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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... The preparations were maintained at 37°C and stained with a bolus of voltage sensitive dye (0.25 ml, 10 μM Di 4 ANEPPS, Molecular Probes). Blebbistatin (7 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were centrifuged at 14,000 rpm for 10 min 4°C and supernatants were collected for BCA assaying (ThermoFisher Scientific) to determine protein concentrations ...
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bioRxiv - Biochemistry 2019Quote: ... Cell lysates were cleared of debris and nuclei via centrifugation at 42,000 x g (10,000 rpm) at 4°C for 10 min in a Fiberlite F21 rotor (ThermoFisher, cat.# 46923). The supernatant was layered directly onto a high-salt sucrose cushion (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein (10-30 μg) were separated on 4-12% Bolt bis-tris plus gels (Thermo Fisher Scientific) and transferred to Immobilon FL PVDF membranes (Millipore Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... and the samples were boiled for 10 minutes before being separated by electrophoresis using 4 – 12% NuPAGE Bis-Tris gel (Invitrogen). The proteins were then transferred into a PVDF membrane (iBlot 2 Transfer Stacks ...
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bioRxiv - Biochemistry 2021Quote: ... 10-30 µg of samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Insoluble material was clarified for 10 min at 4°C at 16k rcf and incubated with 60 μl Dynabeads HIS-Tag isolation (10104D, Invitrogen) for 2 hour with rotation at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin was disrupted by sonication, cleared by centrifugation (20,000 RCF, 10 minutes, 4°C) and lysates transferred to cryovials (ThermoFisher 374081) and stored in vapor phase of an LN2 tank ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of protein were electrophoresed on either 4-12% Bis-Tris or 10% Bis-Tris gels (NuPAGE, Life Technologies) and wet-transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 95 °Cfor 10 min and separated on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were transferred to PVDF membranes (EMD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads eluate was heated at 95°C for 10 min and loaded on polyacrylamide gel (4-12% Bis-Tris gel (Invitrogen)) and run in MOPS SDS Running Buffer (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... The same amount of sample (10 or 30 ng/ml) were loaded onto 4-15% pre-cast Bis-tris gel (Invitrogen) and run at 100V ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were size-fractionated on precast 10 cm 4-12% Bis-Tris or Tris-acetate gels (Thermo Fisher Catalog #NP0336BOX), then electroblotted onto nitrocellulose membranes (Thermo Fisher Catalog #88025) ...
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bioRxiv - Biochemistry 2022Quote: ... 500 μl fractions were collected and 10 μl of the sample of the fractions was taken for analysis on NuPAGE 4%-12% Bis-Tris gel (Invitrogen). Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and the supernatant centrifuged twice at 1000g for 10 min at 4°C (Sorvall RC-6 Plus Centrifuge, Thermo Scientific). The supernatant was centrifuged 8000g for 10 min at 4°C and then aspirated without disturbing the pellet ...
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bioRxiv - Physiology 2022Quote: ... and equal amounts (10 μg) were loaded into each lane and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Neuroscience 2022Quote: 10 μl of the cleared cell lysate was loaded onto a 4-12% Bis-Tris pre-cast gel (ThermoFisher NW04120BOX) and transferred onto a nitrocellulose membrane using the iBlot 2 dry blotting system (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: Glp1r-Cre;Ai9 mice were transcardially perfused with chilled 0.9% saline followed by 10% neutral buffered formalin (catalog #SF100-4, Fisher Scientific). Brains were removed and post-fixed 24h at 4°C in 30% sucrose in 10% buffered formalin ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times for 20 minutes each time in 1x PBS/0.3% Triton X-100/10% NDS at room temperature and incubated overnight at 4°C with secondary antibodies (Thermo Fisher Scientific Cat# A-21206 ...
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bioRxiv - Neuroscience 2022Quote: 10 μL of normalized lysate with loading buffer was loaded into NuPAGE 4 to 12% Bis-Tris protein gels (Invitrogen) and subjected to 100 V electrophoresis for 65 min ...
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bioRxiv - Immunology 2022Quote: ... was dialyzed for 48 h at 4°C into PBS using 10 kDa MWCO Slide-a-lyzer cassettes (Thermo Fisher) before i.n ...
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bioRxiv - Systems Biology 2023Quote: ... and 10-15 µg of protein were loaded on 4-12% Bis-Tris Midi NuPage Protein Gels (Thermo Fisher Scientific) and separated at 120V for 2 h in MES running buffer ...
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bioRxiv - Physiology 2024Quote: ... Slides were then washed 4 x 10 min in PBS and incubated with Goat anti-Rabbit 750 (A21039, Thermo Fisher) and DAPI in 3% BSA in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... for 10 minutes at 70°C before loading on a Bolt Bis-Tris Plus 4 to 12% gel (ThermoFisher Scientific) for total protein staining or Western blotting ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed for 10 min by 4% paraformaldehyde (PFA) diluted in Dulbecco’s PBS containing Ca2+ and Mg2+ (DPBS; Gibco 14040083), then blocked and permeabilized for 1 h in blocking buffer (10% bovine calf serum and 0.1% Triton X-100 in DPBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... 20-day-old female flies were dissected and fixated for 10 min in 4% paraformaldehyde in 1xPBS (Thermo Fisher Scientific). Primary antibodies were diluted in PBST ...
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bioRxiv - Cell Biology 2024Quote: ... and pCRSIPR-Lenti-V2 by electroporation using the NEON system (Thermo Fisher, buffer N, 4 pulses, 10 ms, 1230 V). After electroporation ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were spread onto superfrost plus microscope slides (Epredia) at 2,000 rpm for 10 min by Cytospin 4 (Thermo Scientific) or Cellspin 1 (Tharmac) ...
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bioRxiv - Cancer Biology 2024Quote: ... then equal quantities of protein lysate (3-10 µg) were separated by SDS-PAGE using 4–12% gradient gels (Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Microbiology 2024Quote: ... we pelleted samples by centrifugation at 5,000 x g for 10 minutes at 4°C and then resuspended the pellets in RNAlater (Invitrogen AM7021); samples were incubated in RNAlater at 4°C for 24 h ...
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bioRxiv - Immunology 2024Quote: ... centrifuged at 500g for 10 minutes at 4°C and resuspended in 0.25% Trypsin-EDTA (Thermo Fisher Scientific, #25200-072) at 37°C for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was centrifuged at 10000 g at 4°C for 10 minutes and supernatant was transferred to Dynabeads M-270 (Invitrogen) coupled to either CTD Ser2P (C15200005 ...
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bioRxiv - Zoology 2024Quote: ... We tested fixation using 4% Paraformaldehyde in 0.1M phosphate buffer (pH 7.4) (Bioenno LifeSciences, CA) and 10% Buffered Formalin Phosphate (Fisher Scientific, ON) with different fixation times and temperatures (Supplementary Table 1).
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 4 million cells were plated in 10 cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed for 10 min in 4% PFA and were acquired on an Attune NxT flow cytometer (Life Technologies). Results were analyzed with the FlowJo V10 software ...
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bioRxiv - Bioengineering 2024Quote: Organoids cultured for 63 days in static and dynamic conditions were stained with 10 µM of Fluo-4 AM cell permeant dye (F14201, ThermoFisher) for calcium imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-30ug of whole cell lysate was loaded into each well of 4-12% Bis-Tris NuPage polyacrylamide gels (Invitrogen). Proteins were transferred overnight onto PVDF membranes ...
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bioRxiv - Biochemistry 2023Quote: ... INPUT and UNBOUND (both 10 % of initial protein extract) and BOUND (IP) fraction were resolved by electrophoresis on acrylamide gels (4-12% Invitrogen) and the transferred onto nitrocellulose membrane that were saturated for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were blocked in 10% goat serum for 45 min and incubated with primary antibodies at 4°C overnight and secondary antibodies (Invitrogen) for 1 h at 37°C.
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble fraction was removed via centrifugation (13,000xg, 10 min, 4 °C) and protein abundance was quantified by BCA Protein Assay (Thermo Scientific). 50 μg of protein was utilized per well in a total volume of 60 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Transfected cells were incubated with a total of 4 µg DNA and 10 µL Lipofectamine 2000 (11668019, Thermo Fisher Scientific) complex for 72 hours and selected for another 14 days with 300µg/mL Zeocin ...
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bioRxiv - Immunology 2023Quote: ... added with 50 mM DTT for 10 min at 75 C and loaded onto precast NuPAGETM 4-12% bis-tris protein gels (Invitrogen), or NuPAGETM 12% bis-tris protein gels (these ones were used for LC3 immunoblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of the eluted proteins were separated by migration on a NuPAGE 4–12% Bis- Tris Protein Gel (Invitrogen) and silver stained ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed 4× for 5 min in PBS and either mounted with Vectashield with DAPI (Vector, H-1200-10) or fixed in 4% PFA (Thermofisher) in PBS for 20 min at room temperature to be processed for immuno-FISH staining.
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then heated at 100°C for 10 min and loaded onto a 1.5 mm NuPAGE gradient (4-12%) gel (Invitrogen, NP0336BOX). After electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of eluted proteins were briefly run on SDS-PAGE (4 min at 160 V, 10% SDS gel [NP0301, Invitrogen]), stained with SimplyBlue SafeStain (LC6065 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated for 10 min at 100°C and loaded onto a 1.5 mm NuPAGE gradient (4-12%) gel (Invitrogen, NP0336BOX). After electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... penicillin/streptomycin (10 μg/mL) and supplemented with the cytokines IL-4 (500 U/mL) and GMCSF (800 U/mL) (both Gibco) [42] ...
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bioRxiv - Microbiology 2023Quote: Four milliliters of each resuspension were pelleted via centrifugation (14,000 g, 10 min, 4 °C) (Heraeus Fresco 21, Thermo Scientific), the cells washed 4 times with ice-cold 0.9% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated on precast Novex 10% Tris-Glycine gel / NuPAGE 4-12% Bis-Tris gel at 100V using the Mini Gel Tank (Invitrogen) and were blotted onto PVDF membrane at 20V for 90 min ...