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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107, lot GR3234832-4; Donkey anti-Rabbit 594nm 1:1000, Invitrogen A21207 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated overnight at 4 °C with the following antibody: ERα (Dilution 1:1 µg/mL; Invitrogen, CAT: MA5-13191). IRDye secondary antibody (Dilution 1:20,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275; Phosphorylated-cofilin 1:500 Invitrogen #44-1072G and β-actin 1:3000 ...
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bioRxiv - Immunology 2023Quote: ... The upper aqueous layer was removed to a new tube and mixed 1:1 with Isopropanol (Fisher Scientific, BP2618-4) and incubated for 1 min with glycoblue co-precipitant (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1.2 M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 µg/mL DAPI (4’, 6-diamidino2-phenylindole; Molecular Probes). Cells were imaged with a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... at an OD600 = 10 and 1% Luria-Bertani (LB) agar was poured onto a 9 cm diameter Petri dish (Fisher Scientific, Waltham, MA, USA). While the agar solidified ...
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bioRxiv - Biochemistry 2022Quote: ... The quality of obtained microsomes was tested with 9-amino-6-chloro-2-methoxyacridine (ACMA; Invitrogen A1324) fluorescence quenching assays.
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bioRxiv - Immunology 2022Quote: ... Harvested naïve CD4+ T cells were plated at a density of 1.5 × 105 cells/mL in 2 ml of complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies], and 0.05% (50μM) 2-ME [Sigma-Aldrich]) ...
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bioRxiv - Neuroscience 2020Quote: ... Three hours after plating medium was exchanged for maintenance medium (Neurobasal-A without Phenol Red, 2% B-27 supplement, 1% penicillin-streptomycin, 0.5 mM glutaMAX, 25 μM β-mercaptoethanol; ThermoFisher Scientific) and cells were kept at 37°C ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using ketamine / xylazine and intraviteally injected with 2 μl AF488 cholera toxin subunit B (1 mg/ml in PBS) (ThermoFisher Scientific). After 72 h mice were anaesthetized and euthanized via 4% PFA cardiac perfusion ...
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bioRxiv - Neuroscience 2023Quote: ... then the wells were filled with 1 mL Neurobasal™ Plus Medium supplemented with 2% B-27 Plus supplement and 0.5% GlutaMAX™ all from ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Neuroscience 2022Quote: ... washed with PBST (2×10 min) and DAPI-mixed (1:10000, Thermo Scientific) PBS solution (1×20 min) ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... beads were washed five times with RIP buffer for 5 min at 4°C and RNA was extracted using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from WT and MafAS64F/+ islets (n=4 for males, n=5 for females) using the RNAqueous total RNA isolation kit (Ambion; Thermo Fisher), and then analyzed on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Genomics 2019Quote: ... boiled in 2X NuPAGE LDS Sample Buffer (including 1.8% β-Mercaptoethanol) for 5 min and separated in NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific).
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bioRxiv - Genomics 2020Quote: ... the following components were added: 4 μl of 5× RT buffer (from the Maxima H Minus Reverse Transcriptase kit, Thermo Fisher Scientific), 1 μl of RNaseOUT™ (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was used for the measurement of IFN-β levels while the cell pellet was resuspended in PBS and smeared using Cytospin 4 Cytocentrifuge at 400 x g for 5 min (Thermo Fisher Scientific). The cells were stained with Diff-Quik (Sysmex ...
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bioRxiv - Biochemistry 2021Quote: ... and then heated at 95 °C for 5 min before loading onto a pre-cast 4-12 % Bis-Tris polyacrylamide gel (Thermo Fisher Scientific). Samples were run at 200 V for 45 min before staining with Coomassie Blue.
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bioRxiv - Neuroscience 2020Quote: ... To elute samples the beads were then boiled in SDS sample buffer for 5 mins and run on a 4-12% Bis-Tris gel (Invitrogen, Cat# NW04127BOX). Rabbit GluR2 polyclonal antibody (Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were heated at 95 °C for 5 min and run on a 4-12% Bis-Tris NuPage gel (Thermo Fisher, #NP0321). Samples were then transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... with or without inhibitors at 4°C for 5 min and 37°C for 30 min in the presence of FM4-64FX (Thermo Fisher Scientific) to stain wounded cells ...
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bioRxiv - Bioengineering 2022Quote: ... 40 µg total protein from planarian lysate or 5 µg total protein from HeLa cell lysate were loaded per well onto a NuPAGE 4-20 % BisTris gel (ThermoFisher, Cat#NP0321BOX) and run in 1× MOPS buffer (ThermoFisher ...
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bioRxiv - Physiology 2022Quote: 60 x 106 of mouse splenocytes were incubated for 15 min at 4°C in PBS containing 5% Normal Rat Serum (Thermo Scientific, 10710C) and anti-CD16/32 (BioLegend ...
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bioRxiv - Genomics 2021Quote: ... One OD600 absorbance unit (1.25 - 2.5 ml) of cells were collected and lysed at 95°C in 15 µL 4× NuPage LDS Sample Buffer (Thermo Fisher Scientific) with 5% β-mercaptoethanol for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... purified and ligated into the expression vectors pcDNA™4/TO (Orco) and pcDNA™5/TO (ORs) (all Thermo Fisher Scientific), followed by transformation into HB101 competent cells (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 72°C for 5 minutes and then held at 4°C in a Veriti™ 96-well thermal cycler (Applied Biosystems™). Adaptors from the LSK-109 sequencing kit (Nanopore ...
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bioRxiv - Biophysics 2019Quote: ... heated at 100°C for 5 min and run on a 4 to 12% Bis-Tris SDS-polyacrylamide gel (Life Technologies, CA). Loading amounts of proteins were 1 μg for Coomassie staining and 0.5 μg – for Western Blot ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared at 16,000g for 15 min at 4 °C and supernatants were supplemented with 5 μl/ml of RNase A/T1 (Thermo Fisher Scientific) for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured neurons were transfected with plasmids (1.5 μg of plasmid DNA per well) at 4 - 5 d in vitro (DIV) using a commercial calcium phosphate transfection kit (Thermo Fisher Scientific) as previously described21,24.
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were washed twice with cold phosphate-buffered saline and lysed at 4 °C (on ice) in RIPA buffer (Wako) containing 5 mM EDTA and protease inhibitor cocktail (Thermo Fisher Scientific). The cell lysates were cleared by centrifugation ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were collected by centrifuging 5 min at 300 g and 4°C and resuspended in PBS containing 0.04% BSA (Thermo Fisher Scientific, #AM2616). The cell suspension was filtered to remove cell aggregates and particles with a diameter greater than 35 µm ...
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bioRxiv - Biophysics 2019Quote: ... in vitro (DIVs) 3D neuronal cultures were loaded with the fluorescent calcium indicator Fluo-4 AM (5 µM) (F14201, Thermo Fisher Scientific), in extracellular recording solution (95 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μg protein from each sample was fractionated on a 4-12% Bis-Tris SDS-PAGE gel (Life Technologies/ Thermo Fisher Scientific), then electrotransferred to nitrocellulose ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μg protein from each sample was fractionated on a 4-12% Bis-Tris SDS-PAGE gel (Life Technologies/ Thermo Fisher Scientific), then electrotransferred to nitrocellulose ...
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bioRxiv - Physiology 2021Quote: Platelets were isolated from mice 24 hours after injection with anti-CD42c-DyLight-x649 and were subsequently incubated with Fluo-4 AM (5 μM; Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor488-labeled human transferrin was dissolved in PBS 5 at mg/ml and stored at 4°C (Thermo Fisher, ref. no. 13342). TexasRed-labelled neutral 3’000 and 40’000 Da dextran was dissolved in PBS at 10 mg/ml and stored at -20°C (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: 3D caecaloids grown in expansion medium for 4-5 days after passaging were dissociated into single cells by TrypLE Express (Gibco Thermo Fisher Scientific) digestion ...
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bioRxiv - Microbiology 2022Quote: ... to approximately 5 µl and 4 µl were transformed into 100 µl of ElectroMax DH5α-E Competent Cells (Cat# 11319019, Thermo Fisher Scientific). After 30 min of recovery at 37°C in 4 ml of SOC medium ...
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bioRxiv - Neuroscience 2022Quote: ... 1% Triton X-100 and 5% goat serum and incubated overnight at 4°C and DNA was labeled using Hoechst (Thermo Fisher, H3570). Images were subsequently captured with confocal microscope ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... (3) 65%-95% for 5 min and (4) washout for 15 min) using the EASY-nano LC 1200 system (Thermo Fisher Scientific) with a flow rate of 300 nL/min ...
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bioRxiv - Cell Biology 2022Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 150V for 120 min and transferred to 0.45 μm PVDF membranes (Sigma ...