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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... a final extension step (68°C for 5 min) and holding the reaction at 4°C in a thermocycler (Applied Biosystems) with a preheated lid (105°C) ...
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bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the samples were run on a thermocycler (60 min, 37°C; 5 min, 95°C; held, 4°C; Applied Biosystems); cDNA was stored at −20°C until use.
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bioRxiv - Microbiology 2023Quote: ... Blocking was performed for 1h (5% skimmed milk in Tris-buffered saline containing 0.5% Tween) and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A ...
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bioRxiv - Cell Biology 2023Quote: Immunohistochemistry and Immunofluorescence were performed on 4% PFA-fixed paraffin-embedded 5-μm sections mounted onto Super frost microscope slides (ThermoFisher Scientific) or whole mounts of denuded oocytes ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated for 5 min at 99°C before loading on 4-12 % SDS-PAGE with MOPS as running buffer (Thermo-Fisher Life Technologies). BenchMark protein ladder was used as marker (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed again at 1224 rpm for 5 minutes at 4 degrees Celsius and then counted using an automated cell counter (Countess, Thermo Fisher) to obtain the cell concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300g for 5 minutes at 4°C and homogenized in cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) or cold RIPA lysis buffer (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... the media from each well was aspirated and the wells were overlaid with 4 ml/well DMEM with 5% FBS and 0.7% agarose (Invitrogen, 16500-500). At 7 dpi ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were washed again at 1224 rpm for 5 minutes at 4 degrees Celsius and then counted using an automated cell counter (Countess, Thermo Fisher) to obtain the cell concentrations ...
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bioRxiv - Biochemistry 2024Quote: ... Protein isolates were mixed with protein loading buffer containing 100 mM DTT reducing agent and denatured for 5 min at 95°C before loading onto 4–12% Bis-Tris NuPAGE precast gels (Thermo Fisher) for electrophoresis ...
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bioRxiv - Systems Biology 2024Quote: ... about 10 cm of leaf length were cut into small pieces and placed in chilled 5-mL tubes containing 4 mL of RNALater (Fisher Scientific) solution for RNA stabilization and storage ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cell suspension was passed through a 70-μm filter, centrifuged (400xg, 5 minutes, 4 °C) and resuspended in Advanced DMEM/F12 (ThermoFisher #12634010) supplemented with FCS (10% vol/vol ...
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bioRxiv - Neuroscience 2024Quote: ... The RNase I treatment was carried out as described by Ingolia et al.19 for 45 min at 4 °C using 5 µl of RNase I (Ambion, AM2295) per 250 µg of crude total RNA and quenched by adding 20 µl of SUPERase-In (Ambion ...
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bioRxiv - Developmental Biology 2021Quote: ... and quenched 1:5 in DMEM supplemented with 10% FBS (Life Technologies, 10270106). Cells were monthly tested for mycoplasma contamination by PCR.
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bioRxiv - Neuroscience 2024Quote: ... A 10-µl drop of 5 mM OGB-1 (hexapotassium salt; Life Technologies) in ACSF was suspended from the upper electrode and lowered onto the retina ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM EGTA) and coated with anti-biotin (5% in 1× SAB; Invitrogen), and the nonspecific surface was blocked with Pluronic F127 (1% in 1× MRB80 ...
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bioRxiv - Neuroscience 2021Quote: ... + 2% heat inactivated fetal bovine serum Certified One Shot (Gibco A38400-01) + 1× N-2 supplement ...
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bioRxiv - Genomics 2021Quote: ... on the Ion One Touch™ 2 System (Thermo Fisher Scientific, USA). Template-positive ISPs were enriched on Ion One Touch™ ES system (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2024Quote: ... coli One Shot™ ccdB Survival™ 2 T1R Competent Cells (ThermoFisher). Single colonies were cultured for plasmid isolation and the cloned sequence was confirmed by DNA sequencing.
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bioRxiv - Microbiology 2022Quote: ... coli One Shot® ccdB SurvivalTM 2 T1R competent cells (Life Technologies), which was grown in LB agar plates (10 g l-1 NaCl ...
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bioRxiv - Microbiology 2021Quote: ... BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene lipid stain was from Molecular Probes (Invitrogen/ Thermo Fisher Scientific).
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with primary antibodies (overnight, 4°C), washed (5X, PBS, 4°C) and incubated with species-specific secondary antibodies coupled to fluorophores (Thermo Fisher). Cells were then washed (5X ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were resolved for 4 hr at 100 V on 4% −8% polyacrylamide gradient Tris-acetate denaturing gels (Novex, Life Technologies) and transferred to PVDF membranes ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were ultracentrifuged for 4 hours at 50.000 rpm at 4°C in a Sorvall MX 120/150 Plus MicroUltracentrifuge (Thermo Fisher Scientific) in S55A2 rotor ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized using isofluorane and perfused transcardially with 4 mL PBS followed by 4 mL RNAlater™ (Invitrogen; CAT # AM7020) using a peristaltic pump at a flow rate of 0.8mL/min ...
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted at blotting force 20 for 4 s at 4°C and 100% humidity and plunged into liquid ethane using a Vitrobot (Thermo Scientific).
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bioRxiv - Immunology 2019Quote: ... 100 μg/mL streptomycin with either 110 U/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) (Leukine; Sanofi) and 282 U/mL interleukin-4 (IL-4) (Affymetrix, eBioscience) for DCs or 280 U/mL GM-CSF for macrophages ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cellular response in each well was measured via fluo-4 intracellular calcium dye using the fluo-4 NW calcium assay kit (Molecular Probes) according to manufacturer’s directions ...
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bioRxiv - Bioengineering 2019Quote: ... and CarboPac PA1 standard bore guard column (4 x 50 mm) plus CarboPac PA1 preparative IC column (4 x 250 mm, both Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2022Quote: Protein samples in 1X LDS sample buffer containing 4% β-mercaptoethanol (BME) were separated by size on 4-12% Novex NuPage Bis-Tris SPS-PAGE (Invitrogen) gels (2 h ...
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bioRxiv - Genetics 2022Quote: ... Larval brains were fixed in 25 ml of acetic acid: 4% formaldehyde in PBS (45%:55%) for 4 min on a clean Superfrost plus slide (Fisher Scientific) and the sample was manually squashed via thumb/stamp over coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, NW04122BOX) and transferred onto a PVDF membrane (Immobilon-FL ...
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bioRxiv - Neuroscience 2022Quote: ... Three successive 5-hour paraffin wax embedding stages were performed followed by cooling and sectioning of tissue on a Leica microtome into 4 × 4 μm thick serial sections that were collected on Superfrost (ThermoFisher Scientific) microscope slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Separation of anionic metabolites were performed using a Dionex IonPac AS11-HC-4 µm column (250 × 0.4 mm, 4 µm; Thermo Fisher Scientific). The injection volume was 0.4 µL ...
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bioRxiv - Cell Biology 2024Quote: ... Click-iT reaction cocktail (100 Mm Tris-buffered saline, 4 mM CuSO4, 100 mM ascorbic acid and 4 µM Alexa FluorTM488 azide (Invitrogen #A10266) was used for detecting EU ...
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bioRxiv - Neuroscience 2024Quote: ... larvae and dissected adult brains were fixed overnight in paraformaldehyde (PFA; 4% in 1x phosphate-buffered saline) at 4°C then dehydrated overnight in 100% methanol (A4124, Fisher Scientific) at -20°C ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... -4 blot force for 4 seconds and plunged frozen in liquid ethane using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected on a Titan Krios operating at 300 keV and equipped with a Gatan K3 direct detector and a 20 eV slit width energy filter ...
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bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... MBL) for 4 days at 4°C and with Alexa-Fluor (AF) 488 goat anti-rabbit IgG (R37116, Thermo Fisher Scientific) overnight at RT followed by DAPI staining (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 22,000 × g at 4°C for 20 minutes and poured over 4 mL of HisPur Cobolt resin (89964, Thermo Fisher Scientific). The slurry was rotated at 4°C for 1 hour and spun down at 700 × g for 2 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: A fraction of the liver from each mouse was preserved in 4% formalin (Cat# SF100-4, Thermo Fisher Scientific, Waltham, MA) followed by 70% ethanol for histology ...
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bioRxiv - Immunology 2023Quote: ... blotted for 4 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher). All grids were screened using a ThermoFisher Glacios microscope (OSU Center for Electron Microscopy and Analysis).
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bioRxiv - Cell Biology 2023Quote: ... Extracts were clarified by centrifugation twice for 15 minutes at 16,000 rcf at 4°C and 100 μl embryo extract was rotated at 4°C overnight with 30 μl magnetic ProteinA dynabeads (Life Technologies) coupled with anti-MBP antibodies (gift from Jordan Raff ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...