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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... non-sun-exposed skin as previously described(13) and used at passages 2-6 and grown in Epilife medium (Gibco, #MEPI500CA) with added human keratinocyte growth supplement (10ul/ml medium) ...
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bioRxiv - Immunology 2023Quote: ... All samples were assayed using the 7300HT or QuantStudio 6 Fast Real-Time PCR System and analyzed using Fast System Software (Applied Biosystems). All real-time PCR data was normalized to the level of GAPDH mRNA ...
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bioRxiv - Bioengineering 2024Quote: A volume of 4 μl of Cy5-labeled DO structures at 50 nM concentration were mixed with 6 μl R Buffer solution (Neon kits-MPK1096 Thermo Fisher). DO structures in R buffer were then immediately subjected to electroporation using the Neon Transfection system (MPK5000 ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs with the initial passage number of 24 were cultured in a 6-well plate coated with 0.5 mg Matrigel™ (354230, Fisher Scientific) in complete mTeSR plus medium (100-0276 ...
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bioRxiv - Cell Biology 2024Quote: ... E0771 cells were harvested by trypsinization and cultured at a density of 106 cells/well in 6-well NunclonTM SpheraTM low-adhesion plates (ThermoFisher, 174932) to prevent substrate attachment ...
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bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human embryonic stem cell line H9 was cultured on the 6-well plate coated with the Vitronectin (5 μg/ml) and in the Essential 8 Flex medium (both Thermo Fisher). Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue specimens were stained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min and mounted in VECTASHIELD Vibrance Antifade Mounting Medium (ThermoFisher Scientific). Akoya Biosciences’ PhenoImager HT (formerly known as Vectra Polaris Automated Quantitative Pathology Imaging System ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 10 nM of siRNA sequences (6-well plates) by using the Lipofectamine RNAiMax system (Thermo Fisher Scientific). Hypoxia was performed as previously described (24) ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR assays were performed in 20 μL reaction volumes using a QuantStudio 6 flex Real-time PCR system (Applied Biosystems). The primers used are listed in Supplemental Table S1.
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were detected and cover slips were attached using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) containing Prolong Diamond Anti-Fade (Life Technologies). Slides were imaged on a Keyence BX-X810 microscope.
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bioRxiv - Neuroscience 2023Quote: ... HBMECs were seeded in 6-well plates and at 75% confluency were stained for 20 min at RT using a crystal violet (Fisher Scientific) fixing (37% formaldehyde ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated from cultured mouse neurons treated with xcTauOs (250 nM total tau) or vehicle for 6 hours using the mirVana isolation kit (Invitrogen, AM1560). RNA integrity was evaluated by capillary electrophoresis using an Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... A final wash with PBS was performed before mounting the cells in ProLong® Gold Antifade Mounting medium with DAPI (4’,6-diamidine-2-phenylindole)(Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... and stained the same day of the experiment with the fluorescent dye 5(6)-TAMRA/SE™ (Thermo Fisher Scientific, C1171) at a final concentration of 1 μM (as in Wehrl et al ...
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bioRxiv - Physiology 2023Quote: ... 36 hours upon transfection DMEM-F12 medium in 6-well plates was replaced with fresh DMEM/F-12 medium without phenol red (Gibco #21041025). 48 hours post transfection ...
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bioRxiv - Cell Biology 2023Quote: N2A cells were seeded at 300,000 cells per well in a 6 well plate 24 hours before transfection using Lipofectamine 3000 (Thermo Fisher Scientific). Transfections were performed in accordance with the Lipofectamine 3000 protocol ...
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bioRxiv - Plant Biology 2023Quote: Heat-stress (HS)/LaCl3 experiments: 6-day old cells (20 mL in 100 mL flask) were treated with LaCl3 (Thermo Scientific) (750 µM ...
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bioRxiv - Genetics 2023Quote: Whole cell extracts of 2D cultures from one well of a 6-well plate were prepared using RIPA Lysis and Extraction Buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Synthetic cDNA encoding a self-cleaving polyprotein Bcl-6.t2A.Blc-xL to express the germinal center B cell-associated transcription factors Bcl-6 and Bcl-xL was synthesized (B6L; Integrated DNA Technologies) and cloned in pENTR/D-TOPO (Thermo Fisher), generating pENTR.B6L (35 ...
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bioRxiv - Immunology 2023Quote: ... a total of 6 – 9 consecutive 10-µm coronal sections were taken from of 4 – 6 equally distanced regions along the length of the spinal cord using a Shandon Finesse 325 microtome (Thermo Scientific). Sections were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The grids were blotted with filter paper for 6 s and immediately plunged into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) at 4 °C and 95% humidity ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg plasmid DNA per well was transfected into cells for 16 h at a confluency of 60% in 6-well plates using Lipofectamine 3000 (Invitrogen, L3000001) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... anti-mouse IL-1β biotin conjugate (13-7112-81), and anti-mouse IL-6 (MP5-20F3, 14-7061-85) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by medium-speed sedimentation at 10,000xg for 15 min using a fixed angle FIBERlite F14-6 x 500 y rotor (Thermo Fisher Scientific). The supernatant fraction was then centrifuged at 32,000 RPM for 1.25 h in a SW32 rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... reactions were performed using HOT FIREPOL EvaGreen Supermix (Solis BioDyne) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Every sample was analyzed in triplicate ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed using the PTFE filters and resuspended in 1x PBS or further biotinylated with 6 mg/ml EZ-link™ Sulfo-NHS-SS-biotin (#21331; ThermoFisher). Streptavidin conjugated to either AlexaFluor-488 or -568 was then added for 1h RT incubation ...
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bioRxiv - Immunology 2023Quote: ... Monocyte-derived macrophages (MDM) were generated by seeding three million CD14+ cells into one 6-well plate (NuncTM Thermo Fisher Scientific) in IMDM (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... Growth medium on HEK293T cells at 90% confluency in 6 well plates was replaced with Opti-MEM reduced serum medium (Thermo Fisher) and cells in each well were transfected with 250ng each of 8 pDUAL reverse genetics plasmids ...
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... mNat2 mRNA expression levels were determined by quantitative polymerase chain reaction (qPCR) using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). The cDNA was amplified using primers for mNat2 variant 2 and mGAPDH (see Supplementary Table S5 for details ...
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bioRxiv - Molecular Biology 2023Quote: ... SVG-A Notch2-HaloTag cells were transfected in 6 well plate format with 2 µg of DNA per well using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR assays were performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). The TaqMan Universal PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse-transcription-PCR (qRT-PCR) was performed on a QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems A43182) in 384-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... The expression levels of selected genes were determined using qRT-PCR (QuantStudio 6, ThemoFisher Scientific) using the SYBR Green Real-time PCR master mix (Thermo Scientific). The sequence of the primers used for the gene expression analysis was given in an appendix.
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bioRxiv - Cancer Biology 2023Quote: ... The B6-LSL-Cas9-eGFP keratinocytes were transiently transfected with 1μg of a CRE-expressing plasmid in 6-wells using Lipofectamine 2000 (Invitrogen; 11668-027) to activate Cas9-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were transfected with PNKP-FLAG (WT and mutants) expressing vector (pcDNA3) in 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed in technical quadruplets on a QuantStudio 6 Flex Real-Time 384-well PCR System (Applied Biosystems, 4485694) using PowerUp SYBR Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and then starved in 50 µl EMEM-Serum free medium (antibiotic free) in presence or absence of a protein kinase inhibitor (PKI) (6-22amide, 50 nM, GIBCO BRL) for 3 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Microbiology 2023Quote: Primary human macrophages were generated by differentiating blood-derived CD14+ monocytes for 6 days in Roswell Park Memorial Institute (RPMI)-1640 medium (ThermoFisher Scientific) containing 10% HyClone FBS (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... cDNA from samples was mixed with QuantiTect probe PCR master mix and gene-specific primers to run on a QuantStudio 6 Flex real-time cycler (Applied Biosystems). To generate a control for the amplification reaction ...