated NSG mice (2 x 105 cells/mouse) ...
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bioRxiv - Synthetic Biology 2023Quote: ... DBCO-bicones were prepared by reacting purified bicones with Alexa Fluor 647-succinimidyl ester (Invitrogen, prepared as 10 mM stock in DMSO) and dibenzocyclooctyne-sulfo-N-hydroxysuccinimide ester (DBCO-Sulfo-NHS ester ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Immunology 2023Quote: ... Splenocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ and stained with Dylight650-E4 anti-Ars/A1 idiotype (E4 was produced and conjugated to DyLight™ 650 NHS Ester (Fisher Scientific) in our laboratory) ...
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bioRxiv - Biophysics 2022Quote: ... EVs were stained with 5-(and-6)-Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE, hereinafter referred as CFSE) (Thermo Fisher, Catalog No. C1157) and separated as described previously [10] ...
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bioRxiv - Biochemistry 2023Quote: ... Promastigote cultures were counted using a hemocytometer and resuspended in DPBS after washing twice (also in DPBS) then labelled with carboxyfluorescein succinimidyl ester (CFSE, CellTrace™ CFSE Cell Proliferation Kit, Cat# C34554,Thermofisher Scientific) in DPBS (1:1000) ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ and stained with Dylight650-E4 anti-Ars/A1 idiotype (E4 was produced and conjugated to DyLight™ 650 NHS Ester (Pierce/Thermo Scientific) in our laboratory) ...
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bioRxiv - Immunology 2024Quote: ... 1 mm3 burns were made with a cauterizer and the injury site was then stained with 10 µl of pHrodo Red succinimidyl ester (SE) (4 µM; Thermo Fisher Scientific). The incision was stitched ...
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bioRxiv - Molecular Biology 2024Quote: 0.5 × 106 cells from HL-60 were harvested by centrifugation and resuspended in PBS 200 μL with 10 μM 5-(and-6)-chloromethyl-2′,7′—containing Acetyl ester of dichlorodihydrofluorescein diacetate (H2 -DCF, Eugene, Molecular Probes, OR) and 0.4 μg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: Alexa Fluor™ 488 NHS ester (AF488)-labeled BAX was generated according to the manufacturer’s instructions (Thermo Fisher Scientific, Cat. No. A20000) at a 20:1 dye:protein molar excess ...
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bioRxiv - Cancer Biology 2024Quote: The generation of reactive oxygen species (ROS) was quantified with the fluorogenic probe CM- H2DCFDA (6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate, acetyl ester) (Thermo Fisher Scientific). Cells were treated with inhibitors for 4h ...
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bioRxiv - Cell Biology 2024Quote: The influenza A/WSN/33 (H1N1) virus was labeled with Alexa Fluor 594-NHS ester dye (A37572, Thermo Fisher, Carlsbad, CA, USA) as follows ...
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bioRxiv - Microbiology 2020Quote: ... The UPLC system consisted of 0.1% formic acid (mobile phase A) in ultrapure water and a mobile phase B consisting of 0.1% formic acid (Fisher Scientific, Loughborough, UK) in acetonitrile ...
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bioRxiv - Genetics 2022Quote: ... FRAP was performed on 3T3-L1 preadipocytes transiently transfected with GFP-tagged CIDEC were incubated with 200 μM of oleic acid and stained with Bodipy 558/568 C12 fatty acid (Molecular Probes, D3835) for 15 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... was captured using a GFP binding protein (GBP) covalently conjugated to carboxylic acid decorated Dynabeads (Dynabeads M-270 carboxylic acid, Thermo Fisher Scientific). Untagged SMO (Fig ...
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bioRxiv - Biophysics 2021Quote: ... The nucleic acid-specific fluorochrome SYTO 13 green fluorescent nucleic acid stain in 5 mM solution in DMSO (Molecular Probes, Eugene, OR) was used in all experiments ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were then stained for 1 minute in 5% Phosphomolybdic/Phosphotungstic acid solution and immediately transferred to 2.5% light green SF yellowish in 2.5% acetic acid (Fisher Scientific, cat# A38S-500) for 4 minutes ...
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bioRxiv - Microbiology 2024Quote: Total nucleic acids were extracted from 200 μL of inoculated viral transport medium using the MagMax™ Express 96 Nucleic Acid Extractor (Applied Biosystems) and the MagMax Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in Dulbecco’s modified Eagle’s minimum essential medium supplemented with 20% heat-inactivated fetal bovine serum and 1% non-essential amino acids (Gibco, Waltham, MA), and cultured in 10% CO2/90% air ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped by adding 35 μl of 1 M sulfuric acid (H2SO4) and the optical density was measured at a wavelength of 450 (OD450) (MULTISKAN FC, Thermo scientific).
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bioRxiv - Developmental Biology 2021Quote: ... The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain, Thermo Scientific). Each lane from the gel was cut and placed in 1.5 ml tubes ...
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bioRxiv - Immunology 2022Quote: ... L981F amino acid changed compared to WA-1) or S variant genes (Wang et al., 2021a) using Lipofectamine 3000 transfection reagent (ThermoFisher, CA). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 30 to 50 μl of cell lysate was digested using a 5:1 mixture of nitric acid (OPTIMA grade, 70%, Fisher Scientific) and ultrapure hydrogen peroxide (ULTREX II ...
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bioRxiv - Molecular Biology 2021Quote: ... the gels were fixed by 40% ethanol/10% acetic acid for 1 hr and stained overnight in 200 mL of Krypton Fluorescent gel stain (Life Technologies) diluted 1/10 in water ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS and cultured with amino acid free DMEM (GENAXXON bioscience) containing 1% penicillin-streptomycin and dialyzed FBS (Thermo Scientific) if indicated ...
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bioRxiv - Biochemistry 2020Quote: Samples were dissolved in water containing 10% methanol and 1% acetic acid and were subjected to ESI-mass spectrometry using a Q-Exactive mass spectrometer (Thermo scientific) by infusing through heated electrospray ionization (HESI ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed once with excess FACS buffer and resuspended in 500µl of 1:2000 diluted Sytox Blue nucleic acid stain (Thermo Fisher #S11348) in FACS buffer ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were incubated with the compounds for 72 hrs before adding SYBR-gold nucleic acid stain (1:10000 dilution) (ThermoFisher Scientific) modified from 72 substituting SYBR-green for SYBR-gold ...
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bioRxiv - Microbiology 2021Quote: ... Extracted DNA was visualized on a 1% agarose gel using Gel Red nucleic acid stain and quantified using Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and the NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were resuspended in 1 ml trace-metal grade 35% nitric acid (HNO3) and digested at 90°C for 1 hour in a high-density polyethylene scintillation vial (Fisher Scientific). Digested bacterial cells were diluted at a ratio of 1:10 in reagent-grade water prior to analysis ...