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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... An α-Mouse IgG2a-AF488 secondary antibody (Invitrogen, CAT#: A-21131; 1-4 hours, 25°C), cells were washed 3 times ...
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bioRxiv - Bioengineering 2020Quote: ... assessment of both live and dead cells on the N27 cell line was performed with a combination of 10 μM CellTracker green and 4 μM Propidium Iodide (PI, Invitrogen, ThermoFisher) solutions ...
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... were injected in the pancreas with 4 ml of collagenase solution (7 ml HBSS, 70 μL HEPES (10 μM) (Gibco), 16,5μL DNAse (10mg/mL ...
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bioRxiv - Genomics 2021Quote: ... and centrifuged for 10 min at 4°C and 1500 RPM on a Megafuge 40R Refrigerated Centrifuge (Thermo Scientific #75004518). CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μm thick vibratome sections were postfixed with 4% PFA for 15 min at RT followed by blocking in 10% normal horse serum (Gibco) and 0.2% Triton in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 10-30 μg of samples was loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 70 µm nylon colander (Falcon) with 3-4 ml of Ca2+- and Mg2+-free HBSS medium containing 10 % fetal bovine serum (FBS, Gibco), 1 % MEM-vit (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were then clarified through centrifugation at 10000 rpm for 10 min at 4°C and concentration determined measuring Absorbance at 280nm in the Nanodrop 1000 (ThermoFisher). Lysates containing 800μg of protein in a total of 400μl lysis buffer were pre-cleared by incubation with 15μl of Protein A magnetic beads (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were separated on a 4–12% gradient Bis-Tris gel (NuPAGE Bis-Tris gels, 1.0 mm, 10 well; Art. No. NP0321; Life Technologies) in 1X MOPS (NuPAGE 20X MOPS SDS running buffer ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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bioRxiv - Genetics 2019Quote: ... by incubating at 70°C for 10 minutes and separated on 4-12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific) along with 5% of input samples and analysed by Western blotting as described above.
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bioRxiv - Cancer Biology 2020Quote: ... Cell debris was pelleted by centrifugation at 13,000g for 10 minutes at 4°C and the protein concentration measured by bicinchoninic acid (BCA) assay (Pierce, Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 450 xg for 10 min at 4 °C and pellets were incubated with red blood cell lysis buffer (Invitrogen) for 30 sec ...
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bioRxiv - Cell Biology 2020Quote: ... Laemmli sample buffer (0.1 mL of 4x) was added to lysate prior to loading 10 μL on a 4%–12% Bis-Tris NuPAGE gel (Invitrogen). Gels were transferred onto a Mini-size polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (10-20 μg) were run on SDS/PAGE with NuPAGE precast 4-12% gradient gels (Invitrogen; Carlsbad, CA, USA) and blots were incubated overnight at 4°C with primary antibodies (Supplementary Table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates containing 10 μg of total protein were loaded (without boiling) on precast NuPAGE 4-12% Bis-Tris gels (Invitrogen) for SDS-PAGE analysis ...
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bioRxiv - Bioengineering 2021Quote: ... cocultured with macrophages in RPMI +10%FBS for 48 h and fixed afterwards for imaging using 4% paraformaldehyde (PFA, ThermoFisher). Cells were imaged using a DMI 6000B inverted phase contrast microscope (Leica Camera ...
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bioRxiv - Bioengineering 2020Quote: ... The next day samples were centrifuged at 13,000 rpm for 10 min at 4 °C (Thermo Scientific Sorvall Legend X1R) The supernatant was removed and placed in a speed-VAC (Savant SC110 ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 minutes at 70°C and then loaded into a 4-12% NuPAGE Bis-Tris gel (Invitrogen, NP0326BOX). After electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... 10-30 μg of samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% or 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF or P PVDF membranes (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were prepared with 10–30 μg of protein in RIPA buffer with 4× LDS loading buffer (LP0001, Life Technologies). Samples were electrophoresed on SDS/PAGE gels ...
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bioRxiv - Cell Biology 2021Quote: ... Protein lysates (10-15 mg, tissues) were then incubated overnight at 4 °C with antibody-coated dynabeads (Thermo Fisher Scientific). Bound immune complexes were washed twice with lysis buffer containing NP-40 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% v/v bromophenol blue in milli-Q water) and incubated at 95° C for 10 min prior to loading on a 4-12% Tris-Glycine gel (Invitrogen). For reducing SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... blotting force: 10) and plunge-frozen at 4°C under 95% humidity using Vitrobot Mark IV (Thermo Fisher Scientific, USA). The frozen grid was imaged using a Titan Krios G3 microscope at an acceleration voltage of 300 kV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde for 10 min at room temperature and coverslips were mounted on slides with ProLong Gold Antifade Mountant (Thermofisher) and imaged on an Olympus AX70 ...
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bioRxiv - Neuroscience 2019Quote: COS-7 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) and were transiently transfected with GluA1-4 expression plasmids using Lipofectamine 2000 (Invitrogen). After 48 h of transfection ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 μl of boiled supernatant of whole cell lysates was resolved on 4-12% Bis-Tris NuPAGE protein gel (Invitrogen) in 1×MES buffer (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... all the crosslinking samples were boiled for 10 min and resolved on a NuPAGE 4–12% gradient polyacrylamide gel (Invitrogen). Gels were stained using SimplyBlue (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated to 90°C for 10 min before being loaded on 4-12% Bis-Tris polyacrylamide gels (ThermoFisher). Each experiment was triplicated ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed with 4% formalin solution (neutrally buffered) for 10 minutes and kept in phosphate-buffered saline (PBS, no. 10010023, Gibco) during the experiment.
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bioRxiv - Plant Biology 2021Quote: Phosphopeptide enrichment was performed using a Fe3+-IMAC column (Propac IMAC-10 4×50 mm, Thermo Fisher Scientific, Waltham, USA) as described previously (Ruprecht et al. ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated at 95 °C for 10 min before loading onto a 4-12% gradient Bis-Tris mini-gel (Invitrogen). After separation ...
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bioRxiv - Neuroscience 2019Quote: Mice were terminally anaesthetised with intraperitoneal injection of pentobarbitone followed by thoracotomy and transcardial perfusion with 10 ml 0.9% NaCl followed by 20 ml 4% paraformaldehyde (PFA, Fisher Scientific) in phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2021Quote: ... Solubilised protein was cleared by centrifugation (10 min, 125000xg, 4°C) and incubated with antibodies cross-linked to Dynabeads (Invitrogen) by overhead rotation for 2 h on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads eluate was heated at 95°C for 10 min and loaded on acrylamide gel (4-12% Bis-Tris gel (Invitrogen)) and run in MOPS SDS Running Buffer (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated for 10 min at 90°C and loaded on a 4–20% Tris-Glycine precast gel (ThermoFisher). Protein was transferred onto a nitrocellulose membrane using the iBlot Dry Blotting System ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty μg of total protein extracts and 10 μg of nuclear extractions were loaded on 4-12% Bis-Tris gels (Invitrogen) in SDS-MES running buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... 10-40 μg of proteins were loaded on a precast gel NuPAGE Novex 4-12% Bis-Tris (Thermo Scientific, NP0336BOX). Proteins were then transferred onto a nitrocellulose membrane using an iBlot2 dry blotting system with iBlot2 transfer stacks (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 100 µg of mitochondria were pelleted at 10,000 x g for 10 minutes at 4°C and resuspended in 1x pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5 ...
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bioRxiv - Cell Biology 2022Quote: HTM hydrogels on coverslips subjected to the different treatments for 10 d were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at 4ºC overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with PBS and pelleted by centrifugation (450 x g, 10 min, 4 °C) prior to being stored at -80 °C in TRIzol Reagent (Invitrogen). RNA was extracted with the Direct-zol RNA Microprep kit as per manufacturer’s instructions (Zymo Research R2062) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were clarified by centrifugation at 20,800 g at 4°C for 10 min and supernatant protein concentrations quantified using the Bradford assay kit (ThermoFisher, #23236)
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bioRxiv - Molecular Biology 2020Quote: ... phosphopeptides were enriched from 1mg total protein digest using a ProPac IMAC-10 column (4×50mm) (Thermo Scientific, P.N. 063276). Loading buffer (A)-0.1% TFA ...
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bioRxiv - Cancer Biology 2019Quote: ... for 10 min at 70°C before they were separated on 4-12 % Bis-Tris polyacrylamide gels with MES buffer (Invitrogen), and transferred to a nitrocellulose membrane using the iBlot Dry Blotting System (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were fixed in 4% paraformaldehyde for 10 min to stop the reaction and mounted with ProLong Diamond Antifade Mountant containing DAPI (Invitrogen). Imaging was performed on a BZ-X800 microscope (Keyence) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 to 20 μg total protein was separated on a NuPAGE 4-12% Bis-Tris denaturing gel (Thermo Fisher Scientific) and transferred to a nitrocellulose membrane using an iBLot2 dry transfer (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Lysate was centrifuged for 10 min at 10,000 × g at 4°C and protein concentration in the supernatant measured by BCA assay (Thermo scientific). Lysate equivalent to 5 mg of total protein was incubated with 30 μl anti-myc magnetic beads (Thermo scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were obtained by centrifugation at 12000 g for 10 min at 4°C and incubated with goat-anti-Flag agarose beads (PA1-32374, Invitrogen) or proinsulin Ab (CCI-17 ...