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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Canine Parvovirus 2 VP2 Capsid Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The immuno-detection of proteins was carried out using mouse anti-HA monoclonal antibody (2-2.2.14, #26183, ThermoFisher Sci) and secondary anti-mouse IgG antibodies conjugated with horseradish peroxidase (#A9044 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed by adding 2 mg of P-CREB antibody to protein A/G Dynabeads (Life Technologies, USA) and incubating the mixture overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in PBS containing 0.05% Tween-20 and probed with anti-mono ADP-ribose binding reagent MABE1076 (α-MAR ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... Some of the precipitates were eluted in the mixture of IP Buffer and SDS sample buffer (2:1) to elute proteins and the others were eluted in TRIzol Reagent (Invitrogen) for RNA isolation.
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bioRxiv - Genetics 2020Quote: ... and 2 μg of plasmid encoding tethered NHA-protein using 5 μl of Lipofectamine 2000 and OPTI-MEM media (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 S.dCT were co-transfected into HEK293T cells using lipofectamine 2000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S and RBD proteins were transiently expressed in Freestyle 293F system (ThermoFisher). In brief ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Cancer Biology 2021Quote: ... 200-500 μg of nuclear lysates were incubated with 2 μg of specific antibodies and 50 μg of Dynabeads Protein G (Invitrogen). After overnight incubation on a rotator at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mg of total protein was incubated with streptavidin-conjugated magnetic beads (Dynabeads MyOne Streptavidin C1, Thermo Fisher Scientific) for 16 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein from the finished gel was transferred to a nitrocellulose membrane using an iBlot 2 Transfer stack (Invitrogen, Cat# IB23002). Membranes were washed in Odyssey Blocking buffer (LI-COR Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific). The supernatants containing the pseudotype viruses were collected 48 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... using MES/SDS running buffer prepared according to the manufacturer’s instructions and including pre-stained protein markers (SeeBlue Plus 2, ThermoFisher Scientific). Proteins were transferred to Amersham Protran nitrocellulose membranes (0.45 µm pore size ...
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bioRxiv - Synthetic Biology 2021Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µg input protein (typically < 2% total input protein) and 40% of IP eluates were loaded onto 4-12% Bis-Tris gradient gels (Invitrogen) and analyzed by immunoblotting.
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bioRxiv - Microbiology 2022Quote: ... BHK-21 were transfected to express the SARS-CoV-2 S protein using Lipofectamine 3000 (Thermo Fisher Scientific, Madrid, Spain) and after 24h transfected cells were inoculated with a replication-deficient rVSV-luc pseudotype (MOI ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... HEp-2 cells were transiently transfected with an expression vector of fluorescently labeled proteins using Lipofectamine LTX & Plus Reagent (Invitrogen) for 48 h before stress exposure ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Plant Biology 2021Quote: ... with a concentration of 2 μg / sample and incubated in ChIP dilution buffer with 20 μL of Dynabeads Protein A (Thermofisher) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... was added to 2 µg purified RBD protein in 18 µl PBS in a MicroAmp Fast 96-Well Reaction Plate (0.1ml) (Applied Biosystems), for a total reaction volume of 20 µl ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... They were then co-transfected with 500 ng of IRF3-GFP and 500 ng of SARS-CoV-2 protein-expressing plasmid using Lipofectamine 2000 reagent (11668019, Invitrogen). 24 hours post-transfection ...
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bioRxiv - Microbiology 2019Quote: ... were resolved by 10% SDS-PAGE and the proteins were transferred to a membrane by using a semi-drying transfer system (iBlot 2, Invitrogen). The membrane was then blocked with blocking solution containing 4% milk (dry skim milk dissolved in 20 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP purification was carried out as previously described 48 with 2 mg of protein extract and 50 μl of Dynabeads Pan Mouse IgG (Invitrogen) per sample ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated on an SDS-PAGE gel and transferred to a nitrocellulose membrane using iBlot 2 apparatus (ThermoFisher Scientific). The membrane was incubated in TBST (20mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... Beads were washed four times with PBS containing protease inhibitors and protein were labeled with 2 mM Alexa 488 C5 maleimide or Alexa 546 C5 maleimide (Invitrogen) overnight at 4 °C in the dark ...
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bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
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bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then washed and incubated for 2 hours at room temperature in 1:500 Streptavidin protein 488 (21832, Invitrogen). Images were captured using a fluorescence microscope with 1s exposure time and no change of contrast for all pictures to visualize various hypothalamic nuclei ...
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bioRxiv - Cancer Biology 2020Quote: ... SDS-PAGE was then performed and proteins transferred to PVDF membranes using the iBlot 2 transfer system (Thermo Fisher Scientific). Membranes were incubated for an hour in Rockland blocking buffer (Rockland ...
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bioRxiv - Microbiology 2020Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Microbiology 2020Quote: ... were resolved by 10% SDS-PAGE and the proteins were transferred to a PVDF membrane using a semi-dry transfer system (iBlot 2, Invitrogen). The membrane was then blocked overnight with blocking solution containing 4% milk (dry milk dissolved in 20 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Immunology 2020Quote: The prefusion ectodomain of the SARS-CoV-2 Spike protein was transiently transfected into suspension-adapted ExpiCHO cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples were resolved on a Bolt 4-12% Bis-Tris protein gel (invitrogen) and transferred to a nitrocellulose membrane with the iBlot 2 dry blotting system (Invitrogen). The following primary antibodies were used for western-blotting at 1/1,000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... PABP-GFP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-GFP (clones 19F7 and 19C8 ...
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bioRxiv - Molecular Biology 2022Quote: ... endogenous PABP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-PABP (clone 10E10 ...
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bioRxiv - Neuroscience 2022Quote: ... The bead-protein complexes were isolated by using a magnetic stand (DynaMag™-2 magnet, Cat# 12321D, Thermo Fisher Scientific) to isolate the pull-down fraction ...